Spp1

Functionⁱ

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

GO - Molecular functionⁱ

cytokine activity
Source: UniProtKB
Non-traceable author statementⁱ
- 11264474
extracellular matrix binding
Source: MGI
Inferred from direct assayⁱ
- 18757743

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

androgen catabolic process Source: MGI
biomineral tissue development Source: UniProtKB-KW
cell adhesion Source: MGI
cellular response to fluid shear stress
Source: MGI
Inferred from direct assayⁱ
- 24841674
cellular response to leukemia inhibitory factor
Source: MGI
Inferred from expression patternⁱ
- 20439489
cellular response to testosterone stimulus Source: MGI
negative regulation of apoptotic process
Source: UniProtKB
Non-traceable author statementⁱ
- 12183442
neutrophil chemotaxis
Source: MGI
Inferred from direct assayⁱ
- 21321126
osteoblast differentiation Source: MGI
positive regulation of bone resorption Source: MGI
positive regulation of cell-substrate adhesion
Source: MGI
Inferred from direct assayⁱ
- 18757743
positive regulation of estradiol secretion Source: MGI
positive regulation of transcription, DNA-templated Source: MGI
response to organic substance Source: MGI
response to steroid hormone Source: MGI
response to vitamin D Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Cytokine
Biological process	Biomineralization, Cell adhesion
Ligand	Sialic acid

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1474228 Degradation of the extracellular matrix R-MMU-186797 Signaling by PDGF R-MMU-216083 Integrin cell surface interactions R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275 Post-translational protein phosphorylation

Reactomeⁱ

R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-186797 Signaling by PDGF
R-MMU-216083 Integrin cell surface interactions
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Osteopontin Alternative name(s): 2AR Bone sialoprotein 1 Calcium oxalate crystal growth inhibitor protein Early T-lymphocyte activation 1 protein Minopontin Secreted phosphoprotein 1 Short name: SPP-1
Gene namesⁱ	Name:Spp1 Synonyms:Eta-1, Op, Spp-1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 5

Organism-specific databases

MGIⁱ	MGI:98389 Spp1

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 16	Sequence analysisAdd BLAST		16
ChainⁱPRO_0000020322	17 – 294	OsteopontinAdd BLAST		278

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	24	PhosphoserineBy similarity	1
Modified residueⁱ	26	PhosphoserineCombined sources	1
Modified residueⁱ	27	PhosphoserineBy similarity	1
Modified residueⁱ	61	PhosphoserineBy similarity	1
Modified residueⁱ	62	PhosphoserineBy similarity	1
Modified residueⁱ	75	PhosphoserineBy similarity	1
Modified residueⁱ	77	PhosphoserineBy similarity	1
Modified residueⁱ	80	PhosphoserineBy similarity	1
Modified residueⁱ	106	PhosphoserineBy similarity	1
Modified residueⁱ	109	PhosphoserineBy similarity	1
Modified residueⁱ	112	PhosphoserineBy similarity	1
Modified residueⁱ	115	PhosphoserineBy similarity	1
Modified residueⁱ	118	PhosphoserineBy similarity	1
Glycosylationⁱ	123	O-linked (GalNAc...) threonineBy similarity	1
Glycosylationⁱ	132	O-linked (GalNAc...) threonineBy similarity	1
Glycosylationⁱ	137	O-linked (GalNAc...) threonineBy similarity	1
Modified residueⁱ	170	PhosphothreonineBy similarity	1
Modified residueⁱ	175	PhosphothreonineBy similarity	1
Modified residueⁱ	176	PhosphoserineBy similarity	1
Modified residueⁱ	180	PhosphoserineBy similarity	1
Modified residueⁱ	200	PhosphoserineBy similarity	1
Modified residueⁱ	209	PhosphoserineBy similarity	1
Modified residueⁱ	213	PhosphoserineBy similarity	1
Modified residueⁱ	219	PhosphoserineBy similarity	1
Modified residueⁱ	222	PhosphothreonineBy similarity	1
Modified residueⁱ	228	PhosphoserineCombined sources	1
Modified residueⁱ	231	PhosphoserineCombined sources	1
Modified residueⁱ	234	PhosphoserineCombined sources	1
Modified residueⁱ	238	PhosphoserineBy similarity	1
Modified residueⁱ	243	PhosphoserineBy similarity	1
Modified residueⁱ	247	PhosphoserineBy similarity	1
Modified residueⁱ	250	PhosphoserineBy similarity	1
Modified residueⁱ	255	PhosphoserineBy similarity	1
Modified residueⁱ	260	PhosphoserineBy similarity	1
Modified residueⁱ	271	PhosphoserineBy similarity	1
Modified residueⁱ	283	PhosphoserineCombined sources	1
Modified residueⁱ	288	PhosphoserineCombined sources	1
Modified residueⁱ	290	PhosphoserineCombined sources	1
Modified residueⁱ	291	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity

