UniProtKB - P10824 (GNAI1_RAT)
Guanine nucleotide-binding protein G(i) subunit alpha-1
Gnai1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumCombined sources2 Publications | 1 | |
Metal bindingi | 181 | MagnesiumCombined sources3 Publications | 1 | |
Binding sitei | 326 | GTP; via amide nitrogenCombined sources | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 48 | GTPCombined sources | 6 | |
Nucleotide bindingi | 150 – 151 | GTPCombined sources | 2 | |
Nucleotide bindingi | 175 – 178 | GTPCombined sources | 4 | |
Nucleotide bindingi | 200 – 204 | GTPCombined sources | 5 | |
Nucleotide bindingi | 269 – 272 | GTPCombined sources | 4 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: UniProtKB
- G protein-coupled serotonin receptor binding Source: RGD
- GTPase activating protein binding Source: RGD
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
GO - Biological processi
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB
- cellular response to forskolin Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of synaptic transmission Source: RGD
- positive regulation of protein localization to cell cortex Source: UniProtKB
- regulation of cAMP-mediated signaling Source: UniProtKB
- regulation of mitotic spindle organization Source: UniProtKB
Keywordsi
Molecular function | Transducer |
Biological process | Cell cycle, Cell division, Mitosis, Transport |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-112043, PLC beta mediated events R-RNO-170670, Adenylate cyclase inhibitory pathway R-RNO-202040, G-protein activation R-RNO-392170, ADP signalling through P2Y purinoceptor 12 R-RNO-400042, Adrenaline,noradrenaline inhibits insulin secretion R-RNO-418594, G alpha (i) signalling events R-RNO-9009391, Extra-nuclear estrogen signaling |
SABIO-RKi | P10824 |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-1Alternative name(s): Adenylate cyclase-inhibiting G alpha protein |
Gene namesi | Name:Gnai1 Synonyms:Gnai-1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2713, Gnai1 |
Subcellular locationi
Nucleus
Plasma membrane
- Cell membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side Curated
Cytoskeleton
- centrosome 1 Publication
Other locations
- Cytoplasm 1 Publication
- cell cortex By similarity
- Membrane 1 Publication; Lipid-anchor 1 Publication
Note: Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane.1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cell cortex region Source: UniProtKB
- cytoplasm Source: UniProtKB
- membrane Source: RGD
- membrane raft Source: RGD
- midbody Source: UniProtKB
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Abolishes myristoylation and palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 3 | C → S: Abolishes palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 43 | E → A: Mildly impairs receptor binding; mildly decreases basal and receptor-stimulated GDP exchange. 1 Publication | 1 | |
Mutagenesisi | 149 | N → I: Inhibits interaction with RGS14. Does not inhibit interaction with RIC8A. 1 Publication | 1 | |
Mutagenesisi | 189 | F → Y: Increases basal GDP exchange rate; no effect on receptor-stimulated GDP exchange. 1 Publication | 1 | |
Mutagenesisi | 191 | F → Y: No effect on basal GDP exchange rate; mildly decreases receptor-stimulated GDP exchange. 1 Publication | 1 | |
Mutagenesisi | 204 | Q → L: Expected to have lost GTPase activity; inhibits the forskolin-mediated increase of cellular cAMP levels. Does not inhibit interaction with RGS14 at centrosomes. 2 Publications | 1 | |
Mutagenesisi | 329 | T → A: Increases basal GDP exchange rate and inhibits the forskolin-mediated increase of cellular cAMP levels. 1 Publication | 1 | |
Mutagenesisi | 332 | V → A: Increases basal GDP exchange rate. 1 Publication | 1 | |
Mutagenesisi | 336 | F → A or C: Increases basal GDP exchange rate; mildly decreases receptor-stimulated GDP exchange. 1 Publication | 1 | |
Mutagenesisi | 336 | F → Y: Strongly increases basal GDP exchange rate; mildly decreases receptor-stimulated GDP exchange. 1 Publication | 1 | |
Mutagenesisi | 345 | K → L: Mildly impairs receptor binding; mildly decreases basal and receptor-stimulated GDP exchange. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000203673 | 2 – 354 | Guanine nucleotide-binding protein G(i) subunit alpha-1Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Lipidationi | 3 | S-palmitoyl cysteine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
jPOSTi | P10824 |
PRIDEi | P10824 |
2D gel databases
World-2DPAGEi | 0004:P10824 |
PTM databases
iPTMneti | P10824 |
PhosphoSitePlusi | P10824 |
SwissPalmi | P10824 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000057096, Expressed in brain and 20 other tissues |
Interactioni
Subunit structurei
Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (PubMed:8521505, PubMed:24596087, PubMed:25037222).
