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UniProtKB - P11204 (POL_EIAV9)
Protein
Pol polyprotein
Gene
pol
Organism
Equine infectious anemia virus (isolate 1369) (EIAV)
Status
Functioni
During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activityi
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)PROSITE-ProRule annotationEC:2.7.7.49PROSITE-ProRule annotation
- Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. EC:3.1.26.13
- 3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. EC:3.1.13.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 105 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 887 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 891 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 915 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 918 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 878 – 919 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 42 | |
DNA bindingi | 1096 – 1144 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- dUTP diphosphatase activity Source: CAFA
- exoribonuclease H activity Source: UniProtKB-EC
- magnesium ion binding Source: CAFA
- RNA-directed DNA polymerase activity Source: UniProtKB-KW
- RNA-DNA hybrid ribonuclease activity Source: InterPro
- UTP binding Source: CAFA
- zinc ion binding Source: InterPro
GO - Biological processi
- DNA integration Source: UniProtKB-KW
- DNA recombination Source: UniProtKB-KW
- dUMP biosynthetic process Source: CAFA
- dUTP catabolic process Source: CAFA
- establishment of integrated proviral latency Source: UniProtKB-KW
- viral entry into host cell Source: UniProtKB-KW
- viral genome integration into host DNA Source: UniProtKB-KW
Keywordsi
Molecular function | Aspartyl protease, DNA-binding, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase |
Biological process | DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | A02.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Pol polyproteinCleaved into the following 3 chains: |
Gene namesi | Name:pol |
Organismi | Equine infectious anemia virus (isolate 1369) (EIAV) |
Taxonomic identifieri | 11670 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Lentivirus › |
Virus hosti | Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793] Equus caballus (Horse) [TaxID: 9796] |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000038838 | 1 – 195 | ProteaseAdd BLAST | 195 | |
ChainiPRO_0000038839 | 196 – 914 | Reverse transcriptase/ribonuclease HAdd BLAST | 719 | |
ChainiPRO_0000038840 | 915 – 1146 | IntegraseAdd BLAST | 232 |
Post-translational modificationi
Specific enzymatic cleavages in vivo yield mature proteins.
Keywords - PTMi
Cleavage on pair of basic residuesStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P11204 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11204 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 100 – 174 | Peptidase A2PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 230 – 419 | Reverse transcriptasePROSITE-ProRule annotationAdd BLAST | 190 | |
Domaini | 617 – 740 | RNase H type-1PROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 921 – 1078 | Integrase catalyticPROSITE-ProRule annotationAdd BLAST | 158 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 77 | DisorderedSequence analysisAdd BLAST | 65 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 48 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 62 – 77 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the retroviral Pol polyprotein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 878 – 919 | Integrase-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerFamily and domain databases
CDDi | cd07557, trimeric_dUTPase, 1 hit |
Gene3Di | 1.10.10.200, 1 hit 2.30.30.10, 1 hit 2.40.70.10, 1 hit 2.70.40.10, 1 hit 3.30.420.10, 2 hits 3.30.70.270, 3 hits |
InterProi | View protein in InterPro IPR001969, Aspartic_peptidase_AS IPR043502, DNA/RNA_pol_sf IPR029054, dUTPase-like IPR036157, dUTPase-like_sf IPR033704, dUTPase_trimeric IPR017856, Integrase-like_N IPR036862, Integrase_C_dom_sf_retrovir IPR001037, Integrase_C_retrovir IPR001584, Integrase_cat-core IPR003308, Integrase_Zn-bd_dom_N IPR001995, Peptidase_A2_cat IPR021109, Peptidase_aspartic_dom_sf IPR018061, Retropepsins IPR043128, Rev_trsase/Diguanyl_cyclase IPR012337, RNaseH-like_sf IPR002156, RNaseH_domain IPR036397, RNaseH_sf IPR000477, RT_dom IPR010659, RVT_connect IPR010661, RVT_thumb |
Pfami | View protein in Pfam PF00692, dUTPase, 1 hit PF00552, IN_DBD_C, 1 hit PF02022, Integrase_Zn, 1 hit PF00075, RNase_H, 1 hit PF00665, rve, 1 hit PF00077, RVP, 1 hit PF00078, RVT_1, 1 hit PF06815, RVT_connect, 1 hit PF06817, RVT_thumb, 1 hit |
