Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - P10220 (LTP_HHV11)

Protein

Large tegument protein deneddylase

Gene

UL36

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.UniRule annotation9 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei52Important for catalytic activityUniRule annotation1
Active sitei65UniRule annotation1 Publication1
Active sitei197UniRule annotation1
Active sitei199UniRule annotation1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • modulation by virus of host protein ubiquitination Source: UniProtKB-UniRule
  • nuclear capsid assembly Source: AgBase
  • viral DNA genome replication Source: InterPro
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processHost-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Protein family/group databases

MEROPSiC76.001

Names & Taxonomyi

Protein namesi
Recommended name:
Large tegument protein deneddylaseUniRule annotation (EC:3.4.19.12UniRule annotation1 Publication, EC:3.4.22.-UniRule annotation1 Publication)
Gene namesi
ORF Names:UL36
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiViruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus ›
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome
  • UP000180652 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: AgBase
  • host cell nucleus Source: UniProtKB-SubCell
  • viral tegument Source: CACAO
View the complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65C → A: Complete loss of deubiquitination activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001160351 – 3164Large tegument protein deneddylaseAdd BLAST3164

Post-translational modificationi

Proteolytically processed, possibly into several polypeptides. Enzymatic activity is only detectable following cleavage of the UL36 protein, which occurs late during viral replication.1 Publication

Proteomic databases

PRIDEiP10220

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with inner tegument protein. Interacts with capsid vertex specific component CVC2. Interacts with the major capsid protein/MCP (By similarity). Interacts with VP16; this interaction is important for outer tegument association to the capsid. May form homodimers.UniRule annotationBy similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UL37P102212EBI-7694334,EBI-6880600

Protein-protein interaction databases

BioGridi971402, 4 interactors
IntActiP10220, 3 interactors
MINTiP10220

Structurei

Secondary structure

13164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP10220
SMRiP10220
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 263Peptidase C76UniRule annotationAdd BLAST219
Repeati2911 – 291212
Repeati2913 – 291422
Repeati2915 – 291632
Repeati2917 – 291842
Repeati2919 – 292052
Repeati2921 – 292262
Repeati2923 – 292472
Repeati2925 – 292682
Repeati2927 – 292892
Repeati2929 – 2930102
Repeati2931 – 2932112
Repeati2933 – 2934122
Repeati2935 – 2936132
Repeati2937 – 2938142
Repeati2939 – 2940152
Repeati2941 – 2942162
Repeati2943 – 2944172
Repeati2945 – 2946182
Repeati2947 – 2948192
Repeati2949 – 2950202
Repeati2951 – 2952212
Repeati2953 – 2954222
Repeati2955 – 2956232
Repeati2957 – 2958242
Repeati2959 – 2960252
Repeati2961 – 2962262
Repeati2963 – 2964272
Repeati2965 – 2966282
Repeati2967 – 2968292
Repeati2969 – 2970302
Repeati2971 – 2972312
Repeati2973 – 2974322
Repeati2975 – 2976332
Repeati2977 – 2978342
Repeati2979 – 2980352

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 273Deubiquitination activityUniRule annotationAdd BLAST273
Regioni426 – 432Nuclear localization signal2 Publications7
Regioni579 – 609Interaction with inner tegument proteinUniRule annotationAdd BLAST31
Regioni579 – 609Interaction with UL371 PublicationAdd BLAST31
Regioni2911 – 298035 X 2 AA tandem repeats of P-QAdd BLAST70

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi307 – 494Pro-richAdd BLAST188
Compositional biasi1161 – 1288Ala-richAdd BLAST128

Sequence similaritiesi

Belongs to the herpesviridae large tegument protein family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK21075
OrthoDBiVOG09000001

Family and domain databases

HAMAPiMF_04044 HSV_LTP, 1 hit
InterProiView protein in InterPro
IPR005210 Herpes_LT_deneddylase
IPR006928 Herpes_teg_USP
IPR034702 HSV_LTP
IPR038765 Papain_like_cys_pep_sf
PfamiView protein in Pfam
PF04843 Herpes_teg_N, 1 hit
PF03586 Herpes_UL36, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS51521 HTUSP, 1 hit

Sequencei

Sequence statusi: Complete.

