UniProtKB - P11344 (TYRO_MOUSE)
Tyrosinase
Tyr
Functioni
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (PubMed:2517217).
Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (PubMed:2517217, PubMed:1537333).
In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:1537333).
2 PublicationsCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 180 | Copper ABy similarity | 1 | |
Metal bindingi | 202 | Copper ABy similarity | 1 | |
Metal bindingi | 211 | Copper ABy similarity | 1 | |
Metal bindingi | 363 | Copper BBy similarity | 1 | |
Metal bindingi | 367 | Copper BBy similarity | 1 | |
Metal bindingi | 390 | Copper BBy similarity | 1 |
GO - Molecular functioni
- copper ion binding Source: MGI
- identical protein binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- tyrosinase activity Source: MGI
GO - Biological processi
- cell population proliferation Source: MGI
- melanin biosynthetic process Source: MGI
- pigmentation Source: MGI
- response to blue light Source: UniProtKB
- response to cAMP Source: Ensembl
- response to UV Source: Ensembl
- response to vitamin D Source: Ensembl
- thymus development Source: MGI
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Melanin biosynthesis |
Ligand | Copper, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.14.18.1, 3474 |
Reactomei | R-MMU-5662702, Melanin biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosinase (EC:1.14.18.1)Alternative name(s): Albino locus protein Monophenol monooxygenase |
Gene namesi | Name:Tyr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98880, Tyr |
VEuPathDBi | HostDB:ENSMUSG00000004651 |
Subcellular locationi
Other locations
- Melanosome membrane By similarity; Single-pass type I membrane protein By similarity
- Melanosome 1 Publication
Note: Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB
- intracellular membrane-bounded organelle Source: MGI
- melanosome Source: UniProtKB
- melanosome membrane Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 476 | Lumenal, melanosomeSequence analysisAdd BLAST | 458 | |
Transmembranei | 477 – 497 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 498 – 533 | CytoplasmicSequence analysisAdd BLAST | 36 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Involvement in diseasei
Keywords - Diseasei
Albinism, Disease variantChemistry databases
ChEMBLi | CHEMBL5346 |
DrugCentrali | P11344 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000035880 | 19 – 533 | TyrosinaseAdd BLAST | 515 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 86 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 111 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 161 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 230 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 371 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P11344 |
PRIDEi | P11344 |
ProteomicsDBi | 298079 |
PTM databases
GlyGeni | P11344, 6 sites |
PhosphoSitePlusi | P11344 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000004651, Expressed in iris and 92 other tissues |
Genevisiblei | P11344, MM |
Interactioni
Subunit structurei
Forms an OPN3-dependent complex with DCT in response to blue light in melanocytes.
By similarityBinary interactionsi
P11344
With | #Exp. | IntAct |
---|---|---|
Tyrp1 [P07147] | 8 | EBI-821603,EBI-821614 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204394, 8 interactors |
ELMi | P11344 |
IntActi | P11344, 2 interactors |
STRINGi | 10090.ENSMUSP00000004770 |
Chemistry databases
BindingDBi | P11344 |
Miscellaneous databases
RNActi | P11344, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QRET, Eukaryota |
GeneTreei | ENSGT00940000155336 |
HOGENOMi | CLU_038693_1_0_1 |
InParanoidi | P11344 |
OMAi | HWAPAFS |
OrthoDBi | 881347at2759 |
PhylomeDBi | P11344 |
TreeFami | TF315865 |
Family and domain databases
Gene3Di | 1.10.1280.10, 1 hit |
InterProi | View protein in InterPro IPR008922, Di-copper_centre_dom_sf IPR002227, Tyrosinase_Cu-bd |
Pfami | View protein in Pfam PF00264, Tyrosinase, 1 hit |
PRINTSi | PR00092, TYROSINASE |
SUPFAMi | SSF48056, SSF48056, 1 hit |
PROSITEi | View protein in PROSITE PS00497, TYROSINASE_1, 1 hit PS00498, TYROSINASE_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFLAVLYCLL WSFQISDGHF PRACASSKNL LAKECCPPWM GDGSPCGQLS
60 70 80 90 100
GRGSCQDILL SSAPSGPQFP FKGVDDRESW PSVFYNRTCQ CSGNFMGFNC
110 120 130 140 150
GNCKFGFGGP NCTEKRVLIR RNIFDLSVSE KNKFFSYLTL AKHTISSVYV
160 170 180 190 200
IPTGTYGQMN NGSTPMFNDI NIYDLFVWMH YYVSRDTLLG GSEIWRDIDF
