UniProtKB - P10869 (AK_YEAST)
Protein
Aspartokinase
Gene
HOM3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 48100 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.7.2.4
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Aspartokinase (HOM3)
- Aspartate-semialdehyde dehydrogenase (HOM2)
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Pathwayi: L-methionine biosynthesis via de novo pathway
This protein is involved in step 1 of the subpathway that synthesizes L-homoserine from L-aspartate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Aspartokinase (HOM3)
- Aspartate-semialdehyde dehydrogenase (HOM2)
- Homoserine dehydrogenase (HOM6)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
Pathwayi: L-threonine biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes L-threonine from L-aspartate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Aspartokinase (HOM3)
- Aspartate-semialdehyde dehydrogenase (HOM2)
- Homoserine dehydrogenase (HOM6)
- Homoserine kinase (THR1)
- Threonine synthase (THR4)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- aspartate kinase activity Source: SGD
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- homoserine biosynthetic process Source: SGD
- lysine biosynthetic process via diaminopimelate Source: GO_Central
- methionine biosynthetic process Source: SGD
- threonine biosynthetic process Source: SGD
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Amino-acid biosynthesis, Threonine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.2.4, 984 |
SABIO-RKi | P10869 |
UniPathwayi | UPA00034;UER00015 UPA00050;UER00461 UPA00051;UER00462 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HOM3 Ordered Locus Names:YER052C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000854, HOM3 |
VEuPathDBi | FungiDB:YER052C |
Subcellular locationi
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066690 | 1 – 527 | AspartokinaseAdd BLAST | 527 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 333 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P10869 |
PaxDbi | P10869 |
PRIDEi | P10869 |
PTM databases
iPTMneti | P10869 |
Interactioni
Binary interactionsi
P10869
With | #Exp. | IntAct |
---|---|---|
FPR1 [P20081] | 3 | EBI-2430,EBI-6961 |
Protein-protein interaction databases
BioGRIDi | 36791, 295 interactors |
DIPi | DIP-1319N |
IntActi | P10869, 11 interactors |
MINTi | P10869 |
STRINGi | 4932.YER052C |
Miscellaneous databases
RNActi | P10869, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 367 – 440 | ACT 1PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 442 – 527 | ACT 2PROSITE-ProRule annotationAdd BLAST | 86 |
Sequence similaritiesi
Belongs to the aspartokinase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0456, Eukaryota |
GeneTreei | ENSGT00940000176517 |
HOGENOMi | CLU_009116_6_4_1 |
InParanoidi | P10869 |
OMAi | DMIVQTI |
Family and domain databases
CDDi | cd04247, AAK_AK-Hom3, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR002912, ACT_dom IPR041747, AK-Hom3 IPR001048, Asp/Glu/Uridylate_kinase IPR001341, Asp_kinase IPR018042, Aspartate_kinase_CS IPR027795, CASTOR_ACT_dom |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF13840, ACT_7, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00657, asp_kinases, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00324, ASPARTOKINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P10869-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPMDFQPTSS HSNWVVQKFG GTSVGKFPVQ IVDDIVKHYS KPDGPNNNVA
60 70 80 90 100
VVCSARSSYT KAEGTTSRLL KCCDLASQES EFQDIIEVIR QDHIDNADRF
110 120 130 140 150
ILNPALQAKL VDDTNKELEL VKKYLNASKV LGEVSSRTVD LVMSCGEKLS
160 170 180 190 200
CLFMTALCND RGCKAKYVDL SHIVPSDFSA SALDNSFYTF LVQALKEKLA
210 220 230 240 250
PFVSAKERIV PVFTGFFGLV PTGLLNGVGR GYTDLCAALI AVAVNADELQ
260 270 280 290 300
VWKEVDGIFT ADPRKVPEAR LLDSVTPEEA SELTYYGSEV IHPFTMEQVI
310 320 330 340 350
RAKIPIRIKN VQNPLGNGTI IYPDNVAKKG ESTPPHPPEN LSSSFYEKRK
360 370 380 390 400
RGATAITTKN DIFVINIHSN KKTLSHGFLA QIFTILDKYK LVVDLISTSE
410 420 430 440 450
VHVSMALPIP DADSLKSLRQ AEEKLRILGS VDITKKLSIV SLVGKHMKQY
460 470 480 490 500
IGIAGTMFTT LAEEGINIEM ISQGANEINI SCVINESDSI KALQCIHAKL
510 520
LSERTNTSNQ FEHAIDERLE QLKRLGI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03526 Genomic DNA Translation: AAA34681.1 U18796 Genomic DNA Translation: AAB64587.1 BK006939 Genomic DNA Translation: DAA07708.1 |
PIRi | A35888, KIBYD |
RefSeqi | NP_010972.1, NM_001178943.1 |
Genome annotation databases
EnsemblFungii | YER052C_mRNA; YER052C; YER052C |
GeneIDi | 856778 |
KEGGi | sce:YER052C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03526 Genomic DNA Translation: AAA34681.1 U18796 Genomic DNA Translation: AAB64587.1 BK006939 Genomic DNA Translation: DAA07708.1 |
PIRi | A35888, KIBYD |
RefSeqi | NP_010972.1, NM_001178943.1 |
3D structure databases
SMRi | P10869 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36791, 295 interactors |
DIPi | DIP-1319N |
IntActi | P10869, 11 interactors |
MINTi | P10869 |
STRINGi | 4932.YER052C |
PTM databases
iPTMneti | P10869 |
Proteomic databases
MaxQBi | P10869 |
PaxDbi | P10869 |
PRIDEi | P10869 |
Genome annotation databases
EnsemblFungii | YER052C_mRNA; YER052C; YER052C |
GeneIDi | 856778 |
KEGGi | sce:YER052C |
Organism-specific databases
SGDi | S000000854, HOM3 |
VEuPathDBi | FungiDB:YER052C |
Phylogenomic databases
eggNOGi | KOG0456, Eukaryota |
GeneTreei | ENSGT00940000176517 |
HOGENOMi | CLU_009116_6_4_1 |
InParanoidi | P10869 |
OMAi | DMIVQTI |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00015 UPA00050;UER00461 UPA00051;UER00462 |
BRENDAi | 2.7.2.4, 984 |
SABIO-RKi | P10869 |
Miscellaneous databases
PROi | PR:P10869 |
RNActi | P10869, protein |
Family and domain databases
CDDi | cd04247, AAK_AK-Hom3, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR002912, ACT_dom IPR041747, AK-Hom3 IPR001048, Asp/Glu/Uridylate_kinase IPR001341, Asp_kinase IPR018042, Aspartate_kinase_CS IPR027795, CASTOR_ACT_dom |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF13840, ACT_7, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00657, asp_kinases, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00324, ASPARTOKINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AK_YEAST | |
Accessioni | P10869Primary (citable) accession number: P10869 Secondary accession number(s): D3DLV4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families