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Protein

Nidogen-1

Gene

Nid1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei457Involved in perlecan binding1
Sitei459Involved in perlecan binding1
Sitei648Involved in perlecan binding1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-3000157 Laminin interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Nidogen-1
Short name:
NID-1
Alternative name(s):
Entactin
Gene namesi
Name:Nid1
Synonyms:Ent
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97342 Nid1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000000767029 – 1245Nidogen-1Add BLAST1217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi187N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei290SulfotyrosineSequence analysis1
Modified residuei295SulfotyrosineSequence analysis1
Glycosylationi299O-linked (GalNAc...) threonine1 Publication1
Glycosylationi331O-linked (GalNAc...) serine1 Publication1
Glycosylationi337O-linked (GalNAc...) threonine1 Publication1
Glycosylationi345O-linked (GalNAc...) threonine1 Publication1
Glycosylationi348O-linked (GalNAc...) threonine; partial1 Publication1
Disulfide bondi388 ↔ 401
Disulfide bondi395 ↔ 410
Disulfide bondi409 ↔ 616
Disulfide bondi412 ↔ 423
Glycosylationi415N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi670 ↔ 683By similarity
Disulfide bondi677 ↔ 693By similarity
Disulfide bondi695 ↔ 706By similarity
Disulfide bondi712 ↔ 725By similarity
Disulfide bondi719 ↔ 734By similarity
Disulfide bondi736 ↔ 748By similarity
Disulfide bondi760 ↔ 775By similarity
Disulfide bondi767 ↔ 785By similarity
Disulfide bondi787 ↔ 798By similarity
Disulfide bondi804 ↔ 815By similarity
Disulfide bondi809 ↔ 824By similarity
Disulfide bondi826 ↔ 837By similarity
Disulfide bondi847 ↔ 876By similarity
Disulfide bondi887 ↔ 894By similarity
Disulfide bondi896 ↔ 917By similarity
Glycosylationi920O-linked (GalNAc...) threonine1 Publication1
Glycosylationi933O-linked (GalNAc...) threonine1 Publication1
Disulfide bondi1210 ↔ 1221By similarity
Disulfide bondi1217 ↔ 1230By similarity
Disulfide bondi1232 ↔ 1241By similarity

Post-translational modificationi

N- and O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

MaxQBiP10493
PaxDbiP10493
PeptideAtlasiP10493
PRIDEiP10493

PTM databases

iPTMnetiP10493
PhosphoSitePlusiP10493

Miscellaneous databases

PMAP-CutDBiP10493

Expressioni

Gene expression databases

BgeeiENSMUSG00000005397 Expressed in 286 organ(s), highest expression level in endothelial cell of lymphatic vessel
CleanExiMM_NID1
GenevisibleiP10493 MM

Interactioni

Subunit structurei

Interacts with FBLN1 and LGALS3BP. Interacts with PLXDC1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Lamc1P024685EBI-1032117,EBI-7059830

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201770, 5 interactors
ComplexPortaliCPX-1281 Laminin111-nidogen complex
CPX-1284 Laminin211-nidogen complex
CPX-1286 Laminin221-nidogen complex
IntActiP10493, 6 interactors
MINTiP10493
STRINGi10090.ENSMUSP00000005532

Structurei

Secondary structure

11245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10493
SMRiP10493
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10493

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 268NIDOPROSITE-ProRule annotationAdd BLAST163
Domaini384 – 424EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini428 – 665Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd BLAST238
Domaini666 – 707EGF-like 2PROSITE-ProRule annotationAdd BLAST42
Domaini708 – 749EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini756 – 799EGF-like 4PROSITE-ProRule annotationAdd BLAST44
Domaini800 – 838EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini844 – 917Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST74
Repeati988 – 1030LDL-receptor class B 1Add BLAST43
Repeati1031 – 1073LDL-receptor class B 2Add BLAST43
Repeati1074 – 1118LDL-receptor class B 3Add BLAST45
Repeati1119 – 1160LDL-receptor class B 4Add BLAST42
Domaini1206 – 1242EGF-like 6PROSITE-ProRule annotationAdd BLAST37

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi700 – 702Cell attachment site3

