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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanothermus fervidus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits a dual-cofactor specificity, with a marked preference for NADP+ over NAD+.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NADP; via amide nitrogen1 Publication1
Active sitei140NucleophileBy similarity1
Binding sitei171NADP1 Publication1
Binding sitei300NADP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADP1 Publication2
Nucleotide bindingi34 – 35NADP1 Publication2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

SABIO-RKiP10618
UniPathwayi
UPA00109;UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.59)
Short name:
GAPDH
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene namesi
Name:gap
OrganismiMethanothermus fervidus
Taxonomic identifieri2180 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457221 – 337Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST337

Proteomic databases

PRIDEiP10618

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1337
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10618
SMRiP10618
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10618

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493 Archaea
COG0057 LUCA

Family and domain databases

HAMAPiMF_00559 G3P_dehdrog_arch, 1 hit
InterProiView protein in InterPro
IPR000846 DapB_N
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006436 Glyceraldehyde-3-P_DH_2_arc
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PTHR10836:SF76 PTHR10836:SF76, 1 hit
PfamiView protein in Pfam
PF01113 DapB_N, 1 hit
PF02800 Gp_dh_C, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01546 GAPDH-II_archae, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

P10618-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKAVAINGYG TVGKRVADAI AQQDDMKVIG VSKTRPDFEA RMALKKGYDL
60 70 80 90 100
YVAIPERVKL FEKAGIEVAG TVDDMLDEAD IVIDCTPEGI GAKNLKMYKE
110 120 130 140 150
KGIKAIFQGG EKHEDIGLSF NSLSNYEESY GKDYTRVVSC NTTGLCRTLK
160 170 180 190 200
PLHDSFGIKK VRAVIVRRGA DPAQVSKGPI NAIIPNPPKL PSHHGPDVKT
210 220 230 240 250
VLDINIDTMA VIVPTTLMHQ HNVMVEVEET PTVDDIIDVF EDTPRVILIS
260 270 280 290 300
AEDGLTSTAE IMEYAKELGR SRNDLFEIPV WRESITVVDN EIYYMQAVHQ
310 320 330
ESDIVPENVD AVRAILEMEE DKYKSINKTN KAMNILQ
Length:337
Mass (Da):37,408
Last modified:July 1, 1989 - v1
Checksum:iD83CBA88AAE5B0B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19980 Genomic DNA Translation: AAA88227.1
PIRiJT0286

Genome annotation databases

GeneIDi9961991

Similar proteinsi

Entry informationi

Entry nameiG3P_METFE
AccessioniPrimary (citable) accession number: P10618
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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