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UniProtKB - P11071 (ACEK_ECOLI)
Protein
Isocitrate dehydrogenase kinase/phosphatase
Gene
aceK
Organism
Escherichia coli (strain K12)
Status
Functioni
Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.
Catalytic activityi
- ATP + L-seryl-[isocitrate dehydrogenase] = ADP + H+ + O-phospho-L-seryl-[isocitrate dehydrogenase]UniRule annotationEC:2.7.11.5UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 336 | ATPUniRule annotation | 1 | |
Active sitei | 371 | Curated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 315 – 321 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- [isocitrate dehydrogenase (NADP+)] kinase activity Source: EcoCyc
- AMP binding Source: EcoCyc
- ATP binding Source: EcoliWiki
- phosphoprotein phosphatase activity Source: GO_Central
- protein serine/threonine kinase activity Source: EcoCyc
- protein serine/threonine phosphatase activity Source: EcoCyc
GO - Biological processi
- glucose metabolic process Source: InterPro
- glyoxylate cycle Source: EcoliWiki
- peptidyl-serine dephosphorylation Source: EcoCyc
- peptidyl-serine phosphorylation Source: EcoCyc
- protein dephosphorylation Source: GO_Central
- regulation of catalytic activity Source: EcoCyc
- tricarboxylic acid cycle Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ICITDEHASE-KIN-PHOSPHA |
BRENDAi | 2.7.11.5, 2026 |
SABIO-RKi | P11071 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aceKUniRule annotation Ordered Locus Names:b4016, JW3976 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: EcoliWiki
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 336 | K → M: Inhibits enzyme. 1 Publication | 1 | |
Mutagenesisi | 371 | D → A, E or Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 377 | N → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 403 | D → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 439 | E → A: No loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057898 | 1 – 578 | Isocitrate dehydrogenase kinase/phosphataseAdd BLAST | 578 |
Proteomic databases
jPOSTi | P11071 |
PaxDbi | P11071 |
PRIDEi | P11071 |
Interactioni
Binary interactionsi
P11071
With | #Exp. | IntAct |
---|---|---|
icd [P08200] | 5 | EBI-1112800,EBI-369069 |
Protein-protein interaction databases
BioGRIDi | 4261467, 11 interactors 849198, 7 interactors |
DIPi | DIP-9041N |
IntActi | P11071, 12 interactors |
STRINGi | 511145.b4016 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P11071 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the AceK family.UniRule annotation
Phylogenomic databases
eggNOGi | COG4579, Bacteria |
HOGENOMi | CLU_033804_1_1_6 |
InParanoidi | P11071 |
PhylomeDBi | P11071 |
Family and domain databases
HAMAPi | MF_00747, AceK, 1 hit |
InterProi | View protein in InterPro IPR010452, Isocitrate_DH_AceK |
PANTHERi | PTHR39559, PTHR39559, 1 hit |
Pfami | View protein in Pfam PF06315, AceK, 1 hit |
PIRSFi | PIRSF000719, AceK, 1 hit |
i Sequence
Sequence statusi: Complete.
P11071-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE
160 170 180 190 200
SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VRYGEMLF
Sequence cautioni
The sequence AAA24007 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 | A → G in AAA24007 (PubMed:2826408).Curated | 1 | |
Sequence conflicti | 416 – 417 | ER → DG in AAA24010 (PubMed:2836370).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20714 Genomic DNA Translation: AAA24010.1 M18974 Genomic DNA Translation: AAA24007.1 Frameshift. U00006 Genomic DNA Translation: AAC43110.1 U00096 Genomic DNA Translation: AAC76986.1 AP009048 Genomic DNA Translation: BAE78018.1 M22621 Genomic DNA Translation: AAC13651.1 M63497 Genomic DNA Translation: AAA73005.1 |
PIRi | G65208, KIECID |
RefSeqi | NP_418440.1, NC_000913.3 WP_001137220.1, NZ_SSZK01000049.1 |
Genome annotation databases
EnsemblBacteriai | AAC76986; AAC76986; b4016 BAE78018; BAE78018; BAE78018 |
GeneIDi | 944797 |
KEGGi | ecj:JW3976 eco:b4016 |
PATRICi | fig|1411691.4.peg.2697 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20714 Genomic DNA Translation: AAA24010.1 M18974 Genomic DNA Translation: AAA24007.1 Frameshift. U00006 Genomic DNA Translation: AAC43110.1 U00096 Genomic DNA Translation: AAC76986.1 AP009048 Genomic DNA Translation: BAE78018.1 M22621 Genomic DNA Translation: AAC13651.1 M63497 Genomic DNA Translation: AAA73005.1 |
PIRi | G65208, KIECID |
RefSeqi | NP_418440.1, NC_000913.3 WP_001137220.1, NZ_SSZK01000049.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6K5L | X-ray | 2.55 | A/B | 1-578 | [»] | |
SMRi | P11071 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261467, 11 interactors 849198, 7 interactors |
DIPi | DIP-9041N |
IntActi | P11071, 12 interactors |
STRINGi | 511145.b4016 |
Proteomic databases
jPOSTi | P11071 |
PaxDbi | P11071 |
PRIDEi | P11071 |
Protocols and materials databases
DNASUi | 944797 |
Genome annotation databases
EnsemblBacteriai | AAC76986; AAC76986; b4016 BAE78018; BAE78018; BAE78018 |
GeneIDi | 944797 |
KEGGi | ecj:JW3976 eco:b4016 |
PATRICi | fig|1411691.4.peg.2697 |
Organism-specific databases
EchoBASEi | EB0025 |
Phylogenomic databases
eggNOGi | COG4579, Bacteria |
HOGENOMi | CLU_033804_1_1_6 |
InParanoidi | P11071 |
PhylomeDBi | P11071 |
Enzyme and pathway databases
BioCyci | EcoCyc:ICITDEHASE-KIN-PHOSPHA |
BRENDAi | 2.7.11.5, 2026 |
SABIO-RKi | P11071 |
Miscellaneous databases
PROi | PR:P11071 |
Family and domain databases
HAMAPi | MF_00747, AceK, 1 hit |
InterProi | View protein in InterPro IPR010452, Isocitrate_DH_AceK |
PANTHERi | PTHR39559, PTHR39559, 1 hit |
Pfami | View protein in Pfam PF06315, AceK, 1 hit |
PIRSFi | PIRSF000719, AceK, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEK_ECOLI | |
Accessioni | P11071Primary (citable) accession number: P11071 Secondary accession number(s): P11070, Q2M6T8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families