UniProtKB - P10749 (IL1B_MOUSE)
Interleukin-1 beta
Il1b
Functioni
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
By similarityMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 172 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 180 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 182 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 191 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 205 | Important for interaction with integrinBy similarity | 1 |
GO - Molecular functioni
- cytokine activity Source: MGI
- integrin binding Source: UniProtKB
- interleukin-1 receptor binding Source: InterPro
- protein domain specific binding Source: MGI
GO - Biological processi
- astrocyte activation Source: MGI
- cellular response to lipopolysaccharide Source: GO_Central
- cellular response to mechanical stimulus Source: Ensembl
- cellular response to organic cyclic compound Source: MGI
- cellular response to organic substance Source: MGI
- cellular response to xenobiotic stimulus Source: MGI
- cytokine-mediated signaling pathway Source: MGI
- ectopic germ cell programmed cell death Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- fever generation Source: UniProtKB-KW
- hyaluronan biosynthetic process Source: MGI
- immune response Source: InterPro
- inflammatory response Source: MGI
- interleukin-1-mediated signaling pathway Source: MGI
- leukocyte migration Source: MGI
- lipopolysaccharide-mediated signaling pathway Source: MGI
- MAPK cascade Source: MGI
- memory Source: MGI
- monocyte aggregation Source: MGI
- negative regulation of adiponectin secretion Source: BHF-UCL
- negative regulation of branching morphogenesis of a nerve Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of gap junction assembly Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of glucose transmembrane transport Source: BHF-UCL
- negative regulation of glutamate secretion Source: MGI
- negative regulation of insulin receptor signaling pathway Source: BHF-UCL
- negative regulation of lipid catabolic process Source: MGI
- negative regulation of lipid metabolic process Source: BHF-UCL
- negative regulation of MAP kinase activity Source: BHF-UCL
- negative regulation of neural precursor cell proliferation Source: MGI
- negative regulation of neurogenesis Source: MGI
- negative regulation of neuron differentiation Source: MGI
- negative regulation of synaptic transmission Source: ARUK-UCL
- negative regulation of transcription by RNA polymerase II Source: MGI
- neutrophil chemotaxis Source: MGI
- positive regulation of angiogenesis Source: BHF-UCL
- positive regulation of apoptotic process Source: MGI
- positive regulation of astrocyte differentiation Source: MGI
- positive regulation of calcidiol 1-monooxygenase activity Source: MGI
- positive regulation of cell death Source: MGI
- positive regulation of cell division Source: UniProtKB-KW
- positive regulation of cell migration Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of chemokine production Source: MGI
- positive regulation of complement activation Source: MGI
- positive regulation of cytosolic calcium ion concentration Source: MGI
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of epithelial to mesenchymal transition Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of fever generation Source: BHF-UCL
- positive regulation of gene expression Source: MGI
- positive regulation of glial cell differentiation Source: MGI
- positive regulation of glial cell proliferation Source: MGI
- positive regulation of granulocyte macrophage colony-stimulating factor production Source: MGI
- positive regulation of heterotypic cell-cell adhesion Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- positive regulation of immature T cell proliferation in thymus Source: MGI
- positive regulation of inflammatory response Source: MGI
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of interleukin-2 production Source: MGI
- positive regulation of interleukin-6 production Source: UniProtKB
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of JNK cascade Source: MGI
- positive regulation of JUN kinase activity Source: MGI
- positive regulation of lipid catabolic process Source: BHF-UCL
