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UniProtKB - P11258 (A27_VACCW)
Protein
Protein A27
Gene
VACWR150
Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Functioni
Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.
2 PublicationsGO - Biological processi
- fusion of virus membrane with host plasma membrane Source: InterPro
Keywordsi
Biological process | Host-virus interaction |
Names & Taxonomyi
Protein namesi | Recommended name: Protein A27 |
Gene namesi | Ordered Locus Names:VACWR150 ORF Names:A27L |
Organismi | Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) |
Taxonomic identifieri | 10254 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Nucleocytoviricota › Pokkesviricetes › Chitovirales › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Proteomesi |
|
Subcellular locationi
- Virion Note: Located to the mature virion membrane via interaction with protein A17.
Keywords - Cellular componenti
VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | L → A: Forms only tetramers, no hexamer. 1 Publication | 1 | |
Mutagenesisi | 54 | L → A: Forms more tetramers than hexamers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000099212 | 1 – 110 | Protein A27Add BLAST | 110 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 71 | Interchain (with C-441 in A26)1 Publication | ||
Disulfide bondi | 72 | Interchain (with C-442 in A26)1 Publication |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Homohexamers, covalently linked.
Interacts with A17 and A26.
2 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P11258 |
SMRi | P11258 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 36 – 101 | Sequence analysisAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the chordopoxvirinae A27 protein family.Curated
Keywords - Domaini
Coiled coilFamily and domain databases
InterProi | View protein in InterPro IPR003436, Chordopox_Fusion/A27 |
Pfami | View protein in Pfam PF02346, Vac_Fusion, 1 hit |
PRINTSi | PR01847, VIRALFUSION |
i Sequence
Sequence statusi: Complete.
P11258-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDGTLFPGDD DLAIPATEFF STKAAKKPEA KREAIVKADE DDNEETLKQR
60 70 80 90 100
LTNLEKKITN VTTKFEQIEK CCKRNDEVLF RLENHAETLR AAMISLAKKI
110
DVQTGRRPYE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 – 30 | EA → DR in AAA48324 (PubMed:1856205).Curated | 2 | |
Sequence conflicti | 29 – 30 | EA → DR in CAA40577 (Ref. 3) Curated | 2 | |
Sequence conflicti | 34 | A → Q in AAA48324 (PubMed:1856205).Curated | 1 | |
Sequence conflicti | 34 | A → Q in CAA40577 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61187 Genomic DNA Translation: AAA48324.1 M18173 Genomic DNA Translation: AAA48248.1 X57318 Genomic DNA Translation: CAA40577.1 AY243312 Genomic DNA Translation: AAO89429.1 |
PIRi | A27173, WMVZ14 |
RefSeqi | YP_233032.1, NC_006998.1 |
Genome annotation databases
GeneIDi | 3707680 |
KEGGi | vg:3707680 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61187 Genomic DNA Translation: AAA48324.1 M18173 Genomic DNA Translation: AAA48248.1 X57318 Genomic DNA Translation: CAA40577.1 AY243312 Genomic DNA Translation: AAO89429.1 |
PIRi | A27173, WMVZ14 |
RefSeqi | YP_233032.1, NC_006998.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VOP | X-ray | 2.20 | A/B/C | 21-84 | [»] | |
5EOQ | X-ray | 1.95 | A | 31-40 | [»] | |
5EOR | X-ray | 2.27 | A | 101-110 | [»] | |
BMRBi | P11258 | |||||
SMRi | P11258 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protocols and materials databases
ABCDi | P11258, 5 sequenced antibodies |
DNASUi | 3707680 |
Genome annotation databases
GeneIDi | 3707680 |
KEGGi | vg:3707680 |
Family and domain databases
InterProi | View protein in InterPro IPR003436, Chordopox_Fusion/A27 |
Pfami | View protein in Pfam PF02346, Vac_Fusion, 1 hit |
PRINTSi | PR01847, VIRALFUSION |
MobiDBi | Search... |
Entry informationi
Entry namei | A27_VACCW | |
Accessioni | P11258Primary (citable) accession number: P11258 Secondary accession number(s): Q76ZP6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 93 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families