UniProtKB - P10371 (HIS4_ECOLI)
Protein
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Gene
hisA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamideEC:5.3.1.16
: L-histidine biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 7 | Proton acceptorBy similarity | 1 | |
Active sitei | 129 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- histidine biosynthetic process Source: EcoCyc
- tryptophan biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Isomerase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:PRIBFAICARPISOM-MONOMER MetaCyc:PRIBFAICARPISOM-MONOMER |
UniPathwayi | UPA00031;UER00009 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)Alternative name(s): Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase |
Gene namesi | Name:hisA Ordered Locus Names:b2024, JW2006 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142004 | 1 – 245 | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST | 245 |
Proteomic databases
jPOSTi | P10371 |
PaxDbi | P10371 |
PRIDEi | P10371 |
2D gel databases
SWISS-2DPAGEi | P10371 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4260420, 26 interactors |
IntActi | P10371, 3 interactors |
STRINGi | 511145.b2024 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HisA/HisF family.Curated
Phylogenomic databases
eggNOGi | COG0106, Bacteria |
HOGENOMi | CLU_048577_1_2_6 |
InParanoidi | P10371 |
PhylomeDBi | P10371 |
Family and domain databases
CDDi | cd04732, HisA, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01014, HisA, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR006063, HisA_bact_arch IPR044524, Isoase_HisA-like IPR023016, Isoase_HisA-like_bact IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR43090, PTHR43090, 1 hit |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00007, TIGR00007, 1 hit |
i Sequence
Sequence statusi: Complete.
P10371-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIPALDLID GTVVRLHQGD YGKQRDYGND PLPRLQDYAA QGAEVLHLVD
60 70 80 90 100
LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRSEEDVAA LLEAGVARVV
110 120 130 140 150
VGSTAVKSQD MVKGWFERFG ADALVLALDV RIDEQGNKQV AVSGWQENSG
160 170 180 190 200
VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQVAFQ
210 220 230 240
SSGGIGDIDD VAALRGTGVR GVIVGRALLE GKFTVKEAIA CWQNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | S → T in CAA31816 (PubMed:3062174).Curated | 1 | |
Sequence conflicti | 126 | L → V in CAA31816 (PubMed:3062174).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31816.1 U00096 Genomic DNA Translation: AAC75085.2 AP009048 Genomic DNA Translation: BAA15855.1 |
PIRi | G64967, ISECIC |
RefSeqi | NP_416528.2, NC_000913.3 WP_000586462.1, NZ_SSTT01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75085; AAC75085; b2024 BAA15855; BAA15855; BAA15855 |
GeneIDi | 946521 |
KEGGi | ecj:JW2006 eco:b2024 |
PATRICi | fig|1411691.4.peg.228 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31816.1 U00096 Genomic DNA Translation: AAC75085.2 AP009048 Genomic DNA Translation: BAA15855.1 |
PIRi | G64967, ISECIC |
RefSeqi | NP_416528.2, NC_000913.3 WP_000586462.1, NZ_SSTT01000011.1 |
3D structure databases
SMRi | P10371 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260420, 26 interactors |
IntActi | P10371, 3 interactors |
STRINGi | 511145.b2024 |
2D gel databases
SWISS-2DPAGEi | P10371 |
Proteomic databases
jPOSTi | P10371 |
PaxDbi | P10371 |
PRIDEi | P10371 |
Genome annotation databases
EnsemblBacteriai | AAC75085; AAC75085; b2024 BAA15855; BAA15855; BAA15855 |
GeneIDi | 946521 |
KEGGi | ecj:JW2006 eco:b2024 |
PATRICi | fig|1411691.4.peg.228 |
Organism-specific databases
EchoBASEi | EB0439 |
Phylogenomic databases
eggNOGi | COG0106, Bacteria |
HOGENOMi | CLU_048577_1_2_6 |
InParanoidi | P10371 |
PhylomeDBi | P10371 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00009 |
BioCyci | EcoCyc:PRIBFAICARPISOM-MONOMER MetaCyc:PRIBFAICARPISOM-MONOMER |
Miscellaneous databases
PROi | PR:P10371 |
Family and domain databases
CDDi | cd04732, HisA, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01014, HisA, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR006063, HisA_bact_arch IPR044524, Isoase_HisA-like IPR023016, Isoase_HisA-like_bact IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR43090, PTHR43090, 1 hit |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00007, TIGR00007, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS4_ECOLI | |
Accessioni | P10371Primary (citable) accession number: P10371 Secondary accession number(s): P78078 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families