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Protein

Histidine ammonia-lyase

Gene

hutH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-histidine = urocanate + NH3.

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHistidine metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU39350-MONOMER
MetaCyc:MONOMER-11609
UniPathwayi
UPA00379;UER00549

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine ammonia-lyase (EC:4.3.1.3)
Short name:
Histidase
Gene namesi
Name:hutH
Ordered Locus Names:BSU39350
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001609911 – 508Histidine ammonia-lyaseAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki141 ↔ 1435-imidazolinone (Ala-Gly)By similarity
Modified residuei1422,3-didehydroalanine (Ser)By similarity1

Post-translational modificationi

Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.By similarity

Proteomic databases

PaxDbiP10944
PRIDEiP10944

Expressioni

Inductioni

By histidine.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021236

Structurei

3D structure databases

ProteinModelPortaliP10944
SMRiP10944
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAL/histidase family.Curated

Phylogenomic databases

eggNOGiENOG4105C84 Bacteria
COG2986 LUCA
HOGENOMiHOG000237619
InParanoidiP10944
KOiK01745
OMAiCAPQVAG
PhylomeDBiP10944

Family and domain databases

CDDicd00332 PAL-HAL, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00229 His_ammonia_lyase, 1 hit
InterProiView protein in InterPro
IPR001106 Aromatic_Lyase
IPR024083 Fumarase/histidase_N
IPR005921 HutH
IPR008948 L-Aspartase-like
IPR022313 Phe/His_NH3-lyase_AS
PANTHERiPTHR10362 PTHR10362, 1 hit
PfamiView protein in Pfam
PF00221 Lyase_aromatic, 1 hit
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR01225 hutH, 1 hit
PROSITEiView protein in PROSITE
PS00488 PAL_HISTIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

P10944-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTLDGSSLT TADVARVLFD FEEAAASEES MERVKKSRAA VERIVRDEKT
60 70 80 90 100
IYGINTGFGK FSDVLIQKED SAALQLNLIL SHACGVGDPF PECVSRAMLL
110 120 130 140 150
LRANALLKGF SGVRAELIEQ LLAFLNKRVH PVIPQQGSLG ASGDLAPLSH
160 170 180 190 200
LALALIGQGE VFFEGERMPA MTGLKKAGIQ PVTLTSKEGL ALINGTQAMT
210 220 230 240 250
AMGVVAYIEA EKLAYQTERI ASLTIEGLQG IIDAFDEDIH LARGYQEQID
260 270 280 290 300
VAERIRFYLS DSGLTTSQGE LRVQDAYSLR CIPQVHGATW QTLGYVKEKL
310 320 330 340 350
EIEMNAATDN PLIFNDGDKV ISGGNFHGQP IAFAMDFLKI AISELANIAE
360 370 380 390 400
RRIERLVNPQ LNDLPPFLSP HPGLQSGAMI MQYAAASLVS ENKTLAHPAS
410 420 430 440 450
VDSIPSSANQ EDHVSMGTIA ARHAYQVIAN TRRVIAIEAI CALQAVEYRG
460 470 480 490 500
IEHAASYTKQ LFQEMRKVVP SIQQDRVFSY DIERLTDWLK KESLIPDHQN

KELRGMNI
Length:508
Mass (Da):55,675
Last modified:July 1, 1989 - v1
Checksum:i869C323BFCC318E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20659 Genomic DNA Translation: AAA22538.1
D31856 Genomic DNA Translation: BAA06644.1
AL009126 Genomic DNA Translation: CAB15971.1
PIRiS18810 UFBSHS
RefSeqiNP_391814.1, NC_000964.3
WP_003243255.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15971; CAB15971; BSU39350
GeneIDi937541
KEGGibsu:BSU39350
PATRICifig|224308.179.peg.4260

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20659 Genomic DNA Translation: AAA22538.1
D31856 Genomic DNA Translation: BAA06644.1
AL009126 Genomic DNA Translation: CAB15971.1
PIRiS18810 UFBSHS
RefSeqiNP_391814.1, NC_000964.3
WP_003243255.1, NZ_JNCM01000034.1

3D structure databases

ProteinModelPortaliP10944
SMRiP10944
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021236

Proteomic databases

PaxDbiP10944
PRIDEiP10944

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15971; CAB15971; BSU39350
GeneIDi937541
KEGGibsu:BSU39350
PATRICifig|224308.179.peg.4260

Phylogenomic databases

eggNOGiENOG4105C84 Bacteria
COG2986 LUCA
HOGENOMiHOG000237619
InParanoidiP10944
KOiK01745
OMAiCAPQVAG
PhylomeDBiP10944

Enzyme and pathway databases

UniPathwayi
UPA00379;UER00549

BioCyciBSUB:BSU39350-MONOMER
MetaCyc:MONOMER-11609

Family and domain databases

CDDicd00332 PAL-HAL, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00229 His_ammonia_lyase, 1 hit
InterProiView protein in InterPro
IPR001106 Aromatic_Lyase
IPR024083 Fumarase/histidase_N
IPR005921 HutH
IPR008948 L-Aspartase-like
IPR022313 Phe/His_NH3-lyase_AS
PANTHERiPTHR10362 PTHR10362, 1 hit
PfamiView protein in Pfam
PF00221 Lyase_aromatic, 1 hit
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR01225 hutH, 1 hit
PROSITEiView protein in PROSITE
PS00488 PAL_HISTIDASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHUTH_BACSU
AccessioniPrimary (citable) accession number: P10944
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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