Part of a spindle orientation complex at least composed of GNAI1, GPSM2 and NUMA1 (By similarity). Identified in complex with the beta subunit GNB1 and the gamma subunit GNG1 (PubMed:24596087). Identified in complex with the beta subunit GNB1 and the gamma subunit GNG2 (PubMed:8521505). GTP binding causes dissociation of the heterotrimer, liberating the individual subunits so that they can interact with downstream effector proteins.
Interacts (GDP-bound form) with GPSM1; this inhibits guanine nucleotide exchange and GTP binding (PubMed:11121039).
Interacts (GDP-bound form) with GPSM2 (via GoLoco domains); this inhibits guanine nucleotide exchange (By similarity).
Interacts with RGS10; this strongly enhances GTP hydrolysis.
Interacts with RGS1 and RGS16 (By similarity).
Interacts with RGS4 (PubMed:9108480).
Interacts with RGS12 (PubMed:11387333).
Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains) (PubMed:11387333, PubMed:16870394, PubMed:17635935, PubMed:21158412).
Interacts with RGS3, RGS6, RGS7, RGS8, RGS17, RGS18 and RGS20 (in vitro) (By similarity).
Interacts (GDP-bound form) with RIC8A (via C-terminus) (PubMed:21158412).
Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI1 (PubMed:21653697).
Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif); the interaction leads to activation of GNAI1 (By similarity).
Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (PubMed:19211784).
By similarity10 PublicationsGO - Molecular functioni
- G protein-coupled receptor binding Source: UniProtKB
- G protein-coupled serotonin receptor binding Source: RGD
- GTPase activating protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247715, 2 interactors |
CORUMi | P10824 |
DIPi | DIP-6073N |
IntActi | P10824, 2 interactors |
MINTi | P10824 |
Structurei
Secondary structure
3D structure databases
SASBDBi | P10824 |
SMRi | P10824 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10824 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 354 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 173 – 181 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 196 – 205 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 265 – 272 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 324 – 329 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00940000153567 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P10824 |
OMAi | RIAQTSY |
OrthoDBi | 754573at2759 |
PhylomeDBi | P10824 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEAG YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDAAR
110 120 130 140 150
ADDARQLFVL AGAAEEGFMT AELAGVIKRL WKDSGVQACF NRSREYQLND
160 170 180 190 200
SAAYYLNDLD RIAQPNYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD
210 220 230 240 250
VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF
260 270 280 290 300
DSICNNKWFT DTSIILFLNK KDLFEEKIKK SPLTICYPEY AGSNTYEEAA
310 320 330 340 350
AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD
CGLF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17527 mRNA Translation: AAA40825.1 DQ120469 mRNA Translation: AAZ23808.1 DQ120470 mRNA Translation: AAZ23809.1 |
PIRi | A35377 C27423, RGRTI1 |
RefSeqi | NP_037277.1, NM_013145.1 |
Genome annotation databases
Ensembli | ENSRNOT00000091004; ENSRNOP00000074036; ENSRNOG00000057096 |
GeneIDi | 25686 |
KEGGi | rno:25686 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17527 mRNA Translation: AAA40825.1 DQ120469 mRNA Translation: AAZ23808.1 DQ120470 mRNA Translation: AAZ23809.1 |
