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Protein

Ribonucleoside-diphosphate reductase subunit M2

Gene

Rrm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling (By similarity).By similarity

Miscellaneous

Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationNote: Binds 2 iron ions per subunit.

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Iron 11
Metal bindingi170Iron 11
Metal bindingi170Iron 21
Metal bindingi173Iron 11
Active sitei177PROSITE-ProRule annotation1
Metal bindingi233Iron 21
Metal bindingi267Iron 11
Metal bindingi267Iron 21
Metal bindingi270Iron 21

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleotide biosynthetic process Source: UniProtKB
  • deoxyribonucleotide metabolic process Source: MGI
  • DNA replication Source: UniProtKB-UniPathway
  • protein complex oligomerization Source: MGI
  • protein heterotetramerization Source: CAFA

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.17.4.1 3474
ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayi
UPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small chain
Ribonucleotide reductase small subunit
Gene namesi
Name:Rrm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:98181 Rrm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3527

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904501 – 390Ribonucleoside-diphosphate reductase subunit M2Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineCombined sources1
Modified residuei33PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP11157
MaxQBiP11157
PaxDbiP11157
PeptideAtlasiP11157
PRIDEiP11157

PTM databases

iPTMnetiP11157
PhosphoSitePlusiP11157

Expressioni

Gene expression databases

BgeeiENSMUSG00000020649 Expressed in 280 organ(s), highest expression level in decidua
CleanExiMM_RRM2
ExpressionAtlasiP11157 baseline and differential
GenevisibleiP11157 MM

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203023, 4 interactors
ComplexPortaliCPX-370 Ribonucleoside-diphosphate reductase RR1 complex, RRM2 variant
IntActiP11157, 1 interactor
STRINGi10090.ENSMUSP00000020980

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00462
ProteinModelPortaliP11157
SMRiP11157
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11157

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
GeneTreeiENSGT00390000013305
HOGENOMiHOG000255975
HOVERGENiHBG001647
InParanoidiP11157
KOiK10808
OMAiCNRRCQQ
OrthoDBiEOG091G0AZQ
PhylomeDBiP11157
TreeFamiTF300465

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P11157-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSVRTPLAT IADQQQLQLS PLKRLTLADK ENTPPTLSST RVLASKAARR
60 70 80 90 100
IFQDSAELES KAPTNPSVED EPLLRENPRR FVVFPIEYHD IWQMYKKAEA
110 120 130 140 150
SFWTAEEVDL SKDIQHWEAL KPDERHFISH VLAFFAASDG IVNENLVERF
160 170 180 190 200
SQEVQVTEAR CFYGFQIAME NIHSEMYSLL IDTYIKDPKE REYLFNAIET
210 220 230 240 250
MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS FASIFWLKKR
260 270 280 290 300
GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPAEQRV REIITNAVRI
310 320 330 340 350
EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFNK IFRVENPFDF
360 370 380 390
MENISLEGKT NFFEKRVGEY QRMGVMSNST ENSFTLDADF
Length:390
Mass (Da):45,096
Last modified:July 1, 1989 - v1
Checksum:iAC7ACC4FAF8A4A2F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z0D8D3Z0D8_MOUSE
Ribonucleoside-diphosphate reductas...
Rrm2
225Annotation score:
F6RUC3F6RUC3_MOUSE
Ribonucleoside-diphosphate reductas...
Rrm2
243Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14223 mRNA Translation: AAA40062.1
X15666 Genomic DNA Translation: CAA33707.1
AK088907 mRNA Translation: BAC40647.1
AK142027 mRNA Translation: BAE24918.1
AK147111 mRNA Translation: BAE27683.1
AK161643 mRNA Translation: BAE36508.1
AK167078 mRNA Translation: BAE39238.1
AK167204 mRNA Translation: BAE39331.1
AK168205 mRNA Translation: BAE40165.1
AK168994 mRNA Translation: BAE40793.1
BC085136 mRNA Translation: AAH85136.1
CCDSiCCDS25844.1
PIRiS06735
RefSeqiNP_033130.1, NM_009104.2
UniGeneiMm.471826
Mm.99

Genome annotation databases

EnsembliENSMUST00000020980; ENSMUSP00000020980; ENSMUSG00000020649
GeneIDi20135
KEGGimmu:20135
UCSCiuc007ner.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14223 mRNA Translation: AAA40062.1
X15666 Genomic DNA Translation: CAA33707.1
AK088907 mRNA Translation: BAC40647.1
AK142027 mRNA Translation: BAE24918.1
AK147111 mRNA Translation: BAE27683.1
AK161643 mRNA Translation: BAE36508.1
AK167078 mRNA Translation: BAE39238.1
AK167204 mRNA Translation: BAE39331.1
AK168205 mRNA Translation: BAE40165.1
AK168994 mRNA Translation: BAE40793.1
BC085136 mRNA Translation: AAH85136.1
CCDSiCCDS25844.1
PIRiS06735
RefSeqiNP_033130.1, NM_009104.2
UniGeneiMm.471826
Mm.99

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AFTNMR-A384-390[»]
1H0NX-ray2.40A1-390[»]
1H0OX-ray2.20A1-390[»]
1W68X-ray2.20A1-390[»]
1W69X-ray2.20A1-390[»]
1XSMX-ray2.30A1-390[»]
DisProtiDP00462
ProteinModelPortaliP11157
SMRiP11157
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203023, 4 interactors
ComplexPortaliCPX-370 Ribonucleoside-diphosphate reductase RR1 complex, RRM2 variant
IntActiP11157, 1 interactor
STRINGi10090.ENSMUSP00000020980

Chemistry databases

ChEMBLiCHEMBL3527

PTM databases

iPTMnetiP11157
PhosphoSitePlusiP11157

Proteomic databases

EPDiP11157
MaxQBiP11157
PaxDbiP11157
PeptideAtlasiP11157
PRIDEiP11157

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020980; ENSMUSP00000020980; ENSMUSG00000020649
GeneIDi20135
KEGGimmu:20135
UCSCiuc007ner.2 mouse

Organism-specific databases

CTDi6241
MGIiMGI:98181 Rrm2

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
GeneTreeiENSGT00390000013305
HOGENOMiHOG000255975
HOVERGENiHBG001647
InParanoidiP11157
KOiK10808
OMAiCNRRCQQ
OrthoDBiEOG091G0AZQ
PhylomeDBiP11157
TreeFamiTF300465

Enzyme and pathway databases

UniPathwayi
UPA00326

BRENDAi1.17.4.1 3474
ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Miscellaneous databases

ChiTaRSiRrm2 mouse
EvolutionaryTraceiP11157
PROiPR:P11157
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020649 Expressed in 280 organ(s), highest expression level in decidua
CleanExiMM_RRM2
ExpressionAtlasiP11157 baseline and differential
GenevisibleiP11157 MM

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIR2_MOUSE
AccessioniPrimary (citable) accession number: P11157
Secondary accession number(s): Q3UI23, Q542E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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