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Protein

Histone H2B type 1-F/J/L

Gene

Hist1h2bf

more
Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

  • DNA binding Source: GO_Central
  • identical protein binding Source: MGI
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B type 1-F/J/L
Alternative name(s):
H2B 291A
Gene namesi
Name:Hist1h2bf
Synonyms:H2b-f
AND
Name:Hist1h2bj
Synonyms:H2b-j
AND
Name:Hist1h2bl
Synonyms:H2b-l
AND
Name:Hist1h2bn
Synonyms:H2b-n
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2448383 Hist1h2bf
MGI:2448388 Hist1h2bj
MGI:2448403 Hist1h2bl
MGI:2448407 Hist1h2bn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000718392 – 126Histone H2B type 1-F/J/LAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylprolineBy similarity1
Modified residuei6N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei6N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei6N6-acetyllysine; alternateBy similarity1
Modified residuei6N6-butyryllysine; alternateBy similarity1
Modified residuei6N6-crotonyllysine; alternate1 Publication1
Cross-linki6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei12N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei12N6-acetyllysine; alternateBy similarity1
Modified residuei12N6-crotonyllysine; alternate1 Publication1
Modified residuei13N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei13N6-acetyllysine; alternateBy similarity1
Modified residuei13N6-crotonyllysine; alternate1 Publication1
Modified residuei15Phosphoserine; by STK4/MST12 Publications1
Modified residuei16N6-acetyllysine; alternateBy similarity1
Modified residuei16N6-crotonyllysine; alternate1 Publication1
Modified residuei17N6-acetyllysine; alternateBy similarity1
Modified residuei17N6-crotonyllysine; alternate1 Publication1
Modified residuei21N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei21N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei21N6-acetyllysine; alternateBy similarity1
Modified residuei21N6-butyryllysine; alternateBy similarity1
Modified residuei21N6-crotonyllysine; alternate1 Publication1
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei24N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei24N6-acetyllysine; alternateBy similarity1
Modified residuei24N6-crotonyllysine; alternate1 Publication1
Modified residuei25N6-(2-hydroxyisobutyryl)lysine1 Publication1
Modified residuei35N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei35N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei35N6-crotonyllysine; alternate1 Publication1
Modified residuei35N6-succinyllysine; alternateBy similarity1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei37Phosphoserine; by AMPK1 Publication1
Modified residuei44N6-(2-hydroxyisobutyryl)lysine1 Publication1
Modified residuei47N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei47N6-methyllysine; alternateBy similarity1
Modified residuei58N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei58N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei80Dimethylated arginineBy similarity1
Modified residuei86N6,N6,N6-trimethyllysine; alternateBy similarity1
Modified residuei86N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei86N6-acetyllysine; alternateBy similarity1
Modified residuei87Omega-N-methylarginineBy similarity1
Modified residuei93Omega-N-methylarginineBy similarity1
Modified residuei109N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei109N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei109N6-methyllysine; alternateBy similarity1
Glycosylationi113O-linked (GlcNAc) serineBy similarity1
Modified residuei116PhosphothreonineBy similarity1
Modified residuei117N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei117N6-(beta-hydroxybutyryl)lysine; alternate1 Publication1
Modified residuei117N6-methylated lysine; alternateBy similarity1
Modified residuei117N6-succinyllysine; alternateBy similarity1
Modified residuei121N6-(2-hydroxyisobutyryl)lysine; alternate1 Publication1
Modified residuei121N6-succinyllysine; alternate1 Publication1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons (By similarity).By similarity
Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription.3 Publications
GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.1 Publication
Hydroxybutyrylation of histones is induced by starvation.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Hydroxylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10853
PaxDbiP10853
PeptideAtlasiP10853
PRIDEiP10853
TopDownProteomicsiP10853

PTM databases

iPTMnetiP10853
PhosphoSitePlusiP10853

Expressioni

Gene expression databases

BgeeiENSMUSG00000069268 Expressed in 21 organ(s), highest expression level in thymus
CleanExiMM_HIST1H2BF
MM_HIST1H2BJ
MM_HIST1H2BL
MM_HIST1H2BN
ExpressionAtlasiP10853 baseline and differential
GenevisibleiP10853 MM

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi235104, 1 interactor
IntActiP10853, 2 interactors
MINTiP10853
STRINGi10090.ENSMUSP00000106082

Structurei

3D structure databases

ProteinModelPortaliP10853
SMRiP10853
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744 Eukaryota
ENOG4111NV5 LUCA
GeneTreeiENSGT00760000118976
HOGENOMiHOG000231213
HOVERGENiHBG007774
InParanoidiP10853
KOiK11252
OMAiSSMAMNI
OrthoDBiEOG091G0XGD
PhylomeDBiP10853
TreeFamiTF300212

