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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.1 Publication

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • lipid-A-disaccharide synthase activity Source: EcoliWiki
  • phospholipid binding Source: EcoliWiki

GO - Biological processi

  • lipid A biosynthetic process Source: EcoliWiki

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER
UniPathwayi
UPA00359;UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.1821 Publication)
Gene namesi
Name:lpxB
Synonyms:pgsB
Ordered Locus Names:b0182, JW0177
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10546 lpxB

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901641 – 382Lipid-A-disaccharide synthaseAdd BLAST382

Proteomic databases

EPDiP10441
PaxDbiP10441
PRIDEiP10441

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tufAP0CE473EBI-553692,EBI-301077

Protein-protein interaction databases

BioGridi4260870, 581 interactors
DIPiDIP-10122N
IntActiP10441, 26 interactors
STRINGi316385.ECDH10B_0162

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10441
SMRiP10441
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
InParanoidiP10441
KOiK00748
OMAiPTVWAWR
PhylomeDBiP10441

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

P10441-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG
60 70 80 90 100
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP
110 120 130 140 150
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE
160 170 180 190 200
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS
210 220 230 240 250
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP
260 270 280 290 300
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF
310 320 330 340 350
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT
360 370 380
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ
Length:382
Mass (Da):42,382
Last modified:July 19, 2003 - v2
Checksum:i12C76B6ECA438B47
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149F → V (PubMed:2824445).Curated1
Sequence conflicti149F → V (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA Translation: AAC36919.1
U70214 Genomic DNA Translation: AAB08611.1
U00096 Genomic DNA Translation: AAC73293.1
AP009048 Genomic DNA Translation: BAA77857.2
PIRiF64742 SYECLA
RefSeqiNP_414724.1, NC_000913.3
WP_000139654.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73293; AAC73293; b0182
BAA77857; BAA77857; BAA77857
GeneIDi944838
KEGGiecj:JW0177
eco:b0182
PATRICifig|1411691.4.peg.2097

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA Translation: AAC36919.1
U70214 Genomic DNA Translation: AAB08611.1
U00096 Genomic DNA Translation: AAC73293.1
AP009048 Genomic DNA Translation: BAA77857.2
PIRiF64742 SYECLA
RefSeqiNP_414724.1, NC_000913.3
WP_000139654.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W8NX-ray2.02A1-382[»]
5W8SX-ray2.10A1-382[»]
5W8XX-ray1.98A1-382[»]
ProteinModelPortaliP10441
SMRiP10441
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260870, 581 interactors
DIPiDIP-10122N
IntActiP10441, 26 interactors
STRINGi316385.ECDH10B_0162

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Proteomic databases

EPDiP10441
PaxDbiP10441
PRIDEiP10441

Protocols and materials databases

DNASUi944838
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73293; AAC73293; b0182
BAA77857; BAA77857; BAA77857
GeneIDi944838
KEGGiecj:JW0177
eco:b0182
PATRICifig|1411691.4.peg.2097

Organism-specific databases

EchoBASEiEB0541
EcoGeneiEG10546 lpxB

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
InParanoidiP10441
KOiK00748
OMAiPTVWAWR
PhylomeDBiP10441

Enzyme and pathway databases

UniPathwayi
UPA00359;UER00481

BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER

Miscellaneous databases

PROiPR:P10441

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLPXB_ECOLI
AccessioniPrimary (citable) accession number: P10441
Secondary accession number(s): P78298
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2003
Last modified: July 18, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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