UniProtKB - P10747 (CD28_HUMAN)
Protein
T-cell-specific surface glycoprotein CD28
Gene
CD28
Organism
Homo sapiens (Human)
Status
Functioni
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).2 Publications
GO - Molecular functioni
- coreceptor activity Source: UniProtKB
- identical protein binding Source: UniProtKB
- protease binding Source: BHF-UCL
- protein kinase binding Source: Ensembl
GO - Biological processi
- apoptotic signaling pathway Source: Ensembl
- cell surface receptor signaling pathway Source: UniProtKB
- cytokine biosynthetic process Source: UniProtKB
- humoral immune response Source: UniProtKB
- immune system process Source: GO_Central
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of gene expression Source: UniProtKB
- negative thymic T cell selection Source: Ensembl
- positive regulation of alpha-beta T cell proliferation Source: Ensembl
- positive regulation of gene expression Source: UniProtKB
- positive regulation of inflammatory response to antigenic stimulus Source: Ensembl
- positive regulation of interleukin-10 production Source: UniProtKB
- positive regulation of interleukin-2 production Source: UniProtKB
- positive regulation of interleukin-4 production Source: UniProtKB
- positive regulation of isotype switching to IgG isotypes Source: Ensembl
- positive regulation of mitotic nuclear division Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
- positive regulation of protein kinase B signaling Source: Reactome
- positive regulation of T cell proliferation Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of translation Source: UniProtKB
- positive regulation of viral genome replication Source: UniProtKB
- regulation of defense response to virus by virus Source: Reactome
- regulation of regulatory T cell differentiation Source: Ensembl
- regulatory T cell differentiation Source: BHF-UCL
- T cell activation Source: BHF-UCL
- T cell costimulation Source: GO_Central
- T cell receptor signaling pathway Source: GO_Central
Enzyme and pathway databases
PathwayCommonsi | P10747 |
Reactomei | R-HSA-1257604, PIP3 activates AKT signaling R-HSA-164939, Nef mediated downregulation of CD28 cell surface expression R-HSA-2219530, Constitutive Signaling by Aberrant PI3K in Cancer R-HSA-389356, CD28 co-stimulation R-HSA-389357, CD28 dependent PI3K/Akt signaling R-HSA-389359, CD28 dependent Vav1 pathway R-HSA-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling |
SignaLinki | P10747 |
SIGNORi | P10747 |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell-specific surface glycoprotein CD28Alternative name(s): TP44 CD_antigen: CD28 |
Gene namesi | Name:CD28 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000178562.17 |
HGNCi | HGNC:1653, CD28 |
MIMi | 186760, gene |
neXtProti | NX_P10747 |
Subcellular locationi
Other locations
Other locations
- Cell surface 1 Publication
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- external side of plasma membrane Source: MGI
- immunological synapse Source: Ensembl
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cell surface Source: UniProtKB
- protein complex involved in cell adhesion Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 152 | ExtracellularSequence analysisAdd BLAST | 134 | |
Transmembranei | 153 – 179 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 180 – 220 | CytoplasmicSequence analysisAdd BLAST | 41 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 940 |
MalaCardsi | CD28 |
OpenTargetsi | ENSG00000178562 |
Orphaneti | 2584, Classic mycosis fungoides 3162, Sezary syndrome |
PharmGKBi | PA26207 |
Miscellaneous databases
Pharosi | P10747, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5191 |
GuidetoPHARMACOLOGYi | 2863 |
Polymorphism and mutation databases
BioMutai | CD28 |
DMDMi | 115973 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Add BLAST | 18 | |
ChainiPRO_0000014652 | 19 – 220 | T-cell-specific surface glycoprotein CD28Add BLAST | 202 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 40 ↔ 112 | 1 Publication | ||
Disulfide bondi | 66 ↔ 86 | 1 Publication | ||
Glycosylationi | 71 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 105 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 129 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 189 | PhosphoserineCombined sources | 1 | |
Modified residuei | 191 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 209 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
CD40LG induces tyrosine phosphorylation of isoform 3.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MassIVEi | P10747 |
MaxQBi | P10747 |
PaxDbi | P10747 |
PeptideAtlasi | P10747 |
PRIDEi | P10747 |
ProteomicsDBi | 52645 [P10747-1] 52646 [P10747-2] 52647 [P10747-3] 52648 [P10747-4] 52649 [P10747-5] 52650 [P10747-6] 68570 |
PTM databases
GlyGeni | P10747, 5 sites |
iPTMneti | P10747 |
PhosphoSitePlusi | P10747 |
Expressioni
Tissue specificityi
Expressed in T-cells and plasma cells, but not in less mature B-cells.
