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Protein

Arylamine N-acetyltransferase 2

Gene

NAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.

Catalytic activityi

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Acyl-thioester intermediate1
Binding sitei103Coenzyme A1 Publication1
Binding sitei104Coenzyme A; via amide nitrogen1 Publication1
Active sitei1071
Active sitei1221
Binding sitei208Coenzyme A1 Publication1
Binding sitei214Coenzyme A1 Publication1
Binding sitei287Coenzyme A1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.5 2681
ReactomeiR-HSA-156582 Acetylation

Names & Taxonomyi

Protein namesi
Recommended name:
Arylamine N-acetyltransferase 2 (EC:2.3.1.5)
Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name:
NAT-2
Polymorphic arylamine N-acetyltransferase
Short name:
PNAT
Gene namesi
Name:NAT2
Synonyms:AAC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000156006.4
HGNCiHGNC:7646 NAT2
MIMi612182 gene
neXtProtiNX_P11245

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi10
MalaCardsiNAT2
MIMi243400 phenotype
Orphaneti240887 Isoniazid toxicity
PharmGKBiPA18

Chemistry databases

ChEMBLiCHEMBL2194
DrugBankiDB00316 Acetaminophen
DB01068 Clonazepam
DB00250 Dapsone
DB04953 Ezogabine
DB00951 Isoniazid
DB01015 Sulfamethoxazole

Polymorphism and mutation databases

BioMutaiNAT2
DMDMi114238

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001079051 – 290Arylamine N-acetyltransferase 2Add BLAST290

Proteomic databases

MaxQBiP11245
PaxDbiP11245
PeptideAtlasiP11245
PRIDEiP11245
ProteomicsDBi52728

PTM databases

iPTMnetiP11245
PhosphoSitePlusiP11245

Expressioni

Gene expression databases

BgeeiENSG00000156006
CleanExiHS_NAT2
ExpressionAtlasiP11245 baseline and differential
GenevisibleiP11245 HS

Organism-specific databases

HPAiHPA043704

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi106528, 20 interactors
IntActiP11245, 19 interactors
MINTiP11245
STRINGi9606.ENSP00000286479

Structurei

Secondary structure

1290
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 9Combined sources8
Helixi21 – 34Combined sources14
Helixi40 – 43Combined sources4
Helixi52 – 59Combined sources8
Helixi68 – 82Combined sources15
Beta strandi85 – 95Combined sources11
Helixi96 – 98Combined sources3
Beta strandi107 – 114Combined sources8
Beta strandi117 – 121Combined sources5
Helixi127 – 129Combined sources3
Beta strandi142 – 144Combined sources3
Beta strandi149 – 155Combined sources7
Beta strandi158 – 164Combined sources7
Beta strandi167 – 171Combined sources5
Helixi173 – 175Combined sources3
Beta strandi185 – 192Combined sources8
Helixi199 – 202Combined sources4
Helixi203 – 211Combined sources9
Helixi216 – 219Combined sources4
Beta strandi222 – 226Combined sources5
Beta strandi228 – 235Combined sources8
Beta strandi238 – 247Combined sources10
Beta strandi250 – 257Combined sources8
Helixi260 – 269Combined sources10
Beta strandi285 – 288Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PFRX-ray1.92A/B2-290[»]
ProteinModelPortaliP11245
SMRiP11245
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11245

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 107Substrate bindingCurated2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFD6 Eukaryota
COG2162 LUCA
HOVERGENiHBG000165
InParanoidiP11245
KOiK00622
OrthoDBiEOG091G0CO6
PhylomeDBiP11245
TreeFamiTF106311

Family and domain databases

InterProiView protein in InterPro
IPR001447 Arylamine_N-AcTrfase
IPR038765 Papain_like_cys_pep_sf
PANTHERiPTHR11786 PTHR11786, 1 hit
PfamiView protein in Pfam
PF00797 Acetyltransf_2, 1 hit
PRINTSiPR01543 ANATRNSFRASE
SUPFAMiSSF54001 SSF54001, 1 hit

Sequencei

Sequence statusi: Complete.

