UniProtKB - P10749 (IL1B_MOUSE)
Interleukin-1 beta
Il1b
Functioni
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 172 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 180 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 182 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 191 | Important for interaction with integrinBy similarity | 1 | |
Sitei | 205 | Important for interaction with integrinBy similarity | 1 |
GO - Molecular functioni
- cytokine activity Source: MGI
- integrin binding Source: UniProtKB
- interleukin-1 receptor binding Source: InterPro
- protein domain specific binding Source: MGI
GO - Biological processi
- activation of MAPK activity Source: MGI
- astrocyte activation Source: MGI
- cellular response to drug Source: MGI
- cellular response to lipopolysaccharide Source: GO_Central
- cellular response to mechanical stimulus Source: Ensembl
- cellular response to organic cyclic compound Source: MGI
- cellular response to organic substance Source: MGI
- cytokine-mediated signaling pathway Source: MGI
- ectopic germ cell programmed cell death Source: MGI
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- fever generation Source: UniProtKB-KW
- hyaluronan biosynthetic process Source: MGI
- immune response Source: InterPro
- inflammatory response Source: MGI
- interleukin-1 beta production Source: MGI
- interleukin-1-mediated signaling pathway Source: MGI
- interleukin-6 production Source: MGI
- leukocyte migration Source: MGI
- lipopolysaccharide-mediated signaling pathway Source: MGI
- MAPK cascade Source: MGI
- memory Source: MGI
- monocyte aggregation Source: MGI
- negative regulation of adiponectin secretion Source: BHF-UCL
- negative regulation of branching morphogenesis of a nerve Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of gap junction assembly Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of glucose transmembrane transport Source: BHF-UCL
- negative regulation of glutamate secretion Source: MGI
- negative regulation of insulin receptor signaling pathway Source: BHF-UCL
- negative regulation of lipid catabolic process Source: MGI
- negative regulation of lipid metabolic process Source: BHF-UCL
- negative regulation of MAP kinase activity Source: BHF-UCL
- negative regulation of neural precursor cell proliferation Source: MGI
- negative regulation of neurogenesis Source: MGI
- negative regulation of neuron differentiation Source: MGI
- negative regulation of synaptic transmission Source: ARUK-UCL
- negative regulation of transcription by RNA polymerase II Source: MGI
- neutrophil chemotaxis Source: MGI
- positive regulation of angiogenesis Source: BHF-UCL
- positive regulation of apoptotic process Source: MGI
- positive regulation of astrocyte differentiation Source: MGI
- positive regulation of calcidiol 1-monooxygenase activity Source: MGI
- positive regulation of cell death Source: MGI
- positive regulation of cell division Source: UniProtKB-KW
- positive regulation of cell migration Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of chemokine production Source: MGI
- positive regulation of complement activation Source: MGI
- positive regulation of cytosolic calcium ion concentration Source: MGI
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of epithelial to mesenchymal transition Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of fever generation Source: BHF-UCL
- positive regulation of gene expression Source: MGI
- positive regulation of glial cell differentiation Source: MGI
- positive regulation of glial cell proliferation Source: MGI
- positive regulation of granulocyte macrophage colony-stimulating factor production Source: MGI
- positive regulation of heterotypic cell-cell adhesion Source: MGI
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- positive regulation of immature T cell proliferation in thymus Source: MGI
- positive regulation of inflammatory response Source: MGI
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of interleukin-2 production Source: MGI
- positive regulation of interleukin-6 production Source: UniProtKB
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of JNK cascade Source: MGI
- positive regulation of JUN kinase activity Source: MGI
