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Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

PubMed:3034607 authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene.
Present with 2120 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei649ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • leucine-tRNA ligase activity Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • Group I intron splicing Source: SGD
  • leucyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32448-MONOMER

Protein family/group databases

MoonProtiP11325

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:NAM2
Synonyms:MSL1
Ordered Locus Names:YLR382C
ORF Names:L3502.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR382C
SGDiS000004374 NAM2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 9Mitochondrion9
ChainiPRO_000003581010 – 894Leucine--tRNA ligase, mitochondrialAdd BLAST885

Proteomic databases

MaxQBiP11325
PaxDbiP11325
PRIDEiP11325

PTM databases

CarbonylDBiP11325

Interactioni

Protein-protein interaction databases

BioGridi31641, 60 interactors
ComplexPortaliCPX-1314 bI4 intron splicing factor complex
IntActiP11325, 9 interactors
MINTiP11325
STRINGi4932.YLR382C

Structurei

3D structure databases

ProteinModelPortaliP11325
SMRiP11325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi56 – 66"HIGH" regionAdd BLAST11
Motifi646 – 650"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000015114
HOGENOMiHOG000200747
InParanoidiP11325
KOiK01869
OMAiTFMVLAP
OrthoDBiEOG092C119T

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 1 hit
PfamiView protein in Pfam
PF00133 tRNA-synt_1, 1 hit
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 2 hits
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY
60 70 80 90 100
ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA
110 120 130 140 150
ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT
160 170 180 190 200
QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK
210 220 230 240 250
KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK
260 270 280 290 300
VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK
310 320 330 340 350
SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC
360 370 380 390 400
PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS
410 420 430 440 450
TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ
460 470 480 490 500
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN
510 520 530 540 550
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP
560 570 580 590 600
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV
610 620 630 640 650
QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS
660 670 680 690 700
KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV
710 720 730 740 750
LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV
760 770 780 790 800
NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI
810 820 830 840 850
SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN
860 870 880 890
FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK
Length:894
Mass (Da):101,920
Last modified:July 1, 1989 - v1
Checksum:i3862CEFFFB10B262
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03495 Genomic DNA Translation: AAA34805.1
X05143 Genomic DNA Translation: CAA28791.1
U19104 Genomic DNA Translation: AAB67277.1
BK006945 Genomic DNA Translation: DAA09683.1
PIRiA28521 SYBYLM
RefSeqiNP_013486.3, NM_001182271.3

Genome annotation databases

EnsemblFungiiYLR382C; YLR382C; YLR382C
GeneIDi851098
KEGGisce:YLR382C

Similar proteinsi

Entry informationi

Entry nameiSYLM_YEAST
AccessioniPrimary (citable) accession number: P11325
Secondary accession number(s): D6VZ17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 18, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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