UniProtKB - P11234 (RALB_HUMAN)
Protein
Ras-related protein Ral-B
Gene
RALB
Organism
Homo sapiens (Human)
Status
Functioni
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles (By similarity). Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells (By similarity). Required for suppression of apoptosis (PubMed:17875936). In late stages of cytokinesis, upon completion of the bridge formation between dividing cells, mediates exocyst recruitment to the midbody to drive abscission (PubMed:18756269).By similarity2 Publications
Catalytic activityi
- EC:3.6.5.2Curated
Activity regulationi
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 21 – 29 | GTP | 9 | |
Nucleotide bindingi | 68 – 72 | GTP | 5 | |
Nucleotide bindingi | 128 – 131 | GTP | 4 | |
Nucleotide bindingi | 158 – 160 | GTP | 3 |
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- GDP binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cellular response to exogenous dsRNA Source: UniProtKB
- cellular response to starvation Source: UniProtKB
- negative regulation of protein binding Source: UniProtKB
- positive regulation of autophagosome assembly Source: UniProtKB
- positive regulation of protein binding Source: UniProtKB
- positive regulation of protein phosphorylation Source: UniProtKB
- positive regulation of protein serine/threonine kinase activity Source: UniProtKB
- Ras protein signal transduction Source: GO_Central
- regulation of exocyst assembly Source: UniProtKB
- regulation of exocyst localization Source: UniProtKB
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Hydrolase |
Biological process | Apoptosis, Cell cycle, Cell division |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P11234 |
Reactomei | R-HSA-171007, p38MAPK events |
SIGNORi | P11234 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RALB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000144118.13 |
HGNCi | HGNC:9840, RALB |
MIMi | 179551, gene |
neXtProti | NX_P11234 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Other locations
- Midbody 1 Publication
Note: During late cytokinesis, enriched at the midbody.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 11 | Missing : No effect on cytokinesis. Impaired cytokinesis, as shown by increased number of binucleate cells; when associated with V-23. 1 PublicationAdd BLAST | 11 | |
Mutagenesisi | 23 | G → V: Impaired cytokinesis, as shown by increased number of binucleate cells. Impaired cytokinesis; when associated with 1-M--S-11 or N-49. No effect on cytokinesis; when associated with R-38, W-48 or E-49. No effect on interaction with EXOC2 and EXOC8. Decreased interaction with EXOC2 and EXOC8; when associated with R-38 or W-48. 1 Publication | 1 | |
Mutagenesisi | 38 | E → R: No effect on cytokinesis. No effect on cytokinesis; when associated with V-23. Decreased interaction with EXOC2 and EXOC8; when associated with V-23. 1 Publication | 1 | |
Mutagenesisi | 46 | T → A: Reduces the binding affinity to EXOC2 effector. 1 Publication | 1 | |
Mutagenesisi | 46 | T → S: Reduces the binding affinity to EXOC2 effector. 1 Publication | 1 | |
Mutagenesisi | 48 | A → W: Impaired abscission, the last step of cytokinesis, as shown by the accumulation of bridged cells. No effect on cytokinesis; when associated with V-23. Decreased interaction with EXOC2 and EXOC8; when associated with V-23. 1 Publication | 1 | |
Mutagenesisi | 49 | D → E: Impaired abscission, the last step of cytokinesis. No effect on cytokinesis; when associated with V-23. 1 Publication | 1 | |
Mutagenesisi | 49 | D → N: No effect on cytokinesis. Impaired cytokinesis, as shown by increased number of binucleate cells; when associated with V-23. 1 Publication | 1 | |
Mutagenesisi | 72 | Q → L: Loss of GTPase activity. 1 Publication | 1 | |
Mutagenesisi | 203 | C → S: Loss of geranylgeranylation and membrane localization. 1 Publication | 1 | |