SUPFAMi | SSF46919, SSF46919, 1 hit SSF50122, SSF50122, 1 hit SSF50630, SSF50630, 1 hit SSF51283, SSF51283, 1 hit SSF53098, SSF53098, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50175, ASP_PROT_RETROV, 1 hit PS00141, ASP_PROTEASE, 1 hit PS50994, INTEGRASE, 1 hit PS51027, INTEGRASE_DBD, 1 hit PS50879, RNASE_H_1, 1 hit PS50878, RT_POL, 1 hit PS50876, ZF_INTEGRASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P11204-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
TAWTFLKAMQ KCSKKREARG SREAPETNFP DTTEESAQQI CCTRDSSDSK
60 70 80 90 100
SVPRSERNKK GIQCQGEGSS RGSQPGQFVG VTYNLEKRPT TIVLINDTPL
110 120 130 140 150
NVLLDTGADT SVLTTAHYNR LKYRGRKYQG TGIIGVGGNV ETFSTPVTIK
160 170 180 190 200
KKGRHIKTRM LVADIPVTIL GRDILQDLGA KLVLAQLSKE IKFRKIELKE
210 220 230 240 250
GTMGPKIPQW PLTKEKLEGA KEIVQRLLSE GKISEASDNN PYNSPIFVIK
260 270 280 290 300
KRSGKWRLLQ DLRELNKTVQ VGTEISRGLP HPGGLIKCKH MTVLDIGDAY
310 320 330 340 350
FTIPLDPEFR PYTAFTIPSI NHQEPDKRYV WNCLPQGFVL SPYIYQKTLQ
360 370 380 390 400
EILQPFRERY PEVQLYQYMD DLFVGSNGSK KQHKELIIEL RAILLEEGFE
410 420 430 440 450
TPDDKLQEVP PYSWLGYQLC PENWKVQKMQ LDMVKNPTLN DVQKLMGNIT
460 470 480 490 500
WMSSGVPGLT VKHIAATTKG CLELNQKVIW TEEAQKELEE NNEKIKNAQG
510 520 530 540 550
LQYYNPEEEM LCEVEITKNY EATYVIKQSQ GILWAGKKIM KANKGWSTVK
560 570 580 590 600
NLMLLLQHVA TESITRVGKC PTFKVPFTKE QVMWEMQKGW YYSWLPEIVY
610 620 630 640 650
THQVVHDDWR MKLVEEPTSG ITIYTDGGKQ NGEGIAAYVT SNGRTKQKRL
660 670 680 690 700
GPVTHQVAER MAIQMALEDT RDKQVNIVTD SYYCWKNITE GLGLEGPQSP
710 720 730 740 750
WWPIIQNIRE KEIVYFAWVP GHKGICGNQL ADEAAKIKEE IMLAYQGTQI
760 770 780 790 800
KEKRDEDAGF DLCVPYDIMI PVSDTKIIPT DVKIQVPPNS FGWVTGKSSM
810 820 830 840 850
AKQGLLINGG IIDEGYTGEI QVICTNIGKS NIKLIEGQKF AQLIILQHHS
860 870 880 890 900
NSRQPWDENK ISQRGDKGFG STGVFWVENI QEAQDEHENW HTSPKILARN
910 920 930 940 950
YKIPLTVAKQ ITQECPHCTK QGSGPAGCVM RSPNHWQADC THLDNKIILT
960 970 980 990 1000
FVESNSGYIH ATLLSKENAL CTSLAILEWA RLFSPKSLHT DNGTNFVAEP
1010 1020 1030 1040 1050
VVNLLKFLKI AHTTGIPYHP ESQGIVERAN RTLKEKIQSH RDNTQTLEAA
1060 1070 1080 1090 1100
LQLALITCNK GRESMGGQTP WEVFITNQAQ VIHEKLLLQQ AQSSKKFCFY
1110 1120 1130 1140
KIPGEHDWKG PTRVLWKGDG AVVVNDEGKG IIAVPLTRTK LLIKPN
Sequence cautioni
The sequence AAB59862 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16575 Genomic RNA Translation: AAB59862.1 Different initiation. |
PIRi | B27842, GNLJEW |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16575 Genomic RNA Translation: AAB59862.1 Different initiation. |
PIRi | B27842, GNLJEW |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DUC | X-ray | 2.05 | A | 742-875 | [»] | |
1DUN | X-ray | 1.90 | A | 742-875 | [»] | |
SMRi | P11204 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | A02.004 |
Miscellaneous databases
EvolutionaryTracei | P11204 |
Family and domain databases
CDDi | cd07557, trimeric_dUTPase, 1 hit |
Gene3Di | 1.10.10.200, 1 hit 2.30.30.10, 1 hit 2.40.70.10, 1 hit 2.70.40.10, 1 hit 3.30.420.10, 2 hits 3.30.70.270, 3 hits |
InterProi | View protein in InterPro IPR001969, Aspartic_peptidase_AS IPR043502, DNA/RNA_pol_sf IPR029054, dUTPase-like IPR036157, dUTPase-like_sf IPR033704, dUTPase_trimeric IPR017856, Integrase-like_N IPR036862, Integrase_C_dom_sf_retrovir IPR001037, Integrase_C_retrovir IPR001584, Integrase_cat-core IPR003308, Integrase_Zn-bd_dom_N IPR001995, Peptidase_A2_cat IPR021109, Peptidase_aspartic_dom_sf IPR018061, Retropepsins IPR043128, Rev_trsase/Diguanyl_cyclase IPR012337, RNaseH-like_sf IPR002156, RNaseH_domain IPR036397, RNaseH_sf IPR000477, RT_dom IPR010659, RVT_connect IPR010661, RVT_thumb |
Pfami | View protein in Pfam PF00692, dUTPase, 1 hit PF00552, IN_DBD_C, 1 hit PF02022, Integrase_Zn, 1 hit PF00075, RNase_H, 1 hit PF00665, rve, 1 hit PF00077, RVP, 1 hit PF00078, RVT_1, 1 hit PF06815, RVT_connect, 1 hit PF06817, RVT_thumb, 1 hit |
SUPFAMi | SSF46919, SSF46919, 1 hit SSF50122, SSF50122, 1 hit SSF50630, SSF50630, 1 hit SSF51283, SSF51283, 1 hit SSF53098, SSF53098, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50175, ASP_PROT_RETROV, 1 hit PS00141, ASP_PROTEASE, 1 hit PS50994, INTEGRASE, 1 hit PS51027, INTEGRASE_DBD, 1 hit PS50879, RNASE_H_1, 1 hit PS50878, RT_POL, 1 hit PS50876, ZF_INTEGRASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POL_EIAV9 | |
Accessioni | P11204Primary (citable) accession number: P11204 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | September 29, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families