UniProtKB - P10923 (OSTP_MOUSE)
Osteopontin
Spp1
Functioni
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
By similarityActs as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
2 PublicationsGO - Molecular functioni
- cytokine activity Source: UniProtKB
- extracellular matrix binding Source: MGI
- integrin binding Source: MGI
GO - Biological processi
- androgen catabolic process Source: MGI
- biomineral tissue development Source: UniProtKB-KW
- cell adhesion Source: MGI
- cellular calcium ion homeostasis Source: MGI
- cellular chloride ion homeostasis Source: MGI
- cellular phosphate ion homeostasis Source: MGI
- cellular response to fluid shear stress Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- cellular response to testosterone stimulus Source: MGI
- cellular sodium ion homeostasis Source: MGI
- collecting duct development Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- neutrophil chemotaxis Source: MGI
- osteoblast differentiation Source: MGI
- positive regulation of bone resorption Source: MGI
- positive regulation of cell-substrate adhesion Source: MGI
- positive regulation of estradiol secretion Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- response to organic substance Source: MGI
- response to steroid hormone Source: MGI
- response to vitamin D Source: MGI
- urate biosynthetic process Source: MGI
Keywordsi
Molecular function | Cytokine |
Biological process | Biomineralization, Cell adhesion |
Ligand | Sialic acid |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-186797, Signaling by PDGF R-MMU-216083, Integrin cell surface interactions R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: OsteopontinAlternative name(s): 2AR Bone sialoprotein 1 Calcium oxalate crystal growth inhibitor protein Early T-lymphocyte activation 1 protein Minopontin Secreted phosphoprotein 1 Short name: SPP-1 |
Gene namesi | Name:Spp1 Synonyms:Eta-1, Op, Spp-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98389, Spp1 |
VEuPathDBi | HostDB:ENSMUSG00000029304 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Other locations
- apical part of cell Source: MGI
- cell projection Source: MGI
- cytoplasm Source: MGI
- perinuclear region of cytoplasm Source: MGI
- vesicle Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_0000020322 | 17 – 294 | OsteopontinAdd BLAST | 278 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | PhosphoserineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 | |
Modified residuei | 27 | PhosphoserineBy similarity | 1 | |
Modified residuei | 61 | PhosphoserineBy similarity | 1 | |
Modified residuei | 62 | PhosphoserineBy similarity | 1 | |
Modified residuei | 75 | PhosphoserineBy similarity | 1 | |
Modified residuei | 77 | PhosphoserineBy similarity | 1 | |
Modified residuei | 80 | PhosphoserineBy similarity | 1 | |
Modified residuei | 106 | PhosphoserineBy similarity | 1 | |
Modified residuei | 109 | PhosphoserineBy similarity | 1 | |
Modified residuei | 112 | PhosphoserineBy similarity | 1 | |
Modified residuei | 115 | PhosphoserineBy similarity | 1 | |
Modified residuei | 118 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 123 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Glycosylationi | 132 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Glycosylationi | 137 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Modified residuei | 170 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 175 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 176 | PhosphoserineBy similarity | 1 | |
Modified residuei | 180 | PhosphoserineBy similarity | 1 | |
Modified residuei | 200 | PhosphoserineBy similarity | 1 | |
Modified residuei | 209 | PhosphoserineBy similarity | 1 | |
Modified residuei | 213 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 228 | PhosphoserineCombined sources | 1 | |
Modified residuei | 231 | PhosphoserineCombined sources | 1 | |
Modified residuei | 234 | PhosphoserineCombined sources | 1 | |
Modified residuei | 238 | PhosphoserineBy similarity | 1 | |
Modified residuei | 243 | PhosphoserineBy similarity | 1 | |
Modified residuei | 247 | PhosphoserineBy similarity | 1 | |
Modified residuei | 250 | PhosphoserineBy similarity | 1 | |
Modified residuei | 255 | PhosphoserineBy similarity | 1 | |
Modified residuei | 260 | PhosphoserineBy similarity | 1 | |
Modified residuei | 271 | PhosphoserineBy similarity | 1 | |
Modified residuei | 283 | PhosphoserineCombined sources | 1 | |
Modified residuei | 288 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | PhosphoserineCombined sources | 1 | |
Modified residuei | 291 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3592 |
EPDi | P10923 |
jPOSTi | P10923 |
PaxDbi | P10923 |
PeptideAtlasi | P10923 |
PRIDEi | P10923 |
ProteomicsDBi | 294082 |
PTM databases
GlyGeni | P10923, 3 sites |
iPTMneti | P10923 |
PhosphoSitePlusi | P10923 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029304, Expressed in cochlea and 307 other tissues |
Genevisiblei | P10923, MM |
Interactioni
Subunit structurei
Ligand for integrin alpha-V/beta-3.