O-glycosylated.By similarity

Keywords - PTMⁱ

Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P10923
PaxDbⁱ	P10923
PeptideAtlas More...PeptideAtlasⁱ	P10923
PRIDEⁱ	P10923

PTM databases

iPTMnetⁱ	P10923
PhosphoSitePlusⁱ	P10923

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000029304 Expressed in 279 organ(s), highest expression level in cochlea
CleanExⁱ	MM_SPP1
ExpressionAtlasⁱ	P10923 baseline and differential
Genevisibleⁱ	P10923 MM

Interactionⁱ

Subunit structureⁱ

Ligand for integrin alpha-V/beta-3.

GO - Molecular functionⁱ

cytokine activity
Source: UniProtKB
Non-traceable author statementⁱ
- 11264474

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	203467, 3 interactors
IntActⁱ	P10923, 1 interactor
STRINGⁱ	10090.ENSMUSP00000084043

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P10923
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	144 – 146	Cell attachment site		3

Sequence similaritiesⁱ

Belongs to the osteopontin family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IVP1 Eukaryota ENOG41116B5 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000002509
HOGENOMⁱ	HOG000059656
HOVERGENⁱ	HBG001731
InParanoidⁱ	P10923
KEGG Orthology (KO) More...KOⁱ	K06250
PhylomeDBⁱ	P10923
TreeFamⁱ	TF350201

Family and domain databases

InterProⁱ	View protein in InterPro IPR002038 Osteopontin IPR019841 Osteopontin_CS
PANTHERⁱ	PTHR10607 PTHR10607, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00865 Osteopontin, 1 hit
PRINTSⁱ	PR00216 OSTEOPONTIN
SMARTⁱ	View protein in SMART SM00017 OSTEO, 1 hit
PROSITEⁱ	View protein in PROSITE PS00884 OSTEOPONTIN, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show all Align All

P10923-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK 
        60         70         80         90        100
QNLLAPQNAV SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES 
       110        120        130        140        150
EDSVDSDESD ESHHSDESDE TVTASTQADT FTPIVPTVDV PNGRGDSLAY 
       160        170        180        190        200
GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS GESKESLDVI PVAQLLSMPS 
       210        220        230        240        250
DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV IDSQASSKAS 
       260        270        280        290 
LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN

Length:294

Mass (Da):32,459

Last modified:July 1, 1989 - v1

Checksum:ⁱ9D5F32D67ABC53EA

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8WIP8	F8WIP8_MOUSE	Osteopontin Osteopontin	Spp1	295	Annotation score:
D3Z513	D3Z513_MOUSE	Osteopontin Osteopontin	Spp1	152	Annotation score:
D3Z4N2	D3Z4N2_MOUSE	Osteopontin Osteopontin	Spp1	164	Annotation score:
D3Z116	D3Z116_MOUSE	Osteopontin Osteopontin	Spp1	48	Annotation score:

Sequence cautionⁱ

The sequence AAH14284 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	43	L → P in CAA34276 (PubMed:2787378).Curated	1
Sequence conflictⁱ	99	E → G in AAA57265 (PubMed:2722855).Curated	1
Sequence conflictⁱ	100	S → N in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	122	V → F in CAA32165 (PubMed:2726465).Curated	1
Sequence conflictⁱ	122	V → F in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	142	N → D in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	171	D → Y in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	188	D → N in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	207	K → R in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	224	R → S in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	232	Q → H in AAH14284 (PubMed:15489334).Curated	1
Sequence conflictⁱ	277	Y → H in AAH14284 (PubMed:15489334).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J04806 mRNA Translation: AAA57265.1 X16151 mRNA Translation: CAA34276.1 X13986 mRNA Translation: CAA32165.1 X51834 Genomic DNA Translation: CAA36132.1 BC014284 mRNA Translation: AAH14284.1 Different initiation. BC057858 mRNA Translation: AAH57858.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS19486.1
PIRⁱ	A37818
NCBI Reference Sequences More...RefSeqⁱ	NP_001191162.1, NM_001204233.1 NP_033289.2, NM_009263.3
UniGeneⁱ	Mm.288474

