UniProtKB - P10351 (XDH_DROME)
Protein
Xanthine dehydrogenase
Gene
ry
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid (By similarity).By similarity1 Publication
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
- FADBy similarity
- Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | Iron-sulfur 1By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1By similarity | 1 | |
Metal bindingi | 51 | Iron-sulfur 1By similarity | 1 | |
Metal bindingi | 73 | Iron-sulfur 1By similarity | 1 | |
Metal bindingi | 113 | Iron-sulfur 2By similarity | 1 | |
Metal bindingi | 116 | Iron-sulfur 2By similarity | 1 | |
Metal bindingi | 148 | Iron-sulfur 2By similarity | 1 | |
Metal bindingi | 150 | Iron-sulfur 2By similarity | 1 | |
Binding sitei | 335 | FADBy similarity | 1 | |
Binding sitei | 358 | FADBy similarity | 1 | |
Binding sitei | 406 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 424 | FADBy similarity | 1 | |
Metal bindingi | 772 | MolybdenumBy similarity | 1 | |
Metal bindingi | 803 | Molybdenum; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 807 | SubstrateBy similarity | 1 | |
Binding sitei | 885 | SubstrateBy similarity | 1 | |
Metal bindingi | 917 | Molybdenum; via amide nitrogenBy similarity | 1 | |
Binding sitei | 919 | SubstrateBy similarity | 1 | |
Metal bindingi | 1084 | Molybdenum; via amide nitrogenBy similarity | 1 | |
Active sitei | 1267 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 255 – 262 | FADBy similarity | 8 | |
Nucleotide bindingi | 345 – 349 | FADBy similarity | 5 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB
- electron transfer activity Source: InterPro
- FAD binding Source: InterPro
- flavin adenine dinucleotide binding Source: UniProtKB
- iron ion binding Source: InterPro
- molybdopterin cofactor binding Source: UniProtKB
- xanthine dehydrogenase activity Source: FlyBase
- xanthine oxidase activity Source: InterPro
GO - Biological processi
- arginine metabolic process Source: FlyBase
- compound eye pigmentation Source: FlyBase
- determination of adult lifespan Source: FlyBase
- glycerophospholipid metabolic process Source: FlyBase
- purine nucleobase metabolic process Source: FlyBase
- pyrimidine nucleobase metabolic process Source: FlyBase
- tryptophan metabolic process Source: FlyBase
- xanthine catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD |
Enzyme and pathway databases
Reactomei | R-DME-74259, Purine catabolism R-DME-964975, Vitamins B6 activation to pyridoxal phosphate |
Names & Taxonomyi
Protein namesi | Recommended name: Xanthine dehydrogenase (EC:1.17.1.4)Short name: XD Alternative name(s): Protein rosy locus |
Gene namesi | Name:ry Synonyms:XDH ORF Names:CG7642 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003308, ry |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Cytosol
- cytosol Source: GO_Central
Peroxisome
- peroxisome Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166079 | 1 – 1335 | Xanthine dehydrogenaseAdd BLAST | 1335 |
Proteomic databases
PaxDbi | P10351 |
PRIDEi | P10351 |
Expressioni
Gene expression databases
Bgeei | FBgn0003308, Expressed in adult Malpighian tubule (Drosophila) and 49 other tissues |
Genevisiblei | P10351, DM |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 66692, 7 interactors |
IntActi | P10351, 4 interactors |
STRINGi | 7227.FBpp0082172 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 91 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 227 – 416 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 190 |
Sequence similaritiesi
Belongs to the xanthine dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0430, Eukaryota |
GeneTreei | ENSGT00950000183114 |
HOGENOMi | CLU_001681_1_2_1 |
InParanoidi | P10351 |
OMAi | VPLMYKQ |
OrthoDBi | 48717at2759 |
PhylomeDBi | P10351 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR000674, Ald_Oxase/Xan_DH_a/b IPR036856, Ald_Oxase/Xan_DH_a/b_sf IPR016208, Ald_Oxase/xanthine_DH IPR008274, AldOxase/xan_DH_Mopterin-bd IPR037165, AldOxase/xan_DH_Mopterin-bd_sf IPR005107, CO_DH_flav_C IPR036683, CO_DH_flav_C_dom_sf IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR002346, Mopterin_DH_FAD-bd IPR022407, OxRdtase_Mopterin_BS IPR014307, Xanthine_DH_ssu |
Pfami | View protein in Pfam PF01315, Ald_Xan_dh_C, 1 hit PF02738, Ald_Xan_dh_C2, 1 hit PF03450, CO_deh_flav_C, 1 hit PF00941, FAD_binding_5, 1 hit PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
PIRSFi | PIRSF000127, Xanthine_DH, 1 hit |
SMARTi | View protein in SMART SM01008, Ald_Xan_dh_C, 1 hit SM01092, CO_deh_flav_C, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit SSF54665, SSF54665, 1 hit SSF55447, SSF55447, 1 hit SSF56003, SSF56003, 1 hit SSF56176, SSF56176, 1 hit |
TIGRFAMsi | TIGR02963, xanthine_xdhA, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51387, FAD_PCMH, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
i Sequence
Sequence statusi: Complete.
