UniProtKB - P10768 (ESTD_HUMAN)
Protein
S-formylglutathione hydrolase
Gene
ESD
Organism
Homo sapiens (Human)
Status
Functioni
Serine hydrolase involved in the detoxification of formaldehyde.2 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 149 | Charge relay system1 Publication | 1 | |
Active sitei | 226 | Charge relay system1 Publication | 1 | |
Active sitei | 260 | Charge relay system1 Publication | 1 |
GO - Molecular functioni
- carboxylic ester hydrolase activity Source: UniProtKB
- hydrolase activity, acting on ester bonds Source: UniProtKB
- identical protein binding Source: IntAct
- methylumbelliferyl-acetate deacetylase activity Source: UniProtKB-EC
- S-formylglutathione hydrolase activity Source: GO_Central
GO - Biological processi
- formaldehyde catabolic process Source: InterPro
- glutathione derivative biosynthetic process Source: Reactome
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Enzyme and pathway databases
PathwayCommonsi | P10768 |
Reactomei | R-HSA-156590, Glutathione conjugation |
Protein family/group databases
ESTHERi | human-ESD, A85-EsteraseD-FGH |
MEROPSi | S09.990 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ESD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3465, ESD |
MIMi | 133280, gene |
neXtProti | NX_P10768 |
VEuPathDBi | HostDB:ENSG00000139684.13 |
Subcellular locationi
Cytoplasm and Cytosol
Other locations
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasmic vesicle Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesiclePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | L → A: 83% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 149 | S → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 149 | S → T: 1.3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 150 | M → A: 62% increase in activity. 1 Publication | 1 | |
Mutagenesisi | 226 | D → A: 4.3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 226 | D → N: 9% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 260 | H → A: 3% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 260 | H → Q: 1.3% of wild-type activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 2098 |
OpenTargetsi | ENSG00000139684 |
PharmGKBi | PA27882 |
Miscellaneous databases
Pharosi | P10768, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2189130 |
DrugBanki | DB00143, Glutathione |
Genetic variation databases
BioMutai | ESD |
DMDMi | 544254 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000210339 | 2 – 282 | S-formylglutathione hydrolaseAdd BLAST | 281 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 4 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 200 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
CPTACi | CPTAC-73 CPTAC-74 |
EPDi | P10768 |
jPOSTi | P10768 |
MassIVEi | P10768 |
MaxQBi | P10768 |
PaxDbi | P10768 |
PeptideAtlasi | P10768 |
PRIDEi | P10768 |
ProteomicsDBi | 52652 |
TopDownProteomicsi | P10768 |
2D gel databases
DOSAC-COBS-2DPAGEi | P10768 |
OGPi | P10768 |
REPRODUCTION-2DPAGEi | IPI00411706 |
UCD-2DPAGEi | P10768 |
PTM databases
iPTMneti | P10768 |
MetOSitei | P10768 |
PhosphoSitePlusi | P10768 |
SwissPalmi | P10768 |
Expressioni
Gene expression databases
Bgeei | ENSG00000139684, Expressed in thoracic mammary gland and 251 other tissues |
ExpressionAtlasi | P10768, baseline and differential |
Genevisiblei | P10768, HS |
Organism-specific databases
HPAi | ENSG00000139684, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
Hide detailsP10768
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-1052334,EBI-1052334 |
FSD2 [A1L4K1] | 7 | EBI-1052334,EBI-5661036 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 108402, 36 interactors |
IntActi | P10768, 11 interactors |
STRINGi | 9606.ENSP00000367992 |
Miscellaneous databases
RNActi | P10768, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10768 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10768 |
Family & Domainsi
Sequence similaritiesi
Belongs to the esterase D family.Curated
Phylogenomic databases
eggNOGi | KOG3101, Eukaryota |
GeneTreei | ENSGT00390000011864 |
InParanoidi | P10768 |
OMAi | QKAFAGY |
OrthoDBi | 942551at2759 |
PhylomeDBi | P10768 |
TreeFami | TF300793 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase_put IPR014186, S-formylglutathione_hydrol |
PANTHERi | PTHR10061, PTHR10061, 1 hit |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR02821, fghA_ester_D, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P10768-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALKQISSNK CFGGLQKVFE HDSVELNCKM KFAVYLPPKA ETGKCPALYW
60 70 80 90 100
LSGLTCTEQN FISKSGYHQS ASEHGLVVIA PDTSPRGCNI KGEDESWDFG
110 120 130 140 150
TGAGFYVDAT EDPWKTNYRM YSYVTEELPQ LINANFPVDP QRMSIFGHSM
160 170 180 190 200
