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Entry version 93 (11 Dec 2019)
Sequence version 1 (01 Jul 1989)
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Protein

FeMo cofactor biosynthesis protein NifB

Gene

nifB

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co or M-cluster) found in the dinitrogenase enzyme of the nitrogenase complex in nitrogen-fixing microorganisms. NifB catalyzes the crucial step of radical SAM-dependent carbide insertion that occurs concomitant with the insertion of a 9th sulfur and the rearrangement/coupling of two [4Fe-4S] clusters into a [8Fe-9S-C] cluster, the precursor to the M-cluster.By similarity

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per monomer. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. The two others probably act as substrate.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Fe-Mo cofactor biosynthesis

This protein is involved in the pathway Fe-Mo cofactor biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway Fe-Mo cofactor biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi54Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi58Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei60S-adenosyl-L-methionineBy similarity1
Metal bindingi61Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Binding sitei108S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei160S-adenosyl-L-methionineBy similarity1
Binding sitei212S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi285Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi288Iron-sulfur 2 (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase
Biological processNitrogen fixation
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00782

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
FeMo cofactor biosynthesis protein NifB (EC:4.-.-.-)
Alternative name(s):
Nitrogenase cofactor maturase NifB
Radical SAM assemblase NifB
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nifB
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiKlebsiella pneumoniae
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri573 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001530411 – 468FeMo cofactor biosynthesis protein NifBAdd BLAST468

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the radical SAM superfamily. NifB family.Curated

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00852 NifB, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.20.20.70, 1 hit
3.30.420.130, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013785 Aldolase_TIM
IPR003731 Di-Nase_FeMo-co_biosynth
IPR036105 DiNase_FeMo-co_biosyn_sf
IPR006638 Elp3/MiaB/NifB
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR005980 Nase_CF_NifB
IPR034165 NifB_C
IPR007197 rSAM

The PANTHER Classification System

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PANTHERi
PTHR43787:SF9 PTHR43787:SF9, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF02579 Nitro_FeMo-Co, 1 hit
PF04055 Radical_SAM, 1 hit

Structure-Function Linkage Database

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SFLDi
SFLDS00029 Radical_SAM, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00729 Elp3, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53146 SSF53146, 1 hit

TIGRFAMs; a protein family database

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TIGRFAMsi
TIGR01290 nifB, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P10390-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSCSSFSGG KACRPADDSA LTPLVADKAA AHPCYSRHGH HRFARMHLPV
60 70 80 90 100
APACNLQCNY CNRKFDCSNE SRPGVSSTLL TPEQAVVKVR QVAQAIPQLS
110 120 130 140 150
VVGIAGPGDP LANIARTFRT LELIREQLPD LKLCLSTNGL VLPDAVDRLL
160 170 180 190 200
DVGVDHVTVT INTLDAEIAA QIYAWLWLDG ERYSGREAGE ILIARQLEGV
210 220 230 240 250
RRLTAKGVLV KINSVLIPGI NDSGMAGVSR ALRASGAFIH NIMPLIARPE
260 270 280 290 300
HGTVFGLNGQ PEPDAETLAA TRSRCGEVMP QMTHCHQCRA DAIGMLGEDR
310 320 330 340 350
SQQFTQLPAP ESLPAWLPIL HQRAQLHASI ATRGESEADD ACLVAVASSR
360 370 380 390 400
GDVIDCHFGH ADRFYIYSLS AAGMVLVNER FTPKYCQGRD DCEPQDNAAR
410 420 430 440 450
FAAILELLAD VKAVFCVRIG HTPWQQLEQE GIEPCVDGAW RPVSEVLPAW
460
WQQRRGSWPA ALPHKGVA
Length:468
Mass (Da):50,856
Last modified:July 1, 1989 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i89BF828E3E73FE1E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti141V → M in AAA25107 (PubMed:3029020).Curated1
Sequence conflicti188A → G in AAA25107 (PubMed:3029020).Curated1
Sequence conflicti227G → D in AAA25107 (PubMed:3029020).Curated1
Sequence conflicti256G → R in AAA25107 (PubMed:3029020).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X13303 Genomic DNA Translation: CAA31683.1
M15545 Genomic DNA Translation: AAA25107.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S02514

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13303 Genomic DNA Translation: CAA31683.1
M15545 Genomic DNA Translation: AAA25107.1
PIRiS02514

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Enzyme and pathway databases

UniPathwayiUPA00782

Family and domain databases

CDDicd00852 NifB, 1 hit
Gene3Di3.20.20.70, 1 hit
3.30.420.130, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR003731 Di-Nase_FeMo-co_biosynth
IPR036105 DiNase_FeMo-co_biosyn_sf
IPR006638 Elp3/MiaB/NifB
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR005980 Nase_CF_NifB
IPR034165 NifB_C
IPR007197 rSAM
PANTHERiPTHR43787:SF9 PTHR43787:SF9, 1 hit
PfamiView protein in Pfam
PF02579 Nitro_FeMo-Co, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
SUPFAMiSSF53146 SSF53146, 1 hit
TIGRFAMsiTIGR01290 nifB, 1 hit
PROSITEiView protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIFB_KLEPN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P10390
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 11, 2019
This is version 93 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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