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Protein

Osteopontin

Gene

Spp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

GO - Molecular functioni

  • cytokine activity Source: UniProtKB
  • extracellular matrix binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionCytokine
Biological processBiomineralization, Cell adhesion
LigandSialic acid

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-186797 Signaling by PDGF
R-MMU-216083 Integrin cell surface interactions
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
2AR
Bone sialoprotein 1
Calcium oxalate crystal growth inhibitor protein
Early T-lymphocyte activation 1 protein
Minopontin
Secreted phosphoprotein 1
Short name:
SPP-1
Gene namesi
Name:Spp1
Synonyms:Eta-1, Op, Spp-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:98389 Spp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000002032217 – 294OsteopontinAdd BLAST278

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei27PhosphoserineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei80PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei118PhosphoserineBy similarity1
Glycosylationi123O-linked (GalNAc...) threonineBy similarity1
Glycosylationi132O-linked (GalNAc...) threonineBy similarity1
Glycosylationi137O-linked (GalNAc...) threonineBy similarity1
Modified residuei170PhosphothreonineBy similarity1
Modified residuei175PhosphothreonineBy similarity1
Modified residuei176PhosphoserineBy similarity1
Modified residuei180PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1
Modified residuei228PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei234PhosphoserineCombined sources1
Modified residuei238PhosphoserineBy similarity1
Modified residuei243PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei250PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei260PhosphoserineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei283PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei291PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity
O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP10923
PaxDbiP10923
PeptideAtlasiP10923
PRIDEiP10923

PTM databases

iPTMnetiP10923
PhosphoSitePlusiP10923

Expressioni

Gene expression databases

BgeeiENSMUSG00000029304 Expressed in 279 organ(s), highest expression level in cochlea
CleanExiMM_SPP1
ExpressionAtlasiP10923 baseline and differential
GenevisibleiP10923 MM

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203467, 3 interactors
IntActiP10923, 1 interactor
STRINGi10090.ENSMUSP00000084043

Structurei

3D structure databases

ProteinModelPortaliP10923
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi144 – 146Cell attachment site3

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1 Eukaryota
ENOG41116B5 LUCA
GeneTreeiENSGT00390000002509
HOGENOMiHOG000059656
HOVERGENiHBG001731
InParanoidiP10923
KOiK06250
PhylomeDBiP10923
TreeFamiTF350201

Family and domain databases

InterProiView protein in InterPro
IPR002038 Osteopontin
IPR019841 Osteopontin_CS
PANTHERiPTHR10607 PTHR10607, 1 hit
PfamiView protein in Pfam
PF00865 Osteopontin, 1 hit
PRINTSiPR00216 OSTEOPONTIN
SMARTiView protein in SMART
SM00017 OSTEO, 1 hit
PROSITEiView protein in PROSITE
PS00884 OSTEOPONTIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P10923-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLAVICFCL FGIASSLPVK VTDSGSSEEK LYSLHPDPIA TWLVPDPSQK
60 70 80 90 100
QNLLAPQNAV SSEEKDDFKQ ETLPSNSNES HDHMDDDDDD DDDDGDHAES
110 120 130 140 150
EDSVDSDESD ESHHSDESDE TVTASTQADT FTPIVPTVDV PNGRGDSLAY
160 170 180 190 200
GLRSKSRSFQ VSDEQYPDAT DEDLTSHMKS GESKESLDVI PVAQLLSMPS
210 220 230 240 250
DQDNNGKGSH ESSQLDEPSL ETHRLEHSKE SQESADQSDV IDSQASSKAS
260 270 280 290
LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF RISHELESSS SEVN
Length:294
Mass (Da):32,459
Last modified:July 1, 1989 - v1
Checksum:i9D5F32D67ABC53EA
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WIP8F8WIP8_MOUSE
Osteopontin
Spp1
295Annotation score:
D3Z513D3Z513_MOUSE
Osteopontin
Spp1
152Annotation score:
D3Z4N2D3Z4N2_MOUSE
Osteopontin
Spp1
164Annotation score:
D3Z116D3Z116_MOUSE
Osteopontin
Spp1
48Annotation score:

