UniProtKB - P10980 (ACM2_RAT)
Muscarinic acetylcholine receptor M2
Chrm2
Functioni
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).
By similarityGO - Molecular functioni
- arrestin family protein binding Source: RGD
- G protein-coupled acetylcholine receptor activity Source: RGD
- G protein-coupled serotonin receptor activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
- chemical synaptic transmission Source: GO_Central
- G protein-coupled acetylcholine receptor signaling pathway Source: RGD
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- regulation of heart contraction Source: InterPro
- regulation of smooth muscle contraction Source: RGD
- regulation of synaptic vesicle exocytosis Source: RGD
- response to virus Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-418594, G alpha (i) signalling events R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Muscarinic acetylcholine receptor M2 |
Gene namesi | Name:Chrm2 Synonyms:Chrm-2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620023, Chrm2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
- postsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Phosphorylation in response to agonist binding promotes receptor internalization.By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of postsynaptic membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: RGD
Other locations
- asymmetric synapse Source: RGD
- axon terminus Source: RGD
- cholinergic synapse Source: RGD
- dendrite Source: RGD
- glutamatergic synapse Source: SynGO
- integral component of membrane Source: RGD
- membrane Source: RGD
- neuronal cell body Source: RGD
- symmetric synapse Source: RGD
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 23 – 45 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 46 – 59 | CytoplasmicBy similarityAdd BLAST | 14 | |
Transmembranei | 60 – 80 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 81 – 97 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 98 – 119 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 120 – 139 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 140 – 162 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 163 – 184 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 185 – 209 | Helical; Name=5By similarityAdd BLAST | 25 | |
Topological domaini | 210 – 387 | CytoplasmicBy similarityAdd BLAST | 178 | |
Transmembranei | 388 – 410 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 411 – 418 | ExtracellularBy similarity | 8 | |
Transmembranei | 419 – 442 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 443 – 466 | CytoplasmicBy similarityAdd BLAST | 24 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069025 | 1 – 466 | Muscarinic acetylcholine receptor M2Add BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 3 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 96 ↔ 176 | PROSITE-ProRule annotation | ||
Modified residuei | 232 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 413 ↔ 416 | PROSITE-ProRule annotation | ||
Modified residuei | 446 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 450 | PhosphothreonineSequence analysis | 1 | |
Modified residuei | 465 | PhosphothreonineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P10980 |
PTM databases
GlyGeni | P10980, 3 sites |
iPTMneti | P10980 |
PhosphoSitePlusi | P10980 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000046972, Expressed in heart and 15 other tissues |
Interactioni
Subunit structurei
Interacts with ARRB1 and ARRB2.
Interacts with RACK1; the interaction regulates CHRM2 internalization (By similarity).
By similarityGO - Molecular functioni
- arrestin family protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000015864 |
Chemistry databases
BindingDBi | P10980 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 103 – 107 | Agonist bindingBy similarity | 5 | |
Regioni | 218 – 289 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 403 – 404 | Agonist bindingBy similarity | 2 | |
Regioni | 426 – 430 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 120 – 122 | Important for signaling | 3 | |
Motifi | 436 – 440 | Important for signaling | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 230 – 244 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4220, Eukaryota |
GeneTreei | ENSGT00940000158940 |
HOGENOMi | CLU_009579_11_2_1 |
InParanoidi | P10980 |
OMAi | FTSWNAS |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P10980 |
TreeFami | TF320495 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001065, Musac_Ach_M2_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00539, MUSCRINICM2R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSRDDNS KQTCIKIVTK AQKGDVCTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | N → S in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 273 | N → T in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 289 – 290 | VS → SA in AAA40926 (PubMed:2825184).Curated | 2 | |
Sequence conflicti | 313 | G → D in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 337 | C → Y in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 353 | N → S in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 360 | I → V in AAA40926 (PubMed:2825184).Curated | 1 | |
Sequence conflicti | 369 | T → P in AAA40926 (PubMed:2825184).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03025 mRNA Translation: AAA40926.1 AB017655 mRNA Translation: BAA36838.1 |
PIRi | JH0197 S10856 |
RefSeqi | NP_112278.1, NM_031016.1 |
Genome annotation databases
Ensembli | ENSRNOT00000075341; ENSRNOP00000065178; ENSRNOG00000046972 |
GeneIDi | 81645 |
KEGGi | rno:81645 |
UCSCi | RGD:620023, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03025 mRNA Translation: AAA40926.1 AB017655 mRNA Translation: BAA36838.1 |
PIRi | JH0197 S10856 |
RefSeqi | NP_112278.1, NM_031016.1 |
3D structure databases
SMRi | P10980 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000015864 |
Chemistry databases
BindingDBi | P10980 |
ChEMBLi | CHEMBL309 |
DrugCentrali | P10980 |
GuidetoPHARMACOLOGYi | 14 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P10980, 3 sites |
iPTMneti | P10980 |
PhosphoSitePlusi | P10980 |
Proteomic databases
PaxDbi | P10980 |
Genome annotation databases
Ensembli | ENSRNOT00000075341; ENSRNOP00000065178; ENSRNOG00000046972 |
GeneIDi | 81645 |
KEGGi | rno:81645 |
UCSCi | RGD:620023, rat |
Organism-specific databases
CTDi | 1129 |
RGDi | 620023, Chrm2 |
Phylogenomic databases
eggNOGi | KOG4220, Eukaryota |
GeneTreei | ENSGT00940000158940 |
HOGENOMi | CLU_009579_11_2_1 |
InParanoidi | P10980 |
OMAi | FTSWNAS |
OrthoDBi | 1245472at2759 |
PhylomeDBi | P10980 |
TreeFami | TF320495 |
Enzyme and pathway databases
Reactomei | R-RNO-390648, Muscarinic acetylcholine receptors R-RNO-418594, G alpha (i) signalling events R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:P10980 |
Gene expression databases
Bgeei | ENSRNOG00000046972, Expressed in heart and 15 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001065, Musac_Ach_M2_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00539, MUSCRINICM2R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACM2_RAT | |
Accessioni | P10980Primary (citable) accession number: P10980 Secondary accession number(s): Q9Z2Z1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families