- positive regulation of MAP kinase activity Source: MGI
- positive regulation of membrane protein ectodomain proteolysis Source: MGI
- positive regulation of mitotic nuclear division Source: MGI
- positive regulation of monocyte chemotactic protein-1 production Source: MGI
- positive regulation of myosin light chain kinase activity Source: MGI
- positive regulation of neuron apoptotic process Source: MGI
- positive regulation of neutrophil chemotaxis Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of p38MAPK cascade Source: ARUK-UCL
- positive regulation of phagocytosis Source: MGI
- positive regulation of prostaglandin biosynthetic process Source: MGI
- positive regulation of prostaglandin secretion Source: BHF-UCL
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of RNA biosynthetic process Source: MGI
- positive regulation of stress-activated MAPK cascade Source: MGI
- positive regulation of T cell proliferation Source: MGI
- positive regulation of T-helper 1 cell cytokine production Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of vascular endothelial growth factor production Source: BHF-UCL
- protein kinase B signaling Source: MGI
- regulation of defense response to virus by host Source: CAFA
- regulation of ERK1 and ERK2 cascade Source: MGI
- regulation of establishment of endothelial barrier Source: MGI
- regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- regulation of insulin secretion Source: MGI
- regulation of neurogenesis Source: MGI
- regulation of nitric-oxide synthase activity Source: UniProtKB
- response to ATP Source: MGI
- response to carbohydrate Source: MGI
- response to interleukin-1 Source: MGI
- response to lipopolysaccharide Source: MGI
- sequestering of triglyceride Source: MGI
- social behavior Source: MGI
- vascular endothelial growth factor production Source: UniProtKB
Keywordsi
Molecular function | Cytokine, Mitogen, Pyrogen |
Biological process | Inflammatory response |
Enzyme and pathway databases
Reactomei | R-MMU-448706, Interleukin-1 processing R-MMU-5620971, Pyroptosis R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-9020702, Interleukin-1 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin-1 betaShort name: IL-1 beta |
Gene namesi | Name:Il1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96543, Il1b |
VEuPathDBi | HostDB:ENSMUSG00000027398 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Extracellular region or secreted
- Secreted 2 Publications
- extracellular exosome 1 Publication
Lysosome
- Lysosome By similarity
Note: The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10.By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: CAFA
Lysosome
- lysosome Source: UniProtKB-SubCell
Other locations
- autophagosome Source: UniProtKB-SubCell
- secretory granule Source: MGI
- vesicle Source: MGI
Keywords - Cellular componenti
Cytoplasm, Lysosome, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000015311 | 1 – 117 | 1 PublicationAdd BLAST | 117 | |
ChainiPRO_0000015312 | 118 – 269 | Interleukin-1 betaAdd BLAST | 152 |
Proteomic databases
PaxDbi | P10749 |
PRIDEi | P10749 |
ProteomicsDBi | 266968 |
TopDownProteomicsi | P10749 |
PTM databases
iPTMneti | P10749 |
PhosphoSitePlusi | P10749 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027398, Expressed in granulocyte and 117 other tissues |
Genevisiblei | P10749, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with MEFV.
Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling.
Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (By similarity).
Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (By similarity).
Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: MGI
- integrin binding Source: UniProtKB
- interleukin-1 receptor binding Source: InterPro
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200624, 8 interactors |
IntActi | P10749, 1 interactor |
STRINGi | 10090.ENSMUSP00000028881 |
Miscellaneous databases
RNActi | P10749, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P10749 |
SMRi | P10749 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10749 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S3E9, Eukaryota |
GeneTreei | ENSGT00950000182943 |
HOGENOMi | CLU_083639_0_0_1 |
