Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thioredoxin

Gene

Txn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity).By similarity
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei26Deprotonates C-terminal active site CysBy similarity1
Active sitei32NucleophileBy similarity1
Sitei33Contributes to redox potential valueBy similarity1
Sitei34Contributes to redox potential valueBy similarity1
Active sitei35NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processElectron transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-499943 Interconversion of nucleotide di- and triphosphates
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-5676934 Protein repair
R-MMU-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin
Short name:
Trx
Alternative name(s):
ATL-derived factor
Short name:
ADF
Gene namesi
Name:Txn
Synonyms:Txn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:98874 Txn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001200071 – 105ThioredoxinAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei8N6-succinyllysineCombined sources1
Disulfide bondi32 ↔ 35Redox-activePROSITE-ProRule annotation
Modified residuei39N6-acetyllysineCombined sources1
Modified residuei62S-nitrosocysteineBy similarity1
Modified residuei69S-nitrosocysteineBy similarity1
Modified residuei73S-nitrosocysteine; alternateBy similarity1
Disulfide bondi73Interchain; alternateBy similarity
Modified residuei94N6-acetyllysine; alternateCombined sources1
Modified residuei94N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins (By similarity).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, S-nitrosylation

Proteomic databases

EPDiP10639
MaxQBiP10639
PaxDbiP10639
PeptideAtlasiP10639
PRIDEiP10639
TopDownProteomicsiP10639

2D gel databases

SWISS-2DPAGEiP10639
UCD-2DPAGEiP10639

PTM databases

iPTMnetiP10639
PhosphoSitePlusiP10639
SwissPalmiP10639

Expressioni

Gene expression databases

BgeeiENSMUSG00000028367 Expressed in 27 organ(s), highest expression level in colon
CleanExiMM_TXN1
GenevisibleiP10639 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204389, 5 interactors
IntActiP10639, 4 interactors
MINTiP10639
STRINGi10090.ENSMUSP00000030051

Structurei

3D structure databases

ProteinModelPortaliP10639
SMRiP10639
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 105ThioredoxinPROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Belongs to the thioredoxin family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0907 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00530000063008
HOGENOMiHOG000292977
HOVERGENiHBG009243
InParanoidiP10639
KOiK03671
OMAiDANQEFA
OrthoDBiEOG091G01FG
PhylomeDBiP10639
TreeFamiTF318932

Family and domain databases

InterProiView protein in InterPro
IPR005746 Thioredoxin
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PANTHERiPTHR10438 PTHR10438, 1 hit
PfamiView protein in Pfam
PF00085 Thioredoxin, 1 hit
PIRSFiPIRSF000077 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P10639-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS
60 70 80 90 100
NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAS

ITEYA
Length:105
Mass (Da):11,675
Last modified:January 23, 2007 - v3
Checksum:i3A2B925935F952DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100S → C in BAB25256 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77585 mRNA Translation: CAA54688.1
D21859 Genomic DNA Translation: BAA04881.1
AK007537 mRNA Translation: BAB25096.1
AK007790 mRNA Translation: BAB25256.1
BC010756 mRNA Translation: AAH10756.1
BC094415 mRNA Translation: AAH94415.1
CCDSiCCDS18207.1
PIRiJC4068 S04107
RefSeqiNP_035790.1, NM_011660.3
UniGeneiMm.260618

Genome annotation databases

EnsembliENSMUST00000030051; ENSMUSP00000030051; ENSMUSG00000028367
GeneIDi22166
KEGGimmu:22166
UCSCiuc008syp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTHIO_MOUSE
AccessioniPrimary (citable) accession number: P10639
Secondary accession number(s): Q52KC4, Q9D8R0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 172 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again