P11245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEAYFERI GYKNSRNKLD LETLTDILEH QIRAVPFENL NMHCGQAMEL
60 70 80 90 100
GLEAIFDHIV RRNRGGWCLQ VNQLLYWALT TIGFQTTMLG GYFYIPPVNK
110 120 130 140 150
YSTGMVHLLL QVTIDGRNYI VDAGSGSSSQ MWQPLELISG KDQPQVPCIF
160 170 180 190 200
CLTEERGIWY LDQIRREQYI TNKEFLNSHL LPKKKHQKIY LFTLEPRTIE
210 220 230 240 250
DFESMNTYLQ TSPTSSFITT SFCSLQTPEG VYCLVGFILT YRKFNYKDNT
260 270 280 290
DLVEFKTLTE EEVEEVLKNI FKISLGRNLV PKPGDGSLTI
Length:290
Mass (Da):33,542
Last modified:July 1, 1989 - v1
Checksum:i63EE3BC2107F12B9
GO

Sequence cautioni

The sequence AAK51711 differs from that shown. Reason: Frameshift at position 287.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6Y → D in CAG28559 (Ref. 13) Curated1
Sequence conflicti30H → T AA sequence (PubMed:2924904).Curated1
Sequence conflicti286G → R in AAG34181 (Ref. 9) Curated1

Polymorphismi

Polymorphisms in NAT2 are the cause of slow and fast acetylation phenotypes [MIMi:243400] and influence drug therapy response and susceptibility to chemical toxicity or carcinogenicity.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01885324L → I2 PublicationsCorresponds to variant dbSNP:rs45477599Ensembl.1
Natural variantiVAR_00460864R → Q in allele NAT2*14A, allele NAT2*14B, allele NAT2*14C, allele NAT2*14D, allele NAT2*14E, allele NAT2*14F and allele NAT2*14G; a slow acetylator. 4 PublicationsCorresponds to variant dbSNP:rs1801279EnsemblClinVar.1
Natural variantiVAR_00907564R → W in allele NAT2*19. Corresponds to variant dbSNP:rs1805158Ensembl.1
Natural variantiVAR_004609114I → T in allele NAT2*5A, allele NAT2*5B, allele NAT2*5C, allele NAT2*5D, allele NAT2*5E, allele NAT2*5F, allele NAT2*14B and allele NAT2*14E; a slow acetylator. 8 PublicationsCorresponds to variant dbSNP:rs1801280EnsemblClinVar.1
Natural variantiVAR_020385122D → N1 PublicationCorresponds to variant dbSNP:rs4986996Ensembl.1
Natural variantiVAR_046905135L → V. Corresponds to variant dbSNP:rs12720065Ensembl.1
Natural variantiVAR_020386137L → F1 PublicationCorresponds to variant dbSNP:rs4986997Ensembl.1
Natural variantiVAR_009076145Q → P in allele NAT2*17. 1 Publication1
Natural variantiVAR_028781193T → M1 Publication1
Natural variantiVAR_004610197R → Q in allele NAT2*5E, allele NAT2*6A, allele NAT2*6B, allele NAT2*6C, allele NAT2*6D and allele NAT2*14D; a slow acetylator. 5 PublicationsCorresponds to variant dbSNP:rs1799930EnsemblClinVar.1
Natural variantiVAR_028782208Y → H1 PublicationCorresponds to variant dbSNP:rs56387565Ensembl.1
Natural variantiVAR_018854228P → L1 PublicationCorresponds to variant dbSNP:rs45518335Ensembl.1
Natural variantiVAR_004611268K → R in allele NAT2*5B, allele NAT2*5C, allele NAT2*5F, allele NAT2*6C, allele NAT2*12A, allele NAT2*14B, allele NAT2*14C, allele NAT2*14E, allele NAT2*14F and allele NAT2*14G. 8 PublicationsCorresponds to variant dbSNP:rs1208Ensembl.1
Natural variantiVAR_061368280V → M. Corresponds to variant dbSNP:rs56393504Ensembl.1
Natural variantiVAR_009077282K → T in allele NAT2*18. 1 PublicationCorresponds to variant dbSNP:rs56054745Ensembl.1
Natural variantiVAR_004612286G → E in allele NAT2*7A and allele NAT2*7B; a slow acetylator. 4 PublicationsCorresponds to variant dbSNP:rs1799931EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14672 Genomic DNA Translation: CAA32802.1
D90040 mRNA Translation: BAA14094.1
D90042 mRNA Translation: BAA14096.1
D10870 Genomic DNA Translation: BAA01640.1
D10871 Genomic DNA Translation: BAA01641.1
D10872 Genomic DNA Translation: BAA01642.1
U23052 Genomic DNA Translation: AAA64584.1
U23434 Genomic DNA Translation: AAA64585.1
DQ305496 Genomic DNA Translation: ABC26032.1
DQ305497 Genomic DNA Translation: ABC26033.1
DQ305498 Genomic DNA Translation: ABC26034.1
DQ305499 Genomic DNA Translation: ABC26035.1
DQ305500 Genomic DNA Translation: ABC26036.1
DQ305501 Genomic DNA Translation: ABC26037.1
DQ305502 Genomic DNA Translation: ABC26038.1
DQ305503 Genomic DNA Translation: ABC26039.1
DQ305504 Genomic DNA Translation: ABC26040.1
DQ305505 Genomic DNA Translation: ABC26041.1
DQ305506 Genomic DNA Translation: ABC26042.1
DQ305507 Genomic DNA Translation: ABC26043.1
DQ305508 Genomic DNA Translation: ABC26044.1
DQ305509 Genomic DNA Translation: ABC26045.1
DQ305510 Genomic DNA Translation: ABC26046.1
DQ305511 Genomic DNA Translation: ABC26047.1
DQ305512 Genomic DNA Translation: ABC26048.1
DQ305513 Genomic DNA Translation: ABC26049.1
DQ305514 Genomic DNA Translation: ABC26050.1
DQ305515 Genomic DNA Translation: ABC26051.1
DQ305516 Genomic DNA Translation: ABC26052.1
DQ305517 Genomic DNA Translation: ABC26053.1
DQ305518 Genomic DNA Translation: ABC26054.1