Genome annotation databases

Ensemblⁱ	ENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304 ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304 ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304
GeneIDⁱ	20750
KEGGⁱ	mmu:20750
UCSC genome browser More...UCSCⁱ	uc008yki.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P10923	Osteopontin		MOUSE		294	UniRef100_P10923
Osteopontin (Fragment)		MOUSE		48
UPI0001547B89		MOUSE		310

Protein	Similar proteins		Species	Score	Length	Source
P10923	Osteopontin		MOUSE		294	UniRef90_P10923
Early T-lymphocyte activator-1 (Fragment)		Mus sp.		279
UPI000A31A8E4		MUSCR		295
Osteopontin (Fragment)		MOUSE		48
Osteopontin		MOUSE		295
+9

Protein	Similar proteins		Species	Score	Length	Source
P10923	Osteopontin		RABIT		311	UniRef50_P10923
Osteopontin		RAT		317
Osteopontin		PIG		303
Osteopontin		MOUSE		294
Early T-lymphocyte activator-1 (Fragment)		Mus sp.		279
+141

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J04806 mRNA Translation: AAA57265.1 X16151 mRNA Translation: CAA34276.1 X13986 mRNA Translation: CAA32165.1 X51834 Genomic DNA Translation: CAA36132.1 BC014284 mRNA Translation: AAH14284.1 Different initiation. BC057858 mRNA Translation: AAH57858.1
CCDSⁱ	CCDS19486.1
PIRⁱ	A37818
RefSeqⁱ	NP_001191162.1, NM_001204233.1 NP_033289.2, NM_009263.3
UniGeneⁱ	Mm.288474

3D structure databases

ProteinModelPortalⁱ	P10923
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	203467, 3 interactors
IntActⁱ	P10923, 1 interactor
STRINGⁱ	10090.ENSMUSP00000084043

PTM databases

iPTMnetⁱ	P10923
PhosphoSitePlusⁱ	P10923

Proteomic databases

EPDⁱ	P10923
PaxDbⁱ	P10923
PeptideAtlasⁱ	P10923
PRIDEⁱ	P10923

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304 ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304 ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304
GeneIDⁱ	20750
KEGGⁱ	mmu:20750
UCSCⁱ	uc008yki.2 mouse

Organism-specific databases

CTDⁱ	6696
MGIⁱ	MGI:98389 Spp1

Phylogenomic databases

eggNOGⁱ	ENOG410IVP1 Eukaryota ENOG41116B5 LUCA
GeneTreeⁱ	ENSGT00390000002509
HOGENOMⁱ	HOG000059656
HOVERGENⁱ	HBG001731
InParanoidⁱ	P10923
KOⁱ	K06250
PhylomeDBⁱ	P10923
TreeFamⁱ	TF350201

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1474228 Degradation of the extracellular matrix R-MMU-186797 Signaling by PDGF R-MMU-216083 Integrin cell surface interactions R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275 Post-translational protein phosphorylation

Reactomeⁱ

R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-186797 Signaling by PDGF
R-MMU-216083 Integrin cell surface interactions
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSⁱ	Spp1 mouse
Protein Ontology More...PROⁱ	PR:P10923
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000029304 Expressed in 279 organ(s), highest expression level in cochlea
CleanExⁱ	MM_SPP1
ExpressionAtlasⁱ	P10923 baseline and differential
Genevisibleⁱ	P10923 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR002038 Osteopontin IPR019841 Osteopontin_CS
PANTHERⁱ	PTHR10607 PTHR10607, 1 hit
Pfamⁱ	View protein in Pfam PF00865 Osteopontin, 1 hit
PRINTSⁱ	PR00216 OSTEOPONTIN
SMARTⁱ	View protein in SMART SM00017 OSTEO, 1 hit
PROSITEⁱ	View protein in PROSITE PS00884 OSTEOPONTIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	OSTP_MOUSE
Accessionⁱ	P10923Primary (citable) accession number: P10923 Secondary accession number(s): P19008, Q91VH4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 1, 1989
	Last modified:	November 7, 2018
	This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P10923 (OSTP_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Osteopontin

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Golgi apparatus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