InParanoidi | P10749 |
OMAi | QHYSKGF |
OrthoDBi | 1243742at2759 |
PhylomeDBi | P10749 |
TreeFami | TF300203 |
Family and domain databases
InterProi | View protein in InterPro IPR003296, IL-1_beta IPR020877, IL-1_CS IPR000975, IL-1_fam IPR003502, IL-1_propep IPR008996, IL1/FGF |
PANTHERi | PTHR10078, PTHR10078, 1 hit PTHR10078:SF30, PTHR10078:SF30, 1 hit |
Pfami | View protein in Pfam PF00340, IL1, 1 hit PF02394, IL1_propep, 1 hit |
PRINTSi | PR00264, INTERLEUKIN1 |
SUPFAMi | SSF50353, SSF50353, 1 hit |
PROSITEi | View protein in PROSITE PS00253, INTERLEUKIN_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ
60 70 80 90 100
ISQQHINKSF RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE
110 120 130 140 150
PILCDSWDDD DNLLVCDVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG
160 170 180 190 200
QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE
210 220 230 240 250
SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF
260
LGNNSGQDII DFTMESVSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15131 mRNA Translation: AAA39276.1 X04964 Genomic DNA Translation: CAA28637.1 AY902319 Genomic DNA Translation: AAX90604.1 CH466519 Genomic DNA Translation: EDL28238.1 BC011437 mRNA Translation: AAH11437.1 |
CCDSi | CCDS16726.1 |
PIRi | I55969 |
RefSeqi | NP_032387.1, NM_008361.4 |
Genome annotation databases
Ensembli | ENSMUST00000028881; ENSMUSP00000028881; ENSMUSG00000027398 |
GeneIDi | 16176 |
KEGGi | mmu:16176 |
UCSCi | uc008mht.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15131 mRNA Translation: AAA39276.1 X04964 Genomic DNA Translation: CAA28637.1 AY902319 Genomic DNA Translation: AAX90604.1 CH466519 Genomic DNA Translation: EDL28238.1 BC011437 mRNA Translation: AAH11437.1 |
CCDSi | CCDS16726.1 |
PIRi | I55969 |
RefSeqi | NP_032387.1, NM_008361.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MIB | X-ray | 2.84 | A | 118-269 | [»] | |
8I1B | X-ray | 2.40 | A | 118-269 | [»] | |
AlphaFoldDBi | P10749 | |||||
SMRi | P10749 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200624, 8 interactors |
IntActi | P10749, 1 interactor |
STRINGi | 10090.ENSMUSP00000028881 |
PTM databases
iPTMneti | P10749 |
PhosphoSitePlusi | P10749 |
Proteomic databases
PaxDbi | P10749 |
PRIDEi | P10749 |
ProteomicsDBi | 266968 |
TopDownProteomicsi | P10749 |
Protocols and materials databases
ABCDi | P10749, 1 sequenced antibody |
Antibodypediai | 771, 2434 antibodies from 49 providers |
DNASUi | 16176 |
Genome annotation databases
Ensembli | ENSMUST00000028881; ENSMUSP00000028881; ENSMUSG00000027398 |
GeneIDi | 16176 |
KEGGi | mmu:16176 |
UCSCi | uc008mht.1, mouse |
Organism-specific databases
CTDi | 3553 |
MGIi | MGI:96543, Il1b |
VEuPathDBi | HostDB:ENSMUSG00000027398 |
Phylogenomic databases
eggNOGi | ENOG502S3E9, Eukaryota |
GeneTreei | ENSGT00950000182943 |
HOGENOMi | CLU_083639_0_0_1 |
InParanoidi | P10749 |
OMAi | QHYSKGF |
OrthoDBi | 1243742at2759 |
PhylomeDBi | P10749 |
TreeFami | TF300203 |
Enzyme and pathway databases
Reactomei | R-MMU-448706, Interleukin-1 processing R-MMU-5620971, Pyroptosis R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-9020702, Interleukin-1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 16176, 1 hit in 74 CRISPR screens |
ChiTaRSi | Il1b, mouse |
EvolutionaryTracei | P10749 |
PROi | PR:P10749 |
RNActi | P10749, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027398, Expressed in granulocyte and 117 other tissues |
Genevisiblei | P10749, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003296, IL-1_beta IPR020877, IL-1_CS IPR000975, IL-1_fam IPR003502, IL-1_propep IPR008996, IL1/FGF |
PANTHERi | PTHR10078, PTHR10078, 1 hit PTHR10078:SF30, PTHR10078:SF30, 1 hit |
Pfami | View protein in Pfam PF00340, IL1, 1 hit PF02394, IL1_propep, 1 hit |
PRINTSi | PR00264, INTERLEUKIN1 |
SUPFAMi | SSF50353, SSF50353, 1 hit |
PROSITEi | View protein in PROSITE PS00253, INTERLEUKIN_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IL1B_MOUSE | |
Accessioni | P10749Primary (citable) accession number: P10749 Secondary accession number(s): Q2M4J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | May 25, 2022 | |
This is version 205 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families