UniProtKB - P10372 (HIS4_SALTY)
Protein
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Gene
hisA
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Catalytic activityi
- 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamideEC:5.3.1.16
: L-histidine biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG)
- Histidine biosynthesis bifunctional protein HisIE (hisI)
- Histidine biosynthesis bifunctional protein HisIE (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
- Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol-phosphate aminotransferase (hisC)
- Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 7 | Proton acceptorBy similarity | 1 | |
Active sitei | 129 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: GO_Central
GO - Biological processi
- histidine biosynthetic process Source: GO_Central
- tryptophan biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Isomerase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00009 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)Alternative name(s): Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase |
Gene namesi | Name:hisA Ordered Locus Names:STM2076 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142049 | 1 – 245 | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST | 245 |
Proteomic databases
PaxDbi | P10372 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10372 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the HisA/HisF family.Curated
Phylogenomic databases
HOGENOMi | CLU_048577_1_2_6 |
OMAi | EWLHLVD |
PhylomeDBi | P10372 |
Family and domain databases
CDDi | cd04732, HisA, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01014, HisA, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR006063, HisA IPR023016, Isoase_HisA-like IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00007, TIGR00007, 1 hit |
i Sequence
Sequence statusi: Complete.
P10372-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIPALDLID GTVVRLHQGD YARQRDYGND PLPRLQDYAA QGAGVLHLVD
60 70 80 90 100
LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRTEEDVAA LLKAGVARVV
110 120 130 140 150
IGSTAVKSPD VVKGWFERFG AQALVLALDV RIDEHGTKQV AVSGWQENSG
160 170 180 190 200
VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQIAFQ
210 220 230 240
SSGGIGDIDD IAALRGTGVR GVIVGRALLE GKFTVKEAIQ CWQNV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 123 – 124 | AL → GV in CAA31827 (PubMed:3062174).Curated | 2 | |
Sequence conflicti | 135 | H → Q in CAA31827 (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 177 | G → A in CAA31827 (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 211 | I → DV (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 222 | V → M in CAA31827 (PubMed:3062174).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13464 Genomic DNA Translation: CAA31827.1 AE006468 Genomic DNA Translation: AAL20980.1 |
PIRi | JS0161, ISEBIC |
RefSeqi | NP_461021.1, NC_003197.2 WP_000586409.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL20980; AAL20980; STM2076 |
GeneIDi | 1253597 |
KEGGi | stm:STM2076 |
PATRICi | fig|99287.12.peg.2198 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13464 Genomic DNA Translation: CAA31827.1 AE006468 Genomic DNA Translation: AAL20980.1 |
PIRi | JS0161, ISEBIC |
RefSeqi | NP_461021.1, NC_003197.2 WP_000586409.1, NC_003197.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5A5W | X-ray | 1.60 | A | 1-245 | [»] | |
5AB3 | X-ray | 1.80 | A/B/C | 1-245 | [»] | |
5ABT | X-ray | 1.65 | A | 1-245 | [»] | |
5AC6 | X-ray | 1.99 | A | 1-245 | [»] | |
5AC7 | X-ray | 1.90 | A/B | 1-245 | [»] | |
5AC8 | X-ray | 1.70 | A | 1-245 | [»] | |
5AHE | X-ray | 1.70 | A | 1-245 | [»] | |
5AHF | X-ray | 2.20 | A | 1-245 | [»] | |
5AHI | X-ray | 2.00 | A | 1-245 | [»] | |
5G1T | X-ray | 1.70 | A | 1-245 | [»] | |
5G1Y | X-ray | 1.80 | A | 1-15 | [»] | |
A | 17-245 | [»] | ||||
5G2H | X-ray | 1.90 | A | 1-245 | [»] | |
5G2I | X-ray | 1.60 | A | 1-15 | [»] | |
A | 17-245 | [»] | ||||
5G2W | X-ray | 2.10 | A | 1-245 | [»] | |
5G4E | X-ray | 2.65 | A/B | 1-15 | [»] | |
A/B | 17-245 | [»] | ||||
5G4W | X-ray | 2.30 | A | 1-245 | [»] | |
5G5I | X-ray | 2.00 | A | 1-245 | [»] | |
5L6U | X-ray | 1.60 | A | 1-15 | [»] | |
A | 17-245 | [»] | ||||
5L9F | X-ray | 2.59 | A/B | 1-245 | [»] | |
SMRi | P10372 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PaxDbi | P10372 |
Genome annotation databases
EnsemblBacteriai | AAL20980; AAL20980; STM2076 |
GeneIDi | 1253597 |
KEGGi | stm:STM2076 |
PATRICi | fig|99287.12.peg.2198 |
Phylogenomic databases
HOGENOMi | CLU_048577_1_2_6 |
OMAi | EWLHLVD |
PhylomeDBi | P10372 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00009 |
Family and domain databases
CDDi | cd04732, HisA, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01014, HisA, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR006063, HisA IPR023016, Isoase_HisA-like IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00007, TIGR00007, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS4_SALTY | |
Accessioni | P10372Primary (citable) accession number: P10372 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2002 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families