UniProtKB - P10903 (NARK_ECOLI)
Protein
Nitrate/nitrite transporter NarK
Gene
narK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. Functions as a nitrate/nitrite exchanger, and protons are probably not co-transported with the substrate.5 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 89 | SubstrateCurated | 1 | |
Binding sitei | 305 | SubstrateCurated | 1 |
GO - Molecular functioni
- inorganic anion exchanger activity Source: EcoCyc
- nitrate transmembrane transporter activity Source: GO_Central
- nitrite efflux transmembrane transporter activity Source: GO_Central
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
- nitrate transport Source: GO_Central
- nitrite transport Source: GO_Central
Keywordsi
Biological process | Nitrate assimilation, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:NARK-MONOMER MetaCyc:NARK-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.8.1, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrate/nitrite transporter NarKAlternative name(s): Nitrite extrusion protein 1 Nitrite facilitator 1 |
Gene namesi | Name:narK Ordered Locus Names:b1223, JW1214 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 37 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 38 – 58 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 59 – 78 | PeriplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 100 – 103 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 104 – 124 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 125 – 131 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 132 – 152 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 153 – 177 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 178 – 198 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 199 – 211 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 212 – 232 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 233 – 256 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 257 – 277 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 278 – 283 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 284 – 304 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 305 – 319 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 320 – 340 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 341 – 344 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 345 – 365 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 366 – 404 | CytoplasmicSequence analysisAdd BLAST | 39 | |
Transmembranei | 405 – 425 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 426 – 435 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 436 – 456 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 457 – 463 | CytoplasmicSequence analysis | 7 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096728 | 1 – 463 | Nitrate/nitrite transporter NarKAdd BLAST | 463 |
Proteomic databases
jPOSTi | P10903 |
PaxDbi | P10903 |
PRIDEi | P10903 |
Expressioni
Inductioni
Highly expressed during anaerobic growth in the presence of nitrate.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261633, 21 interactors |
STRINGi | 511145.b1223 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10903 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2223, Bacteria |
HOGENOMi | CLU_033198_1_0_6 |
InParanoidi | P10903 |
PhylomeDBi | P10903 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR036259, MFS_trans_sf IPR004737, NO3_transporter |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00886, 2A0108, 1 hit |
i Sequence
Sequence statusi: Complete.
P10903-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHSSAPERA TGAVITDWRP EDPAFWQQRG QRIASRNLWI SVPCLLLAFC
60 70 80 90 100
VWMLFSAVAV NLPKVGFNFT TDQLFMLTAL PSVSGALLRV PYSFMVPIFG
110 120 130 140 150
GRRWTAFSTG ILIIPCVWLG FAVQDTSTPY SVFIIISLLC GFAGANFASS
160 170 180 190 200
MANISFFFPK QKQGGALGLN GGLGNMGVSV MQLVAPLVVS LSIFAVFGSQ
210 220 230 240 250
GVKQPDGTEL YLANASWIWV PFLAIFTIAA WFGMNDLATS KASIKEQLPV
260 270 280 290 300
LKRGHLWIMS LLYLATFGSF IGFSAGFAML SKTQFPDVQI LQYAFFGPFI
310 320 330 340 350
GALARSAGGA LSDRLGGTRV TLVNFILMAI FSGLLFLTLP TDGQGGSFMA
360 370 380 390 400
FFAVFLALFL TAGLGSGSTF QMISVIFRKL TMDRVKAEGG SDERAMREAA
410 420 430 440 450
TDTAAALGFI SAIGAIGGFF IPKAFGSSLA LTGSPVGAMK VFLIFYIACV
460
VITWAVYGRH SKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15996 Genomic DNA Translation: CAA34126.1 X69189 Genomic DNA Translation: CAA48933.1 U00096 Genomic DNA Translation: AAC74307.1 AP009048 Genomic DNA Translation: BAA36091.1 X13360 Genomic DNA Translation: CAA31740.1 |
PIRi | S05239, GRECNK |
RefSeqi | NP_415741.1, NC_000913.3 WP_000019827.1, NZ_STEB01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC74307; AAC74307; b1223 BAA36091; BAA36091; BAA36091 |
GeneIDi | 58460417 945783 |
KEGGi | ecj:JW1214 eco:b1223 |
PATRICi | fig|1411691.4.peg.1058 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15996 Genomic DNA Translation: CAA34126.1 X69189 Genomic DNA Translation: CAA48933.1 U00096 Genomic DNA Translation: AAC74307.1 AP009048 Genomic DNA Translation: BAA36091.1 X13360 Genomic DNA Translation: CAA31740.1 |
PIRi | S05239, GRECNK |
RefSeqi | NP_415741.1, NC_000913.3 WP_000019827.1, NZ_STEB01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4JR9 | X-ray | 2.60 | A | 1-463 | [»] | |
4JRE | X-ray | 2.80 | A/D | 1-463 | [»] | |
4U4T | X-ray | 2.40 | A | 1-463 | [»] | |
4U4V | X-ray | 2.35 | A | 1-463 | [»] | |
4U4W | X-ray | 2.40 | A/B | 1-463 | [»] | |
SMRi | P10903 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261633, 21 interactors |
STRINGi | 511145.b1223 |
Protein family/group databases
TCDBi | 2.A.1.8.1, the major facilitator superfamily (mfs) |
Proteomic databases
jPOSTi | P10903 |
PaxDbi | P10903 |
PRIDEi | P10903 |
Protocols and materials databases
ABCDi | P10903, 1 sequenced antibody |
Genome annotation databases
EnsemblBacteriai | AAC74307; AAC74307; b1223 BAA36091; BAA36091; BAA36091 |
GeneIDi | 58460417 945783 |
KEGGi | ecj:JW1214 eco:b1223 |
PATRICi | fig|1411691.4.peg.1058 |
Organism-specific databases
EchoBASEi | EB0636 |
Phylogenomic databases
eggNOGi | COG2223, Bacteria |
HOGENOMi | CLU_033198_1_0_6 |
InParanoidi | P10903 |
PhylomeDBi | P10903 |
Enzyme and pathway databases
BioCyci | EcoCyc:NARK-MONOMER MetaCyc:NARK-MONOMER |
Miscellaneous databases
PROi | PR:P10903 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR036259, MFS_trans_sf IPR004737, NO3_transporter |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00886, 2A0108, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NARK_ECOLI | |
Accessioni | P10903Primary (citable) accession number: P10903 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families