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Protein

Transcription elongation factor A protein 1

Gene

Tcea1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

  • erythrocyte differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • transcription, DNA-templated Source: InterPro

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Transcription elongation factor TFIIS.o
Gene namesi
Name:Tcea1
Synonyms:Tceat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1196624 Tcea1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214471 – 301Transcription elongation factor A protein 1Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57PhosphoserineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei97PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP10711
PaxDbiP10711
PeptideAtlasiP10711
PRIDEiP10711

PTM databases

iPTMnetiP10711
PhosphoSitePlusiP10711

Expressioni

Gene expression databases

BgeeiENSMUSG00000033813 Expressed in 300 organ(s), highest expression level in spleen
CleanExiMM_TCEA1
ExpressionAtlasiP10711 baseline and differential
GenevisibleiP10711 MM

Interactioni

Subunit structurei

Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription by recruiting their promoters (By similarity). Interacts with EAF2.By similarity1 Publication

Protein-protein interaction databases

BioGridi203995, 1 interactor
IntActiP10711, 1 interactor
STRINGi10090.ENSMUSP00000129157

Structurei

3D structure databases

ProteinModelPortaliP10711
SMRiP10711
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 80TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78
Domaini140 – 256TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00390000017794
HOGENOMiHOG000195015
HOVERGENiHBG055022
InParanoidiP10711
KOiK03145
OMAiKKAQVPM
PhylomeDBiP10711

Family and domain databases

Gene3Di1.10.472.30, 1 hit
1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035100 TF_IIS-typ
IPR003617 TFIIS/CRSP70_N_sub
IPR035441 TFIIS/LEDGF_dom_sf
IPR003618 TFIIS_cen_dom
IPR036575 TFIIS_cen_dom_sf
IPR017923 TFIIS_N
IPR006289 TFSII
IPR001222 Znf_TFIIS
PfamiView protein in Pfam
PF08711 Med26, 1 hit
PF01096 TFIIS_C, 1 hit
PF07500 TFIIS_M, 1 hit
PIRSFiPIRSF006704 TF_IIS, 1 hit
SMARTiView protein in SMART
SM00510 TFS2M, 1 hit
SM00509 TFS2N, 1 hit
SM00440 ZnF_C2C2, 1 hit
SUPFAMiSSF46942 SSF46942, 1 hit
SSF47676 SSF47676, 1 hit
TIGRFAMsiTIGR01385 TFSII, 1 hit
PROSITEiView protein in PROSITE
PS51321 TFIIS_CENTRAL, 1 hit
PS51319 TFIIS_N, 1 hit
PS00466 ZF_TFIIS_1, 1 hit
PS51133 ZF_TFIIS_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 2 (identifier: P10711-1) [UniParc]FASTAAdd to basket
Also known as: PSII-3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NALRKQSTDE EVTSLAKSLI KSWKKLLDGP STDKDPEEKK KEPAISSQNS
110 120 130 140 150
PEAREESSSS SNVSSRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYVA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,880
Last modified:February 12, 2003 - v2
Checksum:i14B5105F8D77DA87
GO
Isoform 1 (identifier: P10711-2) [UniParc]FASTAAdd to basket
Also known as: PSII-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Show »
Length:266
Mass (Da):29,930
Checksum:iEFFB2585B81B3F54
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PYD5E9PYD5_MOUSE
Transcription elongation factor A p...
Tcea1
312Annotation score:

Sequence cautioni

The sequence BAA00768 differs from that shown. Chimeric cDNA.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0064101 – 35Missing in isoform 1. 2 PublicationsAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18209 mRNA Translation: AAA40418.1
M18210 mRNA Translation: AAA40419.1
D00925 mRNA Translation: BAA00768.1 Sequence problems.
BC006022 mRNA Translation: AAH06022.1
BC061490 mRNA Translation: AAH61490.1
BC083127 mRNA Translation: AAH83127.1
CCDSiCCDS35505.1 [P10711-1]
PIRiA29950
RefSeqiNP_001153222.1, NM_001159750.1
NP_001153223.1, NM_001159751.1
NP_035671.1, NM_011541.4 [P10711-1]
UniGeneiMm.207263

Genome annotation databases

EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813 [P10711-1]
GeneIDi21399
KEGGimmu:21399
UCSCiuc007afi.2 mouse [P10711-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18209 mRNA Translation: AAA40418.1
M18210 mRNA Translation: AAA40419.1
D00925 mRNA Translation: BAA00768.1 Sequence problems.
BC006022 mRNA Translation: AAH06022.1
BC061490 mRNA Translation: AAH61490.1
BC083127 mRNA Translation: AAH83127.1
CCDSiCCDS35505.1 [P10711-1]
PIRiA29950
RefSeqiNP_001153222.1, NM_001159750.1
NP_001153223.1, NM_001159751.1
NP_035671.1, NM_011541.4 [P10711-1]
UniGeneiMm.207263

3D structure databases

ProteinModelPortaliP10711
SMRiP10711
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203995, 1 interactor
IntActiP10711, 1 interactor
STRINGi10090.ENSMUSP00000129157

PTM databases

iPTMnetiP10711
PhosphoSitePlusiP10711

Proteomic databases

EPDiP10711
PaxDbiP10711
PeptideAtlasiP10711
PRIDEiP10711

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081551; ENSMUSP00000080266; ENSMUSG00000033813 [P10711-1]
GeneIDi21399
KEGGimmu:21399
UCSCiuc007afi.2 mouse [P10711-1]

Organism-specific databases

CTDi6917
MGIiMGI:1196624 Tcea1

Phylogenomic databases

eggNOGiKOG1105 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00390000017794
HOGENOMiHOG000195015
HOVERGENiHBG055022
InParanoidiP10711
KOiK03145
OMAiKKAQVPM
PhylomeDBiP10711

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiTcea1 mouse
PROiPR:P10711
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033813 Expressed in 300 organ(s), highest expression level in spleen
CleanExiMM_TCEA1
ExpressionAtlasiP10711 baseline and differential
GenevisibleiP10711 MM

Family and domain databases

Gene3Di1.10.472.30, 1 hit
1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035100 TF_IIS-typ
IPR003617 TFIIS/CRSP70_N_sub
IPR035441 TFIIS/LEDGF_dom_sf
IPR003618 TFIIS_cen_dom
IPR036575 TFIIS_cen_dom_sf
IPR017923 TFIIS_N
IPR006289 TFSII
IPR001222 Znf_TFIIS
PfamiView protein in Pfam
PF08711 Med26, 1 hit
PF01096 TFIIS_C, 1 hit
PF07500 TFIIS_M, 1 hit
PIRSFiPIRSF006704 TF_IIS, 1 hit
SMARTiView protein in SMART
SM00510 TFS2M, 1 hit
SM00509 TFS2N, 1 hit
SM00440 ZnF_C2C2, 1 hit
SUPFAMiSSF46942 SSF46942, 1 hit
SSF47676 SSF47676, 1 hit
TIGRFAMsiTIGR01385 TFSII, 1 hit
PROSITEiView protein in PROSITE
PS51321 TFIIS_CENTRAL, 1 hit
PS51319 TFIIS_N, 1 hit
PS00466 ZF_TFIIS_1, 1 hit
PS51133 ZF_TFIIS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTCEA1_MOUSE
AccessioniPrimary (citable) accession number: P10711
Secondary accession number(s): P10712, P23713
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 12, 2003
Last modified: November 7, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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