UniProtKB - P10861 (UCP1_BOVIN)
Mitochondrial brown fat uncoupling protein 1
UCP1
Functioni
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
By similarityActivity regulationi
GO - Molecular functioni
- cardiolipin binding Source: UniProtKB
- long-chain fatty acid binding Source: UniProtKB
- oxidative phosphorylation uncoupler activity Source: UniProtKB
- purine ribonucleotide binding Source: UniProtKB
GO - Biological processi
- adaptive thermogenesis Source: UniProtKB
- brown fat cell differentiation Source: Ensembl
- cellular response to cold Source: Ensembl
- cellular response to fatty acid Source: UniProtKB
- cellular response to hormone stimulus Source: UniProtKB
- cellular response to reactive oxygen species Source: UniProtKB
- diet induced thermogenesis Source: Ensembl
- mitochondrial transmembrane transport Source: UniProtKB
- mitochondrial transport Source: InterPro
- positive regulation of cold-induced thermogenesis Source: Ensembl
- proton transmembrane transport Source: UniProtKB
- regulation of reactive oxygen species biosynthetic process Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: Ensembl
- response to cold Source: GO_Central
- response to nutrient levels Source: UniProtKB
- response to temperature stimulus Source: UniProtKB
Keywordsi
Molecular function | Ion channel |
Biological process | Ion transport, Transport |
Protein family/group databases
TCDBi | 2.A.29.3.1, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial brown fat uncoupling protein 1CuratedShort name: UCP 1Curated Alternative name(s): Solute carrier family 25 member 7By similarity ThermogeninBy similarity |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000004647 |
VGNCi | VGNC:36640, UCP1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 10 | Mitochondrial intermembraneCurated | 9 | |
Transmembranei | 11 – 32 | Helical; Name=1Sequence analysisAdd BLAST | 22 | |
Topological domaini | 33 – 77 | Mitochondrial matrixCuratedAdd BLAST | 45 | |
Transmembranei | 78 – 100 | Helical; Name=2Sequence analysisAdd BLAST | 23 | |
Topological domaini | 101 – 118 | Mitochondrial intermembraneCuratedAdd BLAST | 18 | |
Transmembranei | 119 – 135 | Helical; Name=3Sequence analysisAdd BLAST | 17 | |
Topological domaini | 136 – 180 | Mitochondrial matrixCuratedAdd BLAST | 45 | |
Transmembranei | 181 – 197 | Helical; Name=4Sequence analysisAdd BLAST | 17 | |
Topological domaini | 198 – 214 | Mitochondrial intermembraneCuratedAdd BLAST | 17 | |
Transmembranei | 215 – 234 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Topological domaini | 235 – 268 | Mitochondrial matrixCuratedAdd BLAST | 34 | |
Transmembranei | 269 – 291 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 292 – 309 | Mitochondrial intermembraneCuratedAdd BLAST | 18 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090654 | 1 – 309 | Mitochondrial brown fat uncoupling protein 1Add BLAST | 309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 256 | Cysteine sulfenic acid (-SOH)By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
OxidationProteomic databases
PaxDbi | P10861 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000004647, Expressed in skeletal muscle tissue and 17 other tissues |
Interactioni
Subunit structurei
Most probably functions as a monomer. Binds one purine nucleotide per monomer. However, has also been suggested to function as a homodimer or a homotetramer. Tightly associates with cardiolipin in the mitochondrion inner membrane; may stabilize and regulate its activity.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000006097 |
Structurei
3D structure databases
AlphaFoldDBi | P10861 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 11 – 106 | Solcar 1PROSITE-ProRule annotationAdd BLAST | 96 | |
Repeati | 113 – 203 | Solcar 2PROSITE-ProRule annotationAdd BLAST | 91 | |
Repeati | 212 – 297 | Solcar 3PROSITE-ProRule annotationAdd BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0753, Eukaryota |
GeneTreei | ENSGT00940000160382 |
InParanoidi | P10861 |
OMAi | YTSVPNC |
OrthoDBi | 984118at2759 |
TreeFami | TF323211 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR040011, UCP1 |
PANTHERi | PTHR45618:SF19, PTHR45618:SF19, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVGHTESDVP PTMAVKIFSA GVAACVADII TFPLDTAKVR LQVGSAIQGE
60 70 80 90 100
CLISSAIRYK GVLGTIITLA KTEGPVKLYS GLPAGLQRQI SFASLRIGLY
110 120 130 140 150
DTVQEFFTTG KEASLGSKIS AGLMTGGVAV FIGQPTEVVK VRLQAQSHLH
160 170 180 190 200
GPKPRYTGTY NAYRIIATTE GLTGLWKGTT PNLTRNVIIN CTELVTYDLM
210 220 230 240 250
KEALVKNKLL ADDVPCHFVS AVVAGFCTTV LSSPVDVVKT RFVNSSPGQY
260 270 280 290 300
TSVPNCAMMM LTREGPSAFF KGFVPSFLRL GSWNIIMFVC FEQLKQELMK
SRHTMDCAT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 – 46 | Missing in CAA32227 (PubMed:2928121).Curated | 4 | |
Sequence conflicti | 92 | F → L in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 180 | T → S in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 185 | R → T in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 250 | Y → N in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 285 | Missing in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 293 | Q → R in CAA32227 (PubMed:2928121).Curated | 1 | |
Sequence conflicti | 301 | S → C in CAA32227 (PubMed:2928121).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02044420 Genomic DNA No translation available. DAAA02044421 Genomic DNA No translation available. X14064 mRNA Translation: CAA32227.1 |
PIRi | S03603 |
RefSeqi | NP_001160000.1, NM_001166528.1 |
Genome annotation databases
Ensembli | ENSBTAT00000006097; ENSBTAP00000006097; ENSBTAG00000004647 |
GeneIDi | 281561 |
KEGGi | bta:281561 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02044420 Genomic DNA No translation available. DAAA02044421 Genomic DNA No translation available. X14064 mRNA Translation: CAA32227.1 |
PIRi | S03603 |
RefSeqi | NP_001160000.1, NM_001166528.1 |
3D structure databases
AlphaFoldDBi | P10861 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000006097 |
Protein family/group databases
TCDBi | 2.A.29.3.1, the mitochondrial carrier (mc) family |
Proteomic databases
PaxDbi | P10861 |
Genome annotation databases
Ensembli | ENSBTAT00000006097; ENSBTAP00000006097; ENSBTAG00000004647 |
GeneIDi | 281561 |
KEGGi | bta:281561 |
Organism-specific databases
CTDi | 7350 |
VEuPathDBi | HostDB:ENSBTAG00000004647 |
VGNCi | VGNC:36640, UCP1 |
Phylogenomic databases
eggNOGi | KOG0753, Eukaryota |
GeneTreei | ENSGT00940000160382 |
InParanoidi | P10861 |
OMAi | YTSVPNC |
OrthoDBi | 984118at2759 |
TreeFami | TF323211 |
Gene expression databases
Bgeei | ENSBTAG00000004647, Expressed in skeletal muscle tissue and 17 other tissues |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR040011, UCP1 |
PANTHERi | PTHR45618:SF19, PTHR45618:SF19, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | UCP1_BOVIN | |
Accessioni | P10861Primary (citable) accession number: P10861 Secondary accession number(s): F1MTU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 2, 2016 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families