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Protein

Zona pellucida sperm-binding protein 3

Gene

Zp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

  • acrosin binding Source: MGI
  • carbohydrate binding Source: UniProtKB
  • identical protein binding Source: MGI
  • receptor ligand activity Source: UniProtKB
  • structural constituent of egg coat Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionReceptor

Enzyme and pathway databases

ReactomeiR-MMU-1300644 Interaction With The Zona Pellucida

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:Zp3
Synonyms:Zp-3, Zpc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:99215 Zp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 387ExtracellularAdd BLAST365
Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
Topological domaini409 – 424CytoplasmicAdd BLAST16

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi111Y → C: Reduces protein solubility and causes formation of an abnormal disulfide bond between C-111 and C-139 in approximately half of the molecules. 1 Publication1
Mutagenesisi111Y → F: Causes a mild reduction of protein solubility. 1 Publication1
Mutagenesisi111Y → L or V: Strongly reduces protein solubility. 1 Publication1
Mutagenesisi111Y → S: Reduces protein solubility. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000004171523 – 351Zona pellucida sperm-binding protein 3Add BLAST329
ChainiPRO_000030457223 – ?Processed zona pellucida sperm-binding protein 3
PropeptideiPRO_0000041716352 – 424Removed in mature form1 PublicationAdd BLAST73

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Pyrrolidone carboxylic acid1 Publication1
Glycosylationi32O-linked (GalNAc...) threonineCurated1
Glycosylationi34O-linked (GalNAc...) threonineCurated1
Glycosylationi39O-linked (GalNAc...) serineCurated1
Disulfide bondi46 ↔ 1391 Publication
Disulfide bondi78 ↔ 981 Publication
Glycosylationi146N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi155O-linked (GalNAc...) threonineCurated1
Glycosylationi162O-linked (GalNAc...) threonineCurated1
Disulfide bondi216 ↔ 2831 Publication
Disulfide bondi240 ↔ 3011 Publication
Glycosylationi273N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi304N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi327N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi330N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.1 Publication
Cys-320, Cys-322, Cys-323 and Cys-328 are involved in two additional disulfide bonds.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP10761
PRIDEiP10761

PTM databases

GlyConnecti633
635
iPTMnetiP10761
PhosphoSitePlusiP10761
UniCarbKBiP10761

Expressioni

Tissue specificityi

Oocytes.

Developmental stagei

Expressed during the 2-week growth phase of oogenesis, prior to ovulation.

Gene expression databases

BgeeiENSMUSG00000004948 Expressed in 31 organ(s), highest expression level in primary oocyte
CleanExiMM_ZP3
ExpressionAtlasiP10761 baseline and differential
GenevisibleiP10761 MM

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers. Interacts with ZP1 and ZP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005073

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10761
SMRiP10761
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10761

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 308ZPPROSITE-ProRule annotationAdd BLAST264

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9V Eukaryota
ENOG410YXE1 LUCA
GeneTreeiENSGT00530000063482
HOGENOMiHOG000220813
HOVERGENiHBG007985
InParanoidiP10761
KOiK19928
OMAiYRLFICL
OrthoDBiEOG091G0AQ6
PhylomeDBiP10761
TreeFamiTF331369

