UniProtKB - P10476 (GUNA_CELJU)
Protein
Endoglucanase A
Gene
celA
Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 203 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 523 | PROSITE-ProRule annotation | 1 | |
Active sitei | 573 | PROSITE-ProRule annotation | 1 | |
Active sitei | 582 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
- cellulose binding Source: InterPro
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Enzyme and pathway databases
BioCyci | CJAP498211:G1GB3-2449-MONOMER |
Protein family/group databases
CAZyi | CBM10, Carbohydrate-Binding Module Family 10 CBM2, Carbohydrate-Binding Module Family 2 GH9, Glycoside Hydrolase Family 9 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase A (EC:3.2.1.4)Short name: EGA Alternative name(s): Cellulase Endo-1,4-beta-glucanase |
Gene namesi | Name:celA Synonyms:cel9A Ordered Locus Names:CJA_2472 |
Organismi | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
Taxonomic identifieri | 498211 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Cellvibrio › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000007957 | 33 – 962 | Endoglucanase AAdd BLAST | 930 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 671 ↔ 702 | By similarity | ||
Disulfide bondi | 681 ↔ 696 | By similarity | ||
Disulfide bondi | 866 ↔ 961 | By similarity |
Keywords - PTMi
Disulfide bondFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 670 – 699 | CBM10PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 859 – 962 | CBM2PROSITE-ProRule annotationAdd BLAST | 104 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 608 – 664 | Ser-richAdd BLAST | 57 | |
Compositional biasi | 823 – 859 | Ser-rich (linker)Add BLAST | 37 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3291, Bacteria COG4447, Bacteria COG5520, Bacteria |
HOGENOMi | CLU_006010_3_0_6 |
OrthoDBi | 1226595at2 |
Family and domain databases
CDDi | cd02850, E_set_Cellulase_N, 1 hit |
Gene3Di | 1.50.10.10, 1 hit 2.30.32.30, 1 hit 2.60.40.10, 2 hits 2.60.40.290, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001919, CBD2 IPR002883, CBM10/Dockerin_dom IPR036601, CBM10_sf IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR009031, CBM_fam10 IPR004197, Cellulase_Ig-like IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR000601, PKD_dom IPR035986, PKD_dom_sf |
Pfami | View protein in Pfam PF02013, CBM_10, 1 hit PF00553, CBM_2, 1 hit PF02927, CelD_N, 1 hit PF00759, Glyco_hydro_9, 1 hit PF00801, PKD, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit SM01064, CBM_10, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49299, SSF49299, 1 hit SSF49384, SSF49384, 1 hit SSF57615, SSF57615, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS51763, CBM10, 1 hit PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MINRSVLKIP ALVKPLVQAL VLVGCTLGVA QAEVGNPRVN QLGYIPNGDR
60 70 80 90 100
IAVYKASNNS AQTWQLTHNG SLIASGQTIP KGSDASSGDN IHHIDLSSVT
110 120 130 140 150
ATGSGFTLTV GGDSSYPFSI SSTTFNAAFY DALKYFYHNR SGIAIETPYT
160 170 180 190 200
GGGRGSYASH SRWSRPAGHL NQGANKGDMN VPCWSGTCNY SLNVTKGWYD
210 220 230 240 250
AGDHGKYVVN GGISVWTLLN LYERAQHITG NLAAVADGSM NIPESGNGVA
260 270 280 290 300
DILDEARWQM EFMLAMQVPQ GQAKAGMAHH KIHDVGWTGL PLAPHEDPQQ
310 320 330 340 350
RALVPPSTAA TLNLAATAAQ AARIWKDIDA GFAALCLTAA ERAWNAAQAN
360 370 380 390 400
PNDIYSGNYD NGGGGYGDRF VADEFYWAAA ELYITTGDSR YLPTINNYTL
410 420 430 440 450
ERTDFGWPDT ELLGVMSLAV VPATHTNSLR IAARNHIQTI ASTHLTTQSA
460 470 480 490 500