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000558 Histone_H2B
PANTHERiPTHR23428 PTHR23428, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00621 HISTONEH2B
SMARTiView protein in SMART
SM00427 H2B, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00357 HISTONE_H2B, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P10853-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEPAKSAPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH
60 70 80 90 100
PDTGISSKAM GIMNSFVNDI FERIASEASR LAHYNKRSTI TSREIQTAVR
110 120
LLLPGELAKH AVSEGTKAVT KYTSSK
Length:126
Mass (Da):13,936
Last modified:January 23, 2007 - v2
Checksum:iFAE15CE99FC8B03D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8CBB6Q8CBB6_MOUSE
Histone H2B
Hist1h2bq Hist1h2br, mCG_50238
134Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05862 Genomic DNA Translation: CAA29290.1
U62669 Genomic DNA Translation: AAB04762.1
AY158927 Genomic DNA Translation: AAO06237.1
AY158929 Genomic DNA Translation: AAO06239.1
AY158932 Genomic DNA Translation: AAO06242.1
AY158935 Genomic DNA Translation: AAO06245.1
AL589651 Genomic DNA Translation: CAI24103.1
AL589651 Genomic DNA Translation: CAI24111.1
AL589879 Genomic DNA Translation: CAI25462.1
AL589879 Genomic DNA Translation: CAI25467.1
AL590614 Genomic DNA Translation: CAI26130.1
CCDSiCCDS26287.1
CCDS26294.1
CCDS26308.1
CCDS26350.1
PIRiS04151
RefSeqiNP_001300807.1, NM_001313878.1
NP_001300809.1, NM_001313880.1
NP_835502.1, NM_178195.2
NP_835505.1, NM_178198.2
NP_835506.1, NM_178199.2
NP_835508.1, NM_178201.2
UniGeneiMm.371766
Mm.471247

Genome annotation databases

EnsembliENSMUST00000078156; ENSMUSP00000077290; ENSMUSG00000069307
ENSMUST00000091709; ENSMUSP00000089301; ENSMUSG00000095217
ENSMUST00000091756; ENSMUSP00000089350; ENSMUSG00000094338
ENSMUST00000105106; ENSMUSP00000100738; ENSMUSG00000069268
ENSMUST00000110452; ENSMUSP00000106082; ENSMUSG00000069300
ENSMUST00000110467; ENSMUSP00000106093; ENSMUSG00000069303
ENSMUST00000110469; ENSMUSP00000106095; ENSMUSG00000069307
ENSMUST00000110476; ENSMUSP00000106102; ENSMUSG00000069303
GeneIDi319180
319183
319185
319187
665596
665622
KEGGimmu:319180
mmu:319183
mmu:319185
mmu:319187
mmu:665596
mmu:665622
UCSCiuc007pra.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05862 Genomic DNA Translation: CAA29290.1
U62669 Genomic DNA Translation: AAB04762.1
AY158927 Genomic DNA Translation: AAO06237.1
AY158929 Genomic DNA Translation: AAO06239.1
AY158932 Genomic DNA Translation: AAO06242.1
AY158935 Genomic DNA Translation: AAO06245.1
AL589651 Genomic DNA Translation: CAI24103.1
AL589651 Genomic DNA Translation: CAI24111.1
AL589879 Genomic DNA Translation: CAI25462.1
AL589879 Genomic DNA Translation: CAI25467.1
AL590614 Genomic DNA Translation: CAI26130.1
CCDSiCCDS26287.1
CCDS26294.1
CCDS26308.1
CCDS26350.1
PIRiS04151
RefSeqiNP_001300807.1, NM_001313878.1
NP_001300809.1, NM_001313880.1
NP_835502.1, NM_178195.2
NP_835505.1, NM_178198.2
NP_835506.1, NM_178199.2
NP_835508.1, NM_178201.2
UniGeneiMm.371766
Mm.471247

3D structure databases

ProteinModelPortaliP10853
SMRiP10853
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235104, 1 interactor
IntActiP10853, 2 interactors
MINTiP10853
STRINGi10090.ENSMUSP00000106082

PTM databases

iPTMnetiP10853
PhosphoSitePlusiP10853

Proteomic databases

MaxQBiP10853
PaxDbiP10853
PeptideAtlasiP10853
PRIDEiP10853
TopDownProteomicsiP10853

Protocols and materials databases

DNASUi319180
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078156; ENSMUSP00000077290; ENSMUSG00000069307
ENSMUST00000091709; ENSMUSP00000089301; ENSMUSG00000095217
ENSMUST00000091756; ENSMUSP00000089350; ENSMUSG00000094338
ENSMUST00000105106; ENSMUSP00000100738; ENSMUSG00000069268
ENSMUST00000110452; ENSMUSP00000106082; ENSMUSG00000069300
ENSMUST00000110467; ENSMUSP00000106093; ENSMUSG00000069303
ENSMUST00000110469; ENSMUSP00000106095; ENSMUSG00000069307
ENSMUST00000110476; ENSMUSP00000106102; ENSMUSG00000069303
GeneIDi319180
319183
319185
319187
665596
665622
KEGGimmu:319180
mmu:319183
mmu:319185
mmu:319187
mmu:665596
mmu:665622
UCSCiuc007pra.2 mouse

Organism-specific databases

CTDi665596
665622
8340
8341
8343
8970
MGIiMGI:2448383 Hist1h2bf
MGI:2448388 Hist1h2bj
MGI:2448403 Hist1h2bl
MGI:2448407 Hist1h2bn

Phylogenomic databases

eggNOGiKOG1744 Eukaryota
ENOG4111NV5 LUCA
GeneTreeiENSGT00760000118976
HOGENOMiHOG000231213
HOVERGENiHBG007774
InParanoidiP10853
KOiK11252
OMAiSSMAMNI
OrthoDBiEOG091G0XGD
PhylomeDBiP10853
TreeFamiTF300212

Miscellaneous databases

PROiPR:P10853
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000069268 Expressed in 21 organ(s), highest expression level in thymus
CleanExiMM_HIST1H2BF
MM_HIST1H2BJ
MM_HIST1H2BL
MM_HIST1H2BN
ExpressionAtlasiP10853 baseline and differential
GenevisibleiP10853 MM

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000558 Histone_H2B
PANTHERiPTHR23428 PTHR23428, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00621 HISTONEH2B
SMARTiView protein in SMART
SM00427 H2B, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00357 HISTONE_H2B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiH2B1F_MOUSE
AccessioniPrimary (citable) accession number: P10853
Secondary accession number(s): Q5SZZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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