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Protein

Thymidylate synthase 2

Gene

thyA2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Miscellaneous

B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Temperature dependencei

Thermolabile. Inactive at 46 degrees Celsius.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei146NucleophileUniRule annotation1
Binding sitei1695,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei177dUMPUniRule annotation1
Binding sitei2635,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi166 – 169dUMPUniRule annotation4
Nucleotide bindingi207 – 209dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU21820-MONOMER
SABIO-RKiP11044
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 2UniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TS 2UniRule annotation
Short name:
TSase 2UniRule annotation
Alternative name(s):
Thymidylate synthase A1 Publication
Short name:
TS A1 Publication
Short name:
TSase A1 Publication
Gene namesi
Name:thyA2UniRule annotation
Synonyms:thyB
Ordered Locus Names:BSU21820
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409321 – 264Thymidylate synthase 2Add BLAST264

Proteomic databases

PaxDbiP11044
PRIDEiP11044

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP11044, 6 interactors
STRINGi224308.Bsubs1_010100012016

Structurei

3D structure databases

ProteinModelPortaliP11044
SMRiP11044
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
InParanoidiP11044
KOiK00560
OMAiKQYLDLC
PhylomeDBiP11044

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P11044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYKDFCRH VLEHGEKKGD RTGTGTISTF GYQMRFNLRE GFPMLTTKKL
60 70 80 90 100
HFKSIAHELL WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS
110 120 130 140 150
WRGADGETID QISRLIEDIK TNPNSRRLIV SAWNVGEIDK MALPPCHCLF
160 170 180 190 200
QFYVSDGKLS CQLYQRSADV FLGVPFNIAS YALLTMIIAH VTGLEPGEFI
210 220 230 240 250
HTFGDVHIYQ NHIEQVNLQL ERDVRPLPQL RFARKVDSIF NFAFEDFIIE
260
DYDPHPHIKG AVSV
Length:264
Mass (Da):30,538
Last modified:July 1, 1989 - v1
Checksum:i1BFE1F1BD8202E64
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti7F → L in strain: ATCC 6633. 1
Natural varianti37N → H in strain: ATCC 6633. 1
Natural varianti39R → Q in strain: ATCC 6633. 1
Natural varianti187I → M in strain: ATCC 6633. 1
Natural varianti221E → T in strain: ATCC 6633. 1
Natural varianti224V → L in strain: ATCC 6633. 1
Natural varianti229Q → K in strain: ATCC 6633. 1
Natural varianti235 – 236KV → EI in strain: ATCC 6633. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69661 Genomic DNA Translation: CAA49350.1
L77246 Genomic DNA Translation: AAA96634.1
M20012 Genomic DNA Translation: AAA22852.1
AL009126 Genomic DNA Translation: CAB14100.1
PIRiJT0290 SYBSTB
S35239
RefSeqiNP_390065.1, NC_000964.3
WP_004398587.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14100; CAB14100; BSU21820
GeneIDi939092
KEGGibsu:BSU21820
PATRICifig|224308.179.peg.2384

Similar proteinsi

Entry informationi

Entry nameiTYSY2_BACSU
AccessioniPrimary (citable) accession number: P11044
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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