P10220-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGGGNNTNPG GPVHKQAGSL ASRAHMIAGT PPHSTMERGG DRDIVVTGAR 
        60         70         80         90        100
NQFAPDLEPG GSVSCMRSSL SFLSLIFDVG PRDVLSAEAI EGCLVEGGEW 
       110        120        130        140        150
TRATAGPGPP RMCSIVELPN FLEYPGARGG LRCVFSRVYG EVGFFGEPAA 
       160        170        180        190        200
GLLETQCPAH TFFAGPWALR PLSYTLLTIG PLGMGLFRDG DTAYLFDPHG 
       210        220        230        240        250
LPEGTPAFIA KVRAGDMYPY LTYYTRDRPD VRWAGAMVFF VPSGPEPAAP 
       260        270        280        290        300
ADLTAAALHL YGASETYLQD EAFSERRVAI THPLRGEIAG LGEPCVGVGP 
       310        320        330        340        350
REGVGGPGPH PPTAAQSPPP TRARRDDRAS ETSRGTAGPS AKPEAKRPNR 
       360        370        380        390        400
APDDVWAVAL KGTPPTDPPS ADPPSADPPS AIPPPPPSAP KTPAAEAAEE 
       410        420        430        440        450
DDDDMRVLEM GVVPVGRHRA RYSAGLPKRR RPTWTPPSSV EDLTSGEKTK 
       460        470        480        490        500
RSAPPAKTKK KSTPKGKTPV GAAVPASVPE PVLASAPPDP AGPPVAEAGE 
       510        520        530        540        550
DDGPTVPASS QALEALKTRR SPEPPGADLA QLFEAHPNVA ATAVKFTACS 
       560        570        580        590        600
AALAREVAAC SRLTISALRS PYPASPGLLE LCVIFFFERV LAFLIENGAR 
       610        620        630        640        650
THTQAGVAGP AAALLEFTLN MLPWKTAVGD FLASTRLSLA DVAAHLPLVQ 
       660        670        680        690        700
HVLDENSLIG RLALAKLILV ARDVIRETDA FYGELADLEL QLRAAPPANL 
       710        720        730        740        750
YTRLGEWLLE RSQAHPDTLF APATPTHPEP LLYRVQALAK FARGEEIRVE 
       760        770        780        790        800
AEDRQMREAL DALARGVDAV SQHAGPLGVM PAPAGAAPQG APRPPPLGPE 
       810        820        830        840        850
AVQVRLEEVR TQARRAIEGA VKEYFYRGAV YSAKALQASD NNDRRFHVAS 
       860        870        880        890        900
AAVVPVVQLL ESLPVFDQHT RDIAQRAAIP APPPIATSPT AILLRDLIQR 
       910        920        930        940        950
GQTLDAPEDL AAWLSVLTDA ANQGLIERKP LDELARSIRD INDQQARRSS 
       960        970        980        990       1000
GLAELRRFDA LDAALGQQLD SDAAFVPAPG ASPYPDDGGL SPEATRMAEE 
      1010       1020       1030       1040       1050
ALRQARAMDA AKLTAELAPD ARARLRERAR SLEAMLEGAR ERAKVARDAR 
      1060       1070       1080       1090       1100
EKFLHKLQGV LRPLPDFVGL KACPAVLATL RASLPAGWSD LPEAVRGAPP 
      1110       1120       1130       1140       1150
EVTAALRADM WGLLGQYRDA LEHPTPDTAT ALSGLHPSFV VVLKNLFADA 
      1160       1170       1180       1190       1200
PETPFLLQFF ADHAPIIAHA VSNAINAGSA AVATADPAST VDAAVRAHRV 
      1210       1220       1230       1240       1250
LVDAVTALGA AASDPASPLA FLAAMADSAA GYVKATRLAL DARVAIAQLT 
      1260       1270       1280       1290       1300
TLGSAAADLV VQVRRAANQP EGEHASLIQA ATRATTGARE SLAGHEGRFG 
      1310       1320       1330       1340       1350
GLLHAEGTAG DHSPSGRALQ ELGKVIGATR RRADELEAAT ADLREKMAAQ 
      1360       1370       1380       1390       1400
RARSSHERWA ADVEAVLDRV ESGAEFDVVE LRRLQALAGT HGYNPRDFRK 
      1410       1420       1430       1440       1450
RAEQALGTNA KAVTLALETA LAFNPYTPEN QRHPMLPPLA AIHRIDWSAA 
      1460       1470       1480       1490       1500
FGAAADTYAD MFRVDTEPLA RLLRLAGGLL ERAQANDGFI DYHEAVLHLS 
      1510       1520       1530       1540       1550
EDLGGVPALR QYVPFFQKGY AEYVDIRDRL DALRADARRA IGSVALDLAA 
      1560       1570       1580       1590       1600
AAEEISAVRN DPAAAAELVR AGVTLPCPSE DALVACVAAL ERVDQSPVKD 
      1610       1620       1630       1640       1650
TAYAHYVAFV TRQDLADTKD AVVRAKQQRA EATERVTAGL REVLAARERR 
      1660       1670       1680       1690       1700
AQLEAEGLAN LKTLLKVVAV PATVAKTLDQ ARSAEEIADQ VEILVDQTEK 
      1710       1720       1730       1740       1750
ARELDVQAVA WLEHAQRTFE THPLSAASGD GPGLLTRQGA RLQALFDTRR 
      1760       1770       1780       1790       1800
RVEALRRSLE EAEAEWDEVW GRFGRVRGGA WKSPEGFRAA CEQLRALQDT 
      1810       1820       1830       1840       1850
TNTVSGLRAQ RDYERLPAKY QGVLGAKSAE RAGAVEELGG RVAQHADLSA 
      1860       1870       1880       1890       1900
RLRDEVVPRV AWEMNFDTLG GLLAEFDAVA GDLAPWAVEE FRGARELIQR 
      1910       1920       1930       1940       1950
RMGLYSAYAK ATGQTGAGAA AAPAPLLVDL RALDARARAS APPGQEADPQ 
      1960       1970       1980       1990       2000
MLRRRGEAYL RVSGGPGPLV LREATSTLDR PFAPSFLVPD GTPLQYALCF 
      2010       2020       2030       2040       2050
PAVTDKLGAL LMCPEAACIR PPLPTDTLES ASTVTAMYVL TVINRLQLAL 
      2060       2070       2080       2090       2100
SDAQAANFQL FGRFVRHRQA RWGASMDAAA ELYVALVATT LTREFGCRWA 
      2110       2120       2130       2140       2150
