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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for protein export, interacts with the SecYEG preprotein conducting channel. SecA has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit. Zinc is required for binding of the SecB chaperone to the extreme C-terminus of SecA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi885Zinc1
Metal bindingi887Zinc1
Metal bindingi896Zinc1
Metal bindingi897Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 109ATPSequence analysis8

GO - Molecular functioni

  • ATPase-coupled protein transmembrane transporter activity Source: EcoCyc
  • ATP binding Source: GO_Central
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:SECA
BRENDAi3.6.3.B1 2026

Protein family/group databases

TCDBi3.A.5.1.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecA
Gene namesi
Name:secA
Synonyms:azi, pea, prlD
Ordered Locus Names:b0098, JW0096
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10936 secA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential. Leads to loss of translocation of lipoproteins Lpp and BRP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095851 – 901Protein translocase subunit SecAAdd BLAST901

Proteomic databases

EPDiP10408
PaxDbiP10408
PRIDEiP10408

Expressioni

Inductioni

Repressed under conditions of excess protein secretion capacity and derepressed when protein secretion becomes limiting. This is regulated by SecM.

Interactioni

Subunit structurei

Monomer and homodimer; probably dimeric in the cytoplasm. It is not yet clear whether the form that functions in protein insertion is monomeric or dimeric. Part of the essential protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC. Makes direct contact with SecY.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261117, 260 interactors
849222, 1 interactor
DIPiDIP-10840N
IntActiP10408, 86 interactors
MINTiP10408
STRINGi316385.ECDH10B_0080

Structurei

Secondary structure

1901
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10408
SMRiP10408
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10408

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI6 Bacteria
COG0653 LUCA
HOGENOMiHOG000218168
InParanoidiP10408
KOiK03070
OMAiFEYDEVM
PhylomeDBiP10408

Family and domain databases

HAMAPiMF_01382 SecA, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR004027 SEC_C_motif
IPR000185 SecA
IPR020937 SecA_CS
IPR011115 SecA_DEAD
IPR014018 SecA_motor_DEAD
IPR011130 SecA_preprotein_X-link_dom
IPR011116 SecA_Wing/Scaffold
IPR036266 SecA_Wing/Scaffold_sf
IPR036670 SecA_X-link_sf
PANTHERiPTHR30612 PTHR30612, 1 hit
PfamiView protein in Pfam
PF02810 SEC-C, 1 hit
PF07517 SecA_DEAD, 1 hit
PF01043 SecA_PP_bind, 1 hit
PF07516 SecA_SW, 1 hit
PRINTSiPR00906 SECA
SMARTiView protein in SMART
SM00957 SecA_DEAD, 1 hit
SM00958 SecA_PP_bind, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
SSF81767 SSF81767, 1 hit
SSF81886 SSF81886, 2 hits
TIGRFAMsiTIGR00963 secA, 1 hit
PROSITEiView protein in PROSITE
PS01312 SECA, 1 hit
PS51196 SECA_MOTOR_DEAD, 1 hit

Sequencei

Sequence statusi: Complete.

P10408-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF
60 70 80 90 100
RARLEKGEVL ENLIPEAFAV VREASKRVFG MRHFDVQLLG GMVLNERCIA
110 120 130 140 150
EMRTGEGKTL TATLPAYLNA LTGKGVHVVT VNDYLAQRDA ENNRPLFEFL
160 170 180 190 200
GLTVGINLPG MPAPAKREAY AADITYGTNN EYGFDYLRDN MAFSPEERVQ
210 220 230 240 250
RKLHYALVDE VDSILIDEAR TPLIISGPAE DSSEMYKRVN KIIPHLIRQE
260 270 280 290 300
KEDSETFQGE GHFSVDEKSR QVNLTERGLV LIEELLVKEG IMDEGESLYS
310 320 330 340 350
PANIMLMHHV TAALRAHALF TRDVDYIVKD GEVIIVDEHT GRTMQGRRWS
360 370 380 390 400
DGLHQAVEAK EGVQIQNENQ TLASITFQNY FRLYEKLAGM TGTADTEAFE
410 420 430 440 450
FSSIYKLDTV VVPTNRPMIR KDLPDLVYMT EAEKIQAIIE DIKERTAKGQ
460 470 480 490 500
PVLVGTISIE KSELVSNELT KAGIKHNVLN AKFHANEAAI VAQAGYPAAV
510 520 530 540 550
TIATNMAGRG TDIVLGGSWQ AEVAALENPT AEQIEKIKAD WQVRHDAVLE
560 570 580 590 600
AGGLHIIGTE RHESRRIDNQ LRGRSGRQGD AGSSRFYLSM EDALMRIFAS
610 620 630 640 650
DRVSGMMRKL GMKPGEAIEH PWVTKAIANA QRKVESRNFD IRKQLLEYDD
660 670 680 690 700
VANDQRRAIY SQRNELLDVS DVSETINSIR EDVFKATIDA YIPPQSLEEM
710 720 730 740 750
WDIPGLQERL KNDFDLDLPI AEWLDKEPEL HEETLRERIL AQSIEVYQRK
760 770 780 790 800
EEVVGAEMMR HFEKGVMLQT LDSLWKEHLA AMDYLRQGIH LRGYAQKDPK
810 820 830 840 850
QEYKRESFSM FAAMLESLKY EVISTLSKVQ VRMPEEVEEL EQQRRMEAER
860 870 880 890 900
LAQMQQLSHQ DDDSAAAAAL AAQTGERKVG RNDPCPCGSG KKYKQCHGRL

Q
Length:901
Mass (Da):102,023
Last modified:November 1, 1997 - v2
Checksum:i683D66DD4305DBC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19R → G AA sequence (PubMed:2841285).Curated1
Sequence conflicti737 – 738ER → DG in AAA24619 (PubMed:2841285).Curated2
Sequence conflicti737 – 738ER → DG in CAA38875 (PubMed:1630901).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20791 Genomic DNA Translation: AAA24619.1
M19211 Genomic DNA Translation: AAA83851.1
X55034 Genomic DNA Translation: CAA38875.1
U00096 Genomic DNA Translation: AAC73209.1
AP009048 Genomic DNA Translation: BAB96666.2
PIRiB64732 BVECCA
RefSeqiNP_414640.1, NC_000913.3
WP_000905789.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73209; AAC73209; b0098
BAB96666; BAB96666; BAB96666
GeneIDi944821
KEGGiecj:JW0096
eco:b0098
PATRICifig|1411691.4.peg.2182

Similar proteinsi

Entry informationi

Entry nameiSECA_ECOLI
AccessioniPrimary (citable) accession number: P10408
Secondary accession number(s): P75642
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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