Gene expression databases
Bgeei | ENSG00000178562, Expressed in leukocyte and 124 other tissues |
Genevisiblei | P10747, HS |
Organism-specific databases
HPAi | ENSG00000178562, Tissue enhanced (blood, lymphoid tissue) |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.
Interacts with GRB2.
Isoform 3 interacts with CD40LG (PubMed:15067037).
3 PublicationsBinary interactionsi
Hide detailsP10747
With | #Exp. | IntAct |
---|---|---|
PIK3R1 [P27986] | 10 | EBI-4314301,EBI-79464 |
Isoform 1 [P10747-1]
With | #Exp. | IntAct |
---|---|---|
entB [P01552] from Staphylococcus aureus. | 5 | EBI-15945088,EBI-1027464 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protease binding Source: BHF-UCL
- protein kinase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 107378, 20 interactors |
CORUMi | P10747 |
DIPi | DIP-6043N |
ELMi | P10747 |
IntActi | P10747, 10 interactors |
MINTi | P10747 |
STRINGi | 9606.ENSP00000324890 |
Miscellaneous databases
RNActi | P10747, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10747 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10747 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 137 | Ig-like V-typeAdd BLAST | 110 |
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SAVP, Eukaryota |
GeneTreei | ENSGT00530000063873 |
HOGENOMi | CLU_154699_0_0_1 |
InParanoidi | P10747 |
OMAi | HQLQFHS |
OrthoDBi | 1222373at2759 |
PhylomeDBi | P10747 |
TreeFami | TF335679 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00509 |
InterProi | View protein in InterPro IPR008093, CD28 IPR040216, CTLA4/CD28 IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013106, Ig_V-set |
PANTHERi | PTHR11494, PTHR11494, 1 hit |
Pfami | View protein in Pfam PF15910, V-set_2, 1 hit |
PRINTSi | PR01717, CD28ANTIGEN |
SMARTi | View protein in SMART SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
s (7)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P10747-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLRLLLALNL FPSIQVTGNK ILVKQSPMLV AYDNAVNLSC KYSYNLFSRE
60 70 80 90 100
FRASLHKGLD SAVEVCVVYG NYSQQLQVYS KTGFNCDGKL GNESVTFYLQ
110 120 130 140 150
NLYVNQTDIY FCKIEVMYPP PYLDNEKSNG TIIHVKGKHL CPSPLFPGPS
160 170 180 190 200
KPFWVLVVVG GVLACYSLLV TVAFIIFWVR SKRSRLLHSD YMNMTPRRPG
210 220
PTRKHYQPYA PPRDFAAYRS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047701 | 1 – 17 | MLRLL…SIQVT → MPCGLSALIMCPKGMVAVVV AVDDGDSQALA in isoform 7. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_002494 | 19 – 137 | Missing in isoform 2. 1 PublicationAdd BLAST | 119 | |
Alternative sequenceiVSP_002496 | 40 – 137 | CKYSY…IHVKG → W in isoform 4. 1 PublicationAdd BLAST | 98 | |
Alternative sequenceiVSP_002495 | 40 – 124 | CKYSY…PPYLD → Y in isoform 3, isoform 5 and isoform 6. 2 PublicationsAdd BLAST | 85 | |
Alternative sequenceiVSP_002497 | 138 – 139 | KH → EE in isoform 5. 1 Publication | 2 | |
Alternative sequenceiVSP_002498 | 140 – 220 | Missing in isoform 5. 1 PublicationAdd BLAST | 81 | |
Alternative sequenceiVSP_002499 | 152 – 207 | Missing in isoform 6. 1 PublicationAdd BLAST | 56 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000324106; ENSP00000324890; ENSG00000178562 [P10747-1] ENST00000374481; ENSP00000363605; ENSG00000178562 [P10747-2] ENST00000458610; ENSP00000393648; ENSG00000178562 [P10747-7] |
GeneIDi | 940 |
KEGGi | hsa:940 |
UCSCi | uc002vah.6, human [P10747-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia CD28 entry |
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YJD | X-ray | 2.70 | C | 17-152 | [»] | |