Mutagenesisi | 206 | L → S: Converts geranyl-geranylation to farnesylation. No effect on membrane localization. Confers resistance to GGTI-induced pancreatic cancer cell apoptosis, but not to GGTI-dependent inhibition of anchorage-independent proliferation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5899 |
OpenTargetsi | ENSG00000144118 |
PharmGKBi | PA34198 |
Miscellaneous databases
Pharosi | P11234, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3879851 |
Polymorphism and mutation databases
BioMutai | RALB |
DMDMi | 131835 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082698 | 1 – 203 | Ras-related protein Ral-BAdd BLAST | 203 | |
PropeptideiPRO_0000281349 | 204 – 206 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 203 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 203 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).1 Publication
The farnesylated form confers resistance to the proapoptotic and anti-anchorage-dependent growth effects of geranylgeranyltransferase I inhibitors, including GGTI-2417.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | P11234 |
jPOSTi | P11234 |
MassIVEi | P11234 |
PaxDbi | P11234 |
PeptideAtlasi | P11234 |
PRIDEi | P11234 |
ProteomicsDBi | 52727 [P11234-1] 5648 68195 |
PTM databases
iPTMneti | P11234 |
PhosphoSitePlusi | P11234 |
SwissPalmi | P11234 |
Expressioni
Gene expression databases
Bgeei | ENSG00000144118, Expressed in frontal cortex and 243 other tissues |
ExpressionAtlasi | P11234, baseline and differential |
Genevisiblei | P11234, HS |
Organism-specific databases
HPAi | ENSG00000144118, Tissue enhanced (parathyroid) |
Interactioni
Subunit structurei
Binary interactionsi
P11234
With | #Exp. | IntAct |
---|---|---|
INCA1 [Q0VD86] | 3 | EBI-752162,EBI-6509505 |
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111835, 41 interactors |
IntActi | P11234, 48 interactors |
MINTi | P11234 |
STRINGi | 9606.ENSP00000272519 |
Miscellaneous databases
RNActi | P11234, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P11234 |
SMRi | P11234 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11234 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 43 – 51 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155984 |
InParanoidi | P11234 |
OMAi | QNITEMF |
PhylomeDBi | P11234 |
TreeFami | TF312796 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028412, Ral IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF199, PTHR24070:SF199, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P11234-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAANKSKGQS SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS
60 70 80 90 100
YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL LVFSITEHES
110 120 130 140 150
FTATAEFREQ ILRVKAEEDK IPLLVVGNKS DLEERRQVPV EEARSKAEEW
160 170 180 190 200
GVQYVETSAK TRANVDKVFF DLMREIRTKK MSENKDKNGK KSSKNKKSFK
ERCCLL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J6B1 | C9J6B1_HUMAN | Small monomeric GTPase | RALB | 167 | Annotation score: | ||
C9JQB3 | C9JQB3_HUMAN | Small monomeric GTPase | RALB | 171 | Annotation score: | ||
C9JYR1 | C9JYR1_HUMAN | Small monomeric GTPase | RALB | 112 | Annotation score: | ||
F8WEQ6 | F8WEQ6_HUMAN | Ras-related protein Ral-B | RALB | 46 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055843 | 1 | M → MKQRQSALQWVICVSQPQKT SEM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_055844 | 36 – 37 | YD → NVSKSLAYDKKKYTANKKVE GIL in isoform 3. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15015 mRNA Translation: CAA33119.1 M35416 mRNA Translation: AAA60250.1 AF493911 mRNA Translation: AAM12625.1 BT006953 mRNA Translation: AAP35599.1 AK127675 mRNA Translation: BAC87080.1 AK303214 mRNA Translation: BAG64302.1 AK312453 mRNA Translation: BAG35360.1 AC012363 Genomic DNA Translation: AAY14800.1 CH471103 Genomic DNA Translation: EAW95240.1 CH471103 Genomic DNA Translation: EAW95242.1 BC018163 mRNA Translation: AAH18163.1 |