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1214 Eukaryota
ENOG410XR8N LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiP10493
KOiK06826
OMAiIGVAYDC
OrthoDBiEOG091G030P
TreeFamiTF320666

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 1 hit
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR026823 cEGF
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR024731 EGF_dom
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF12662 cEGF, 1 hit
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 3 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 6 hits
SM00179 EGF_CA, 4 hits
SM00682 G2F, 1 hit
SM00135 LY, 5 hits
SM00539 NIDO, 1 hit
SM00211 TY, 1 hit
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 1 hit
SSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 4 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10493-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLDASGCSWA MWTWALLQLL LLVGPGGCLN RQELFPFGPG QGDLELEAGD
60 70 80 90 100
DVVSPSLELI GELSFYDRTD ITSVYVTTNG IIAMSEPPAT EYHPGTFPPS
110 120 130 140 150
FGSVAPFLAD LDTTDGLGNV YYREDLSPFI IQMAAEYVQR GFPEVSFQPT
160 170 180 190 200
SVVVVTWESV APYGGPSSSP AEEGKRNTFQ AVLASSNSSS YAIFLYPEDG
210 220 230 240 250
LQFFTTFSKK DESQVPAVVG FSKGLVGFLW KSNGAYNIFA NDRESIENLA
260 270 280 290 300
KSSNAGHQGV WVFEIGSPAT AKGVVSADVN LDLDDDGADY EDEDYDLVTS
310 320 330 340 350
HLGLEDVATP SPSHSPRRGY PDPHNVPRIL SPGYEATERP RGVPTERTRS
360 370 380 390 400
FQLPAERFPQ HHPQVIDVDE VEETGVVFSY NTGSQQTCAN NRHQCSVHAE
410 420 430 440 450
CRDYATGFCC RCVANYTGNG RQCVAEGSPQ RVNGKVKGRI FVGSSQVPVV
460 470 480 490 500
FENTDLHSYV VMNHGRSYTA ISTIPETVGY SLLPLAPIGG IIGWMFAVEQ
510 520 530 540 550
DGFKNGFSIT GGEFTRQAEV TFLGHPGKLV LKQQFSGIDE HGHLTISTEL
560 570 580 590 600
EGRVPQIPYG ASVHIEPYTE LYHYSSSVIT SSSTREYTVM EPDQDGAAPS
610 620 630 640 650
HTHIYQWRQT ITFQECAHDD ARPALPSTQQ LSVDSVFVLY NKEERILRYA
660 670 680 690 700
LSNSIGPVRD GSPDALQNPC YIGTHGCDSN AACRPGPGTQ FTCECSIGFR
710 720 730 740 750
GDGQTCYDID ECSEQPSRCG NHAVCNNLPG TFRCECVEGY HFSDRGTCVA
760 770 780 790 800
AEDQRPINYC ETGLHNCDIP QRAQCIYMGG SSYTCSCLPG FSGDGRACRD
810 820 830 840 850
VDECQHSRCH PDAFCYNTPG SFTCQCKPGY QGDGFRCMPG EVSKTRCQLE
860 870 880 890 900
REHILGAAGG ADAQRPTLQG MFVPQCDEYG HYVPTQCHHS TGYCWCVDRD
910 920 930 940 950
GRELEGSRTP PGMRPPCLST VAPPIHQGPV VPTAVIPLPP GTHLLFAQTG
960 970 980 990 1000
KIERLPLERN TMKKTEAKAF LHIPAKVIIG LAFDCVDKVV YWTDISEPSI
1010 1020 1030 1040 1050
GRASLHGGEP TTIIRQDLGS PEGIALDHLG RTIFWTDSQL DRIEVAKMDG
1060 1070 1080 1090 1100
TQRRVLFDTG LVNPRGIVTD PVRGNLYWTD WNRDNPKIET SHMDGTNRRI
1110 1120 1130 1140 1150
LAQDNLGLPN GLTFDAFSSQ LCWVDAGTHR AECLNPAQPG RRKVLEGLQY
1160 1170 1180 1190 1200
PFAVTSYGKN LYYTDWKTNS VIAMDLAISK EMDTFHPHKQ TRLYGITIAL
1210 1220 1230 1240
SQCPQGHNYC SVNNGGCTHL CLPTPGSRTC RCPDNTLGVD CIERK
Length:1,245
Mass (Da):136,538
Last modified:July 27, 2011 - v2
Checksum:i92D12D128E6EF144
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170P → L in CAA32642 (PubMed:2496973).Curated1
Sequence conflicti659R → K in CAA32642 (PubMed:2496973).Curated1
Sequence conflicti967A → R in CAA32408 (PubMed:3264556).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14194 mRNA Translation: CAA32408.1
X14480 mRNA Translation: CAA32642.1
AK041633 mRNA Translation: BAC31014.1
AK084876 mRNA Translation: BAC39300.1
AK144878 mRNA Translation: BAE26114.1
AK166779 mRNA Translation: BAE39014.1
BC131669 mRNA Translation: AAI31670.1
AH003206 Genomic DNA Translation: AAA77652.1
X83093 Genomic DNA Translation: CAA58148.1
CCDSiCCDS26244.1
PIRiS02730 MMMSND
RefSeqiNP_035047.2, NM_010917.2
UniGeneiMm.4691

