UniProtKB - P10613 (CP51_CANAL)
Protein
Lanosterol 14-alpha demethylase
Gene
ERG11
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Functioni
Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
Catalytic activityi
- a 14α-methyl steroid + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] = a Δ14 steroid + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH—hemoprotein reductase]EC:1.14.14.154
Cofactori
hemeBy similarity
: zymosterol biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.Proteins known to be involved in the 6 steps of the subpathway in this organism are:
- Lanosterol 14-alpha demethylase (ERG11)
- no protein annotated in this organism
- Methylsterol monooxygenase (ERG25)
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 470 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- monooxygenase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- sterol 14-demethylase activity Source: CGD
GO - Biological processi
- cell growth mode switching, budding to filamentous Source: CGD
- cellular response to drug Source: CGD
- ergosterol biosynthetic process Source: CGD
- membrane raft polarization Source: CGD
- oxidation-reduction process Source: GO_Central
- sterol metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.14.13.70, 1096 |
UniPathwayi | UPA00770;UER00754 |
Names & Taxonomyi
Protein namesi | Recommended name: Lanosterol 14-alpha demethylase (EC:1.14.14.154)Alternative name(s): CYPLI Cytochrome P450 51 Cytochrome P450-14DM Cytochrome P450-LIA1 Sterol 14-alpha demethylase |
Gene namesi | Name:ERG11 Synonyms:CYP51, ERG16 Ordered Locus Names:CAALFM_C500660CA ORF Names:CaO19.922 |
Organismi | Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) |
Taxonomic identifieri | 237561 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Candida/Lodderomyces clade › Candida › |
Proteomesi |
|
Organism-specific databases
CGDi | CAL0000176310, ERG11 |
EuPathDBi | FungiDB:C5_00660C_A |
Subcellular locationi
Other locations
- Membrane Curated
Plasma Membrane
- plasma membrane Source: CGD
Other locations
- integral component of membrane Source: CGD
- membrane Source: CGD
Keywords - Cellular componenti
MembranePathology & Biotechi
Miscellaneous databases
PHI-basei | PHI:8030 PHI:8187 |
Chemistry databases
ChEMBLi | CHEMBL1780 |
DrugBanki | DB04794, Bifonazole DB00257, Clotrimazole DB01127, Econazole DB00196, Fluconazole DB08933, Luliconazole DB01110, Miconazole DB00239, Oxiconazole DB01263, Posaconazole DB01153, Sertaconazole DB00251, Terconazole DB01007, Tioconazole DB00582, Voriconazole |
DrugCentrali | P10613 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000052003 | 1 – 528 | Lanosterol 14-alpha demethylaseAdd BLAST | 528 |
Interactioni
Protein-protein interaction databases
STRINGi | 5476.C4YQW3 |
Chemistry databases
BindingDBi | P10613 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10613 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0684, Eukaryota |
HOGENOMi | CLU_001570_15_0_1 |
InParanoidi | P10613 |
OrthoDBi | 572303at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
P10613-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAIVETVIDG INYFLSLSVT QQISILLGVP FVYNLVWQYL YSLRKDRAPL
60 70 80 90 100
VFYWIPWFGS AASYGQQPYE FFESCRQKYG DVFSFMLLGK IMTVYLGPKG
110 120 130 140 150
HEFVFNAKLS DVSAEDAYKH LTTPVFGKGV IYDCPNSRLM EQKKFAKFAL
160 170 180 190 200
TTDSFKRYVP KIREEILNYF VTDESFKLKE KTHGVANVMK TQPEITIFTA
210 220 230 240 250
SRSLFGDEMR RIFDRSFAQL YSDLDKGFTP INFVFPNLPL PHYWRRDAAQ
260 270 280 290 300
