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Entry version 182 (29 Sep 2021)
Sequence version 1 (01 Jul 1989)
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Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

PubMed:3034607 authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene.
Present with 2120 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei649ATPBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • leucine-tRNA ligase activity Source: SGD
  • pre-mRNA intronic binding Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Protein family/group databases

MoonProt database of moonlighting proteins

More...
MoonProti
P11325

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NAM2
Synonyms:MSL1
Ordered Locus Names:YLR382C
ORF Names:L3502.6
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000004374, NAM2

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YLR382C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 9Mitochondrion9
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003581010 – 894Leucine--tRNA ligase, mitochondrialAdd BLAST885

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P11325

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P11325

PRoteomics IDEntifications database

More...
PRIDEi
P11325

PTM databases

CarbonylDB database of protein carbonylation sites

More...
CarbonylDBi
P11325

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31641, 61 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1314, bI4 intron splicing factor complex

Protein interaction database and analysis system

More...
IntActi
P11325, 2 interactors

Molecular INTeraction database

More...
MINTi
P11325

STRING: functional protein association networks

More...
STRINGi
4932.YLR382C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P11325, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P11325

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi56 – 66'HIGH' regionAdd BLAST11
Motifi646 – 650'KMSKS' region5

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0435, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000015114

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004427_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P11325

Identification of Orthologs from Complete Genome Data

More...
OMAi
YANIETQ

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.620, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002300, aa-tRNA-synth_Ia
IPR002302, Leu-tRNA-ligase
IPR025709, Leu_tRNA-synth_edit
IPR015413, Methionyl/Leucyl_tRNA_Synth
IPR014729, Rossmann-like_a/b/a_fold
IPR009080, tRNAsynth_Ia_anticodon-bd
IPR009008, Val/Leu/Ile-tRNA-synth_edit

The PANTHER Classification System

More...
PANTHERi
PTHR43740, PTHR43740, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00133, tRNA-synt_1, 1 hit
PF13603, tRNA-synt_1_2, 1 hit
PF09334, tRNA-synt_1g, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00985, TRNASYNTHLEU

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47323, SSF47323, 1 hit
SSF50677, SSF50677, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00396, leuS_bact, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P11325-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY
60 70 80 90 100
ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA
110 120 130 140 150
ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT
160 170 180 190 200
QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK
210 220 230 240 250
KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK
260 270 280 290 300
VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK
310 320 330 340 350
SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC
360 370 380 390 400
PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS
410 420 430 440 450
TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ
460 470 480 490 500
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN
510 520 530 540 550
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP
560 570 580 590 600
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV
610 620 630 640 650
QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS
660 670 680 690 700
KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV
710 720 730 740 750
LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV
760 770 780 790 800
NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI
810 820 830 840 850
SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN
860 870 880 890
FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK
Length:894
Mass (Da):101,920
Last modified:July 1, 1989 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3862CEFFFB10B262
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
J03495 Genomic DNA Translation: AAA34805.1
X05143 Genomic DNA Translation: CAA28791.1
U19104 Genomic DNA Translation: AAB67277.1
BK006945 Genomic DNA Translation: DAA09683.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A28521, SYBYLM

NCBI Reference Sequences

More...
RefSeqi
NP_013486.3, NM_001182271.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLR382C_mRNA; YLR382C; YLR382C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
851098

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLR382C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03495 Genomic DNA Translation: AAA34805.1
X05143 Genomic DNA Translation: CAA28791.1
U19104 Genomic DNA Translation: AAB67277.1
BK006945 Genomic DNA Translation: DAA09683.1
PIRiA28521, SYBYLM
RefSeqiNP_013486.3, NM_001182271.3

3D structure databases

SMRiP11325
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi31641, 61 interactors
ComplexPortaliCPX-1314, bI4 intron splicing factor complex
IntActiP11325, 2 interactors
MINTiP11325
STRINGi4932.YLR382C

Protein family/group databases

MoonProtiP11325

PTM databases

CarbonylDBiP11325

Proteomic databases

MaxQBiP11325
PaxDbiP11325
PRIDEiP11325

Genome annotation databases

EnsemblFungiiYLR382C_mRNA; YLR382C; YLR382C
GeneIDi851098
KEGGisce:YLR382C

Organism-specific databases

SGDiS000004374, NAM2
VEuPathDBiFungiDB:YLR382C

Phylogenomic databases

eggNOGiKOG0435, Eukaryota
GeneTreeiENSGT00390000015114
HOGENOMiCLU_004427_2_0_1
InParanoidiP11325
OMAiYANIETQ

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P11325
RNActiP11325, protein

Family and domain databases

Gene3Di3.40.50.620, 2 hits
InterProiView protein in InterPro
IPR001412, aa-tRNA-synth_I_CS
IPR002300, aa-tRNA-synth_Ia
IPR002302, Leu-tRNA-ligase
IPR025709, Leu_tRNA-synth_edit
IPR015413, Methionyl/Leucyl_tRNA_Synth
IPR014729, Rossmann-like_a/b/a_fold
IPR009080, tRNAsynth_Ia_anticodon-bd
IPR009008, Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740, PTHR43740, 1 hit
PfamiView protein in Pfam
PF00133, tRNA-synt_1, 1 hit
PF13603, tRNA-synt_1_2, 1 hit
PF09334, tRNA-synt_1g, 1 hit
PRINTSiPR00985, TRNASYNTHLEU
SUPFAMiSSF47323, SSF47323, 1 hit
SSF50677, SSF50677, 1 hit
TIGRFAMsiTIGR00396, leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178, AA_TRNA_LIGASE_I, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYLM_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P11325
Secondary accession number(s): D6VZ17
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 29, 2021
This is version 182 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  4. SIMILARITY comments
    Index of protein domains and families
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