GGHGALICAL KNPGKYKSVS AFAPICNPVL CPWGKKAFSG YLGTDQSKWK
210 220 230 240 250
AYDATHLVKS YPGSQLDILI DQGKDDQFLL DGQLLPDNFI AACTEKKIPV
260 270 280
VFRLQEGYDH SYYFIATFIT DHIRHHAKYL NA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketX6RA14 | X6RA14_HUMAN | S-formylglutathione hydrolase | ESD | 253 | Annotation score: | ||
H7BZT7 | H7BZT7_HUMAN | S-formylglutathione hydrolase | ESD | 230 | Annotation score: | ||
U3KQT1 | U3KQT1_HUMAN | S-formylglutathione hydrolase | ESD hCG_29992 | 262 | Annotation score: |
Polymorphismi
There are two major electrophoretic isotypes. The sequence of the ESD*1 variant is shown (PubMed:12721789, PubMed:7907313).2 Publications
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_005202 | 190 | G → E in allele ESD*2. 2 PublicationsCorresponds to variant dbSNP:rs9778Ensembl. | 1 | |
Natural variantiVAR_022275 | 257 | G → D2 PublicationsCorresponds to variant dbSNP:rs15303Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13450 mRNA Translation: AAA52408.1 Sequence problems. AF112219 mRNA Translation: AAC99788.1 BT007059 mRNA Translation: AAP35708.1 AL136958 Genomic DNA No translation available. BC001169 mRNA Translation: AAH01169.1 AF052509 Genomic DNA Translation: AAC06298.1 |
CCDSi | CCDS9404.1 |
PIRi | A23543 |
RefSeqi | NP_001975.1, NM_001984.1 XP_005266335.1, XM_005266278.2 XP_011533256.1, XM_011534954.1 |
Genome annotation databases
Ensembli | ENST00000378720; ENSP00000367992; ENSG00000139684 |
GeneIDi | 2098 |
KEGGi | hsa:2098 |
UCSCi | uc001vbn.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13450 mRNA Translation: AAA52408.1 Sequence problems. AF112219 mRNA Translation: AAC99788.1 BT007059 mRNA Translation: AAP35708.1 AL136958 Genomic DNA No translation available. BC001169 mRNA Translation: AAH01169.1 AF052509 Genomic DNA Translation: AAC06298.1 |
CCDSi | CCDS9404.1 |
PIRi | A23543 |
RefSeqi | NP_001975.1, NM_001984.1 XP_005266335.1, XM_005266278.2 XP_011533256.1, XM_011534954.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FCX | X-ray | 1.50 | A/B | 1-282 | [»] | |
SMRi | P10768 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108402, 36 interactors |
IntActi | P10768, 11 interactors |
STRINGi | 9606.ENSP00000367992 |
Chemistry databases
ChEMBLi | CHEMBL2189130 |
DrugBanki | DB00143, Glutathione |
Protein family/group databases
ESTHERi | human-ESD, A85-EsteraseD-FGH |
MEROPSi | S09.990 |
PTM databases
iPTMneti | P10768 |
MetOSitei | P10768 |
PhosphoSitePlusi | P10768 |
SwissPalmi | P10768 |
Genetic variation databases
BioMutai | ESD |
DMDMi | 544254 |
2D gel databases
DOSAC-COBS-2DPAGEi | P10768 |
OGPi | P10768 |
REPRODUCTION-2DPAGEi | IPI00411706 |
UCD-2DPAGEi | P10768 |
Proteomic databases
CPTACi | CPTAC-73 CPTAC-74 |
EPDi | P10768 |
jPOSTi | P10768 |
MassIVEi | P10768 |
MaxQBi | P10768 |
PaxDbi | P10768 |
PeptideAtlasi | P10768 |
PRIDEi | P10768 |
ProteomicsDBi | 52652 |
TopDownProteomicsi | P10768 |
Protocols and materials databases
Antibodypediai | 23747, 216 antibodies |
DNASUi | 2098 |
Genome annotation databases
Ensembli | ENST00000378720; ENSP00000367992; ENSG00000139684 |
GeneIDi | 2098 |
KEGGi | hsa:2098 |
UCSCi | uc001vbn.3, human |
Organism-specific databases
CTDi | 2098 |
DisGeNETi | 2098 |
GeneCardsi | ESD |
HGNCi | HGNC:3465, ESD |
HPAi | ENSG00000139684, Low tissue specificity |
MIMi | 133280, gene |
neXtProti | NX_P10768 |
OpenTargetsi | ENSG00000139684 |
PharmGKBi | PA27882 |
VEuPathDBi | HostDB:ENSG00000139684.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3101, Eukaryota |
GeneTreei | ENSGT00390000011864 |
InParanoidi | P10768 |
OMAi | QKAFAGY |
OrthoDBi | 942551at2759 |
PhylomeDBi | P10768 |
TreeFami | TF300793 |
Enzyme and pathway databases
PathwayCommonsi | P10768 |
Reactomei | R-HSA-156590, Glutathione conjugation |
Miscellaneous databases
BioGRID-ORCSi | 2098, 8 hits in 997 CRISPR screens |
ChiTaRSi | ESD, human |
EvolutionaryTracei | P10768 |
GeneWikii | ESD_(gene) |
GenomeRNAii | 2098 |
Pharosi | P10768, Tbio |
PROi | PR:P10768 |
RNActi | P10768, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000139684, Expressed in thoracic mammary gland and 251 other tissues |
ExpressionAtlasi | P10768, baseline and differential |
Genevisiblei | P10768, HS |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase_put IPR014186, S-formylglutathione_hydrol |
PANTHERi | PTHR10061, PTHR10061, 1 hit |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR02821, fghA_ester_D, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ESTD_HUMAN | |
Accessioni | P10768Primary (citable) accession number: P10768 Secondary accession number(s): Q5TBU8 Q9BVJ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | June 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families