P10351-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNSVLVFFV NGKKVTEVSP DPECTLLTFL REKLRLCGTK LGCAEGGCGA
60 70 80 90 100
CTVMVSRLDR RANKIRHLAV NACLTPVCSM HGCAVTTVEG IGSTKTRLHP
110 120 130 140 150
VQERLAKAHG SQCGFCTPGI VMSMYALLRN AEQPSMRDLE VAFQGNLCRC
160 170 180 190 200
TGYRPILEGY KTFTKEFACG MGEKCCKVSG KGCGTDAETD DKLFERSEFQ
210 220 230 240 250
PLDPSQEPIF PPELQLSDAF DSQSLIFSSD RVTWYRPTNL EELLQLKAKH
260 270 280 290 300
PAAKLVVGNT EVGVEVKFKH FLYPHLINPT QVKELLEIKE NQDGIYFGAA
310 320 330 340 350
VSLMEIDALL RQRIEQLPES ETRLFQCTVD MLHYFAGKQI RNVACLGGNI
360 370 380 390 400
MTGSPISDMN PVLSAAGAQL EVASFVDGKL QKRSVHMGTG FFTGYRRNVI
410 420 430 440 450
EAHEVLLGIH FRKTTPDQYI VAFKQARRRD DDIAIVNAAI NVRFEEKSNI
460 470 480 490 500
VAEISMAFGG MAPTTVLAPR TSQLMVGQEW SHQLVERVAE SLCTELPLAA
510 520 530 540 550
SAPGGMIAYR RALVVSLFFK AYLAISLKLS KSGITSSDAL PSEERSGAET
560 570 580 590 600
FHTPVLKSAQ LFERVCSDQP ICDPIGRPKV HAAALKQATG EAIYTDDIPR
610 620 630 640 650
MDGEVYLAFV LSTKPRAKIT KLDASEALAL DGVHQFFCYK DLTEHENEVG
660 670 680 690 700
PVFHDEHVFA AGEVHCYGQI VGAIAADNKA LAQRAARLVK VEYEELSPVI
710 720 730 740 750
VTIEQAIEHK SYFPDYPRFV TKGNVEEALA QADHTFEGTC RMGGQEHFYL
760 770 780 790 800
ETHAALAVPR DSDELELFCS TQHPSEVQKL VAHVTALPAH RVVCRAKRLG
810 820 830 840 850
GGFGGKESRG ISVALPVALA AYRMGRPVRC MLDRDEDMLI TGTRHPFLFK
860 870 880 890 900
YKVGFTKEGL ITACDIECYN NAGWSMDLSF SVLERAMFHF ENCYRIPNVR
910 920 930 940 950
VGGWVCKTNL PSNTAFRGFG GPQGMYAGEH IIRDVARIVG RDVVDVMRLN
960 970 980 990 1000
FYKTGDYTHY HQQLEHFPIE RCLEDCLKQS RYDEKRQEIA RFNRENRWRK
1010 1020 1030 1040 1050
RGMAVVPTKY GIAFGVMHLN QAGSLINIYG DGSVLLSHGG VEIGQGLNTK
1060 1070 1080 1090 1100
MIQCAARALG IPSELIHISE TATDKVPNTS PTAASVGSDL NGMAVLDACE
1110 1120 1130 1140 1150
KLNKRLAPIK EALPGGTWKE WINKAYFDRV SLSATGFYAM PGIGYHPETN
1160 1170 1180 1190 1200
PNARTYSYYT NGVGVTVVEI DCLTGDHQVL STDIVMDIGS SLNPAIDIGQ
1210 1220 1230 1240 1250
IEGAFMQGYG LFTLEELMYS PQGMLYSRGP GMYKLPGFAD IPGEFNVSLL
1260 1270 1280 1290 1300
TGAPNPRAVY SSKAVGEPPL FIGSSAFFAI KEAIAAARED QGLSGDFPLE
1310 1320 1330
APSTSARIRI ACQDKFTELL EIPEPGSFTP WNIVP
Sequence cautioni
The sequence AAM11042 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 | A → P in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 106 | A → P no nucleotide entry (PubMed:3036645).Curated | 1 | |
Sequence conflicti | 316 | Q → L in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 316 | Q → L no nucleotide entry (PubMed:3036646).Curated | 1 | |
Sequence conflicti | 542 | S → P in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 542 | S → P no nucleotide entry (PubMed:3036646).Curated | 1 | |
Sequence conflicti | 709 | H → L in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 709 | H → L no nucleotide entry (PubMed:3036646).Curated | 1 | |
Sequence conflicti | 730 | A → S in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 730 | A → S no nucleotide entry (PubMed:3036646).Curated | 1 | |
Sequence conflicti | 988 | E → D in CAA68409 (Ref. 1) Curated | 1 | |
