hisA

Functionⁱ

Catalytic activityⁱ

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayⁱ: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:

ATP phosphoribosyltransferase (hisG)
Histidine biosynthesis bifunctional protein HisIE (hisI)
Histidine biosynthesis bifunctional protein HisIE (hisI)
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
Histidine biosynthesis bifunctional protein HisB (hisB)
Histidinol-phosphate aminotransferase (hisC)
Histidine biosynthesis bifunctional protein HisB (hisB)
Histidinol dehydrogenase (hisD)

This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	7	Proton acceptorBy similarity		1
Active siteⁱ	129	Proton donorBy similarity		1

GO - Molecular functionⁱ

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 12356303

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to DNA damage stimulus
Source: EcoliWiki
Inferred from expression patternⁱ
- 11967071
histidine biosynthetic process
Source: EcoCyc
Inferred from direct assayⁱ
- 12356303
tryptophan biosynthetic process Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase
Biological process	Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PRIBFAICARPISOM-MONOMER MetaCyc:PRIBFAICARPISOM-MONOMER
UniPathwayⁱ	UPA00031; UER00009

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16) Alternative name(s): Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesⁱ	Name:hisA Ordered Locus Names:b2024, JW2006
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10444 hisA

EcoGeneⁱ

EG10444 hisA

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000142004	1 – 245	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST		245

Proteomic databases

EPDⁱ	P10371
PaxDbⁱ	P10371
PRIDEⁱ	P10371

2D gel databases

SWISS-2DPAGEⁱ	P10371

SWISS-2DPAGEⁱ

P10371

Interactionⁱ

Subunit structureⁱ

Monomer.

Protein-protein interaction databases

BioGridⁱ	4260420, 26 interactors
IntActⁱ	P10371, 3 interactors
STRINGⁱ	316385.ECDH10B_2172

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P10371
SMRⁱ	P10371
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105CJV Bacteria COG0106 LUCA
HOGENOMⁱ	HOG000224614
InParanoidⁱ	P10371
KEGG Orthology (KO) More...KOⁱ	K01814
OMAⁱ	EWLHLVD
PhylomeDBⁱ	P10371

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd04732 HisA, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_01014 HisA, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006062 His_biosynth IPR006063 HisA IPR023016 Isoase_HisA IPR011060 RibuloseP-bd_barrel
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00977 His_biosynth, 1 hit
SUPFAMⁱ	SSF51366 SSF51366, 1 hit
TIGRFAMsⁱ	TIGR00007 TIGR00007, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P10371-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIIPALDLID GTVVRLHQGD YGKQRDYGND PLPRLQDYAA QGAEVLHLVD 
        60         70         80         90        100
LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRSEEDVAA LLEAGVARVV 
       110        120        130        140        150
VGSTAVKSQD MVKGWFERFG ADALVLALDV RIDEQGNKQV AVSGWQENSG 
       160        170        180        190        200
VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQVAFQ 
       210        220        230        240 
SSGGIGDIDD VAALRGTGVR GVIVGRALLE GKFTVKEAIA CWQNA

Length:245

Mass (Da):26,033

Last modified:November 1, 1997 - v2

Checksum:ⁱB21D590E4214EE7E

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	84	S → T in CAA31816 (PubMed:3062174).Curated		1
Sequence conflictⁱ	126	L → V in CAA31816 (PubMed:3062174).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X13462 Genomic DNA Translation: CAA31816.1 U00096 Genomic DNA Translation: AAC75085.2 AP009048 Genomic DNA Translation: BAA15855.1
PIRⁱ	G64967 ISECIC
NCBI Reference Sequences More...RefSeqⁱ	NP_416528.2, NC_000913.3 WP_000586462.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75085; AAC75085; b2024 BAA15855; BAA15855; BAA15855
GeneIDⁱ	946521
KEGGⁱ	ecj:JW2006 eco:b2024
PATRICⁱ	fig\|1411691.4.peg.228

Protein	Similar proteins		Species	Score	Length
P10371	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO8A		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECOBW		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECODH		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECOSE		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECOLX		245
+8

Protein	Similar proteins		Species	Score	Length
P10371	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO57		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO5E		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECOLX		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO5C		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		Escherichia coli O157:H7 str. SS52		245
+667

Protein	Similar proteins		Species	Score	Length
P10371	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO57		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO5E		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECOLX		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		ECO5C		245
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase		Escherichia coli O157:H7 str. SS52		245
+2079

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X13462 Genomic DNA Translation: CAA31816.1 U00096 Genomic DNA Translation: AAC75085.2 AP009048 Genomic DNA Translation: BAA15855.1
PIRⁱ	G64967 ISECIC
NCBI Reference Sequences More...RefSeqⁱ	NP_416528.2, NC_000913.3 WP_000586462.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P10371
SMRⁱ	P10371
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260420, 26 interactors
IntActⁱ	P10371, 3 interactors
STRINGⁱ	316385.ECDH10B_2172

2D gel databases

SWISS-2DPAGEⁱ	P10371

SWISS-2DPAGEⁱ

P10371

Proteomic databases

EPDⁱ	P10371
PaxDbⁱ	P10371
PRIDEⁱ	P10371

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75085; AAC75085; b2024 BAA15855; BAA15855; BAA15855
GeneIDⁱ	946521
KEGGⁱ	ecj:JW2006 eco:b2024
PATRICⁱ	fig\|1411691.4.peg.228

Organism-specific databases

EchoBASEⁱ	EB0439
EcoGeneⁱ	EG10444 hisA

Phylogenomic databases

eggNOGⁱ	ENOG4105CJV Bacteria COG0106 LUCA
HOGENOMⁱ	HOG000224614
InParanoidⁱ	P10371
KEGG Orthology (KO) More...KOⁱ	K01814
OMAⁱ	EWLHLVD
PhylomeDBⁱ	P10371

Enzyme and pathway databases

UniPathwayⁱ	UPA00031; UER00009
BioCycⁱ	EcoCyc:PRIBFAICARPISOM-MONOMER MetaCyc:PRIBFAICARPISOM-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P10371

PROⁱ

PR:P10371

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd04732 HisA, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_01014 HisA, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006062 His_biosynth IPR006063 HisA IPR023016 Isoase_HisA IPR011060 RibuloseP-bd_barrel
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00977 His_biosynth, 1 hit
SUPFAMⁱ	SSF51366 SSF51366, 1 hit
TIGRFAMsⁱ	TIGR00007 TIGR00007, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HIS4_ECOLI
Accessionⁱ	P10371Primary (citable) accession number: P10371 Secondary accession number(s): P78078
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	November 1, 1997
	Last modified:	March 28, 2018
	This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P10371 (HIS4_ECOLI)

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1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-histidine biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: L-histidine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