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UniProtKB - P10696 (PPBN_HUMAN)
Protein
Alkaline phosphatase, germ cell type
Gene
ALPG
Organism
Homo sapiens (Human)
Status
Functioni
Alkaline phosphatase that can hydrolyze various phosphate compounds.
1 PublicationMiscellaneous
In most mammals there are four different isozymes: placental (ALPP), germ cell (ALPG), intestinal (ALPI) and tissue non-specific (liver/bone/kidney) (ALPL/TNAP).Curated
Catalytic activityi
- EC:3.1.3.1PROSITE-ProRule annotation1 Publication
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
- Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity
- Ca2+By similarity
Activity regulationi
Inhibited by L-leucine, EDTA and heat.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 61 | MagnesiumBy similarity | 1 | |
Metal bindingi | 61 | Zinc 1By similarity | 1 | |
Active sitei | 111 | Phosphoserine intermediatePROSITE-ProRule annotation | 1 | |
Metal bindingi | 111 | Zinc 1By similarity | 1 | |
Metal bindingi | 174 | MagnesiumBy similarity | 1 | |
Metal bindingi | 235 | CalciumBy similarity | 1 | |
Metal bindingi | 288 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 289 | CalciumBy similarity | 1 | |
Metal bindingi | 304 | CalciumBy similarity | 1 | |
Metal bindingi | 330 | MagnesiumBy similarity | 1 | |
Metal bindingi | 335 | Zinc 2By similarity | 1 | |
Metal bindingi | 339 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 376 | Zinc 1By similarity | 1 | |
Metal bindingi | 377 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 451 | Zinc 2; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- alkaline phosphatase activity Source: HGNC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dephosphorylation Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P10696 |
Reactomei | R-HSA-163125, Post-translational modification: synthesis of GPI-anchored proteins |
SignaLinki | P10696 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline phosphatase, germ cell typeCurated (EC:3.1.3.1PROSITE-ProRule annotation1 Publication)Alternative name(s): ALP-1 Alkaline phosphatase Nagao isozyme Alkaline phosphatase, placental-like Germ cell alkaline phosphatase1 Publication Short name: GCAP1 Publication Placental alkaline phosphatase-like Short name: PLAP-like |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:441, ALPG |
MIMi | 171810, gene |
neXtProti | NX_P10696 |
VEuPathDBi | HostDB:ENSG00000163286 |
Subcellular locationi
Plasma membrane
Extracellular region or secreted
- extracellular region Source: Reactome
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 251 |
OpenTargetsi | ENSG00000163286 |
PharmGKBi | PA24731 |
Miscellaneous databases
Pharosi | P10696, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3402 |
DrugBanki | DB01143, Amifostine DB11091, Hydrogen peroxide DB00848, Levamisole DB09498, Strontium chloride Sr-89 DB09322, Zinc sulfate |
Genetic variation databases
BioMutai | ALPPL2 |
DMDMi | 145559564 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000024033 | 20 – 503 | Alkaline phosphatase, germ cell typeAdd BLAST | 484 | |
PropeptideiPRO_0000024034 | 504 – 532 | Removed in mature formSequence analysisAdd BLAST | 29 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 140 ↔ 202 | By similarity | ||
Glycosylationi | 141 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 268 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 486 ↔ 493 | By similarity | ||
Lipidationi | 503 | GPI-anchor amidated aspartateSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
EPDi | P10696 |
jPOSTi | P10696 |
MassIVEi | P10696 |
MaxQBi | P10696 |
PaxDbi | P10696 |
PeptideAtlasi | P10696 |
PRIDEi | P10696 |
ProteomicsDBi | 52638 |
PTM databases
DEPODi | ALPG |
GlyGeni | P10696, 2 sites |
iPTMneti | P10696 |
PhosphoSitePlusi | P10696 |
Expressioni
Tissue specificityi
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
Gene expression databases
Bgeei | ENSG00000163286, Expressed in placenta and 64 other tissues |
Genevisiblei | P10696, HS |
Organism-specific databases
HPAi | ENSG00000163286, Not detected |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 106752, 30 interactors |
IntActi | P10696, 12 interactors |
STRINGi | 9606.ENSP00000295453 |
Chemistry databases
BindingDBi | P10696 |
Miscellaneous databases
RNActi | P10696, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 422 – 443 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the alkaline phosphatase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
InParanoidi | P10696 |
OMAi | FEYSHME |
OrthoDBi | 454880at2759 |
PhylomeDBi | P10696 |
TreeFami | TF323513 |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10696-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT
60 70 80 90 100
AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK
110 120 130 140 150
TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK
260 270 280 290 300
NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD
310 320 330 340 350
STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET
360 370 380 390 400
IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK
410 420 430 440 450
ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET
460 470 480 490 500
HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG
510 520 530
TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP
Sequence cautioni
The sequence CAA30232 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | I → M in AAA98616 (PubMed:2834730).Curated | 1 | |
Sequence conflicti | 152 | M → V in AAA98616 (PubMed:2834730).Curated | 1 | |
Sequence conflicti | 152 | M → V in CAA39425 (PubMed:2162249).Curated | 1 | |
Sequence conflicti | 178 | A → T in AAA98616 (PubMed:2834730).Curated | 1 | |
Sequence conflicti | 178 | A → T in AAA51700 (PubMed:2745460).Curated | 1 | |
Sequence conflicti | 178 | A → T in CAA39425 (PubMed:2162249).Curated | 1 | |
Sequence conflicti | 260 | H → R in AAH14139 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 380 | V → L in AAA51700 (PubMed:2745460).Curated | 1 | |
Sequence conflicti | 498 | R → P in AAA98616 (PubMed:2834730).Curated | 1 | |
Sequence conflicti | 498 | R → P in CAA39425 (PubMed:2162249).Curated | 1 | |
Sequence conflicti | 498 | R → S in CAA37374 (PubMed:2297757).Curated | 1 | |
Sequence conflicti | 531 | A → T in CAA37374 (PubMed:2297757).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027553 | 273 | L → M2 PublicationsCorresponds to variant dbSNP:rs17416141Ensembl. | 1 | |
Natural variantiVAR_027554 | 316 | L → R5 PublicationsCorresponds to variant dbSNP:rs183793479Ensembl. | 1 | |
Natural variantiVAR_027555 | 527 | G → E. Corresponds to variant dbSNP:rs1048999Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03252 Genomic DNA Translation: AAA98616.1 J04948 mRNA Translation: AAA51700.1 X53279 mRNA Translation: CAA37374.1 X55958 mRNA Translation: CAA39425.1 AK293017 mRNA Translation: BAF85706.1 AC068134 Genomic DNA Translation: AAY24088.1 CH471063 Genomic DNA Translation: EAW70994.1 BC014139 mRNA Translation: AAH14139.1 M19160 Genomic DNA Translation: AAA51707.1 X07247 Genomic DNA Translation: CAA30232.1 Sequence problems. |
CCDSi | CCDS2491.1 |
PIRi | S12076 |
RefSeqi | NP_112603.2, NM_031313.2 |
Genome annotation databases
Ensembli | ENST00000295453; ENSP00000295453; ENSG00000163286 |
GeneIDi | 251 |
KEGGi | hsa:251 |
MANE-Selecti | ENST00000295453.8; ENSP00000295453.3; NM_031313.3; NP_112603.2 |
UCSCi | uc002vss.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03252 Genomic DNA Translation: AAA98616.1 J04948 mRNA Translation: AAA51700.1 X53279 mRNA Translation: CAA37374.1 X55958 mRNA Translation: CAA39425.1 AK293017 mRNA Translation: BAF85706.1 AC068134 Genomic DNA Translation: AAY24088.1 CH471063 Genomic DNA Translation: EAW70994.1 BC014139 mRNA Translation: AAH14139.1 M19160 Genomic DNA Translation: AAA51707.1 X07247 Genomic DNA Translation: CAA30232.1 Sequence problems. |
CCDSi | CCDS2491.1 |
PIRi | S12076 |
RefSeqi | NP_112603.2, NM_031313.2 |
3D structure databases
SMRi | P10696 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106752, 30 interactors |
IntActi | P10696, 12 interactors |
STRINGi | 9606.ENSP00000295453 |
Chemistry databases
BindingDBi | P10696 |
ChEMBLi | CHEMBL3402 |
DrugBanki | DB01143, Amifostine DB11091, Hydrogen peroxide DB00848, Levamisole DB09498, Strontium chloride Sr-89 DB09322, Zinc sulfate |
PTM databases
DEPODi | ALPG |
GlyGeni | P10696, 2 sites |
iPTMneti | P10696 |
PhosphoSitePlusi | P10696 |
Genetic variation databases
BioMutai | ALPPL2 |
DMDMi | 145559564 |
Proteomic databases
EPDi | P10696 |
jPOSTi | P10696 |
MassIVEi | P10696 |
MaxQBi | P10696 |
PaxDbi | P10696 |
PeptideAtlasi | P10696 |
PRIDEi | P10696 |
ProteomicsDBi | 52638 |
Protocols and materials databases
Antibodypediai | 20216, 221 antibodies from 30 providers |
DNASUi | 251 |
Genome annotation databases
Ensembli | ENST00000295453; ENSP00000295453; ENSG00000163286 |
GeneIDi | 251 |
KEGGi | hsa:251 |
MANE-Selecti | ENST00000295453.8; ENSP00000295453.3; NM_031313.3; NP_112603.2 |
UCSCi | uc002vss.5, human |
Organism-specific databases
CTDi | 251 |
DisGeNETi | 251 |
GeneCardsi | ALPG |
HGNCi | HGNC:441, ALPG |
HPAi | ENSG00000163286, Not detected |
MIMi | 171810, gene |
neXtProti | NX_P10696 |
OpenTargetsi | ENSG00000163286 |
PharmGKBi | PA24731 |
VEuPathDBi | HostDB:ENSG00000163286 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
InParanoidi | P10696 |
OMAi | FEYSHME |
OrthoDBi | 454880at2759 |
PhylomeDBi | P10696 |
TreeFami | TF323513 |
Enzyme and pathway databases
PathwayCommonsi | P10696 |
Reactomei | R-HSA-163125, Post-translational modification: synthesis of GPI-anchored proteins |
SignaLinki | P10696 |
Miscellaneous databases
BioGRID-ORCSi | 251, 3 hits in 965 CRISPR screens |
GenomeRNAii | 251 |
Pharosi | P10696, Tchem |
PROi | PR:P10696 |
RNActi | P10696, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163286, Expressed in placenta and 64 other tissues |
Genevisiblei | P10696, HS |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPBN_HUMAN | |
Accessioni | P10696Primary (citable) accession number: P10696 Secondary accession number(s): A8KAF2 Q96CM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | March 6, 2007 | |
Last modified: | February 23, 2022 | |
This is version 201 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families