Sequence conflicti | 988 | E → D no nucleotide entry (PubMed:3036646).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00308 Genomic DNA Translation: CAA68409.1 AE014297 Genomic DNA Translation: AAF54895.1 BT015293 mRNA Translation: AAT94522.1 AY094689 mRNA Translation: AAM11042.1 Different initiation. |
PIRi | S07245 |
RefSeqi | NP_524337.1, NM_079613.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0082704; FBpp0082172; FBgn0003308 |
GeneIDi | 41605 |
KEGGi | dme:Dmel_CG7642 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00308 Genomic DNA Translation: CAA68409.1 AE014297 Genomic DNA Translation: AAF54895.1 BT015293 mRNA Translation: AAT94522.1 AY094689 mRNA Translation: AAM11042.1 Different initiation. |
PIRi | S07245 |
RefSeqi | NP_524337.1, NM_079613.3 |
3D structure databases
SMRi | P10351 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66692, 7 interactors |
IntActi | P10351, 4 interactors |
STRINGi | 7227.FBpp0082172 |
Proteomic databases
PaxDbi | P10351 |
PRIDEi | P10351 |
Genome annotation databases
EnsemblMetazoai | FBtr0082704; FBpp0082172; FBgn0003308 |
GeneIDi | 41605 |
KEGGi | dme:Dmel_CG7642 |
Organism-specific databases
CTDi | 41605 |
FlyBasei | FBgn0003308, ry |
Phylogenomic databases
eggNOGi | KOG0430, Eukaryota |
GeneTreei | ENSGT00950000183114 |
HOGENOMi | CLU_001681_1_2_1 |
InParanoidi | P10351 |
OMAi | VPLMYKQ |
OrthoDBi | 48717at2759 |
PhylomeDBi | P10351 |
Enzyme and pathway databases
Reactomei | R-DME-74259, Purine catabolism R-DME-964975, Vitamins B6 activation to pyridoxal phosphate |
Miscellaneous databases
BioGRID-ORCSi | 41605, 0 hits in 3 CRISPR screens |
GenomeRNAii | 41605 |
PROi | PR:P10351 |
Gene expression databases
Bgeei | FBgn0003308, Expressed in adult Malpighian tubule (Drosophila) and 49 other tissues |
Genevisiblei | P10351, DM |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR000674, Ald_Oxase/Xan_DH_a/b IPR036856, Ald_Oxase/Xan_DH_a/b_sf IPR016208, Ald_Oxase/xanthine_DH IPR008274, AldOxase/xan_DH_Mopterin-bd IPR037165, AldOxase/xan_DH_Mopterin-bd_sf IPR005107, CO_DH_flav_C IPR036683, CO_DH_flav_C_dom_sf IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR002346, Mopterin_DH_FAD-bd IPR022407, OxRdtase_Mopterin_BS IPR014307, Xanthine_DH_ssu |
Pfami | View protein in Pfam PF01315, Ald_Xan_dh_C, 1 hit PF02738, Ald_Xan_dh_C2, 1 hit PF03450, CO_deh_flav_C, 1 hit PF00941, FAD_binding_5, 1 hit PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
PIRSFi | PIRSF000127, Xanthine_DH, 1 hit |
SMARTi | View protein in SMART SM01008, Ald_Xan_dh_C, 1 hit SM01092, CO_deh_flav_C, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit SSF54665, SSF54665, 1 hit SSF55447, SSF55447, 1 hit SSF56003, SSF56003, 1 hit SSF56176, SSF56176, 1 hit |
TIGRFAMsi | TIGR02963, xanthine_xdhA, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51387, FAD_PCMH, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XDH_DROME | |
Accessioni | P10351Primary (citable) accession number: P10351 Secondary accession number(s): Q6AWF5, Q8SXC4, Q9VFZ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | June 20, 2003 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families