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Protein

Alkaline phosphatase, placental-like

Gene

ALPPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediate1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi451Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: HGNC
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental-like (EC:3.1.3.1)
Alternative name(s):
ALP-1
Alkaline phosphatase Nagao isozyme
Germ cell alkaline phosphatase
Short name:
GCAP
Placental alkaline phosphatase-like
Short name:
PLAP-like
Gene namesi
Name:ALPPL2
Synonyms:ALPPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163286.7
HGNCiHGNC:441 ALPPL2
MIMi171810 gene
neXtProtiNX_P10696

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi251
OpenTargetsiENSG00000163286
PharmGKBiPA24731

Chemistry databases

ChEMBLiCHEMBL3402
DrugBankiDB01143 Amifostine
DB00848 Levamisole
DB09322 Zinc sulfate

Polymorphism and mutation databases

BioMutaiALPPL2
DMDMi145559564

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002403320 – 503Alkaline phosphatase, placental-likeAdd BLAST484
PropeptideiPRO_0000024034504 – 532Removed in mature formBy similarityAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi486 ↔ 493By similarity
Lipidationi503GPI-anchor amidated aspartateBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP10696
MaxQBiP10696
PaxDbiP10696
PeptideAtlasiP10696
PRIDEiP10696
ProteomicsDBi52638

PTM databases

DEPODiP10696
iPTMnetiP10696
PhosphoSitePlusiP10696

Expressioni

Tissue specificityi

Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Gene expression databases

BgeeiENSG00000163286
CleanExiHS_ALPPL2
GenevisibleiP10696 HS

Organism-specific databases

HPAiCAB020698
HPA038764
HPA038765
HPA051699

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi106752, 9 interactors
IntActiP10696, 3 interactors
STRINGi9606.ENSP00000295453

Chemistry databases

BindingDBiP10696

Structurei

3D structure databases

ProteinModelPortaliP10696
SMRiP10696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP10696
KOiK01077
OMAiMKNKDFM
OrthoDBiEOG091G067H
PhylomeDBiP10696
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT
60 70 80 90 100
AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK
110 120 130 140 150
TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK
260 270 280 290 300
NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD
310 320 330 340 350
STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET
360 370 380 390 400
IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK
410 420 430 440 450
ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET
460 470 480 490 500
HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG
510 520 530
TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP
Length:532
Mass (Da):57,377
Last modified:March 6, 2007 - v4
Checksum:i25EB56C901B61505
GO

Sequence cautioni

The sequence CAA30232 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57I → M in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti152M → V in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti152M → V in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti178A → T in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti178A → T in AAA51700 (PubMed:2745460).Curated1
Sequence conflicti178A → T in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti260H → R in AAH14139 (PubMed:15489334).Curated1
Sequence conflicti380V → L in AAA51700 (PubMed:2745460).Curated1
Sequence conflicti498R → P in AAA98616 (PubMed:2834730).Curated1
Sequence conflicti498R → P in CAA39425 (PubMed:2162249).Curated1
Sequence conflicti498R → S in CAA37374 (PubMed:2297757).Curated1
Sequence conflicti531A → T in CAA37374 (PubMed:2297757).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027553273L → M2 PublicationsCorresponds to variant dbSNP:rs17416141Ensembl.1
Natural variantiVAR_027554316L → R5 PublicationsCorresponds to variant dbSNP:rs183793479Ensembl.1
Natural variantiVAR_027555527G → E. Corresponds to variant dbSNP:rs1048999Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03252 Genomic DNA Translation: AAA98616.1
J04948 mRNA Translation: AAA51700.1
X53279 mRNA Translation: CAA37374.1
X55958 mRNA Translation: CAA39425.1
AK293017 mRNA Translation: BAF85706.1
AC068134 Genomic DNA Translation: AAY24088.1
CH471063 Genomic DNA Translation: EAW70994.1
BC014139 mRNA Translation: AAH14139.1
M19160 Genomic DNA Translation: AAA51707.1
X07247 Genomic DNA Translation: CAA30232.1 Sequence problems.
CCDSiCCDS2491.1
PIRiS12076
RefSeqiNP_112603.2, NM_031313.2
UniGeneiHs.333509

Genome annotation databases

EnsembliENST00000295453; ENSP00000295453; ENSG00000163286
GeneIDi251
KEGGihsa:251
UCSCiuc002vss.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPPBN_HUMAN
AccessioniPrimary (citable) accession number: P10696
Secondary accession number(s): A8KAF2
, Q16727, Q53S81, Q96CM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: March 6, 2007
Last modified: July 18, 2018
This is version 179 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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