UniProtKB - P10622 (RLA3_YEAST)
60S acidic ribosomal protein P1-beta
RPP1B
Functioni
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
1 PublicationMiscellaneous
GO - Molecular functioni
- protein kinase activator activity Source: SGD
- ribonucleoprotein complex binding Source: GO_Central
- structural constituent of ribosome Source: SGD
GO - Biological processi
- cytoplasmic translation Source: SGD
- positive regulation of protein kinase activity Source: SGD
- translational elongation Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane R-SCE-72689, Formation of a pool of free 40S subunits R-SCE-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 60S acidic ribosomal protein P1-beta1 PublicationAlternative name(s): Ax L12eIIB L44' Large ribosomal subunit protein P1-B1 Publication Short name: P1B YP1beta |
Gene namesi | Ordered Locus Names:YDL130W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002288, RPP1B |
VEuPathDBi | FungiDB:YDL130W |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytosol
- cytosolic large ribosomal subunit Source: SGD
Vacuole
- fungal-type vacuole Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000157706 | 2 – 106 | 60S acidic ribosomal protein P1-betaAdd BLAST | 105 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 | |
Modified residuei | 96 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P10622 |
PaxDbi | P10622 |
PRIDEi | P10622 |
TopDownProteomicsi | P10622 |
PTM databases
iPTMneti | P10622 |
Interactioni
Subunit structurei
Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A (PubMed:16573688, PubMed:11431471).
1 Publication3 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 31933, 228 interactors |
DIPi | DIP-2055N |
IntActi | P10622, 29 interactors |
MINTi | P10622 |
STRINGi | 4932.YDL130W |
Miscellaneous databases
RNActi | P10622, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 106 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 84 – 106 | Acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1762, Eukaryota |
HOGENOMi | CLU_114656_1_0_1 |
InParanoidi | P10622 |
OMAi | DQVWADV |
Family and domain databases
Gene3Di | 1.10.10.1410, 1 hit |
HAMAPi | MF_01478, Ribosomal_L12_arch, 1 hit |
InterProi | View protein in InterPro IPR038716, P1/P2_N_sf IPR027534, Ribosomal_L12/P1/P2 |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSDSIISFAA FILADAGLEI TSDNLLTITK AAGANVDNVW ADVYAKALEG
60 70 80 90 100
KDLKEILSGF HNAGPVAGAG AASGAAAAGG DAAAEEEKEE EAAEESDDDM
GFGLFD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19238 Genomic DNA Translation: AAA34973.1 M26507 Genomic DNA Translation: AAA34734.1 Z74178 Genomic DNA Translation: CAA98698.1 BK006938 Genomic DNA Translation: DAA11730.1 |
PIRi | C28104, R8BY2B |
RefSeqi | NP_010153.1, NM_001180189.1 |
Genome annotation databases
EnsemblFungii | YDL130W_mRNA; YDL130W; YDL130W |
GeneIDi | 851427 |
KEGGi | sce:YDL130W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19238 Genomic DNA Translation: AAA34973.1 M26507 Genomic DNA Translation: AAA34734.1 Z74178 Genomic DNA Translation: CAA98698.1 BK006938 Genomic DNA Translation: DAA11730.1 |
PIRi | C28104, R8BY2B |
RefSeqi | NP_010153.1, NM_001180189.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31933, 228 interactors |
DIPi | DIP-2055N |
IntActi | P10622, 29 interactors |
MINTi | P10622 |
STRINGi | 4932.YDL130W |
PTM databases
iPTMneti | P10622 |
Proteomic databases
MaxQBi | P10622 |
PaxDbi | P10622 |
PRIDEi | P10622 |
TopDownProteomicsi | P10622 |
Genome annotation databases
EnsemblFungii | YDL130W_mRNA; YDL130W; YDL130W |
GeneIDi | 851427 |
KEGGi | sce:YDL130W |
Organism-specific databases
SGDi | S000002288, RPP1B |
VEuPathDBi | FungiDB:YDL130W |
Phylogenomic databases
eggNOGi | KOG1762, Eukaryota |
HOGENOMi | CLU_114656_1_0_1 |
InParanoidi | P10622 |
OMAi | DQVWADV |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane R-SCE-72689, Formation of a pool of free 40S subunits R-SCE-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
PROi | PR:P10622 |
RNActi | P10622, protein |
Family and domain databases
Gene3Di | 1.10.10.1410, 1 hit |
HAMAPi | MF_01478, Ribosomal_L12_arch, 1 hit |
InterProi | View protein in InterPro IPR038716, P1/P2_N_sf IPR027534, Ribosomal_L12/P1/P2 |
MobiDBi | Search... |
Entry informationi
Entry namei | RLA3_YEAST | |
Accessioni | P10622Primary (citable) accession number: P10622 Secondary accession number(s): D6VRM0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - Ribosomal proteins
Ribosomal proteins families and list of entries - SIMILARITY comments
Index of protein domains and families