Genome annotation databases

EnsembliENSMUST00000005532; ENSMUSP00000005532; ENSMUSG00000005397
GeneIDi18073
KEGGimmu:18073
UCSCiuc007pmf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14194 mRNA Translation: CAA32408.1
X14480 mRNA Translation: CAA32642.1
AK041633 mRNA Translation: BAC31014.1
AK084876 mRNA Translation: BAC39300.1
AK144878 mRNA Translation: BAE26114.1
AK166779 mRNA Translation: BAE39014.1
BC131669 mRNA Translation: AAI31670.1
AH003206 Genomic DNA Translation: AAA77652.1
X83093 Genomic DNA Translation: CAA58148.1
CCDSiCCDS26244.1
PIRiS02730 MMMSND
RefSeqiNP_035047.2, NM_010917.2
UniGeneiMm.4691

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GL4X-ray2.00A385-665[»]
1H4UX-ray2.20A395-659[»]
1NPEX-ray2.30A941-1207[»]
ProteinModelPortaliP10493
SMRiP10493
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201770, 5 interactors
ComplexPortaliCPX-1281 Laminin111-nidogen complex
CPX-1284 Laminin211-nidogen complex
CPX-1286 Laminin221-nidogen complex
IntActiP10493, 6 interactors
MINTiP10493
STRINGi10090.ENSMUSP00000005532

PTM databases

iPTMnetiP10493
PhosphoSitePlusiP10493

Proteomic databases

MaxQBiP10493
PaxDbiP10493
PeptideAtlasiP10493
PRIDEiP10493

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005532; ENSMUSP00000005532; ENSMUSG00000005397
GeneIDi18073
KEGGimmu:18073
UCSCiuc007pmf.2 mouse

Organism-specific databases

CTDi4811
MGIiMGI:97342 Nid1

Phylogenomic databases

eggNOGiKOG1214 Eukaryota
ENOG410XR8N LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiP10493
KOiK06826
OMAiIGVAYDC
OrthoDBiEOG091G030P
TreeFamiTF320666

Enzyme and pathway databases

ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-3000157 Laminin interactions

Miscellaneous databases

ChiTaRSiNid1 mouse
EvolutionaryTraceiP10493
PMAP-CutDBiP10493
PROiPR:P10493
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005397 Expressed in 286 organ(s), highest expression level in endothelial cell of lymphatic vessel
CleanExiMM_NID1
GenevisibleiP10493 MM

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 1 hit
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR026823 cEGF
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR024731 EGF_dom
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF12662 cEGF, 1 hit
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 3 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 6 hits
SM00179 EGF_CA, 4 hits
SM00682 G2F, 1 hit
SM00135 LY, 5 hits
SM00539 NIDO, 1 hit
SM00211 TY, 1 hit
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 1 hit
SSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 4 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNID1_MOUSE
AccessioniPrimary (citable) accession number: P10493
Secondary accession number(s): Q3TKX9
, Q8BQI3, Q8C3U8, Q8C9P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 198 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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