DQ305519 Genomic DNA Translation: ABC26055.1
DQ305520 Genomic DNA Translation: ABC26056.1
DQ305521 Genomic DNA Translation: ABC26057.1
DQ305522 Genomic DNA Translation: ABC26058.1
DQ305523 Genomic DNA Translation: ABC26059.1
DQ305524 Genomic DNA Translation: ABC26060.1
DQ305525 Genomic DNA Translation: ABC26061.1
DQ305526 Genomic DNA Translation: ABC26062.1
DQ305527 Genomic DNA Translation: ABC26063.1
DQ305528 Genomic DNA Translation: ABC26064.1
DQ305529 Genomic DNA Translation: ABC26065.1
DQ305530 Genomic DNA Translation: ABC26066.1
DQ305531 Genomic DNA Translation: ABC26067.1
DQ305532 Genomic DNA Translation: ABC26068.1
DQ305533 Genomic DNA Translation: ABC26069.1
DQ305534 Genomic DNA Translation: ABC26070.1
DQ305535 Genomic DNA Translation: ABC26071.1
DQ305536 Genomic DNA Translation: ABC26072.1
DQ305537 Genomic DNA Translation: ABC26073.1
DQ305538 Genomic DNA Translation: ABC26074.1
DQ305539 Genomic DNA Translation: ABC26075.1
DQ305540 Genomic DNA Translation: ABC26076.1
DQ305541 Genomic DNA Translation: ABC26077.1
DQ305542 Genomic DNA Translation: ABC26078.1
DQ305543 Genomic DNA Translation: ABC26079.1
DQ305544 Genomic DNA Translation: ABC26080.1
DQ305545 Genomic DNA Translation: ABC26081.1
DQ305546 Genomic DNA Translation: ABC26082.1
DQ305547 Genomic DNA Translation: ABC26083.1
DQ305548 Genomic DNA Translation: ABC26084.1
DQ305549 Genomic DNA Translation: ABC26085.1
DQ305550 Genomic DNA Translation: ABC26086.1
DQ305551 Genomic DNA Translation: ABC26087.1
DQ305552 Genomic DNA Translation: ABC26088.1
DQ305553 Genomic DNA Translation: ABC26089.1
DQ305554 Genomic DNA Translation: ABC26090.1
DQ305555 Genomic DNA Translation: ABC26091.1
DQ305556 Genomic DNA Translation: ABC26092.1
DQ305557 Genomic DNA Translation: ABC26093.1
DQ305558 Genomic DNA Translation: ABC26094.1
DQ305559 Genomic DNA Translation: ABC26095.1
DQ305560 Genomic DNA Translation: ABC26096.1
DQ305561 Genomic DNA Translation: ABC26097.1
DQ305562 Genomic DNA Translation: ABC26098.1
DQ305563 Genomic DNA Translation: ABC26099.1
DQ305564 Genomic DNA Translation: ABC26100.1
DQ305565 Genomic DNA Translation: ABC26101.1
DQ305566 Genomic DNA Translation: ABC26102.1
DQ305567 Genomic DNA Translation: ABC26103.1
DQ305568 Genomic DNA Translation: ABC26104.1
DQ305569 Genomic DNA Translation: ABC26105.1
DQ305570 Genomic DNA Translation: ABC26106.1
DQ305571 Genomic DNA Translation: ABC26107.1
DQ305572 Genomic DNA Translation: ABC26108.1
DQ305573 Genomic DNA Translation: ABC26109.1
DQ305574 Genomic DNA Translation: ABC26110.1
DQ305575 Genomic DNA Translation: ABC26111.1
DQ305576 Genomic DNA Translation: ABC26112.1
DQ305577 Genomic DNA Translation: ABC26113.1
DQ305578 Genomic DNA Translation: ABC26114.1
DQ305579 Genomic DNA Translation: ABC26115.1
DQ305580 Genomic DNA Translation: ABC26116.1
DQ305581 Genomic DNA Translation: ABC26117.1
DQ305582 Genomic DNA Translation: ABC26118.1
DQ305583 Genomic DNA Translation: ABC26119.1
DQ305584 Genomic DNA Translation: ABC26120.1
DQ305585 Genomic DNA Translation: ABC26121.1
DQ305586 Genomic DNA Translation: ABC26122.1
DQ305587 Genomic DNA Translation: ABC26123.1
DQ305588 Genomic DNA Translation: ABC26124.1
DQ305589 Genomic DNA Translation: ABC26125.1
DQ305590 Genomic DNA Translation: ABC26126.1
DQ305591 Genomic DNA Translation: ABC26127.1
DQ305592 Genomic DNA Translation: ABC26128.1
DQ305593 Genomic DNA Translation: ABC26129.1
DQ305594 Genomic DNA Translation: ABC26130.1
DQ305595 Genomic DNA Translation: ABC26131.1
DQ305596 Genomic DNA Translation: ABC26132.1
DQ305597 Genomic DNA Translation: ABC26133.1
DQ305598 Genomic DNA Translation: ABC26134.1
DQ305599 Genomic DNA Translation: ABC26135.1
DQ305600 Genomic DNA Translation: ABC26136.1
DQ305601 Genomic DNA Translation: ABC26137.1
DQ305602 Genomic DNA Translation: ABC26138.1
