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Protein

Eukaryotic initiation factor 4A-II

Gene

Eif4a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-II (EC:3.6.4.13)
Short name:
eIF-4A-II
Short name:
eIF4A-II
Alternative name(s):
ATP-dependent RNA helicase eIF4A-2
Gene namesi
Name:Eif4a2
Synonyms:Ddx2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:106906 Eif4a2

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3751650

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549401 – 407Eukaryotic initiation factor 4A-IIAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP10630
PaxDbiP10630
PeptideAtlasiP10630
PRIDEiP10630

PTM databases

iPTMnetiP10630
PhosphoSitePlusiP10630

Expressioni

Gene expression databases

BgeeiENSMUSG00000022884 Expressed in 287 organ(s), highest expression level in cerebral cortex
CleanExiMM_EIF4A2
ExpressionAtlasiP10630 baseline and differential
GenevisibleiP10630 MM

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3. Interacts with EIF4E. May interact with NOM1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199419, 7 interactors
IntActiP10630, 6 interactors
MINTiP10630
STRINGi10090.ENSMUSP00000023599

Structurei

3D structure databases

ProteinModelPortaliP10630
SMRiP10630
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 235Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini246 – 407Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi33 – 61Q motifAdd BLAST29
Motifi183 – 186DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0327 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00530000062880
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiP10630
KOiK03257
OMAiRNPIRIL
OrthoDBiEOG091G07OI
PhylomeDBiP10630
TreeFamiTF101524

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR037583 EIF4A
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PANTHERiPTHR24031:SF226 PTHR24031:SF226, 1 hit
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P10630-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA
60 70 80 90 100
YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK
110 120 130 140 150
ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA
160 170 180 190 200
EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI
210 220 230 240 250
FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF
260 270 280 290 300
YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV
310 320 330 340 350
SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL
360 370 380 390 400
PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP

MNVADLI
Length:407
Mass (Da):46,402
Last modified:November 1, 1997 - v2
Checksum:iFAA7D3BA9D8C6DA0
GO
Isoform 2 (identifier: P10630-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-9: N → NS

Show »
Length:408
Mass (Da):46,489
Checksum:i0F47C0C4EEF00DD3
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BTU6Q8BTU6_MOUSE
Eukaryotic initiation factor 4A-II
Eif4a2
362Annotation score:
E9Q561E9Q561_MOUSE
Eukaryotic initiation factor 4A-II
Eif4a2
312Annotation score:
D6RJ11D6RJ11_MOUSE
Eukaryotic initiation factor 4A-II
Eif4a2
97Annotation score:
D6RJ60D6RJ60_MOUSE
Eukaryotic initiation factor 4A-II
Eif4a2 mCG_128248
96Annotation score:
A0A338P6X5A0A338P6X5_MOUSE
Eukaryotic initiation factor 4A-II
Eif4a2
322Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200 – 201IF → RV in CAA31025 (PubMed:3046931).Curated2
Sequence conflicti294 – 297HARD → QAIY in CAA31025 (PubMed:3046931).Curated4
Sequence conflicti387D → H in CAA31025 (PubMed:3046931).Curated1
Sequence conflicti403V → G in CAA31025 (PubMed:3046931).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096309N → NS in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12507 mRNA Translation: CAA31025.1
X14422 Genomic DNA Translation: CAA32585.1
X56953 Genomic DNA Translation: CAA40269.1
X56953 Genomic DNA Translation: CAA40268.1
AK076509 mRNA Translation: BAC36372.1
U64706 Genomic DNA Translation: AAC53180.1
CCDSiCCDS28074.1 [P10630-1]
CCDS84218.1 [P10630-2]
PIRiS00985
RefSeqiNP_001116509.1, NM_001123037.2
NP_001116510.1, NM_001123038.2
NP_001334108.1, NM_001347179.1 [P10630-2]
NP_038534.2, NM_013506.3 [P10630-1]
UniGeneiMm.260084

Genome annotation databases

EnsembliENSMUST00000023599; ENSMUSP00000023599; ENSMUSG00000022884 [P10630-1]
ENSMUST00000115341; ENSMUSP00000110998; ENSMUSG00000022884 [P10630-2]
GeneIDi13682
KEGGimmu:13682
UCSCiuc007ytd.3 mouse [P10630-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12507 mRNA Translation: CAA31025.1
X14422 Genomic DNA Translation: CAA32585.1
X56953 Genomic DNA Translation: CAA40269.1
X56953 Genomic DNA Translation: CAA40268.1
AK076509 mRNA Translation: BAC36372.1
U64706 Genomic DNA Translation: AAC53180.1
CCDSiCCDS28074.1 [P10630-1]
CCDS84218.1 [P10630-2]
PIRiS00985
RefSeqiNP_001116509.1, NM_001123037.2
NP_001116510.1, NM_001123038.2
NP_001334108.1, NM_001347179.1 [P10630-2]
NP_038534.2, NM_013506.3 [P10630-1]
UniGeneiMm.260084

3D structure databases

ProteinModelPortaliP10630
SMRiP10630
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199419, 7 interactors
IntActiP10630, 6 interactors
MINTiP10630
STRINGi10090.ENSMUSP00000023599

Chemistry databases

ChEMBLiCHEMBL3751650

PTM databases

iPTMnetiP10630
PhosphoSitePlusiP10630

Proteomic databases

EPDiP10630
PaxDbiP10630
PeptideAtlasiP10630
PRIDEiP10630

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023599; ENSMUSP00000023599; ENSMUSG00000022884 [P10630-1]
ENSMUST00000115341; ENSMUSP00000110998; ENSMUSG00000022884 [P10630-2]
GeneIDi13682
KEGGimmu:13682
UCSCiuc007ytd.3 mouse [P10630-1]

Organism-specific databases

CTDi1974
MGIiMGI:106906 Eif4a2

Phylogenomic databases

eggNOGiKOG0327 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00530000062880
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiP10630
KOiK03257
OMAiRNPIRIL
OrthoDBiEOG091G07OI
PhylomeDBiP10630
TreeFamiTF101524

Miscellaneous databases

ChiTaRSiEif4b mouse
PROiPR:P10630
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022884 Expressed in 287 organ(s), highest expression level in cerebral cortex
CleanExiMM_EIF4A2
ExpressionAtlasiP10630 baseline and differential
GenevisibleiP10630 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR037583 EIF4A
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PANTHERiPTHR24031:SF226 PTHR24031:SF226, 1 hit
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF4A2_MOUSE
AccessioniPrimary (citable) accession number: P10630
Secondary accession number(s): Q61513, Q61514
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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