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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei7Proton acceptorBy similarity1
Active sitei129Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-2095-MONOMER
UniPathwayiUPA00031; UER00009

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:hisA
Ordered Locus Names:STM2076
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001420491 – 2451-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST245

Proteomic databases

PaxDbiP10372
PRIDEiP10372

Interactioni

Protein-protein interaction databases

STRINGi99287.STM2076

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi12 – 16Combined sources5
Helixi17 – 19Combined sources3
Beta strandi23 – 26Combined sources4
Helixi31 – 40Combined sources10
Beta strandi46 – 50Combined sources5
Helixi51 – 55Combined sources5
Helixi57 – 59Combined sources3
Helixi62 – 70Combined sources9
Beta strandi72 – 74Combined sources3
Beta strandi76 – 81Combined sources6
Helixi85 – 93Combined sources9
Beta strandi97 – 101Combined sources5
Helixi103 – 107Combined sources5
Helixi109 – 119Combined sources11
Helixi121 – 123Combined sources3
Beta strandi124 – 127Combined sources4
Beta strandi130 – 132Combined sources3
Beta strandi138 – 142Combined sources5
Turni143 – 146Combined sources4
Beta strandi147 – 152Combined sources6
Helixi153 – 160Combined sources8
Helixi161 – 163Combined sources3
Beta strandi167 – 172Combined sources6
Turni173 – 177Combined sources5
Helixi184 – 193Combined sources10
Beta strandi197 – 203Combined sources7
Helixi208 – 212Combined sources5
Turni213 – 217Combined sources5
Beta strandi220 – 224Combined sources5
Helixi226 – 229Combined sources4
Helixi235 – 244Combined sources10

3D structure databases

ProteinModelPortaliP10372
SMRiP10372
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGiENOG4105CJV Bacteria
COG0106 LUCA
HOGENOMiHOG000224614
KOiK01814
OMAiEWLHLVD
PhylomeDBiP10372

Family and domain databases

CDDicd04732 HisA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01014 HisA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006062 His_biosynth
IPR006063 HisA
IPR023016 Isoase_HisA
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00977 His_biosynth, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00007 TIGR00007, 1 hit

Sequencei

Sequence statusi: Complete.

P10372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIPALDLID GTVVRLHQGD YARQRDYGND PLPRLQDYAA QGAGVLHLVD
60 70 80 90 100
LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRTEEDVAA LLKAGVARVV
110 120 130 140 150
IGSTAVKSPD VVKGWFERFG AQALVLALDV RIDEHGTKQV AVSGWQENSG
160 170 180 190 200
VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQIAFQ
210 220 230 240
SSGGIGDIDD IAALRGTGVR GVIVGRALLE GKFTVKEAIQ CWQNV
Length:245
Mass (Da):26,089
Last modified:January 23, 2002 - v2
Checksum:i20CF123F43DCE925
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123 – 124AL → GV in CAA31827 (PubMed:3062174).Curated2
Sequence conflicti135H → Q in CAA31827 (PubMed:3062174).Curated1
Sequence conflicti177G → A in CAA31827 (PubMed:3062174).Curated1
Sequence conflicti211I → DV (PubMed:3062174).Curated1
Sequence conflicti222V → M in CAA31827 (PubMed:3062174).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13464 Genomic DNA Translation: CAA31827.1
AE006468 Genomic DNA Translation: AAL20980.1
PIRiJS0161 ISEBIC
RefSeqiNP_461021.1, NC_003197.2
WP_000586409.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL20980; AAL20980; STM2076
GeneIDi1253597
KEGGistm:STM2076
PATRICifig|99287.12.peg.2198

Similar proteinsi

Entry informationi

Entry nameiHIS4_SALTY
AccessioniPrimary (citable) accession number: P10372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2002
Last modified: February 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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