SGYPAPLSSL EYYWGSNSVI ANKLVLMGLA YDFSGNQNFA LGVSKGINYL
510 520 530 540 550
FGSNVLSTSF ITGLGTNTVA QPHHRFWAGA LNSNYPWAPP GALSGGPNAG
560 570 580 590 600
LEDSLSASRL SGCTSRPATC WLDSIDAWST NEITINWNAP LAWVLGFYND
610 620 630 640 650
FAATQGGSSS SSSSSSSSVP VSSSSSSSII PSSSSSSIQP SSSSSSMPSS
660 670 680 690 700
SSSSSSVVAS SSSSVSGGLR CNWYGTLYPL CVTTQSGWGW ENSQSCISAS
710 720 730 740 750
TCSAQPAPYG IVGAASSSSQ AANRSPTLQL SANATGFEGG SMVCCTLHIN
760 770 780 790 800
GAASDPDGDN LTYSWQVISG NTVVASGSSS SASIHVSNQR GYEVSMTVSD
810 820 830 840 850
GRGGVATETT FVSVYFSDYF PGSSSSASNI NSSSSSSSSS SSSAIVSSSS
860 870 880 890 900
SVVSSSSSSA ASGGNCQYVV TNQWNNGFTA VIRVRNNGSS AINGWSVNWS
910 920 930 940 950
YSDGSRITNS WNANVTGNNP YAASALGWNA NIQPGQTAEF GFQGTKGAGS
960
AQVPAVTGSV CQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | K → T in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 25 | C → G in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 503 | S → I in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 507 | S → P in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 555 | L → F in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 894 | G → R in CAA31082 (PubMed:2851699).Curated | 1 | |
Sequence conflicti | 951 | A → R in CAA31082 (PubMed:2851699).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12570 Genomic DNA Translation: CAA31082.1 CP000934 Genomic DNA Translation: ACE85757.1 |
RefSeqi | WP_012488069.1, NC_010995.1 |
Genome annotation databases
EnsemblBacteriai | ACE85757; ACE85757; CJA_2472 |
KEGGi | cja:CJA_2472 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12570 Genomic DNA Translation: CAA31082.1 CP000934 Genomic DNA Translation: ACE85757.1 |
RefSeqi | WP_012488069.1, NC_010995.1 |
3D structure databases
SMRi | P10476 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 498211.CJA_2472 |
Protein family/group databases
CAZyi | CBM10, Carbohydrate-Binding Module Family 10 CBM2, Carbohydrate-Binding Module Family 2 GH9, Glycoside Hydrolase Family 9 |
Genome annotation databases
EnsemblBacteriai | ACE85757; ACE85757; CJA_2472 |
KEGGi | cja:CJA_2472 |
Phylogenomic databases
eggNOGi | COG3291, Bacteria COG4447, Bacteria COG5520, Bacteria |
HOGENOMi | CLU_006010_3_0_6 |
OrthoDBi | 1226595at2 |
Enzyme and pathway databases
BioCyci | CJAP498211:G1GB3-2449-MONOMER |
Family and domain databases
CDDi | cd02850, E_set_Cellulase_N, 1 hit |
Gene3Di | 1.50.10.10, 1 hit 2.30.32.30, 1 hit 2.60.40.10, 2 hits 2.60.40.290, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001919, CBD2 IPR002883, CBM10/Dockerin_dom IPR036601, CBM10_sf IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR009031, CBM_fam10 IPR004197, Cellulase_Ig-like IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR000601, PKD_dom IPR035986, PKD_dom_sf |
Pfami | View protein in Pfam PF02013, CBM_10, 1 hit PF00553, CBM_2, 1 hit PF02927, CelD_N, 1 hit PF00759, Glyco_hydro_9, 1 hit PF00801, PKD, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit SM01064, CBM_10, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49299, SSF49299, 1 hit SSF49384, SSF49384, 1 hit SSF57615, SSF57615, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS51763, CBM10, 1 hit PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNA_CELJU | |
Accessioni | P10476Primary (citable) accession number: P10476 Secondary accession number(s): B3PKK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | April 20, 2010 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families