210 220 230 240 250
AHEAPGFLPW HRLFLLLWEQ EIRELTGDEN FTVPYWDWRD AENCDICTDE
260 270 280 290 300
YLGGRHPENP NLLSPASFFS SWQIICSRSE EYNSHQVLCD GTPEGPLLRN
310 320 330 340 350
PGNHDKAKTP RLPSSADVEF CLSLTQYESG SMDRTANFSF RNTLEGFASP
360 370 380 390 400
LTGIADPSQS SMHNALHIFM NGTMSQVQGS ANDPIFLLHH AFVDSIFEQW
410 420 430 440 450
LRRHRPLLEV YPEANAPIGH NRDSYMVPFI PLYRNGDFFI TSKDLGYDYS
460 470 480 490 500
YLQESDPGFY RNYIEPYLEQ ASRIWPWLLG AALVGAVIAA ALSGLSSRLC
510 520 530
LQKKKKKKQP QEERQPLLMD KDDYHSLLYQ SHL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIB9 | A0A140LIB9_MOUSE | Tyrosinase | Tyr | 158 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | M → I in AAA37806 (PubMed:2567165).Curated | 1 | |
Sequence conflicti | 197 | D → Q in AAA37806 (PubMed:2567165).Curated | 1 | |
Sequence conflicti | 264 | S → I in CAA31273 (PubMed:3141148).Curated | 1 | |
Sequence conflicti | 346 | G → V in AAA40516 (PubMed:3134020).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 103 | C → S in albino mice. 3 Publications | 1 | |
Natural varianti | 420 | H → R in strain: Himalayan. 1 Publication | 1 | |
Natural varianti | 482 | A → T in chinchilla mice. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00440 mRNA Translation: BAA00341.1 D00131 mRNA Translation: BAA00079.1 Sequence problems. M20234 mRNA Translation: AAA40516.1 X12782 mRNA Translation: CAA31273.1 M26729 mRNA Translation: AAA37806.1 M24560 mRNA Translation: AAA40517.1 D00439 Genomic DNA Translation: BAA00340.1 X51743 Genomic DNA Translation: CAA36033.1 |
CCDSi | CCDS52304.1 |
PIRi | A27711, YRMSCS |
RefSeqi | NP_035791.1, NM_011661.5 |
Genome annotation databases
Ensembli | ENSMUST00000004770; ENSMUSP00000004770; ENSMUSG00000004651 |
GeneIDi | 22173 |
KEGGi | mmu:22173 |
UCSCi | uc009ifo.1, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Snowy stardom - Issue 49 of August 2004 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00440 mRNA Translation: BAA00341.1 D00131 mRNA Translation: BAA00079.1 Sequence problems. M20234 mRNA Translation: AAA40516.1 X12782 mRNA Translation: CAA31273.1 M26729 mRNA Translation: AAA37806.1 M24560 mRNA Translation: AAA40517.1 D00439 Genomic DNA Translation: BAA00340.1 X51743 Genomic DNA Translation: CAA36033.1 |
CCDSi | CCDS52304.1 |
PIRi | A27711, YRMSCS |
RefSeqi | NP_035791.1, NM_011661.5 |
3D structure databases
AlphaFoldDBi | P11344 |
SMRi | P11344 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204394, 8 interactors |
ELMi | P11344 |
IntActi | P11344, 2 interactors |
STRINGi | 10090.ENSMUSP00000004770 |
Chemistry databases
BindingDBi | P11344 |
ChEMBLi | CHEMBL5346 |
DrugCentrali | P11344 |
PTM databases
GlyGeni | P11344, 6 sites |
PhosphoSitePlusi | P11344 |
Proteomic databases
PaxDbi | P11344 |
PRIDEi | P11344 |
ProteomicsDBi | 298079 |
Protocols and materials databases
Antibodypediai | 3663, 863 antibodies from 37 providers |
DNASUi | 22173 |
Genome annotation databases
Ensembli | ENSMUST00000004770; ENSMUSP00000004770; ENSMUSG00000004651 |
GeneIDi | 22173 |
KEGGi | mmu:22173 |
UCSCi | uc009ifo.1, mouse |
Organism-specific databases
CTDi | 7299 |
MGIi | MGI:98880, Tyr |
VEuPathDBi | HostDB:ENSMUSG00000004651 |
Phylogenomic databases
eggNOGi | ENOG502QRET, Eukaryota |
GeneTreei | ENSGT00940000155336 |
HOGENOMi | CLU_038693_1_0_1 |
InParanoidi | P11344 |
OMAi | HWAPAFS |
OrthoDBi | 881347at2759 |
PhylomeDBi | P11344 |
TreeFami | TF315865 |
Enzyme and pathway databases
BRENDAi | 1.14.18.1, 3474 |
Reactomei | R-MMU-5662702, Melanin biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 22173, 3 hits in 72 CRISPR screens |
ChiTaRSi | Tyr, mouse |
PROi | PR:P11344 |
RNActi | P11344, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004651, Expressed in iris and 92 other tissues |
Genevisiblei | P11344, MM |
Family and domain databases
Gene3Di | 1.10.1280.10, 1 hit |
InterProi | View protein in InterPro IPR008922, Di-copper_centre_dom_sf IPR002227, Tyrosinase_Cu-bd |
Pfami | View protein in Pfam PF00264, Tyrosinase, 1 hit |
PRINTSi | PR00092, TYROSINASE |
SUPFAMi | SSF48056, SSF48056, 1 hit |
PROSITEi | View protein in PROSITE PS00497, TYROSINASE_1, 1 hit PS00498, TYROSINASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TYRO_MOUSE | |
Accessioni | P11344Primary (citable) accession number: P11344 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | May 29, 2007 | |
Last modified: | May 25, 2022 | |
This is version 198 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries