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Protein

Glutathione peroxidase 1

Gene

Gpx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protects the hemoglobin in erythrocytes from oxidative breakdown.

Miscellaneous

In the absence of Sod1, Gpx1 in the liver undergoes a 40% reduction in catalytic activity as a result of the decomposition of Sec-47 to dehydroalanine.1 Publication

Caution

PubMed:2771650 sequence was originally thought to originate from human.Curated

Catalytic activityi

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Kineticsi

  1. KM=14 µM for H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  2. KM=29 µM for tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  1. Vmax=319 mM/min/mg enzyme toward H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  2. Vmax=182 mM/min/mg enzyme toward tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei471
Sitei47Subject to oxidation and hydroselenide loss to dehydroalanine1

GO - Molecular functioni

  • glutathione peroxidase activity Source: MGI
  • SH3 domain binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-MMU-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-MMU-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-74259 Purine catabolism
SABIO-RKiP11352

Protein family/group databases

PeroxiBasei3709 MmGPx01

Chemistry databases

SwissLipidsiSLP:000001635

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione peroxidase 1 (EC:1.11.1.9)
Short name:
GPx-1
Short name:
GSHPx-1
Alternative name(s):
Cellular glutathione peroxidase
Selenium-dependent glutathione peroxidase 1
Gene namesi
Name:Gpx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104887 Gpx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666131 – 201Glutathione peroxidase 1Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei32PhosphoserineBy similarity1
Modified residuei62N6-acetyllysine; alternateCombined sources1
Modified residuei62N6-succinyllysine; alternateCombined sources1
Modified residuei86N6-acetyllysine; alternateCombined sources1
Modified residuei86N6-succinyllysine; alternateCombined sources1
Modified residuei112N6-acetyllysine; alternateCombined sources1
Modified residuei112N6-succinyllysine; alternateCombined sources1
Modified residuei119N6-acetyllysineCombined sources1
Modified residuei146N6-acetyllysine; alternateCombined sources1
Modified residuei146N6-succinyllysine; alternateCombined sources1
Modified residuei195PhosphoserineBy similarity1

Post-translational modificationi

During periods of oxidative stress, Sec-47 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP11352
MaxQBiP11352
PaxDbiP11352
PeptideAtlasiP11352
PRIDEiP11352

2D gel databases

REPRODUCTION-2DPAGEiIPI00319652
P11352
SWISS-2DPAGEiP11352

PTM databases

iPTMnetiP11352
PhosphoSitePlusiP11352
SwissPalmiP11352

Expressioni

Gene expression databases

BgeeiENSMUSG00000063856 Expressed in 304 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_GPX1
ExpressionAtlasiP11352 baseline and differential
GenevisibleiP11352 MM

Interactioni

Subunit structurei

Homotetramer. Interacts with MIEN1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200037, 2 interactors
IntActiP11352, 9 interactors
MINTiP11352
STRINGi10090.ENSMUSP00000081010

Structurei

3D structure databases

ProteinModelPortaliP11352
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Phylogenomic databases

eggNOGiKOG1651 Eukaryota
COG0386 LUCA
GeneTreeiENSGT00760000119230
HOGENOMiHOG000277055
HOVERGENiHBG004333
InParanoidiP11352
KOiK00432
OMAiRDYTEMN
OrthoDBiEOG091G10LN
PhylomeDBiP11352
TreeFamiTF105318

Family and domain databases

CDDicd00340 GSH_Peroxidase, 1 hit
InterProiView protein in InterPro
IPR000889 Glutathione_peroxidase
IPR029759 GPX_AS
IPR029760 GPX_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11592 PTHR11592, 1 hit
PfamiView protein in Pfam
PF00255 GSHPx, 1 hit
PIRSFiPIRSF000303 Glutathion_perox, 1 hit
PRINTSiPR01011 GLUTPROXDASE
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00460 GLUTATHIONE_PEROXID_1, 1 hit
PS00763 GLUTATHIONE_PEROXID_2, 1 hit
PS51355 GLUTATHIONE_PEROXID_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P11352-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCAARLSAAA QSTVYAFSAR PLTGGEPVSL GSLRGKVLLI ENVASLUGTT
60 70 80 90 100
IRDYTEMNDL QKRLGPRGLV VLGFPCNQFG HQENGKNEEI LNSLKYVRPG
110 120 130 140 150
GGFEPNFTLF EKCEVNGEKA HPLFTFLRNA LPTPSDDPTA LMTDPKYIIW
160 170 180 190 200
SPVCRNDIAW NFEKFLVGPD GVPVRRYSRR FRTIDIEPDI ETLLSQQSGN

