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Protein

Cathepsin B

Gene

Ctsb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108By similarity1
Active sitei278By similarity1
Active sitei298By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14810
BRENDAi3.4.22.1 3474
3.4.23.5 3474
ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiC01.060

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin B (EC:3.4.22.1)
Alternative name(s):
Cathepsin B1
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:88561 Ctsb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5187

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002614818 – 79Activation peptideAdd BLAST62
ChainiPRO_000002614980 – 333Cathepsin BAdd BLAST254
ChainiPRO_000002615080 – 126Cathepsin B light chainBy similarityAdd BLAST47
ChainiPRO_0000026151129 – 333Cathepsin B heavy chainBy similarityAdd BLAST205
PropeptideiPRO_0000026152334 – 339By similarity6

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 122By similarity
Disulfide bondi105 ↔ 150By similarity
Disulfide bondi141 ↔ 207By similarity
Disulfide bondi142 ↔ 146By similarity
Disulfide bondi179 ↔ 211By similarity
Disulfide bondi187 ↔ 198By similarity
Glycosylationi192N-linked (GlcNAc...) asparagineCurated1
Modified residuei220N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP10605
MaxQBiP10605
PaxDbiP10605
PeptideAtlasiP10605
PRIDEiP10605

2D gel databases

SWISS-2DPAGEiP10605

PTM databases

iPTMnetiP10605
PhosphoSitePlusiP10605
SwissPalmiP10605

Expressioni

Gene expression databases

BgeeiENSMUSG00000021939 Expressed in 320 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_CTSB
GenevisibleiP10605 MM

Interactioni

Subunit structurei

Interacts with SRPX2 (By similarity). Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Directly interacts with SHKBP1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198968, 3 interactors
ComplexPortaliCPX-100 Cathepsin-B - cystatin-A complex
IntActiP10605, 9 interactors
MINTiP10605
STRINGi10090.ENSMUSP00000006235

Chemistry databases

BindingDBiP10605

Structurei

3D structure databases

ProteinModelPortaliP10605
SMRiP10605
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140859
HOGENOMiHOG000241341
HOVERGENiHBG003480
InParanoidiP10605
KOiK01363
OMAiHCGIESS
OrthoDBiEOG091G094Z
PhylomeDBiP10605
TreeFamiTF314576

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR012599 Propeptide_C1A
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00112 Peptidase_C1, 1 hit
PF08127 Propeptide_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10605-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWWSLILLSC LLALTSAHDK PSFHPLSDDL INYINKQNTT WQAGRNFYNV
60 70 80 90 100
DISYLKKLCG TVLGGPKLPG RVAFGEDIDL PETFDAREQW SNCPTIGQIR
110 120 130 140 150
DQGSCGSCWA FGAVEAISDR TCIHTNGRVN VEVSAEDLLT CCGIQCGDGC
160 170 180 190 200
NGGYPSGAWS FWTKKGLVSG GVYNSHVGCL PYTIPPCEHH VNGSRPPCTG
210 220 230 240 250
EGDTPRCNKS CEAGYSPSYK EDKHFGYTSY SVSNSVKEIM AEIYKNGPVE
260 270 280 290 300
GAFTVFSDFL TYKSGVYKHE AGDMMGGHAI RILGWGVENG VPYWLAANSW
310 320 330
NLDWGDNGFF KILRGENHCG IESEIVAGIP RTDQYWGRF
Length:339
Mass (Da):37,280
Last modified:February 1, 1991 - v2
Checksum:i9F0A3CDF70A94040
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160S → N in AAA37494 (PubMed:3463996).Curated1
Sequence conflicti174N → D in AAA37494 (PubMed:3463996).Curated1
Sequence conflicti177V → I in AAA37494 (PubMed:3463996).Curated1
Sequence conflicti284G → V in AAA37494 (PubMed:3463996).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65270
, M65263, M65264, M65265, M65266, M65267, M65268, M65269 Genomic DNA Translation: AAA37375.1
M14222 mRNA Translation: AAA37494.1
X54966 mRNA Translation: CAA38713.1
S69034 mRNA Translation: AAB20536.1
AK083393 mRNA Translation: BAC38900.1
AK147192 mRNA Translation: BAE27751.1
AK149884 mRNA Translation: BAE29144.1
AK151790 mRNA Translation: BAE30691.1
AK167361 mRNA Translation: BAE39458.1
BC006656 mRNA Translation: AAH06656.1
CCDSiCCDS27197.1
PIRiA38458 KHMSB
RefSeqiNP_031824.1, NM_007798.3
UniGeneiMm.236553

Genome annotation databases

EnsembliENSMUST00000006235; ENSMUSP00000006235; ENSMUSG00000021939
GeneIDi13030
KEGGimmu:13030
UCSCiuc007uhh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65270
, M65263, M65264, M65265, M65266, M65267, M65268, M65269 Genomic DNA Translation: AAA37375.1
M14222 mRNA Translation: AAA37494.1
X54966 mRNA Translation: CAA38713.1
S69034 mRNA Translation: AAB20536.1
AK083393 mRNA Translation: BAC38900.1
AK147192 mRNA Translation: BAE27751.1
AK149884 mRNA Translation: BAE29144.1
AK151790 mRNA Translation: BAE30691.1
AK167361 mRNA Translation: BAE39458.1
BC006656 mRNA Translation: AAH06656.1
CCDSiCCDS27197.1
PIRiA38458 KHMSB
RefSeqiNP_031824.1, NM_007798.3
UniGeneiMm.236553

3D structure databases

ProteinModelPortaliP10605
SMRiP10605
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198968, 3 interactors
ComplexPortaliCPX-100 Cathepsin-B - cystatin-A complex
IntActiP10605, 9 interactors
MINTiP10605
STRINGi10090.ENSMUSP00000006235

Chemistry databases

BindingDBiP10605
ChEMBLiCHEMBL5187

Protein family/group databases

MEROPSiC01.060

PTM databases

iPTMnetiP10605
PhosphoSitePlusiP10605
SwissPalmiP10605

2D gel databases

SWISS-2DPAGEiP10605

Proteomic databases

EPDiP10605
MaxQBiP10605
PaxDbiP10605
PeptideAtlasiP10605
PRIDEiP10605

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006235; ENSMUSP00000006235; ENSMUSG00000021939
GeneIDi13030
KEGGimmu:13030
UCSCiuc007uhh.2 mouse

Organism-specific databases

CTDi1508
MGIiMGI:88561 Ctsb

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140859
HOGENOMiHOG000241341
HOVERGENiHBG003480
InParanoidiP10605
KOiK01363
OMAiHCGIESS
OrthoDBiEOG091G094Z
PhylomeDBiP10605
TreeFamiTF314576

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14810
BRENDAi3.4.22.1 3474
3.4.23.5 3474
ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiCtsb mouse
PROiPR:P10605
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021939 Expressed in 320 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_CTSB
GenevisibleiP10605 MM

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR012599 Propeptide_C1A
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00112 Peptidase_C1, 1 hit
PF08127 Propeptide_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCATB_MOUSE
AccessioniPrimary (citable) accession number: P10605
Secondary accession number(s): Q3UDW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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