- positive regulation of lipid catabolic process Source: BHF-UCL
- positive regulation of membrane protein ectodomain proteolysis Source: MGI
- positive regulation of mitotic nuclear division Source: MGI
- positive regulation of monocyte chemotactic protein-1 production Source: MGI
- positive regulation of myosin light chain kinase activity Source: MGI
- positive regulation of neuron apoptotic process Source: MGI
- positive regulation of neutrophil chemotaxis Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of nitric oxide biosynthetic process Source: MGI
- positive regulation of p38MAPK cascade Source: ARUK-UCL
- positive regulation of phagocytosis Source: MGI
- positive regulation of prostaglandin biosynthetic process Source: MGI
- positive regulation of prostaglandin secretion Source: BHF-UCL
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of RNA biosynthetic process Source: MGI
- positive regulation of stress-activated MAPK cascade Source: MGI
- positive regulation of T cell proliferation Source: MGI
- positive regulation of T-helper 1 cell cytokine production Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of vascular endothelial growth factor production Source: BHF-UCL
- protein kinase B signaling Source: MGI
- regulation of defense response to virus by host Source: CAFA
- regulation of ERK1 and ERK2 cascade Source: MGI
- regulation of establishment of endothelial barrier Source: MGI
- regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- regulation of insulin secretion Source: MGI
- regulation of neurogenesis Source: MGI
- regulation of nitric-oxide synthase activity Source: UniProtKB
- response to ATP Source: MGI
- response to carbohydrate Source: MGI
- response to interleukin-1 Source: MGI
- response to lipopolysaccharide Source: MGI
- sequestering of triglyceride Source: MGI
- social behavior Source: MGI
- vascular endothelial growth factor production Source: UniProtKB
Keywordsi
Molecular function | Cytokine, Mitogen, Pyrogen |
Biological process | Inflammatory response |
Enzyme and pathway databases
Reactomei | R-MMU-448706, Interleukin-1 processing R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-9020702, Interleukin-1 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin-1 betaShort name: IL-1 beta |
Gene namesi | Name:Il1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96543, Il1b |
Subcellular locationi
Extracellular region or secreted
- extracellular exosome 1 Publication
- Secreted 2 Publications
Cytosol
- cytosol 1 Publication
Lysosome
- Lysosome By similarity
Other locations
- autophagosome By similarity
Note: The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca2+-dependent exocytosis with release of mature IL1B (By similarity). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (By similarity). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (PubMed:17641058). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (By similarity). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (PubMed:17284521). 6. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion, and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9 (By similarity). These mechanisms may not be not mutually exclusive.By similarity2 Publications
Cytosol
- cytosol Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: CAFA
Lysosome
- lysosome Source: UniProtKB-SubCell
Other locations
- autophagosome Source: UniProtKB-SubCell
- secretory granule Source: MGI
- vesicle Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Lysosome, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000015311 | 1 – 117 | 1 PublicationAdd BLAST | 117 | |
ChainiPRO_0000015312 | 118 – 269 | Interleukin-1 betaAdd BLAST | 152 |
Proteomic databases
PaxDbi | P10749 |
PRIDEi | P10749 |
TopDownProteomicsi | P10749 |
PTM databases
iPTMneti | P10749 |
PhosphoSitePlusi | P10749 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000027398, Expressed in granulocyte and 108 other tissues |
Genevisiblei | P10749, MM |
Interactioni
Subunit structurei
Monomer.
Interacts with MEFV.
Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling.
Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (By similarity).
Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (By similarity).
Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: MGI
- integrin binding Source: UniProtKB
- interleukin-1 receptor binding Source: InterPro
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200624, 8 interactors |
IntActi | P10749, 1 interactor |
STRINGi | 10090.ENSMUSP00000028881 |
Miscellaneous databases
RNActi | P10749, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P10749 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10749 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S3E9, Eukaryota |
GeneTreei | ENSGT00950000182943 |
HOGENOMi | CLU_083639_0_0_1 |
InParanoidi | P10749 |
OMAi | RQPVGIT |
OrthoDBi | 1243742at2759 |
PhylomeDBi | P10749 |
TreeFami | TF300203 |
Family and domain databases
InterProi | View protein in InterPro IPR003296, IL-1_beta IPR020877, IL-1_CS IPR000975, IL-1_fam IPR003502, IL-1_propep IPR008996, IL1/FGF |
PANTHERi | PTHR10078, PTHR10078, 1 hit PTHR10078:SF30, PTHR10078:SF30, 1 hit |
Pfami | View protein in Pfam PF00340, IL1, 1 hit PF02394, IL1_propep, 1 hit |
PRINTSi | PR00264, INTERLEUKIN1 |
SUPFAMi | SSF50353, SSF50353, 1 hit |
PROSITEi | View protein in PROSITE PS00253, INTERLEUKIN_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ
60 70 80 90 100
ISQQHINKSF RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE
110 120 130 140 150
PILCDSWDDD DNLLVCDVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG
160 170 180 190 200
QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE
210 220 230 240 250
SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF
260
LGNNSGQDII DFTMESVSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15131 mRNA Translation: AAA39276.1 X04964 Genomic DNA Translation: CAA28637.1 AY902319 Genomic DNA Translation: AAX90604.1 CH466519 Genomic DNA Translation: EDL28238.1 BC011437 mRNA Translation: AAH11437.1 |
CCDSi | CCDS16726.1 |
PIRi | I55969 |
RefSeqi | NP_032387.1, NM_008361.4 |
Genome annotation databases
Ensembli | ENSMUST00000028881; ENSMUSP00000028881; ENSMUSG00000027398 |
GeneIDi | 16176 |
KEGGi | mmu:16176 |
UCSCi | uc008mht.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15131 mRNA Translation: AAA39276.1 X04964 Genomic DNA Translation: CAA28637.1 AY902319 Genomic DNA Translation: AAX90604.1 CH466519 Genomic DNA Translation: EDL28238.1 BC011437 mRNA Translation: AAH11437.1 |
CCDSi | CCDS16726.1 |
PIRi | I55969 |
RefSeqi | NP_032387.1, NM_008361.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MIB | X-ray | 2.84 | A | 118-269 | [»] | |
8I1B | X-ray | 2.40 | A | 118-269 | [»] | |
SMRi | P10749 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200624, 8 interactors |
IntActi | P10749, 1 interactor |
STRINGi | 10090.ENSMUSP00000028881 |
PTM databases
iPTMneti | P10749 |
PhosphoSitePlusi | P10749 |
Proteomic databases
PaxDbi | P10749 |
PRIDEi | P10749 |
TopDownProteomicsi | P10749 |
Protocols and materials databases
Antibodypediai | 771, 2315 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028881; ENSMUSP00000028881; ENSMUSG00000027398 |
GeneIDi | 16176 |
KEGGi | mmu:16176 |
UCSCi | uc008mht.1, mouse |
Organism-specific databases
CTDi | 3553 |
MGIi | MGI:96543, Il1b |
Phylogenomic databases
eggNOGi | ENOG502S3E9, Eukaryota |
GeneTreei | ENSGT00950000182943 |
HOGENOMi | CLU_083639_0_0_1 |
InParanoidi | P10749 |
OMAi | RQPVGIT |
OrthoDBi | 1243742at2759 |
PhylomeDBi | P10749 |
TreeFami | TF300203 |
Enzyme and pathway databases
Reactomei | R-MMU-448706, Interleukin-1 processing R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-9020702, Interleukin-1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 16176, 0 hits in 17 CRISPR screens |
ChiTaRSi | Il1b, mouse |
EvolutionaryTracei | P10749 |
PROi | PR:P10749 |
RNActi | P10749, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027398, Expressed in granulocyte and 108 other tissues |
Genevisiblei | P10749, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003296, IL-1_beta IPR020877, IL-1_CS IPR000975, IL-1_fam IPR003502, IL-1_propep IPR008996, IL1/FGF |
PANTHERi | PTHR10078, PTHR10078, 1 hit PTHR10078:SF30, PTHR10078:SF30, 1 hit |
Pfami | View protein in Pfam PF00340, IL1, 1 hit PF02394, IL1_propep, 1 hit |
PRINTSi | PR00264, INTERLEUKIN1 |
SUPFAMi | SSF50353, SSF50353, 1 hit |
PROSITEi | View protein in PROSITE PS00253, INTERLEUKIN_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IL1B_MOUSE | |
Accessioni | P10749Primary (citable) accession number: P10749 Secondary accession number(s): Q2M4J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 200 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families