S
Length:201
Mass (Da):22,329
Last modified:February 26, 2008 - v2
Checksum:i401D065165D8AF5C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YVV2A0A0A6YVV2_MOUSE
Glutathione peroxidase
Gpx1
145Annotation score:
A0A0A6YY34A0A0A6YY34_MOUSE
Glutathione peroxidase
Gpx1
144Annotation score:

Sequence cautioni

The sequence CAB43535 differs from that shown. Number of sequencing artifacts.Curated

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei47Selenocysteine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03920 Genomic DNA Translation: CAA27558.1
AK002245 mRNA Translation: BAC55244.1
AK010999 mRNA Translation: BAC55252.1
AK011019 mRNA Translation: BAC55253.1
AK028171 mRNA Translation: BAC55257.1
AK150548 mRNA Translation: BAE29650.1
AK154833 mRNA Translation: BAE32862.1
AK160388 mRNA Translation: BAE35760.1
BC086649 mRNA Translation: AAH86649.1
X15667 mRNA Translation: CAB43535.1 Sequence problems.
CCDSiCCDS23522.1
PIRiA25106 OPMSE
S05317
RefSeqiNP_001316456.1, NM_001329527.1
NP_001316457.1, NM_001329528.1
NP_032186.2, NM_008160.6
UniGeneiMm.1090

Genome annotation databases

EnsembliENSMUST00000082429; ENSMUSP00000081010; ENSMUSG00000063856
GeneIDi14775
KEGGimmu:14775
UCSCiuc009rpf.3 mouse

Keywords - Coding sequence diversityi

Selenocysteine

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03920 Genomic DNA Translation: CAA27558.1
AK002245 mRNA Translation: BAC55244.1
AK010999 mRNA Translation: BAC55252.1
AK011019 mRNA Translation: BAC55253.1
AK028171 mRNA Translation: BAC55257.1
AK150548 mRNA Translation: BAE29650.1
AK154833 mRNA Translation: BAE32862.1
AK160388 mRNA Translation: BAE35760.1
BC086649 mRNA Translation: AAH86649.1
X15667 mRNA Translation: CAB43535.1 Sequence problems.
CCDSiCCDS23522.1
PIRiA25106 OPMSE
S05317
RefSeqiNP_001316456.1, NM_001329527.1
NP_001316457.1, NM_001329528.1
NP_032186.2, NM_008160.6
UniGeneiMm.1090

3D structure databases

ProteinModelPortaliP11352
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200037, 2 interactors
IntActiP11352, 9 interactors
MINTiP11352
STRINGi10090.ENSMUSP00000081010

Chemistry databases

SwissLipidsiSLP:000001635

Protein family/group databases

PeroxiBasei3709 MmGPx01

PTM databases

iPTMnetiP11352
PhosphoSitePlusiP11352
SwissPalmiP11352

2D gel databases

REPRODUCTION-2DPAGEiIPI00319652
P11352
SWISS-2DPAGEiP11352

Proteomic databases

EPDiP11352
MaxQBiP11352
PaxDbiP11352
PeptideAtlasiP11352
PRIDEiP11352

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082429; ENSMUSP00000081010; ENSMUSG00000063856
GeneIDi14775
KEGGimmu:14775
UCSCiuc009rpf.3 mouse

Organism-specific databases

CTDi2876
MGIiMGI:104887 Gpx1

Phylogenomic databases

eggNOGiKOG1651 Eukaryota
COG0386 LUCA
GeneTreeiENSGT00760000119230
HOGENOMiHOG000277055
HOVERGENiHBG004333
InParanoidiP11352
KOiK00432
OMAiRDYTEMN
OrthoDBiEOG091G10LN
PhylomeDBiP11352
TreeFamiTF105318

Enzyme and pathway databases

ReactomeiR-MMU-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-MMU-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-74259 Purine catabolism
SABIO-RKiP11352

Miscellaneous databases

ChiTaRSiGpx1 mouse
PROiPR:P11352
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063856 Expressed in 304 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_GPX1
ExpressionAtlasiP11352 baseline and differential
GenevisibleiP11352 MM

Family and domain databases

CDDicd00340 GSH_Peroxidase, 1 hit
InterProiView protein in InterPro
IPR000889 Glutathione_peroxidase
IPR029759 GPX_AS
IPR029760 GPX_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11592 PTHR11592, 1 hit
PfamiView protein in Pfam
PF00255 GSHPx, 1 hit
PIRSFiPIRSF000303 Glutathion_perox, 1 hit
PRINTSiPR01011 GLUTPROXDASE
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00460 GLUTATHIONE_PEROXID_1, 1 hit
PS00763 GLUTATHIONE_PEROXID_2, 1 hit
PS51355 GLUTATHIONE_PEROXID_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGPX1_MOUSE
AccessioniPrimary (citable) accession number: P11352
Secondary accession number(s): P12079
, Q544W3, Q5RJH8, Q9CR54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 26, 2008
Last modified: November 7, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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