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Protein

S-formylglutathione hydrolase

Gene

ESD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde.2 Publications

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.1 Publication
4-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149Charge relay system1 Publication1
Active sitei226Charge relay system1 Publication1
Active sitei260Charge relay system1 Publication1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB
  • hydrolase activity, acting on ester bonds Source: UniProtKB
  • identical protein binding Source: IntAct
  • methylumbelliferyl-acetate deacetylase activity Source: UniProtKB-EC
  • S-formylglutathione hydrolase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-HSA-156590 Glutathione conjugation

Protein family/group databases

ESTHERihuman-ESD A85-EsteraseD-FGH
MEROPSiS09.990

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase (EC:3.1.2.12)
Short name:
FGH
Alternative name(s):
Esterase D
Methylumbelliferyl-acetate deacetylase (EC:3.1.1.56)
Gene namesi
Name:ESD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000139684.13
HGNCiHGNC:3465 ESD
MIMi133280 gene
neXtProtiNX_P10768

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54L → A: 83% of wild-type activity. 1 Publication1
Mutagenesisi149S → A: Loss of activity. 1 Publication1
Mutagenesisi149S → T: 1.3% of wild-type activity. 1 Publication1
Mutagenesisi150M → A: 62% increase in activity. 1 Publication1
Mutagenesisi226D → A: 4.3% of wild-type activity. 1 Publication1
Mutagenesisi226D → N: 9% of wild-type activity. 1 Publication1
Mutagenesisi260H → A: 3% of wild-type activity. 1 Publication1
Mutagenesisi260H → Q: 1.3% of wild-type activity. 1 Publication1

Organism-specific databases

DisGeNETi2098
OpenTargetsiENSG00000139684
PharmGKBiPA27882

Chemistry databases

ChEMBLiCHEMBL2189130
DrugBankiDB00143 Glutathione

Polymorphism and mutation databases

BioMutaiESD
DMDMi544254

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002103392 – 282S-formylglutathione hydrolaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4N6-succinyllysineBy similarity1
Modified residuei200N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP10768
MaxQBiP10768
PaxDbiP10768
PeptideAtlasiP10768
PRIDEiP10768
ProteomicsDBi52652
TopDownProteomicsiP10768

2D gel databases

DOSAC-COBS-2DPAGEiP10768
OGPiP10768
REPRODUCTION-2DPAGEiIPI00411706
UCD-2DPAGEiP10768

PTM databases

iPTMnetiP10768
PhosphoSitePlusiP10768
SwissPalmiP10768

Expressioni

Gene expression databases

BgeeiENSG00000139684 Expressed in 236 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_ESD
ExpressionAtlasiP10768 baseline and differential
GenevisibleiP10768 HS

Organism-specific databases

HPAiHPA039700

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108402, 31 interactors
IntActiP10768, 9 interactors
STRINGi9606.ENSP00000367992

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10768
SMRiP10768
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10768

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiKOG3101 Eukaryota
COG0627 LUCA
GeneTreeiENSGT00390000011864
HOGENOMiHOG000263929
HOVERGENiHBG001326
InParanoidiP10768
KOiK01070
OMAiEDHLRHH
OrthoDBiEOG091G0FIA
PhylomeDBiP10768
TreeFamiTF300793

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P10768-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALKQISSNK CFGGLQKVFE HDSVELNCKM KFAVYLPPKA ETGKCPALYW
60 70 80 90 100
LSGLTCTEQN FISKSGYHQS ASEHGLVVIA PDTSPRGCNI KGEDESWDFG
110 120 130 140 150
TGAGFYVDAT EDPWKTNYRM YSYVTEELPQ LINANFPVDP QRMSIFGHSM
160 170 180 190 200
GGHGALICAL KNPGKYKSVS AFAPICNPVL CPWGKKAFSG YLGTDQSKWK
210 220 230 240 250
AYDATHLVKS YPGSQLDILI DQGKDDQFLL DGQLLPDNFI AACTEKKIPV
260 270 280
VFRLQEGYDH SYYFIATFIT DHIRHHAKYL NA
Length:282
Mass (Da):31,463
Last modified:June 1, 1994 - v2
Checksum:iBFC20D5FA2BB0DCE
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X6RA14X6RA14_HUMAN
S-formylglutathione hydrolase
ESD
253Annotation score:
H7BZT7H7BZT7_HUMAN
S-formylglutathione hydrolase
ESD
230Annotation score:
U3KQT1U3KQT1_HUMAN
S-formylglutathione hydrolase
ESD
120Annotation score:

Polymorphismi

There are two major electrophoretic isotypes. The sequence of the ESD*1 variant is shown (PubMed:12721789, PubMed:7907313).2 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_005202190G → E in allele ESD*2. 2 PublicationsCorresponds to variant dbSNP:rs9778Ensembl.1
Natural variantiVAR_022275257G → D2 PublicationsCorresponds to variant dbSNP:rs15303Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13450 mRNA Translation: AAA52408.1 Sequence problems.
AF112219 mRNA Translation: AAC99788.1
BT007059 mRNA Translation: AAP35708.1
AL136958 Genomic DNA No translation available.
BC001169 mRNA Translation: AAH01169.1
AF052509 Genomic DNA Translation: AAC06298.1
CCDSiCCDS9404.1
PIRiA23543
RefSeqiNP_001975.1, NM_001984.1
XP_005266335.1, XM_005266278.2
XP_011533256.1, XM_011534954.1
UniGeneiHs.432491

Genome annotation databases

EnsembliENST00000378720; ENSP00000367992; ENSG00000139684
GeneIDi2098
KEGGihsa:2098
UCSCiuc001vbn.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13450 mRNA Translation: AAA52408.1 Sequence problems.
AF112219 mRNA Translation: AAC99788.1
BT007059 mRNA Translation: AAP35708.1
AL136958 Genomic DNA No translation available.
BC001169 mRNA Translation: AAH01169.1
AF052509 Genomic DNA Translation: AAC06298.1
CCDSiCCDS9404.1
PIRiA23543
RefSeqiNP_001975.1, NM_001984.1
XP_005266335.1, XM_005266278.2
XP_011533256.1, XM_011534954.1
UniGeneiHs.432491

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FCXX-ray1.50A/B1-282[»]
ProteinModelPortaliP10768
SMRiP10768
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108402, 31 interactors
IntActiP10768, 9 interactors
STRINGi9606.ENSP00000367992

Chemistry databases

ChEMBLiCHEMBL2189130
DrugBankiDB00143 Glutathione

Protein family/group databases

ESTHERihuman-ESD A85-EsteraseD-FGH
MEROPSiS09.990

PTM databases

iPTMnetiP10768
PhosphoSitePlusiP10768
SwissPalmiP10768

Polymorphism and mutation databases

BioMutaiESD
DMDMi544254

2D gel databases

DOSAC-COBS-2DPAGEiP10768
OGPiP10768
REPRODUCTION-2DPAGEiIPI00411706
UCD-2DPAGEiP10768

Proteomic databases

EPDiP10768
MaxQBiP10768
PaxDbiP10768
PeptideAtlasiP10768
PRIDEiP10768
ProteomicsDBi52652
TopDownProteomicsiP10768

Protocols and materials databases

DNASUi2098
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378720; ENSP00000367992; ENSG00000139684
GeneIDi2098
KEGGihsa:2098
UCSCiuc001vbn.3 human

Organism-specific databases

CTDi2098
DisGeNETi2098
EuPathDBiHostDB:ENSG00000139684.13
GeneCardsiESD
HGNCiHGNC:3465 ESD
HPAiHPA039700
MIMi133280 gene
neXtProtiNX_P10768
OpenTargetsiENSG00000139684
PharmGKBiPA27882
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3101 Eukaryota
COG0627 LUCA
GeneTreeiENSGT00390000011864
HOGENOMiHOG000263929
HOVERGENiHBG001326
InParanoidiP10768
KOiK01070
OMAiEDHLRHH
OrthoDBiEOG091G0FIA
PhylomeDBiP10768
TreeFamiTF300793

Enzyme and pathway databases

ReactomeiR-HSA-156590 Glutathione conjugation

Miscellaneous databases

ChiTaRSiESD human
EvolutionaryTraceiP10768
GeneWikiiESD_(gene)
GenomeRNAii2098
PROiPR:P10768
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139684 Expressed in 236 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_ESD
ExpressionAtlasiP10768 baseline and differential
GenevisibleiP10768 HS

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR014186 S-formylglutathione_hydrol
PANTHERiPTHR10061 PTHR10061, 1 hit
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR02821 fghA_ester_D, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiESTD_HUMAN
AccessioniPrimary (citable) accession number: P10768
Secondary accession number(s): Q5TBU8
, Q5TBV0, Q5TBV2, Q9BVJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 1, 1994
Last modified: September 12, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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