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Protein

Acetylserotonin O-methyltransferase

Gene

ASMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).By similarity

Catalytic activityi

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serotonin N-acetyltransferase (AANAT), Acetylserotonin O-methyltransferase (ASMT)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei164S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei210S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei252S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei255Proton donor/acceptorBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei306SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processMelatonin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayi
UPA00837;UER00815

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylserotonin O-methyltransferase (EC:2.1.1.4)
Alternative name(s):
Hydroxyindole O-methyltransferase
Short name:
HIOMT
Gene namesi
Name:ASMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243914

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839801 – 345Acetylserotonin O-methyltransferaseAdd BLAST345

Proteomic databases

PaxDbiP10950
PRIDEiP10950

Expressioni

Tissue specificityi

Expressed in the pineal gland (at protein level). Not detectable in retina, nor in liver.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000054648

Structurei

3D structure databases

ProteinModelPortaliP10950
SMRiP10950
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237S-adenosyl-L-methionine bindingPROSITE-ProRule annotation3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3178 Eukaryota
ENOG410XS7T LUCA
HOVERGENiHBG001526
InParanoidiP10950
KOiK00543

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR031725 ASMT_dimerisation
IPR016461 O-MeTrfase_COMT
IPR001077 O_MeTrfase_2
IPR029063 SAM-dependent_MTases
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF16864 Dimerisation2, 1 hit
PF00891 Methyltransf_2, 1 hit
PIRSFiPIRSF005739 O-mtase, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51683 SAM_OMT_II, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P10950-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCSQEGEGYS LLKEYANGFM VSQVLFAACE LGVFELLAEA LEPLDSAAVS
60 70 80 90 100
SHLGSSPQGT ELLLNTCVSL KLLQADVRGG KAVYANTELA STYLVRGSPR
110 120 130 140 150
SQRDMLLYAG RTAYVCWRHL AEAVREGRNQ YLKAFGIPSE ELFSAIYRSE
160 170 180 190 200
DERLQFMQGL QDVWRLEGAT VLAAFDLSPF PLICDLGGGS GALAKACVSL
210 220 230 240 250
YPGCRAIVFD IPGVVQIAKR HFSASEDERI SFHEGDFFKD ALPEADLYIL
260 270 280 290 300
ARVLHDWTDA KCSHLLQRVY RACRTGGGIL VIESLLDTDG RGPLTTLLYS
310 320 330 340
LNMLVQTEGR ERTPAEYRAL LGPAGFRDVR CRRTGGTYDA VLARK
Length:345
Mass (Da):37,924
Last modified:November 1, 1997 - v2
Checksum:iAA8EB84EB8D4A7E4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H9GW41H9GW41_BOVIN
Acetylserotonin O-methyltransferase
ASMT
209Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18G → A in AAA30565 (PubMed:3818627).Curated1
Sequence conflicti58 – 121QGTEL…WRHLA → GDRAATEHLCVPEAAASRRE GRKSCVCKHGARQHLPGERQ PQVPAGHAAVRGQDRLRLLA PPG in AAA30565 (PubMed:3818627).CuratedAdd BLAST64
Sequence conflicti315 – 345AEYRA…VLARK → GRSTARSVGPAASETCGDGG RGEPTMLSWPGNQACSV in AAA30565 (PubMed:3818627).CuratedAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02671 mRNA Translation: AAA30565.1
M81862 mRNA Translation: AAA30570.1
PIRiA42106
RefSeqiNP_803459.1, NM_177493.2
UniGeneiBt.3930

Genome annotation databases

GeneIDi281013
KEGGibta:281013

Similar proteinsi

Entry informationi

Entry nameiASMT_BOVIN
AccessioniPrimary (citable) accession number: P10950
Secondary accession number(s): Q28123
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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