Family and domain databases

InterProiView protein in InterPro
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P10761-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSYFLFLC LLLCGGPELC NSQTLWLLPG GTPTPVGSSS PVKVECLEAE
60 70 80 90 100
LVVTVSRDLF GTGKLVQPGD LTLGSEGCQP RVSVDTDVVR FNAQLHECSS
110 120 130 140 150
RVQMTKDALV YSTFLLHDPR PVSGLSILRT NRVEVPIECR YPRQGNVSSH
160 170 180 190 200
PIQPTWVPFR ATVSSEEKLA FSLRLMEENW NTEKSAPTFH LGEVAHLQAE
210 220 230 240 250
VQTGSHLPLQ LFVDHCVATP SPLPDPNSSP YHFIVDFHGC LVDGLSESFS
260 270 280 290 300
AFQVPRPRPE TLQFTVDVFH FANSSRNTLY ITCHLKVAPA NQIPDKLNKA
310 320 330 340 350
CSFNKTSQSW LPVEGDADIC DCCSHGNCSN SSSSQFQIHG PRQWSKLVSR
360 370 380 390 400
NRRHVTDEAD VTVGPLIFLG KANDQTVEGW TASAQTSVAL GLGLATVAFL
410 420
TLAAIVLAVT RKCHSSSYLV SLPQ
Length:424
Mass (Da):46,304
Last modified:November 1, 1997 - v4
Checksum:i9089903FBD268365
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6VD35F6VD35_MOUSE
Zona pellucida sperm-binding protei...
Zp3
136Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20026 mRNA Translation: AAB18629.1
X14376 Genomic DNA Translation: CAA32550.1
BC099465 mRNA Translation: AAH99465.1
BC100745 mRNA Translation: AAI00746.1
BC103583 mRNA Translation: AAI03584.1
BC103584 mRNA Translation: AAI03585.1
BC103585 mRNA Translation: AAI03586.1
CCDSiCCDS19749.1
PIRiA30334
RefSeqiNP_035906.1, NM_011776.1
UniGeneiMm.1381

Genome annotation databases

EnsembliENSMUST00000005073; ENSMUSP00000005073; ENSMUSG00000004948
GeneIDi22788
KEGGimmu:22788
UCSCiuc008zzl.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20026 mRNA Translation: AAB18629.1
X14376 Genomic DNA Translation: CAA32550.1
BC099465 mRNA Translation: AAH99465.1
BC100745 mRNA Translation: AAI00746.1
BC103583 mRNA Translation: AAI03584.1
BC103584 mRNA Translation: AAI03585.1
BC103585 mRNA Translation: AAI03586.1
CCDSiCCDS19749.1
PIRiA30334
RefSeqiNP_035906.1, NM_011776.1
UniGeneiMm.1381

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D4CX-ray2.90A42-143[»]
3D4GX-ray2.30A/B/C/D/E/F/G/H42-143[»]
3EF7X-ray3.10A/B42-143[»]
5OSQX-ray2.05A/B42-143[»]
ProteinModelPortaliP10761
SMRiP10761
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005073

PTM databases

GlyConnecti633
635
iPTMnetiP10761
PhosphoSitePlusiP10761
UniCarbKBiP10761

Proteomic databases

PaxDbiP10761
PRIDEiP10761

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005073; ENSMUSP00000005073; ENSMUSG00000004948
GeneIDi22788
KEGGimmu:22788
UCSCiuc008zzl.1 mouse

Organism-specific databases

CTDi7784
MGIiMGI:99215 Zp3

Phylogenomic databases

eggNOGiENOG410II9V Eukaryota
ENOG410YXE1 LUCA
GeneTreeiENSGT00530000063482
HOGENOMiHOG000220813
HOVERGENiHBG007985
InParanoidiP10761
KOiK19928
OMAiYRLFICL
OrthoDBiEOG091G0AQ6
PhylomeDBiP10761
TreeFamiTF331369

Enzyme and pathway databases

ReactomeiR-MMU-1300644 Interaction With The Zona Pellucida

Miscellaneous databases

EvolutionaryTraceiP10761
PROiPR:P10761
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004948 Expressed in 31 organ(s), highest expression level in primary oocyte
CleanExiMM_ZP3
ExpressionAtlasiP10761 baseline and differential
GenevisibleiP10761 MM

Family and domain databases

InterProiView protein in InterPro
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZP3_MOUSE
AccessioniPrimary (citable) accession number: P10761
Secondary accession number(s): Q4FZI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 154 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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Main funding by: National Institutes of Health

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