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UniProtKB - P10903 (NARK_ECOLI)

Entry version 145 (03 Jul 2019)
Sequence version 2 (01 Jan 1990)
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Protein

Nitrate/nitrite transporter NarK

Gene

narK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. Functions as a nitrate/nitrite exchanger, and protons are probably not co-transported with the substrate.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei89SubstrateCurated1
Binding sitei305SubstrateCurated1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processNitrate assimilation, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:NARK-MONOMER
ECOL316407:JW1214-MONOMER
MetaCyc:NARK-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.1.8.1 the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nitrate/nitrite transporter NarK
Alternative name(s):
Nitrite extrusion protein 1
Nitrite facilitator 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:narK
Ordered Locus Names:b1223, JW1214
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...EcoGenei		EG10642 narK

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 78PeriplasmicSequence analysisAdd BLAST20
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Topological domaini100 – 103CytoplasmicSequence analysis4
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 131PeriplasmicSequence analysis7
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 177CytoplasmicSequence analysisAdd BLAST25
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 211PeriplasmicSequence analysisAdd BLAST13
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 256CytoplasmicSequence analysisAdd BLAST24
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283PeriplasmicSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 319CytoplasmicSequence analysisAdd BLAST15
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 344PeriplasmicSequence analysis4
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 404CytoplasmicSequence analysisAdd BLAST39
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 435PeriplasmicSequence analysis10
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 463CytoplasmicSequence analysis7

GO - Cellular componenti

Complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000967281 – 463Nitrate/nitrite transporter NarKAdd BLAST463

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P10903

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P10903

PRoteomics IDEntifications database

More...PRIDEi		P10903

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Highly expressed during anaerobic growth in the presence of nitrate.2 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		4261633, 21 interactors

STRING: functional protein association networks

More...STRINGi		511145.b1223

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P10903

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG4105DQQ Bacteria
COG2223 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000241722

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P10903

KEGG Orthology (KO)

More...KOi		K02575

Database for complete collections of gene phylogenies

More...PhylomeDBi		P10903

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR011701 MFS
IPR036259 MFS_trans_sf
IPR004737 NO3_transporter

Pfam protein domain database

More...Pfami		View protein in Pfam
PF07690 MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103473 SSF103473, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00886 2A0108, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P10903-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHSSAPERA TGAVITDWRP EDPAFWQQRG QRIASRNLWI SVPCLLLAFC 
        60         70         80         90        100
VWMLFSAVAV NLPKVGFNFT TDQLFMLTAL PSVSGALLRV PYSFMVPIFG 
       110        120        130        140        150
GRRWTAFSTG ILIIPCVWLG FAVQDTSTPY SVFIIISLLC GFAGANFASS 
       160        170        180        190        200
MANISFFFPK QKQGGALGLN GGLGNMGVSV MQLVAPLVVS LSIFAVFGSQ 
       210        220        230        240        250
GVKQPDGTEL YLANASWIWV PFLAIFTIAA WFGMNDLATS KASIKEQLPV 
       260        270        280        290        300
LKRGHLWIMS LLYLATFGSF IGFSAGFAML SKTQFPDVQI LQYAFFGPFI 
       310        320        330        340        350
GALARSAGGA LSDRLGGTRV TLVNFILMAI FSGLLFLTLP TDGQGGSFMA 
       360        370        380        390        400
FFAVFLALFL TAGLGSGSTF QMISVIFRKL TMDRVKAEGG SDERAMREAA 
       410        420        430        440        450
TDTAAALGFI SAIGAIGGFF IPKAFGSSLA LTGSPVGAMK VFLIFYIACV 
       460 
VITWAVYGRH SKK
Length:463
Mass (Da):49,693
Last modified:January 1, 1990 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAF1DD67CAD40FE8A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X15996 Genomic DNA Translation: CAA34126.1
X69189 Genomic DNA Translation: CAA48933.1
U00096 Genomic DNA Translation: AAC74307.1
AP009048 Genomic DNA Translation: BAA36091.1
X13360 Genomic DNA Translation: CAA31740.1

Protein sequence database of the Protein Information Resource

More...PIRi		S05239 GRECNK

NCBI Reference Sequences

More...RefSeqi		NP_415741.1, NC_000913.3
WP_000019827.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC74307; AAC74307; b1223
BAA36091; BAA36091; BAA36091

Database of genes from NCBI RefSeq genomes

More...GeneIDi		945783

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW1214
eco:b1223

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1058

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15996 Genomic DNA Translation: CAA34126.1
X69189 Genomic DNA Translation: CAA48933.1
U00096 Genomic DNA Translation: AAC74307.1
AP009048 Genomic DNA Translation: BAA36091.1
X13360 Genomic DNA Translation: CAA31740.1
PIRiS05239 GRECNK
RefSeqiNP_415741.1, NC_000913.3
WP_000019827.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JR9X-ray2.60A1-463[»]
4JREX-ray2.80A/D1-463[»]
4U4TX-ray2.40A1-463[»]
4U4VX-ray2.35A1-463[»]
4U4WX-ray2.40A/B1-463[»]
SMRiP10903
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4261633, 21 interactors
STRINGi511145.b1223

Protein family/group databases

TCDBi2.A.1.8.1 the major facilitator superfamily (mfs)

Proteomic databases

jPOSTiP10903
PaxDbiP10903
PRIDEiP10903

Protocols and materials databases

ABCD curated depository of sequenced antibodies

More...ABCDi		P10903
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74307; AAC74307; b1223
BAA36091; BAA36091; BAA36091
GeneIDi945783
KEGGiecj:JW1214
eco:b1223
PATRICifig|1411691.4.peg.1058

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0636
EcoGeneiEG10642 narK

Phylogenomic databases

eggNOGiENOG4105DQQ Bacteria
COG2223 LUCA
HOGENOMiHOG000241722
InParanoidiP10903
KOiK02575
PhylomeDBiP10903

Enzyme and pathway databases

BioCyciEcoCyc:NARK-MONOMER
ECOL316407:JW1214-MONOMER
MetaCyc:NARK-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P10903

Family and domain databases

InterProiView protein in InterPro
IPR011701 MFS
IPR036259 MFS_trans_sf
IPR004737 NO3_transporter
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00886 2A0108, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNARK_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P10903
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: July 3, 2019
This is version 145 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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