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Protein

Glycogen phosphorylase, brain form

Gene

PYGB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycogen phosphorylase that regulates glycogen mobilization (PubMed:27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed:3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed:3346228). However, all known phosphorylases share catalytic and structural properties (PubMed:3346228).1 Publication1 Publication

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.1 Publication

Cofactori

Activity regulationi

Activity of phosphorylase is controlled both by allosteric means (through the non-covalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B. Activated upon phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43AMP; shared with dimeric partner1 Publication1
Sitei76Participates in a stacking interaction with the adenine ring of AMP1 Publication1
Sitei109Involved in the association of subunitsBy similarity1
Sitei143Involved in the association of subunitsBy similarity1
Sitei156May be involved in allosteric controlBy similarity1
Binding sitei197AMP1 Publication1
Binding sitei310AMP1 Publication1
Binding sitei569Pyridoxal 5'-phosphate1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS02178-MONOMER
ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-70221 Glycogen breakdown (glycogenolysis)

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, brain form1 Publication (EC:2.4.1.11 Publication)
Gene namesi
Name:PYGB1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000100994.11
HGNCiHGNC:9723 PYGB
MIMi138550 gene
neXtProtiNX_P11216

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5834
OpenTargetsiENSG00000100994
PharmGKBiPA34066

Chemistry databases

ChEMBLiCHEMBL3856
DrugBankiDB00114 Pyridoxal Phosphate

Polymorphism and mutation databases

BioMutaiPYGB
DMDMi20178317

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001885352 – 843Glycogen phosphorylase, brain formAdd BLAST842

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei15Phosphoserine; by PHK; in form phosphorylase ABy similarity1
Modified residuei197PhosphotyrosineBy similarity1
Modified residuei473PhosphotyrosineBy similarity1
Modified residuei681N6-(pyridoxal phosphate)lysine1 Publication1

Post-translational modificationi

Phosphorylated (PubMed:27402852). Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A (By similarity).By similarity1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP11216
MaxQBiP11216
PaxDbiP11216
PeptideAtlasiP11216
PRIDEiP11216
ProteomicsDBi52720

PTM databases

iPTMnetiP11216
PhosphoSitePlusiP11216
SwissPalmiP11216

Expressioni

Gene expression databases

BgeeiENSG00000100994 Expressed in 205 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_PYGB
ExpressionAtlasiP11216 baseline and differential
GenevisibleiP11216 HS

Organism-specific databases

HPAiHPA031067

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi111792, 51 interactors
IntActiP11216, 7 interactors
STRINGi9606.ENSP00000216962

Chemistry databases

BindingDBiP11216

Structurei

Secondary structure

1843
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP11216
SMRiP11216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni677 – 678Pyridoxal 5'-phosphate1 Publication2

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
GeneTreeiENSGT00390000016886
HOVERGENiHBG006848
InParanoidiP11216
KOiK00688
OMAiYNAREFY
OrthoDBiEOG091G03RB
PhylomeDBiP11216
TreeFamiTF300309

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P11216-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKPLTDSEK RKQISVRGLA GLGDVAEVRK SFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYFFALAHTV RDHLVGRWIR TQQHYYERDP KRIYYLSLEF YMGRTLQNTM
110 120 130 140 150
VNLGLQNACD EAIYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP
210 220 230 240 250
VHFYGRVEHT PDGVKWLDTQ VVLAMPYDTP VPGYKNNTVN TMRLWSAKAP
260 270 280 290 300
NDFKLQDFNV GDYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDIIR RFKSSKFGCR DPVRTCFETF PDKVAIQLND THPALSIPEL
360 370 380 390 400
MRILVDVEKV DWDKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH
410 420 430 440 450
LEIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS
460 470 480 490 500
HAVNGVARIH SEIVKQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADTIVEKIGE EFLTDLSQLK KLLPLVSDEV FIRDVAKVKQ ENKLKFSAFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVVTLYNR IKRDPAKAFV
610 620 630 640 650
PRTVMIGGKA APGYHMAKLI IKLVTSIGDV VNHDPVVGDR LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGAENLF IFGLRVEDVE ALDRKGYNAR EYYDHLPELK QAVDQISSGF
760 770 780 790 800
FSPKEPDCFK DIVNMLMHHD RFKVFADYEA YMQCQAQVDQ LYRNPKEWTK
810 820 830 840
KVIRNIACSG KFSSDRTITE YAREIWGVEP SDLQIPPPNI PRD
Length:843
Mass (Da):96,696
Last modified:January 23, 2007 - v5
Checksum:i810BFAD3002CACB0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y4Z6H0Y4Z6_HUMAN
Alpha-1,4 glucan phosphorylase
PYGB
245Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 3AK → GE (PubMed:3346228).Curated2
Sequence conflicti2 – 3AK → GE (PubMed:2615594).Curated2
Sequence conflicti248K → R in AAA59597 (PubMed:3346228).Curated1
Sequence conflicti302A → G in AAA59597 (PubMed:3346228).Curated1
Sequence conflicti835 – 843IPPPNIPRD → LQHLPHPEWESGGATCWAPP ELCTHLAMY in AAA59597 (PubMed:3346228).Curated9

