aceK

Functionⁱ

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation.

Catalytic activityⁱ

ATP + [isocitrate dehydrogenase (NADP⁺)] = ADP + [isocitrate dehydrogenase (NADP⁺)] phosphate.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	336	ATPUniRule annotation		1
Active siteⁱ	371	Curated		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	315 – 321	ATPUniRule annotation		7

GO - Molecular functionⁱ

[isocitrate dehydrogenase (NADP+)] kinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 6292732
AMP binding
Source: EcoCyc
Inferred from direct assayⁱ
- 20505668
ATP binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 6292732
protein serine/threonine kinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 3112144
protein serine/threonine phosphatase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 8702587

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glucose metabolic process Source: InterPro
glyoxylate cycle
Source: EcoliWiki
Inferred by curatorⁱ
- 6292732
peptidyl-serine dephosphorylation
Source: EcoCyc
Inferred from direct assayⁱ
- 6292732
peptidyl-serine phosphorylation
Source: EcoCyc
Inferred from direct assayⁱ
- 3112144
regulation of catalytic activity
Source: EcoCyc
Inferred from direct assayⁱ
- 6292732
tricarboxylic acid cycle
Source: EcoliWiki
Inferred by curatorⁱ
- 6292732

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ICITDEHASE-KIN-PHOSPHA MetaCyc:ICITDEHASE-KIN-PHOSPHA
BRENDAⁱ	2.7.11.5 2026
SABIO-RKⁱ	P11071

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Isocitrate dehydrogenase kinase/phosphataseUniRule annotation (EC:2.7.11.5UniRule annotation, EC:3.1.3.-UniRule annotation) Short name: IDH kinase/phosphataseUniRule annotation Short name: IDHK/PUniRule annotation
Gene namesⁱ	Name:aceKUniRule annotation Ordered Locus Names:b4016, JW3976
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10026 aceK

EcoGeneⁱ

EG10026 aceK

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytoplasm
Source: EcoliWiki
Inferred by curatorⁱ
- 6292732

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	336	K → M: Inhibits enzyme. 1 Publication	1
Mutagenesisⁱ	371	D → A, E or Q: Loss of activity. 1 Publication	1
Mutagenesisⁱ	377	N → A: No loss of activity. 1 Publication	1
Mutagenesisⁱ	403	D → A: No loss of activity. 1 Publication	1
Mutagenesisⁱ	439	E → A: No loss of activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000057898	1 – 578	Isocitrate dehydrogenase kinase/phosphataseAdd BLAST		578

Proteomic databases

PaxDbⁱ	P11071
PRIDEⁱ	P11071

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
icd	P08200	5	EBI-1112800,EBI-369069

With

Entry

#Exp.

IntAct

Notes

icd

P08200

5

EBI-1112800,EBI-369069

Protein-protein interaction databases

BioGridⁱ	4261467, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-9041N
IntActⁱ	P11071, 12 interactors
STRINGⁱ	316385.ECDH10B_4205

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P11071
SMRⁱ	P11071
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the AceK family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105VS7 Bacteria COG4579 LUCA
HOGENOMⁱ	HOG000247673
InParanoidⁱ	P11071
KEGG Orthology (KO) More...KOⁱ	K00906

Family and domain databases

HAMAPⁱ	MF_00747 AceK, 1 hit
InterProⁱ	View protein in InterPro IPR010452 Isocitrate_DH_AceK
PANTHERⁱ	PTHR39559 PTHR39559, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06315 AceK, 1 hit
PIRSFⁱ	PIRSF000719 AceK, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD043552 Isocitrate_DH_AceK, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P11071-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR 
        60         70         80         90        100
IHLYDHHVGL VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE 
       110        120        130        140        150
SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE 
       160        170        180        190        200
SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL 
       210        220        230        240        250
FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF 
       260        270        280        290        300
GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 
       310        320        330        340        350
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA 
       360        370        380        390        400
AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK 
       410        420        430        440        450
ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA 
       460        470        480        490        500
ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL 
       510        520        530        540        550
ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN 
       560        570 
RIREGHVEDV YAYRRRQRFS VRYGEMLF