KKISATYMKE IKSRRERGDI DPNRDLIDSL LIHSTYKDGV KMTDQEIANL
310 320 330 340 350
LIGILMGGQH TSASTSAWFL LHLGEKPHLQ DVIYQEVVEL LKEKGGDLND
360 370 380 390 400
LTYEDLQKLP SVNNTIKETL RMHMPLHSIF RKVTNPLRIP ETNYIVPKGH
410 420 430 440 450
YVLVSPGYAH TSERYFDNPE DFDPTRWDTA AAKANSVSFN SSDEVDYGFG
460 470 480 490 500
KVSKGVSSPY LPFGGGRHRC IGEQFAYVQL GTILTTFVYN LRWTIDGYKV
510 520
PDPDYSSMVV LPTEPAEIIW EKRETCMF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | V → D in AAB08099 (PubMed:9210671).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 105 | F → L in fluconazole-resistant isolates. | 1 | |
Natural varianti | 266 | E → D in strain: fluconazole-resistant isolates. | 1 | |
Natural varianti | 287 | K → R in strain: fluconazole-resistant isolates. | 1 | |
Natural varianti | 450 | G → E in strain: fluconazole-resistant isolates. | 1 | |
Natural varianti | 464 | G → S in strain: fluconazole-resistant isolates. | 1 | |
Natural varianti | 467 | R → K in strain: Isolate 13; azole-resistant. 1 Publication | 1 | |
Natural varianti | 488 | V → I in strain: fluconazole-resistant isolates. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13296 Genomic DNA Translation: CAA31658.1 EU819548 Genomic DNA Translation: ACF34636.1 EU819551 Genomic DNA Translation: ACF34639.1 FJ002303 Genomic DNA Translation: ACH91036.1 FJ403378 Genomic DNA Translation: ACJ23064.1 CP017627 Genomic DNA Translation: AOW29509.1 U67192 Genomic DNA Translation: AAB08099.1 U67193 Genomic DNA Translation: AAB08100.1 |
PIRi | S02713, O4CK51 |
RefSeqi | XP_716761.1, XM_711668.2 |
Genome annotation databases
GeneIDi | 3641571 |
KEGGi | cal:CAALFM_C500660CA |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13296 Genomic DNA Translation: CAA31658.1 EU819548 Genomic DNA Translation: ACF34636.1 EU819551 Genomic DNA Translation: ACF34639.1 FJ002303 Genomic DNA Translation: ACH91036.1 FJ403378 Genomic DNA Translation: ACJ23064.1 CP017627 Genomic DNA Translation: AOW29509.1 U67192 Genomic DNA Translation: AAB08099.1 U67193 Genomic DNA Translation: AAB08100.1 |
PIRi | S02713, O4CK51 |
RefSeqi | XP_716761.1, XM_711668.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FSA | X-ray | 2.86 | A/B | 49-528 | [»] | |
5TZ1 | X-ray | 2.00 | A/B | 48-528 | [»] | |
5V5Z | X-ray | 2.90 | A | 1-528 | [»] | |
SMRi | P10613 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 5476.C4YQW3 |
Chemistry databases
BindingDBi | P10613 |
ChEMBLi | CHEMBL1780 |
DrugBanki | DB04794, Bifonazole DB00257, Clotrimazole DB01127, Econazole DB00196, Fluconazole DB08933, Luliconazole DB01110, Miconazole DB00239, Oxiconazole DB01263, Posaconazole DB01153, Sertaconazole DB00251, Terconazole DB01007, Tioconazole DB00582, Voriconazole |
DrugCentrali | P10613 |
Genome annotation databases
GeneIDi | 3641571 |
KEGGi | cal:CAALFM_C500660CA |
Organism-specific databases
CGDi | CAL0000176310, ERG11 |
EuPathDBi | FungiDB:C5_00660C_A |
Phylogenomic databases
eggNOGi | KOG0684, Eukaryota |
HOGENOMi | CLU_001570_15_0_1 |
InParanoidi | P10613 |
OrthoDBi | 572303at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00770;UER00754 |
BRENDAi | 1.14.13.70, 1096 |
Miscellaneous databases
PHI-basei | PHI:8030 PHI:8187 |
PROi | PR:P10613 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP51_CANAL | |
Accessioni | P10613Primary (citable) accession number: P10613 Secondary accession number(s): A0A1D8PMZ1, Q5A524, Q92208 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Candida albicans
Candida albicans: entries and gene names