GO - Molecular functioni
- cytokine activity Source: UniProtKB
- integrin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203467, 15 interactors |
IntActi | P10923, 1 interactor |
STRINGi | 10090.ENSMUSP00000108367 |
Miscellaneous databases
RNActi | P10923, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 42 – 274 | DisorderedSequence analysisAdd BLAST | 233 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 144 – 146 | Cell attachment site | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 45 – 59 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 62 – 82 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 83 – 106 | Acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 170 – 184 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 197 – 213 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 214 – 236 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 237 – 251 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 252 – 274 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502S5R4, Eukaryota |
GeneTreei | ENSGT00390000002509 |
HOGENOMi | CLU_953033_0_0_1 |
InParanoidi | P10923 |
OrthoDBi | 1280650at2759 |
PhylomeDBi | P10923 |
TreeFami | TF350201 |
Family and domain databases
DisProti | DP01448 |
InterProi | View protein in InterPro IPR002038, Osteopontin IPR019841, Osteopontin_CS |
PANTHERi | PTHR10607, PTHR10607, 1 hit |
Pfami | View protein in Pfam PF00865, Osteopontin, 1 hit |
PRINTSi | PR00216, OSTEOPONTIN |
SMARTi | View protein in SMART SM00017, OSTEO, 1 hit |
PROSITEi | View protein in PROSITE PS00884, OSTEOPONTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK
60 70 80 90 100
QNLLAPQNAV SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES
110 120 130 140 150
EDSVDSDESD ESHHSDESDE TVTASTQADT FTPIVPTVDV PNGRGDSLAY
160 170 180 190 200
GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS GESKESLDVI PVAQLLSMPS
210 220 230 240 250
DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV IDSQASSKAS
260 270 280 290
LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WIP8 | F8WIP8_MOUSE | Osteopontin | Spp1 | 295 | Annotation score: | ||
D3Z4N2 | D3Z4N2_MOUSE | Osteopontin | Spp1 | 164 | Annotation score: | ||
D3Z513 | D3Z513_MOUSE | Osteopontin | Spp1 | 152 | Annotation score: | ||
D3Z116 | D3Z116_MOUSE | Osteopontin | Spp1 | 48 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | L → P in CAA34276 (PubMed:2787378).Curated | 1 | |
Sequence conflicti | 99 | E → G in AAA57265 (PubMed:2722855).Curated | 1 | |
Sequence conflicti | 100 | S → N in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 122 | V → F in CAA32165 (PubMed:2726465).Curated | 1 | |
Sequence conflicti | 122 | V → F in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 142 | N → D in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 171 | D → Y in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 188 | D → N in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 207 | K → R in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 224 | R → S in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 232 | Q → H in AAH14284 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 277 | Y → H in AAH14284 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04806 mRNA Translation: AAA57265.1 X16151 mRNA Translation: CAA34276.1 X13986 mRNA Translation: CAA32165.1 X51834 Genomic DNA Translation: CAA36132.1 BC014284 mRNA Translation: AAH14284.1 Different initiation. BC057858 mRNA Translation: AAH57858.1 |
CCDSi | CCDS19486.1 |
PIRi | A37818 |
RefSeqi | NP_001191162.1, NM_001204233.1 NP_033289.2, NM_009263.3 |
Genome annotation databases
Ensembli | ENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304 ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304 ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304 |
GeneIDi | 20750 |
KEGGi | mmu:20750 |
UCSCi | uc008yki.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04806 mRNA Translation: AAA57265.1 X16151 mRNA Translation: CAA34276.1 X13986 mRNA Translation: CAA32165.1 X51834 Genomic DNA Translation: CAA36132.1 BC014284 mRNA Translation: AAH14284.1 Different initiation. BC057858 mRNA Translation: AAH57858.1 |
CCDSi | CCDS19486.1 |
PIRi | A37818 |
RefSeqi | NP_001191162.1, NM_001204233.1 NP_033289.2, NM_009263.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 203467, 15 interactors |
IntActi | P10923, 1 interactor |
STRINGi | 10090.ENSMUSP00000108367 |
PTM databases
GlyGeni | P10923, 3 sites |
iPTMneti | P10923 |
PhosphoSitePlusi | P10923 |
Proteomic databases
CPTACi | non-CPTAC-3592 |
EPDi | P10923 |
jPOSTi | P10923 |
PaxDbi | P10923 |
PeptideAtlasi | P10923 |
PRIDEi | P10923 |
ProteomicsDBi | 294082 |
Protocols and materials databases
ABCDi | P10923, 7 sequenced antibodies |
Antibodypediai | 3695, 1523 antibodies from 49 providers |
DNASUi | 20750 |
Genome annotation databases
Ensembli | ENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304 ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304 ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304 |
GeneIDi | 20750 |
KEGGi | mmu:20750 |
UCSCi | uc008yki.2, mouse |
Organism-specific databases
CTDi | 6696 |
MGIi | MGI:98389, Spp1 |
VEuPathDBi | HostDB:ENSMUSG00000029304 |
Phylogenomic databases
eggNOGi | ENOG502S5R4, Eukaryota |
GeneTreei | ENSGT00390000002509 |
HOGENOMi | CLU_953033_0_0_1 |
InParanoidi | P10923 |
OrthoDBi | 1280650at2759 |
PhylomeDBi | P10923 |
TreeFami | TF350201 |
Enzyme and pathway databases
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-186797, Signaling by PDGF R-MMU-216083, Integrin cell surface interactions R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 20750, 0 hits in 65 CRISPR screens |
ChiTaRSi | Spp1, mouse |
PROi | PR:P10923 |
RNActi | P10923, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029304, Expressed in cochlea and 307 other tissues |
Genevisiblei | P10923, MM |
Family and domain databases
DisProti | DP01448 |
InterProi | View protein in InterPro IPR002038, Osteopontin IPR019841, Osteopontin_CS |
PANTHERi | PTHR10607, PTHR10607, 1 hit |
Pfami | View protein in Pfam PF00865, Osteopontin, 1 hit |
PRINTSi | PR00216, OSTEOPONTIN |
SMARTi | View protein in SMART SM00017, OSTEO, 1 hit |
PROSITEi | View protein in PROSITE PS00884, OSTEOPONTIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OSTP_MOUSE | |
Accessioni | P10923Primary (citable) accession number: P10923 Secondary accession number(s): P19008, Q91VH4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families