Length:578

Mass (Da):67,699

Last modified:December 1, 2000 - v2

Checksum:ⁱ5ABD5FB5FA1D9B29

GO

Sequence cautionⁱ

The sequence AAA24007 differs from that shown. Reason: Frameshift at positions 92, 137 and 166.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	140	A → G in AAA24007 (PubMed:2826408).Curated		1
Sequence conflictⁱ	416 – 417	ER → DG in AAA24010 (PubMed:2836370).Curated		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M20714 Genomic DNA Translation: AAA24010.1 M18974 Genomic DNA Translation: AAA24007.1 Frameshift. U00006 Genomic DNA Translation: AAC43110.1 U00096 Genomic DNA Translation: AAC76986.1 AP009048 Genomic DNA Translation: BAE78018.1 M22621 Genomic DNA Translation: AAC13651.1 M63497 Genomic DNA Translation: AAA73005.1
PIRⁱ	G65208 KIECID
NCBI Reference Sequences More...RefSeqⁱ	NP_418440.1, NC_000913.3 WP_001137220.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76986; AAC76986; b4016 BAE78018; BAE78018; BAE78018
GeneIDⁱ	944797
KEGGⁱ	ecj:JW3976 eco:b4016
PATRICⁱ	fig\|1411691.4.peg.2697

Protein	Similar proteins		Species	Score	Length	Source
P11071	Isocitrate dehydrogenase kinase/phosphatase		ECOBW		578	UniRef100_C5A0V3
Isocitrate dehydrogenase kinase/phosphatase		ECODH		578
Bifunctional isocitrate dehydrogenase kinase/phosphatase		Shigella sp.		578
Isocitrate dehydrogenase kinase/phosphatase		ECOLX		578
Isocitrate dehydrogenase kinase/phosphatase		SHIBO		578
+27

Protein	Similar proteins		Species	Score	Length	Source
P11071	Isocitrate dehydrogenase kinase/phosphatase	)	ECO57		578	UniRef90_Q8X607
Isocitrate dehydrogenase kinase/phosphatase		ECO5E		578
Isocitrate dehydrogenase kinase/phosphatase		SALTI		593
Isocitrate dehydrogenase kinase/phosphatase		SALA4		583
Isocitrate dehydrogenase kinase/phosphatase		SALNS		583
+1226

Protein	Similar proteins		Species	Score	Length	Source
P11071	Isocitrate dehydrogenase kinase/phosphatase		PECAS		591	UniRef50_Q6D012
Isocitrate dehydrogenase kinase/phosphatase		PECCP		591
Isocitrate dehydrogenase kinase/phosphatase		AERS4		575
Isocitrate dehydrogenase kinase/phosphatase		AERHH		575
Isocitrate dehydrogenase kinase/phosphatase		YERPS		575
+2791

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M20714 Genomic DNA Translation: AAA24010.1 M18974 Genomic DNA Translation: AAA24007.1 Frameshift. U00006 Genomic DNA Translation: AAC43110.1 U00096 Genomic DNA Translation: AAC76986.1 AP009048 Genomic DNA Translation: BAE78018.1 M22621 Genomic DNA Translation: AAC13651.1 M63497 Genomic DNA Translation: AAA73005.1
PIRⁱ	G65208 KIECID
RefSeqⁱ	NP_418440.1, NC_000913.3 WP_001137220.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P11071
SMRⁱ	P11071
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261467, 11 interactors
DIPⁱ	DIP-9041N
IntActⁱ	P11071, 12 interactors
STRINGⁱ	316385.ECDH10B_4205

Proteomic databases

PaxDbⁱ	P11071
PRIDEⁱ	P11071

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76986; AAC76986; b4016 BAE78018; BAE78018; BAE78018
GeneIDⁱ	944797
KEGGⁱ	ecj:JW3976 eco:b4016
PATRICⁱ	fig\|1411691.4.peg.2697

Organism-specific databases

EchoBASEⁱ	EB0025
EcoGeneⁱ	EG10026 aceK

Phylogenomic databases

eggNOGⁱ	ENOG4105VS7 Bacteria COG4579 LUCA
HOGENOMⁱ	HOG000247673
InParanoidⁱ	P11071
KOⁱ	K00906

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ICITDEHASE-KIN-PHOSPHA MetaCyc:ICITDEHASE-KIN-PHOSPHA
BRENDAⁱ	2.7.11.5 2026
SABIO-RKⁱ	P11071

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P11071

PROⁱ

PR:P11071

Family and domain databases

HAMAPⁱ	MF_00747 AceK, 1 hit
InterProⁱ	View protein in InterPro IPR010452 Isocitrate_DH_AceK
PANTHERⁱ	PTHR39559 PTHR39559, 1 hit
Pfamⁱ	View protein in Pfam PF06315 AceK, 1 hit
PIRSFⁱ	PIRSF000719 AceK, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD043552 Isocitrate_DH_AceK, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACEK_ECOLI
Accessionⁱ	P11071Primary (citable) accession number: P11071 Secondary accession number(s): P11070, Q2M6T8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	December 1, 2000
	Last modified:	November 7, 2018
	This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P11071 (ACEK_ECOLI)

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Isocitrate dehydrogenase kinase/phosphatase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