QLEWGGDAAA PGPPLGPQSS TRHRVSFNEN DVLVALVASS PEHIYTFWRL 
      2160       2170       2180       2190       2200
DLVRQHEYMH LTLPRAFQNA ADSMLFVQRL TPHPDARIRV LPAFSAGGPP 
      2210       2220       2230       2240       2250
TRGLMFGTRL ADWRRGKLSE TDPLAPWRSV PELGTERGAA LGKLSPAQAL 
      2260       2270       2280       2290       2300
AAVSVLGRMC LPSTALVALW TCMFPDDYTE YDSFDALLTA RLESGQTLSP 
      2310       2320       2330       2340       2350
SGGREASPPA PPNALYRPTG QHVAVPAAAT HRTPAARVTA MDLVLAAVLL 
      2360       2370       2380       2390       2400
GAPVVVALRN TTAFSRESEL ELCLTLFDSR ARGPDAALRD AVSSDIETWA 
      2410       2420       2430       2440       2450
VRLLHADLNP IENACLAAQL PRLSALIAER PLARGPPCLV LVDISMTPVA 
      2460       2470       2480       2490       2500
VLWENPDPPG PPDVRFVGSE ATEELPFVAG GEDVLAASAT DEDPFLARAI 
      2510       2520       2530       2540       2550
LGRPFDASLL SGELFPGHPV YQRAPDDQSP SVPNPTPGPV DLVGAEGSLG 
      2560       2570       2580       2590       2600
PGSLAPTLFT DATPGEPVPP RMWAWIHGLE ELASDDSGGP APLLAPDPLS 
      2610       2620       2630       2640       2650
PTADQSVPTS QCAPRPPGPA VTAREARPGV PAESTRPAPV GPRDDFRRLP 
      2660       2670       2680       2690       2700
SPQSSPAPPD ATAPRPPASS RASAASSSGS RARRHRRARS LARATQASAT 
      2710       2720       2730       2740       2750
TQGWRPPALP DTVAPVTDFA RPPAPPKPPE PAPHALVSGV PLPLGPQAAG 
      2760       2770       2780       2790       2800
QASPALPIDP VPPPVATGTV LPGGENRRPP LTSGPAPTPP RVPVGGPQRR 
      2810       2820       2830       2840       2850
LTRPAVASLS ESRESLPSPW DPADPTAPVL GRNPAEPTSS SPAGPSPPPP 
      2860       2870       2880       2890       2900
AVQPVAPPPT SGPPPTYLTL EGGVAPGGPV SRRPTTRQPV ATPTTSARPR 
      2910       2920       2930       2940       2950
GHLTVSRLSA PQPQPQPQPQ PQPQPQPQPQ PQPQPQPQPQ PQPQPQPQPQ 
      2960       2970       2980       2990       3000
PQPQPQPQPQ PQPQPQPQPQ PQPQPQPQPQ NGHVAPGEYP AVRFRAPQNR 
      3010       3020       3030       3040       3050
PSVPASASST NPRTGSSLSG VSSWASSLAL HIDATPPPVS LLQTLYVSDD 
      3060       3070       3080       3090       3100
EDSDATSLFL SDSEAEALDP LPGEPHSPIT NEPFSALSAD DSQEVTRLQF 
      3110       3120       3130       3140       3150
GPPPVSANAV LSRRYVQRTG RSALAVLIRA CYRLQQQLQR TRRALLHHSD 
      3160 
AVLTSLHHVR MLLG
Length:3,164
Mass (Da):335,862
Last modified:July 1, 1989 - v1
Checksum:iCC5D31FF4F9FE3F4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti234A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti307P → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti375 – 379Missing in strain: Nonneuroinvasive mutant HF10. 5
Natural varianti392T → P in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti620N → S in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti624W → R in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti671A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti885I → M in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1023A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1244V → G in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1373G → S in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1389G → D in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1419T → M in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1470A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1604A → S in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1605H → D in strain: 17 syn+ and Nonneuroinvasive mutant HF10. 1
Natural varianti1642E → D in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1695V → L in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1729G → S in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1777R → C in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1888V → M in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti1973E → K in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2267V → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2540V → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2545A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2646F → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2666P → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2748A → S in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2856A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2875A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2894T → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti2973 – 2978Missing in strain: Nonneuroinvasive mutant HF10. 6
Natural varianti3095V → A in strain: Nonneuroinvasive mutant HF10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32311.1
DQ889502 Genomic DNA Translation: ABI63498.1
FJ593289 Genomic DNA Translation: ACM62259.1
PIRiI30085 WMBEH6
RefSeqiYP_009137111.1, NC_001806.2

