UniProtKB - P10297 (RIP1_PHYAM)
Protein
Antiviral protein I
Gene
PAP1
Organism
Phytolacca americana (American pokeweed) (Phytolacca decandra)
Status
Functioni
Possesses antiviral potency. Inhibits viral infection of plants (tobacco mosaic virus) (PubMed:10403789). Inhibits protein synthesis (PubMed:2930487, PubMed:6091760, PubMed:2248976, PubMed:10403789, PubMed:10595542). Releases both adenine and guanine from Escherichia coli rRNA in vitro. Activity on guanine is 20 times slower than that on adenine (PubMed:10595542).5 Publications
Miscellaneous
Depurinates genomic RNA of immunodeficiency virus type-I (HIV-I) and bacteriophage (MS 2) RNA. Inhibits the replication of HIV-1 in human peripheral blood mononuclear cells with IC50 value of 14 nM.1 Publication
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.3 Publications EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 94 | By similarity | 1 | |
Binding sitei | 95 | Substrate; via carbonyl oxygen and amide nitrogenCombined sources3 Publications | 1 | |
Binding sitei | 143 | Substrate; via carbonyl oxygenCombined sources3 Publications | 1 | |
Active sitei | 145 | By similarity | 1 | |
Binding sitei | 197 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 198 | By similarity | 1 | |
Active sitei | 201 | By similarity | 1 | |
Binding sitei | 201 | SubstrateCombined sources3 Publications | 1 |
GO - Molecular functioni
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protein synthesis inhibitor, Toxin |
Biological process | Antiviral defense, Plant defense |
Enzyme and pathway databases
BRENDAi | 3.2.2.22, 4806 |
Names & Taxonomyi
Protein namesi | Recommended name: Antiviral protein I (EC:3.2.2.223 Publications)Alternative name(s): PAP-C1 Publication PAP-I1 Publication PAP-R1 Publication Ribosome-inactivating protein rRNA N-glycosidase |
Gene namesi | Name:PAP1 |
Organismi | Phytolacca americana (American pokeweed) (Phytolacca decandra) |
Taxonomic identifieri | 3527 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Caryophyllales › Phytolaccaceae › Phytolacca |
Pathology & Biotechi
Toxic dosei
LD50 is 1.89 mg/kg by intraperitoneal injection into mice (at 48 hours post-injection).1 Publication
LD50 is 0.95 mg/kg by intraperitoneal injection into mice (at 14 days post-injection).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 5 PublicationsAdd BLAST | 22 | |
ChainiPRO_0000030781 | 23 – 285 | Antiviral protein IAdd BLAST | 263 | |
PropeptideiPRO_0000030782 | 286 – 313 | Add BLAST | 28 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 281 | Combined sources1 Publication | ||
Disulfide bondi | 107 ↔ 128 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Monomer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10297 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P10297 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10297-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSMLVVTIS IWLILAPTST WAVNTIIYNV GSTTISKYAT FLNDLRNEAK
60 70 80 90 100
DPSLKCYGIP MLPNTNTNPK YVLVELQGSN KKTITLMLRR NNLYVMGYSD
110 120 130 140 150
PFETNKCRYH IFNDISGTER QDVETTLCPN ANSRVSKNIN FDSRYPTLES
160 170 180 190 200
KAGVKSRSQV QLGIQILDSN IGKISGVMSF TEKTEAEFLL VAIQMVSEAA
210 220 230 240 250
RFKYIENQVK TNFNRAFNPN PKVLNLQETW GKISTAIHDA KNGVLPKPLE
260 270 280 290 300
LVDASGAKWI VLRVDEIKPD VALLNYVGGS CQTTYNQNAM FPQLIMSTYY
310
NYMVNLGDLF EGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55383 mRNA Translation: CAA39054.1 |
PIRi | S17757 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55383 mRNA Translation: CAA39054.1 |
PIRi | S17757 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D6A | X-ray | 2.10 | A/B | 23-284 | [»] | |
1PAF | X-ray | 2.50 | A/B | 23-284 | [»] | |
1PAG | X-ray | 2.80 | A/B | 23-284 | [»] | |
1QCG | X-ray | 2.10 | A/B | 23-284 | [»] | |
1QCI | X-ray | 2.00 | A/B | 23-284 | [»] | |
1QCJ | X-ray | 2.10 | A/B | 23-284 | [»] | |
SMRi | P10297 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 3.2.2.22, 4806 |
Miscellaneous databases
EvolutionaryTracei | P10297 |
Family and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIP1_PHYAM | |
Accessioni | P10297Primary (citable) accession number: P10297 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 1, 1993 | |
Last modified: | April 22, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families