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UniProtKB - P11045 (DYR_BACSU)
Protein
Dihydrofolate reductase
Gene
dfrA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).
By similarityCatalytic activityi
- EC:1.5.1.3PROSITE-ProRule annotation
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate. This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | SubstrateBy similarity | 1 | |
Binding sitei | 57 | SubstrateBy similarity | 1 | |
Binding sitei | 114 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 7 | NADPBy similarity | 2 | |
Nucleotide bindingi | 14 – 19 | NADPBy similarity | 6 | |
Nucleotide bindingi | 43 – 46 | NADPBy similarity | 4 | |
Nucleotide bindingi | 62 – 65 | NADPBy similarity | 4 | |
Nucleotide bindingi | 95 – 100 | NADPBy similarity | 6 |
GO - Molecular functioni
- dihydrofolate reductase activity Source: GO_Central
- NADP binding Source: GO_Central
GO - Biological processi
- dihydrofolate metabolic process Source: GO_Central
- folic acid metabolic process Source: GO_Central
- glycine biosynthetic process Source: InterPro
- one-carbon metabolic process Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | One-carbon metabolism |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | BSUB:BSU21810-MONOMER |
UniPathwayi | UPA00077;UER00158 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dfrA Ordered Locus Names:BSU21810 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186382 | 1 – 168 | Dihydrofolate reductaseAdd BLAST | 168 |
Proteomic databases
PaxDbi | P11045 |
PRIDEi | P11045 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 159 | DHFRPROSITE-ProRule annotationAdd BLAST | 159 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 5 – 7 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the dihydrofolate reductase family.Curated
Phylogenomic databases
eggNOGi | COG0262, Bacteria |
InParanoidi | P11045 |
OMAi | EECFVIG |
PhylomeDBi | P11045 |
Family and domain databases
CDDi | cd00209, DHFR, 1 hit |
Gene3Di | 3.40.430.10, 1 hit |
InterProi | View protein in InterPro IPR012259, DHFR IPR024072, DHFR-like_dom_sf IPR017925, DHFR_CS IPR001796, DHFR_dom |
PANTHERi | PTHR48069, PTHR48069, 1 hit |
Pfami | View protein in Pfam PF00186, DHFR_1, 1 hit |
PIRSFi | PIRSF000194, DHFR, 1 hit |
SUPFAMi | SSF53597, SSF53597, 1 hit |
PROSITEi | View protein in PROSITE PS00075, DHFR_1, 1 hit PS51330, DHFR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P11045-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISFIFAMDA NRLIGKDNDL PWHLPNDLAY FKKITSGHSI IMGRKTFESI
60 70 80 90 100
GRPLPNRKNI VVTSAPDSEF QGCTVVSSLK DVLDICSGPE ECFVIGGAQL
110 120 130 140 150
YTDLFPYADR LYMTKIHHEF EGDRHFPEFD ESNWKLVSSE QGTKDEKNPY
160
DYEFLMYEKK NSSKAGGF
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 165 | A → V in strain: MI112. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20012 Genomic DNA Translation: AAA22853.1 L77246 Genomic DNA Translation: AAA96635.1 AL009126 Genomic DNA Translation: CAB14099.1 |
PIRi | JT0291, RDBSD |
RefSeqi | NP_390064.1, NC_000964.3 WP_003230799.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14099; CAB14099; BSU_21810 |
GeneIDi | 939091 |
KEGGi | bsu:BSU21810 |
PATRICi | fig|224308.179.peg.2383 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20012 Genomic DNA Translation: AAA22853.1 L77246 Genomic DNA Translation: AAA96635.1 AL009126 Genomic DNA Translation: CAB14099.1 |
PIRi | JT0291, RDBSD |
RefSeqi | NP_390064.1, NC_000964.3 WP_003230799.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P11045 |
SMRi | P11045 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU21810 |
Proteomic databases
PaxDbi | P11045 |
PRIDEi | P11045 |
Genome annotation databases
EnsemblBacteriai | CAB14099; CAB14099; BSU_21810 |
GeneIDi | 939091 |
KEGGi | bsu:BSU21810 |
PATRICi | fig|224308.179.peg.2383 |
Phylogenomic databases
eggNOGi | COG0262, Bacteria |
InParanoidi | P11045 |
OMAi | EECFVIG |
PhylomeDBi | P11045 |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00158 |
BioCyci | BSUB:BSU21810-MONOMER |
Family and domain databases
CDDi | cd00209, DHFR, 1 hit |
Gene3Di | 3.40.430.10, 1 hit |
InterProi | View protein in InterPro IPR012259, DHFR IPR024072, DHFR-like_dom_sf IPR017925, DHFR_CS IPR001796, DHFR_dom |
PANTHERi | PTHR48069, PTHR48069, 1 hit |
Pfami | View protein in Pfam PF00186, DHFR_1, 1 hit |
PIRSFi | PIRSF000194, DHFR, 1 hit |
SUPFAMi | SSF53597, SSF53597, 1 hit |
PROSITEi | View protein in PROSITE PS00075, DHFR_1, 1 hit PS51330, DHFR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DYR_BACSU | |
Accessioni | P11045Primary (citable) accession number: P11045 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families