Genome annotation databases

GeneIDi2703357
KEGGivg:2703357

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32311.1
DQ889502 Genomic DNA Translation: ABI63498.1
FJ593289 Genomic DNA Translation: ACM62259.1
PIRiI30085 WMBEH6
RefSeqiYP_009137111.1, NC_001806.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TT0X-ray2.60A/B1625-1757[»]
4TT1X-ray2.75A/B1625-1757[»]
ProteinModelPortaliP10220
SMRiP10220
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971402, 4 interactors
IntActiP10220, 3 interactors
MINTiP10220

Protein family/group databases

MEROPSiC76.001

Proteomic databases

PRIDEiP10220

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703357
KEGGivg:2703357

Phylogenomic databases

KOiK21075
OrthoDBiVOG09000001

Family and domain databases

HAMAPiMF_04044 HSV_LTP, 1 hit
InterProiView protein in InterPro
IPR005210 Herpes_LT_deneddylase
IPR006928 Herpes_teg_USP
IPR034702 HSV_LTP
IPR038765 Papain_like_cys_pep_sf
PfamiView protein in Pfam
PF04843 Herpes_teg_N, 1 hit
PF03586 Herpes_UL36, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS51521 HTUSP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLTP_HHV11
AccessioniPrimary (citable) accession number: P10220
Secondary accession number(s): B9VQG4, Q09I97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 10, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again