DQ305603 Genomic DNA Translation: ABC26139.1
DQ305604 Genomic DNA Translation: ABC26140.1
DQ305605 Genomic DNA Translation: ABC26141.1
DQ305606 Genomic DNA Translation: ABC26142.1
DQ305607 Genomic DNA Translation: ABC26143.1
DQ305608 Genomic DNA Translation: ABC26144.1
DQ305609 Genomic DNA Translation: ABC26145.1
DQ305610 Genomic DNA Translation: ABC26146.1
DQ305611 Genomic DNA Translation: ABC26147.1
DQ305612 Genomic DNA Translation: ABC26148.1
DQ305613 Genomic DNA Translation: ABC26149.1
DQ305614 Genomic DNA Translation: ABC26150.1
DQ305615 Genomic DNA Translation: ABC26151.1
DQ305616 Genomic DNA Translation: ABC26152.1
DQ305617 Genomic DNA Translation: ABC26153.1
DQ305618 Genomic DNA Translation: ABC26154.1
DQ305619 Genomic DNA Translation: ABC26155.1
DQ305620 Genomic DNA Translation: ABC26156.1
DQ305621 Genomic DNA Translation: ABC26157.1
DQ305622 Genomic DNA Translation: ABC26158.1
DQ305623 Genomic DNA Translation: ABC26159.1
DQ305624 Genomic DNA Translation: ABC26160.1
DQ305625 Genomic DNA Translation: ABC26161.1
DQ305626 Genomic DNA Translation: ABC26162.1
DQ305627 Genomic DNA Translation: ABC26163.1
DQ305628 Genomic DNA Translation: ABC26164.1
DQ305629 Genomic DNA Translation: ABC26165.1
DQ305630 Genomic DNA Translation: ABC26166.1
DQ305631 Genomic DNA Translation: ABC26167.1
DQ305632 Genomic DNA Translation: ABC26168.1
DQ305633 Genomic DNA Translation: ABC26169.1
DQ305634 Genomic DNA Translation: ABC26170.1
DQ305635 Genomic DNA Translation: ABC26171.1
DQ305636 Genomic DNA Translation: ABC26172.1
DQ305637 Genomic DNA Translation: ABC26173.1
DQ305638 Genomic DNA Translation: ABC26174.1
DQ305639 Genomic DNA Translation: ABC26175.1
DQ305640 Genomic DNA Translation: ABC26176.1
DQ305641 Genomic DNA Translation: ABC26177.1
DQ305642 Genomic DNA Translation: ABC26178.1
DQ305643 Genomic DNA Translation: ABC26179.1
DQ305644 Genomic DNA Translation: ABC26180.1
DQ305645 Genomic DNA Translation: ABC26181.1
DQ305646 Genomic DNA Translation: ABC26182.1
DQ305647 Genomic DNA Translation: ABC26183.1
DQ305648 Genomic DNA Translation: ABC26184.1
DQ305649 Genomic DNA Translation: ABC26185.1
DQ305650 Genomic DNA Translation: ABC26186.1
DQ305651 Genomic DNA Translation: ABC26187.1
DQ305652 Genomic DNA Translation: ABC26188.1
DQ305653 Genomic DNA Translation: ABC26189.1
DQ305654 Genomic DNA Translation: ABC26190.1
DQ305655 Genomic DNA Translation: ABC26191.1
U53473 Genomic DNA Translation: AAA98976.1
AF042740 Genomic DNA Translation: AAC03773.1
AF055874 Genomic DNA Translation: AAC14117.1
AF055875 Genomic DNA Translation: AAC14118.1
AF320309 Genomic DNA Translation: AAG34181.1
AF348074 Genomic DNA Translation: AAK51710.1
AF348075 Genomic DNA Translation: AAK51711.1 Frameshift.
AY230251 Genomic DNA Translation: AAO73561.1
AY230252 Genomic DNA Translation: AAO73562.1
CR407631 mRNA Translation: CAG28559.1
AY331807 Genomic DNA Translation: AAP81164.1
AC025062 Genomic DNA No translation available.
AC120051 Genomic DNA No translation available.
BC015878 mRNA Translation: AAH15878.1
BC067218 mRNA Translation: AAH67218.1
M75163 Genomic DNA Translation: AAA59906.1
CCDSiCCDS6008.1
PIRiB34585
S02788
RefSeqiNP_000006.2, NM_000015.2
XP_016868427.1, XM_017012938.1
UniGeneiHs.2

Genome annotation databases

EnsembliENST00000286479; ENSP00000286479; ENSG00000156006
GeneIDi10
KEGGihsa:10
UCSCiuc003wyw.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiARY2_HUMAN
AccessioniPrimary (citable) accession number: P11245
Secondary accession number(s): O43637
, O60654, O60655, Q13146, Q16697, Q2MLE4, Q2MLF5, Q2MLG8, Q2MLJ6, Q2MLK4, Q2MLK6, Q2MLN7, Q6LET4, Q86XS0, Q86XS1, Q96KY8, Q96T64, Q96T65, Q9H220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 20, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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