Sequence cautioni

The sequence AAH14284 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43L → P in CAA34276 (PubMed:2787378).Curated1
Sequence conflicti99E → G in AAA57265 (PubMed:2722855).Curated1
Sequence conflicti100S → N in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti122V → F in CAA32165 (PubMed:2726465).Curated1
Sequence conflicti122V → F in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti142N → D in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti171D → Y in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti188D → N in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti207K → R in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti224R → S in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti232Q → H in AAH14284 (PubMed:15489334).Curated1
Sequence conflicti277Y → H in AAH14284 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04806 mRNA Translation: AAA57265.1
X16151 mRNA Translation: CAA34276.1
X13986 mRNA Translation: CAA32165.1
X51834 Genomic DNA Translation: CAA36132.1
BC014284 mRNA Translation: AAH14284.1 Different initiation.
BC057858 mRNA Translation: AAH57858.1
CCDSiCCDS19486.1
PIRiA37818
RefSeqiNP_001191162.1, NM_001204233.1
NP_033289.2, NM_009263.3
UniGeneiMm.288474

Genome annotation databases

EnsembliENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304
ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304
ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304
GeneIDi20750
KEGGimmu:20750
UCSCiuc008yki.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04806 mRNA Translation: AAA57265.1
X16151 mRNA Translation: CAA34276.1
X13986 mRNA Translation: CAA32165.1
X51834 Genomic DNA Translation: CAA36132.1
BC014284 mRNA Translation: AAH14284.1 Different initiation.
BC057858 mRNA Translation: AAH57858.1
CCDSiCCDS19486.1
PIRiA37818
RefSeqiNP_001191162.1, NM_001204233.1
NP_033289.2, NM_009263.3
UniGeneiMm.288474

3D structure databases

ProteinModelPortaliP10923
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203467, 3 interactors
IntActiP10923, 1 interactor
STRINGi10090.ENSMUSP00000084043

PTM databases

iPTMnetiP10923
PhosphoSitePlusiP10923

Proteomic databases

EPDiP10923
PaxDbiP10923
PeptideAtlasiP10923
PRIDEiP10923

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031243; ENSMUSP00000031243; ENSMUSG00000029304
ENSMUST00000112747; ENSMUSP00000108367; ENSMUSG00000029304
ENSMUST00000112748; ENSMUSP00000108368; ENSMUSG00000029304
GeneIDi20750
KEGGimmu:20750
UCSCiuc008yki.2 mouse

Organism-specific databases

CTDi6696
MGIiMGI:98389 Spp1

Phylogenomic databases

eggNOGiENOG410IVP1 Eukaryota
ENOG41116B5 LUCA
GeneTreeiENSGT00390000002509
HOGENOMiHOG000059656
HOVERGENiHBG001731
InParanoidiP10923
KOiK06250
PhylomeDBiP10923
TreeFamiTF350201

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-186797 Signaling by PDGF
R-MMU-216083 Integrin cell surface interactions
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiSpp1 mouse
PROiPR:P10923
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029304 Expressed in 279 organ(s), highest expression level in cochlea
CleanExiMM_SPP1
ExpressionAtlasiP10923 baseline and differential
GenevisibleiP10923 MM

Family and domain databases

InterProiView protein in InterPro
IPR002038 Osteopontin
IPR019841 Osteopontin_CS
PANTHERiPTHR10607 PTHR10607, 1 hit
PfamiView protein in Pfam
PF00865 Osteopontin, 1 hit
PRINTSiPR00216 OSTEOPONTIN
SMARTiView protein in SMART
SM00017 OSTEO, 1 hit
PROSITEiView protein in PROSITE
PS00884 OSTEOPONTIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOSTP_MOUSE
AccessioniPrimary (citable) accession number: P10923
Secondary accession number(s): P19008, Q91VH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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