CCDSi | CCDS2131.1 [P11234-1] |
PIRi | S04597, TVHUAB |
RefSeqi | NP_002872.1, NM_002881.2 [P11234-1] XP_005263781.1, XM_005263724.1 XP_005263784.1, XM_005263727.1 XP_005263785.1, XM_005263728.1 [P11234-1] XP_005263786.1, XM_005263729.2 XP_016860111.1, XM_017004622.1 |
Genome annotation databases
Ensembli | ENST00000272519; ENSP00000272519; ENSG00000144118 [P11234-1] ENST00000420510; ENSP00000414224; ENSG00000144118 [P11234-1] |
GeneIDi | 5899 |
KEGGi | hsa:5899 |
UCSCi | uc002tmk.4, human [P11234-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15015 mRNA Translation: CAA33119.1 M35416 mRNA Translation: AAA60250.1 AF493911 mRNA Translation: AAM12625.1 BT006953 mRNA Translation: AAP35599.1 AK127675 mRNA Translation: BAC87080.1 AK303214 mRNA Translation: BAG64302.1 AK312453 mRNA Translation: BAG35360.1 AC012363 Genomic DNA Translation: AAY14800.1 CH471103 Genomic DNA Translation: EAW95240.1 CH471103 Genomic DNA Translation: EAW95242.1 BC018163 mRNA Translation: AAH18163.1 |
CCDSi | CCDS2131.1 [P11234-1] |
PIRi | S04597, TVHUAB |
RefSeqi | NP_002872.1, NM_002881.2 [P11234-1] XP_005263781.1, XM_005263724.1 XP_005263784.1, XM_005263727.1 XP_005263785.1, XM_005263728.1 [P11234-1] XP_005263786.1, XM_005263729.2 XP_016860111.1, XM_017004622.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KE5 | NMR | - | A | 12-185 | [»] | |
2KWI | NMR | - | A | 8-185 | [»] | |
BMRBi | P11234 | |||||
SMRi | P11234 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111835, 41 interactors |
IntActi | P11234, 48 interactors |
MINTi | P11234 |
STRINGi | 9606.ENSP00000272519 |
Chemistry databases
ChEMBLi | CHEMBL3879851 |
PTM databases
iPTMneti | P11234 |
PhosphoSitePlusi | P11234 |
SwissPalmi | P11234 |
Polymorphism and mutation databases
BioMutai | RALB |
DMDMi | 131835 |
Proteomic databases
EPDi | P11234 |
jPOSTi | P11234 |
MassIVEi | P11234 |
PaxDbi | P11234 |
PeptideAtlasi | P11234 |
PRIDEi | P11234 |
ProteomicsDBi | 52727 [P11234-1] 5648 68195 |
Protocols and materials databases
Antibodypediai | 33389, 349 antibodies |
DNASUi | 5899 |
Genome annotation databases
Ensembli | ENST00000272519; ENSP00000272519; ENSG00000144118 [P11234-1] ENST00000420510; ENSP00000414224; ENSG00000144118 [P11234-1] |
GeneIDi | 5899 |
KEGGi | hsa:5899 |
UCSCi | uc002tmk.4, human [P11234-1] |
Organism-specific databases
CTDi | 5899 |
DisGeNETi | 5899 |
EuPathDBi | HostDB:ENSG00000144118.13 |
GeneCardsi | RALB |
HGNCi | HGNC:9840, RALB |
HPAi | ENSG00000144118, Tissue enhanced (parathyroid) |
MIMi | 179551, gene |
neXtProti | NX_P11234 |
OpenTargetsi | ENSG00000144118 |
PharmGKBi | PA34198 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155984 |
InParanoidi | P11234 |
OMAi | QNITEMF |
PhylomeDBi | P11234 |
TreeFami | TF312796 |
Enzyme and pathway databases
PathwayCommonsi | P11234 |
Reactomei | R-HSA-171007, p38MAPK events |
SIGNORi | P11234 |
Miscellaneous databases
BioGRID-ORCSi | 5899, 4 hits in 847 CRISPR screens |
ChiTaRSi | RALB, human |
EvolutionaryTracei | P11234 |
GeneWikii | RALB |
GenomeRNAii | 5899 |
Pharosi | P11234, Tbio |
PROi | PR:P11234 |
RNActi | P11234, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000144118, Expressed in frontal cortex and 243 other tissues |
ExpressionAtlasi | P11234, baseline and differential |
Genevisiblei | P11234, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028412, Ral IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF199, PTHR24070:SF199, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RALB_HUMAN | |
Accessioni | P11234Primary (citable) accession number: P11234 Secondary accession number(s): B4E040, Q53T32, Q6ZS74 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 210 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM