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Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390648 Muscarinic acetylcholine receptors
R-RNO-418594 G alpha (i) signalling events
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:Chrm2
Synonyms:Chrm-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620023 Chrm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22ExtracellularBy similarityAdd BLAST22
Transmembranei23 – 45Helical; Name=1By similarityAdd BLAST23
Topological domaini46 – 59CytoplasmicBy similarityAdd BLAST14
Transmembranei60 – 80Helical; Name=2By similarityAdd BLAST21
Topological domaini81 – 97ExtracellularBy similarityAdd BLAST17
Transmembranei98 – 119Helical; Name=3By similarityAdd BLAST22
Topological domaini120 – 139CytoplasmicBy similarityAdd BLAST20
Transmembranei140 – 162Helical; Name=4By similarityAdd BLAST23
Topological domaini163 – 184ExtracellularBy similarityAdd BLAST22
Transmembranei185 – 209Helical; Name=5By similarityAdd BLAST25
Topological domaini210 – 387CytoplasmicBy similarityAdd BLAST178
Transmembranei388 – 410Helical; Name=6By similarityAdd BLAST23
Topological domaini411 – 418ExtracellularBy similarity8
Transmembranei419 – 442Helical; Name=7By similarityAdd BLAST24
Topological domaini443 – 466CytoplasmicBy similarityAdd BLAST24

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL309
GuidetoPHARMACOLOGYi14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690251 – 466Muscarinic acetylcholine receptor M2Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi3N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Modified residuei232PhosphoserineCombined sources1
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446PhosphothreonineSequence analysis1
Modified residuei450PhosphothreonineSequence analysis1
Modified residuei465PhosphothreonineSequence analysis1

Post-translational modificationi

Phosphorylated in response to agonist treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP10980

PTM databases

iPTMnetiP10980
PhosphoSitePlusiP10980

Expressioni

Gene expression databases

BgeeiENSRNOG00000046972 Expressed in 6 organ(s), highest expression level in heart

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2. Interacts with RACK1; the interaction regulates CHRM2 internalization (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065178

Chemistry databases

BindingDBiP10980

Structurei

3D structure databases

ProteinModelPortaliP10980
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 107Agonist bindingBy similarity5
Regioni403 – 404Agonist bindingBy similarity2
Regioni426 – 430Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi120 – 122Important for signaling3
Motifi436 – 440Important for signaling5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP10980
KOiK04130
OMAiCIKIVTK
OrthoDBiEOG091G06VI
PhylomeDBiP10980
TreeFamiTF320495

Family and domain databases

CDDicd15297 7tmA_mAChR_M2, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001065 Musac_Ach_M2_rcpt
IPR000995 Musac_Ach_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00539 MUSCRINICM2R
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P10980-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSRDDNS KQTCIKIVTK AQKGDVCTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,539
Last modified:December 1, 2000 - v2
Checksum:i70ECCD86366A676B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51N → S in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti273N → T in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti289 – 290VS → SA in AAA40926 (PubMed:2825184).Curated2
Sequence conflicti313G → D in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti337C → Y in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti353N → S in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti360I → V in AAA40926 (PubMed:2825184).Curated1
Sequence conflicti369T → P in AAA40926 (PubMed:2825184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03025 mRNA Translation: AAA40926.1
AB017655 mRNA Translation: BAA36838.1
PIRiJH0197
S10856
RefSeqiNP_112278.1, NM_031016.1
UniGeneiRn.10752
Rn.200974

Genome annotation databases

EnsembliENSRNOT00000075341; ENSRNOP00000065178; ENSRNOG00000046972
GeneIDi81645
KEGGirno:81645
UCSCiRGD:620023 rat

Similar proteinsi

Entry informationi

Entry nameiACM2_RAT
AccessioniPrimary (citable) accession number: P10980
Secondary accession number(s): Q9Z2Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 1, 2000
Last modified: September 12, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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