PIRi | A35377 C27423, RGRTI1 |
RefSeqi | NP_037277.1, NM_013145.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AGR | X-ray | 2.80 | A/D | 2-354 | [»] | |
1AS0 | X-ray | 2.00 | A | 2-354 | [»] | |
1AS2 | X-ray | 2.80 | A | 2-354 | [»] | |
1AS3 | X-ray | 2.40 | A | 2-354 | [»] | |
1BH2 | X-ray | 2.10 | A | 32-346 | [»] | |
1BOF | X-ray | 2.20 | A | 2-354 | [»] | |
1CIP | X-ray | 1.50 | A | 2-354 | [»] | |
1FQJ | X-ray | 2.02 | A/D | 220-299 | [»] | |
1FQK | X-ray | 2.30 | A/C | 220-299 | [»] | |
1GDD | X-ray | 2.20 | A | 2-354 | [»] | |
1GFI | X-ray | 2.20 | A | 2-354 | [»] | |
1GG2 | X-ray | 2.40 | A | 2-354 | [»] | |
1GIA | X-ray | 2.00 | A | 2-354 | [»] | |
1GIL | X-ray | 2.30 | A | 2-354 | [»] | |
1GIT | X-ray | 2.60 | A | 2-354 | [»] | |
1GP2 | X-ray | 2.30 | A | 2-354 | [»] | |
1SHZ | X-ray | 2.85 | A/D | 22-48 | [»] | |
A/D | 185-353 | [»] | ||||
1SVK | X-ray | 2.00 | A | 2-354 | [»] | |
1SVS | X-ray | 1.50 | A | 2-354 | [»] | |
2BCJ | X-ray | 3.06 | Q | 1-28 | [»] | |
2ZJY | X-ray | 2.80 | A | 1-354 | [»] | |
2ZJZ | X-ray | 2.60 | A/B | 1-354 | [»] | |
3AH8 | X-ray | 2.90 | A | 2-28 | [»] | |
3D7M | X-ray | 2.90 | A | 1-354 | [»] | |
3FFA | X-ray | 2.30 | A | 1-354 | [»] | |
3FFB | X-ray | 2.57 | A | 1-354 | [»] | |
3V00 | X-ray | 2.90 | A/B/C | 220-298 | [»] | |
4N0D | X-ray | 1.55 | A | 1-354 | [»] | |
4N0E | X-ray | 2.10 | A | 1-354 | [»] | |
4PAM | X-ray | 2.10 | A | 1-354 | [»] | |
4PAN | X-ray | 2.40 | A | 1-354 | [»] | |
4PAO | X-ray | 2.00 | A | 1-354 | [»] | |
4PAQ | X-ray | 2.00 | A | 1-354 | [»] | |
5KDL | X-ray | 2.67 | A/B | 1-354 | [»] | |
5KDO | X-ray | 1.90 | A | 1-354 | [»] | |
6M8H | X-ray | 2.07 | A | 1-354 | [»] | |
6TYL | X-ray | 3.30 | B/G | 1-354 | [»] | |
6UKT | electron microscopy | 3.87 | B | 32-354 | [»] | |
6VMS | electron microscopy | 3.80 | A | 1-354 | [»] | |
SASBDBi | P10824 | |||||
SMRi | P10824 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247715, 2 interactors |
CORUMi | P10824 |
DIPi | DIP-6073N |
IntActi | P10824, 2 interactors |
MINTi | P10824 |
PTM databases
iPTMneti | P10824 |
PhosphoSitePlusi | P10824 |
SwissPalmi | P10824 |
2D gel databases
World-2DPAGEi | 0004:P10824 |
Proteomic databases
jPOSTi | P10824 |
PRIDEi | P10824 |
Protocols and materials databases
ABCDi | P10824, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000091004; ENSRNOP00000074036; ENSRNOG00000057096 |
GeneIDi | 25686 |
KEGGi | rno:25686 |
Organism-specific databases
CTDi | 2770 |
RGDi | 2713, Gnai1 |
Phylogenomic databases
GeneTreei | ENSGT00940000153567 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P10824 |
OMAi | RIAQTSY |
OrthoDBi | 754573at2759 |
PhylomeDBi | P10824 |
Enzyme and pathway databases
Reactomei | R-RNO-112043, PLC beta mediated events R-RNO-170670, Adenylate cyclase inhibitory pathway R-RNO-202040, G-protein activation R-RNO-392170, ADP signalling through P2Y purinoceptor 12 R-RNO-400042, Adrenaline,noradrenaline inhibits insulin secretion R-RNO-418594, G alpha (i) signalling events R-RNO-9009391, Extra-nuclear estrogen signaling |
SABIO-RKi | P10824 |
Miscellaneous databases
EvolutionaryTracei | P10824 |
PROi | PR:P10824 |
Gene expression databases
Bgeei | ENSRNOG00000057096, Expressed in brain and 20 other tissues |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAI1_RAT | |
Accessioni | P10824Primary (citable) accession number: P10824 Secondary accession number(s): Q45QN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families