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Protein

Isocitrate dehydrogenase kinase/phosphatase

Gene

aceK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.

Catalytic activityi

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei336ATPUniRule annotation1
Active sitei371Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi315 – 321ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ICITDEHASE-KIN-PHOSPHA
MetaCyc:ICITDEHASE-KIN-PHOSPHA
BRENDAi2.7.11.5 2026
SABIO-RKiP11071

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation)
Short name:
IDH kinase/phosphataseUniRule annotation
Short name:
IDHK/PUniRule annotation
Gene namesi
Name:aceKUniRule annotation
Ordered Locus Names:b4016, JW3976
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10026 aceK

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi336K → M: Inhibits enzyme. 1 Publication1
Mutagenesisi371D → A, E or Q: Loss of activity. 1 Publication1
Mutagenesisi377N → A: No loss of activity. 1 Publication1
Mutagenesisi403D → A: No loss of activity. 1 Publication1
Mutagenesisi439E → A: No loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000578981 – 578Isocitrate dehydrogenase kinase/phosphataseAdd BLAST578

Proteomic databases

PaxDbiP11071
PRIDEiP11071

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
icdP082005EBI-1112800,EBI-369069

Protein-protein interaction databases

BioGridi4261467, 11 interactors
DIPiDIP-9041N
IntActiP11071, 12 interactors
STRINGi316385.ECDH10B_4205

Structurei

3D structure databases

ProteinModelPortaliP11071
SMRiP11071
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105VS7 Bacteria
COG4579 LUCA
HOGENOMiHOG000247673
InParanoidiP11071
KOiK00906

Family and domain databases

HAMAPiMF_00747 AceK, 1 hit
InterProiView protein in InterPro
IPR010452 Isocitrate_DH_AceK
PANTHERiPTHR39559 PTHR39559, 1 hit
PfamiView protein in Pfam
PF06315 AceK, 1 hit
PIRSFiPIRSF000719 AceK, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD043552 Isocitrate_DH_AceK, 1 hit

Sequencei

Sequence statusi: Complete.

P11071-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR
60 70 80 90 100
IHLYDHHVGL VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE
110 120 130 140 150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE
160 170 180 190 200
SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL
210 220 230 240 250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF
260 270 280 290 300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE
310 320 330 340 350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA
360 370 380 390 400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK
410 420 430 440 450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA
460 470 480 490 500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL
510 520 530 540 550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN
560 570
RIREGHVEDV YAYRRRQRFS VRYGEMLF
Length:578
Mass (Da):67,699
Last modified:December 1, 2000 - v2
Checksum:i5ABD5FB5FA1D9B29
GO

Sequence cautioni

The sequence AAA24007 differs from that shown. Reason: Frameshift at positions 92, 137 and 166.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140A → G in AAA24007 (PubMed:2826408).Curated1
Sequence conflicti416 – 417ER → DG in AAA24010 (PubMed:2836370).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20714 Genomic DNA Translation: AAA24010.1
M18974 Genomic DNA Translation: AAA24007.1 Frameshift.
U00006 Genomic DNA Translation: AAC43110.1
U00096 Genomic DNA Translation: AAC76986.1
AP009048 Genomic DNA Translation: BAE78018.1
M22621 Genomic DNA Translation: AAC13651.1
M63497 Genomic DNA Translation: AAA73005.1
PIRiG65208 KIECID
RefSeqiNP_418440.1, NC_000913.3
WP_001137220.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76986; AAC76986; b4016
BAE78018; BAE78018; BAE78018
GeneIDi944797
KEGGiecj:JW3976
eco:b4016
PATRICifig|1411691.4.peg.2697

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20714 Genomic DNA Translation: AAA24010.1
M18974 Genomic DNA Translation: AAA24007.1 Frameshift.
U00006 Genomic DNA Translation: AAC43110.1
U00096 Genomic DNA Translation: AAC76986.1
AP009048 Genomic DNA Translation: BAE78018.1
M22621 Genomic DNA Translation: AAC13651.1
M63497 Genomic DNA Translation: AAA73005.1
PIRiG65208 KIECID
RefSeqiNP_418440.1, NC_000913.3
WP_001137220.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP11071
SMRiP11071
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261467, 11 interactors
DIPiDIP-9041N
IntActiP11071, 12 interactors
STRINGi316385.ECDH10B_4205

Proteomic databases

PaxDbiP11071
PRIDEiP11071

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76986; AAC76986; b4016
BAE78018; BAE78018; BAE78018
GeneIDi944797
KEGGiecj:JW3976
eco:b4016
PATRICifig|1411691.4.peg.2697

Organism-specific databases

EchoBASEiEB0025
EcoGeneiEG10026 aceK

Phylogenomic databases

eggNOGiENOG4105VS7 Bacteria
COG4579 LUCA
HOGENOMiHOG000247673
InParanoidiP11071
KOiK00906

Enzyme and pathway databases

BioCyciEcoCyc:ICITDEHASE-KIN-PHOSPHA
MetaCyc:ICITDEHASE-KIN-PHOSPHA
BRENDAi2.7.11.5 2026
SABIO-RKiP11071

Miscellaneous databases

PROiPR:P11071

Family and domain databases

HAMAPiMF_00747 AceK, 1 hit
InterProiView protein in InterPro
IPR010452 Isocitrate_DH_AceK
PANTHERiPTHR39559 PTHR39559, 1 hit
PfamiView protein in Pfam
PF06315 AceK, 1 hit
PIRSFiPIRSF000719 AceK, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD043552 Isocitrate_DH_AceK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACEK_ECOLI
AccessioniPrimary (citable) accession number: P11071
Secondary accession number(s): P11070, Q2M6T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 1, 2000
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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