3WA4 | X-ray | 1.35 | B | 189-196 | [»] | |
5AUL | X-ray | 1.10 | B | 189-196 | [»] | |
5GJH | X-ray | 1.20 | B/D | 189-196 | [»] | |
5GJI | X-ray | 0.90 | B | 189-196 | [»] | |
6O8D | X-ray | 3.55 | C | 19-136 | [»] | |
SMRi | P10747 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107378, 20 interactors |
CORUMi | P10747 |
DIPi | DIP-6043N |
ELMi | P10747 |
IntActi | P10747, 10 interactors |
MINTi | P10747 |
STRINGi | 9606.ENSP00000324890 |
Chemistry databases
ChEMBLi | CHEMBL5191 |
GuidetoPHARMACOLOGYi | 2863 |
PTM databases
GlyGeni | P10747, 5 sites |
iPTMneti | P10747 |
PhosphoSitePlusi | P10747 |
Polymorphism and mutation databases
BioMutai | CD28 |
DMDMi | 115973 |
Proteomic databases
MassIVEi | P10747 |
MaxQBi | P10747 |
PaxDbi | P10747 |
PeptideAtlasi | P10747 |
PRIDEi | P10747 |
ProteomicsDBi | 52645 [P10747-1] 52646 [P10747-2] 52647 [P10747-3] 52648 [P10747-4] 52649 [P10747-5] 52650 [P10747-6] 68570 |
Protocols and materials databases
ABCDi | P10747, 16 sequenced antibodies |
Antibodypediai | 19958, 2227 antibodies |
Genome annotation databases
Ensembli | ENST00000324106; ENSP00000324890; ENSG00000178562 [P10747-1] ENST00000374481; ENSP00000363605; ENSG00000178562 [P10747-2] ENST00000458610; ENSP00000393648; ENSG00000178562 [P10747-7] |
GeneIDi | 940 |
KEGGi | hsa:940 |
UCSCi | uc002vah.6, human [P10747-1] |
Organism-specific databases
CTDi | 940 |
DisGeNETi | 940 |
EuPathDBi | HostDB:ENSG00000178562.17 |
GeneCardsi | CD28 |
HGNCi | HGNC:1653, CD28 |
HPAi | ENSG00000178562, Tissue enhanced (blood, lymphoid tissue) |
MalaCardsi | CD28 |
MIMi | 186760, gene |
neXtProti | NX_P10747 |
OpenTargetsi | ENSG00000178562 |
Orphaneti | 2584, Classic mycosis fungoides 3162, Sezary syndrome |
PharmGKBi | PA26207 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SAVP, Eukaryota |
GeneTreei | ENSGT00530000063873 |
HOGENOMi | CLU_154699_0_0_1 |
InParanoidi | P10747 |
OMAi | HQLQFHS |
OrthoDBi | 1222373at2759 |
PhylomeDBi | P10747 |
TreeFami | TF335679 |
Enzyme and pathway databases
PathwayCommonsi | P10747 |
Reactomei | R-HSA-1257604, PIP3 activates AKT signaling R-HSA-164939, Nef mediated downregulation of CD28 cell surface expression R-HSA-2219530, Constitutive Signaling by Aberrant PI3K in Cancer R-HSA-389356, CD28 co-stimulation R-HSA-389357, CD28 dependent PI3K/Akt signaling R-HSA-389359, CD28 dependent Vav1 pathway R-HSA-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling |
SignaLinki | P10747 |
SIGNORi | P10747 |
Miscellaneous databases
BioGRID-ORCSi | 940, 4 hits in 846 CRISPR screens |
ChiTaRSi | CD28, human |
EvolutionaryTracei | P10747 |
GeneWikii | CD28 |
GenomeRNAii | 940 |
Pharosi | P10747, Tbio |
PROi | PR:P10747 |
RNActi | P10747, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178562, Expressed in leukocyte and 124 other tissues |
Genevisiblei | P10747, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00509 |
InterProi | View protein in InterPro IPR008093, CD28 IPR040216, CTLA4/CD28 IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013106, Ig_V-set |
PANTHERi | PTHR11494, PTHR11494, 1 hit |
Pfami | View protein in Pfam PF15910, V-set_2, 1 hit |
PRINTSi | PR01717, CD28ANTIGEN |
SMARTi | View protein in SMART SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CD28_HUMAN | |
Accessioni | P10747Primary (citable) accession number: P10747 Secondary accession number(s): A8KAC1 Q9BYV0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 207 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries