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Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:16148141). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135, PubMed:15895089). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (By similarity). Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity).By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-140534 via Death Receptors in the presence of ligand
R-MMU-166016 Toll Like Receptor 4 (TLR4) Cascade
R-MMU-166020 Transfer of LPS from LBP carrier to CD14
R-MMU-166166 MyD88-independent TLR4 cascade
R-MMU-2562578 TRIF-mediated programmed cell death
R-MMU-5686938 Regulation of TLR by endogenous ligand
R-MMU-6798695 Neutrophil degranulation
R-MMU-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-MMU-937041 IKK complex recruitment mediated by RIP1
R-MMU-937072 TRAF6-mediated induction of TAK1 complex within TLR4 complex

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:Cd14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:88318 Cd14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice are fertile and appear healthy when kept in a clean, microbe-free environment. Mice do not respond to bacterial smooth lipopolysaccharide (LPS). Contrary to wild-type, they do not develop toxic shock or secrete TNF in response to LPS. Surprisingly, they have fewer live bacteria in their lungs and bloodstream after inoculation with bacteria and are not killed by an inoculum that is lethal to wild-type; they are killed when the inoculum is further increased.1 Publication

Chemistry databases

ChEMBLiCHEMBL2384896

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
ChainiPRO_000002088716 – 336Monocyte differentiation antigen CD14Add BLAST321
PropeptideiPRO_0000020888337 – 366Removed in mature formSequence analysisAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 321 Publication
Disulfide bondi30 ↔ 471 Publication
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi147N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi180N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi183 ↔ 2131 Publication
Disulfide bondi237 ↔ 2661 Publication
Glycosylationi276N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi317N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi336GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP10810
PeptideAtlasiP10810
PRIDEiP10810

PTM databases

iPTMnetiP10810
PhosphoSitePlusiP10810
SwissPalmiP10810

Miscellaneous databases

PMAP-CutDBiP10810

Expressioni

Tissue specificityi

Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).2 Publications

Gene expression databases

BgeeiENSMUSG00000051439 Expressed in 143 organ(s), highest expression level in inguinal mammary gland
CleanExiMM_CD14
ExpressionAtlasiP10810 baseline and differential
GenevisibleiP10810 MM

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (By similarity). Interacts with LPS-bound LPB. Interacts with LPAR1 (PubMed:21821728). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively. Interacts with MYO18A (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi198573, 2 interactors
STRINGi10090.ENSMUSP00000056669

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10810
SMRiP10810
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10810

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 78LRR 1Add BLAST29
Repeati79 – 114LRR 2Add BLAST36
Repeati115 – 140LRR 3Add BLAST26
Repeati141 – 168LRR 4Add BLAST28
Repeati169 – 192LRR 5Add BLAST24
Repeati193 – 220LRR 6Add BLAST28
Repeati221 – 247LRR 7Add BLAST27
Repeati248 – 272LRR 8Add BLAST25
Repeati273 – 293LRR 9Add BLAST21
Repeati294 – 315LRR 10Add BLAST22
Repeati316 – 340LRR 11Add BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 366Required for response to bacterial lipopolysaccharide (LPS)1 PublicationAdd BLAST83

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.1 Publication

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9 Eukaryota
ENOG41118N2 LUCA
GeneTreeiENSGT00390000005689
HOGENOMiHOG000237268
HOVERGENiHBG005269
InParanoidiP10810
KOiK04391
OMAiLCPHKFP
OrthoDBiEOG091G0DA3
PhylomeDBiP10810
TreeFamiTF338550

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR016337 Monocyte_diff_Ag_CD14
PANTHERiPTHR10630 PTHR10630, 1 hit
PIRSFiPIRSF002017 CD14, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10810-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERVLGLLLL LLVHASPAPP EPCELDEESC SCNFSDPKPD WSSAFNCLGA
60 70 80 90 100
ADVELYGGGR SLEYLLKRVD TEADLGQFTD IIKSLSLKRL TVRAARIPSR
110 120 130 140 150
ILFGALRVLG ISGLQELTLE NLEVTGTAPP PLLEATGPDL NILNLRNVSW
160 170 180 190 200
ATRDAWLAEL QQWLKPGLKV LSIAQAHSLN FSCEQVRVFP ALSTLDLSDN
210 220 230 240 250
PELGERGLIS ALCPLKFPTL QVLALRNAGM ETPSGVCSAL AAARVQLQGL
260 270 280 290 300
DLSHNSLRDA AGAPSCDWPS QLNSLNLSFT GLKQVPKGLP AKLSVLDLSY
310 320 330 340 350
NRLDRNPSPD ELPQVGNLSL KGNPFLDSES HSEKFNSGVV TAGAPSSQAV
360
ALSGTLALLL GDRLFV
Length:366
Mass (Da):39,204
Last modified:July 1, 1989 - v1
Checksum:i57C4492EC7EA3AA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13987 Genomic DNA Translation: CAA32166.1
X13333 mRNA Translation: CAA31710.1
M34510 Genomic DNA Translation: AAA37387.1
BC057889 mRNA Translation: AAH57889.1
CCDSiCCDS29159.1
PIRiS03605 TDMSM4
RefSeqiNP_033971.1, NM_009841.4
UniGeneiMm.3460

Genome annotation databases

EnsembliENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439
GeneIDi12475
KEGGimmu:12475
UCSCiuc008eof.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13987 Genomic DNA Translation: CAA32166.1
X13333 mRNA Translation: CAA31710.1
M34510 Genomic DNA Translation: AAA37387.1
BC057889 mRNA Translation: AAH57889.1
CCDSiCCDS29159.1
PIRiS03605 TDMSM4
RefSeqiNP_033971.1, NM_009841.4
UniGeneiMm.3460

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WWLX-ray2.50A/B20-329[»]
ProteinModelPortaliP10810
SMRiP10810
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198573, 2 interactors
STRINGi10090.ENSMUSP00000056669

Chemistry databases

ChEMBLiCHEMBL2384896

PTM databases

iPTMnetiP10810
PhosphoSitePlusiP10810
SwissPalmiP10810

Proteomic databases

PaxDbiP10810
PeptideAtlasiP10810
PRIDEiP10810

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061829; ENSMUSP00000056669; ENSMUSG00000051439
GeneIDi12475
KEGGimmu:12475
UCSCiuc008eof.2 mouse

Organism-specific databases

CTDi929
MGIiMGI:88318 Cd14

Phylogenomic databases

eggNOGiENOG410IXG9 Eukaryota
ENOG41118N2 LUCA
GeneTreeiENSGT00390000005689
HOGENOMiHOG000237268
HOVERGENiHBG005269
InParanoidiP10810
KOiK04391
OMAiLCPHKFP
OrthoDBiEOG091G0DA3
PhylomeDBiP10810
TreeFamiTF338550

Enzyme and pathway databases

ReactomeiR-MMU-140534 via Death Receptors in the presence of ligand
R-MMU-166016 Toll Like Receptor 4 (TLR4) Cascade
R-MMU-166020 Transfer of LPS from LBP carrier to CD14
R-MMU-166166 MyD88-independent TLR4 cascade
R-MMU-2562578 TRIF-mediated programmed cell death
R-MMU-5686938 Regulation of TLR by endogenous ligand
R-MMU-6798695 Neutrophil degranulation
R-MMU-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-MMU-937041 IKK complex recruitment mediated by RIP1
R-MMU-937072 TRAF6-mediated induction of TAK1 complex within TLR4 complex

Miscellaneous databases

EvolutionaryTraceiP10810
PMAP-CutDBiP10810
PROiPR:P10810
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051439 Expressed in 143 organ(s), highest expression level in inguinal mammary gland
CleanExiMM_CD14
ExpressionAtlasiP10810 baseline and differential
GenevisibleiP10810 MM

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR016337 Monocyte_diff_Ag_CD14
PANTHERiPTHR10630 PTHR10630, 1 hit
PIRSFiPIRSF002017 CD14, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCD14_MOUSE
AccessioniPrimary (citable) accession number: P10810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 12, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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