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034428303A → S. Corresponds to variant dbSNP:rs2228976Ensembl.1
Natural variantiVAR_020212502D → N. Corresponds to variant dbSNP:rs2227891Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03544 mRNA Translation: AAA59597.1
U47025 mRNA Translation: AAB60395.1
AL121772 Genomic DNA No translation available.
BC017045 mRNA Translation: AAH17045.1
BC030795 mRNA Translation: AAH30795.1
CCDSiCCDS13171.1
PIRiA40138
RefSeqiNP_002853.2, NM_002862.3
UniGeneiHs.368157

Genome annotation databases

EnsembliENST00000216962; ENSP00000216962; ENSG00000100994
GeneIDi5834
KEGGihsa:5834
UCSCiuc002wup.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03544 mRNA Translation: AAA59597.1
U47025 mRNA Translation: AAB60395.1
AL121772 Genomic DNA No translation available.
BC017045 mRNA Translation: AAH17045.1
BC030795 mRNA Translation: AAH30795.1
CCDSiCCDS13171.1
PIRiA40138
RefSeqiNP_002853.2, NM_002862.3
UniGeneiHs.368157

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IKOX-ray2.50A1-843[»]
5IKPX-ray3.40A1-843[»]
ProteinModelPortaliP11216
SMRiP11216
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111792, 51 interactors
IntActiP11216, 7 interactors
STRINGi9606.ENSP00000216962

Chemistry databases

BindingDBiP11216
ChEMBLiCHEMBL3856
DrugBankiDB00114 Pyridoxal Phosphate

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

PTM databases

iPTMnetiP11216
PhosphoSitePlusiP11216
SwissPalmiP11216

Polymorphism and mutation databases

BioMutaiPYGB
DMDMi20178317

Proteomic databases

EPDiP11216
MaxQBiP11216
PaxDbiP11216
PeptideAtlasiP11216
PRIDEiP11216
ProteomicsDBi52720

Protocols and materials databases

DNASUi5834
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216962; ENSP00000216962; ENSG00000100994
GeneIDi5834
KEGGihsa:5834
UCSCiuc002wup.4 human

Organism-specific databases

CTDi5834
DisGeNETi5834
EuPathDBiHostDB:ENSG00000100994.11
GeneCardsiPYGB
HGNCiHGNC:9723 PYGB
HPAiHPA031067
MIMi138550 gene
neXtProtiNX_P11216
OpenTargetsiENSG00000100994
PharmGKBiPA34066
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
GeneTreeiENSGT00390000016886
HOVERGENiHBG006848
InParanoidiP11216
KOiK00688
OMAiYNAREFY
OrthoDBiEOG091G03RB
PhylomeDBiP11216
TreeFamiTF300309

Enzyme and pathway databases

BioCyciMetaCyc:HS02178-MONOMER
ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-70221 Glycogen breakdown (glycogenolysis)

Miscellaneous databases

ChiTaRSiPYGB human
GenomeRNAii5834
PROiPR:P11216
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100994 Expressed in 205 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_PYGB
ExpressionAtlasiP11216 baseline and differential
GenevisibleiP11216 HS

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPYGB_HUMAN
AccessioniPrimary (citable) accession number: P11216
Secondary accession number(s): Q96AK1, Q9NPX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 206 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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