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Protein

DNA helicase/primase complex-associated protein

Gene

UL8

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The primase-associated factor has no known catalytic activity in the complex and may serve to facilitate the formation of the replisome by directly interacting with the origin-binding protein and the polymerase.UniRule annotation2 Publications

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
  • DNA replication Source: UniProtKB-KW

Keywordsi

Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase/primase complex-associated proteinUniRule annotation
Short name:
HEPAUniRule annotation
Alternative name(s):
Primase-associated factorUniRule annotation
Gene namesi
ORF Names:UL8
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome
  • UP000180652 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001158561 – 750DNA helicase/primase complex-associated proteinAdd BLAST750

Proteomic databases

PRIDEiP10192

Interactioni

Subunit structurei

Associates with the primase and the helicase to form the helicase-primase complex. Interacts with the origin-binding protein. Interacts with the polymerase catalytic subunit.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UL30P042934EBI-7185538,EBI-8615017

Protein-protein interaction databases

BioGridi971456, 1 interactor
DIPiDIP-1097N
IntActiP10192, 2 interactors
MINTiP10192

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 343Poly-Ala7

Sequence similaritiesi

Belongs to the herpesviridae HEPA family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900001Z

Family and domain databases

HAMAPiMF_04010 HSV_HEPA, 1 hit
InterProiView protein in InterPro
IPR004996 HSV_HEPA
PfamiView protein in Pfam
PF03324 Herpes_HEPA, 1 hit

Sequencei

Sequence statusi: Complete.

P10192-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF
60 70 80 90 100
AEVSVTATEL RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA
110 120 130 140 150
LYACVLAALE RQTGPVALFA PLRIGSDPRT GLVVKVERAS WGPPAAPRAA
160 170 180 190 200
LLVAEANIDI DPMALAARVA EHPDARLAWA RLAAIRDTPQ CASAASLTVN
210 220 230 240 250
ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR GHPQRVTARV
260 270 280 290 300
LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF
310 320 330 340 350
EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA
360 370 380 390 400
VGARVVLPPR AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV
410 420 430 440 450
GPVWTARFCF PGLRAQLLAA LADLGGSGLA DPHGRTGLAR LDALVVAAPS
460 470 480 490 500
EPWAGAVLER LVPDTCNACP ALRQLLGGVM AAVCLQIEET ASSVKFAVCG
510 520 530 540 550
GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR ANAVRLRHPL
560 570 580 590 600
CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD
610 620 630 640 650
TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV
660 670 680 690 700
SPFEASDPLP PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP
710 720 730 740 750
LVLPPVDCAH HLREILREIE LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA
Length:750
Mass (Da):79,926
Last modified:July 1, 1989 - v1
Checksum:iECA9ABD0E85CB392
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti247T → M in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti330A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti353A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti467N → D in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti471A → V in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti543 – 544AV → GL in strain: Nonneuroinvasive mutant HF10 and 17 syn+. 2
Natural varianti612C → G in strain: Nonneuroinvasive mutant HF10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32344.1
M19120 Genomic DNA Translation: AAA45823.1
DQ889502 Genomic DNA Translation: ABI63470.1
FJ593289 Genomic DNA Translation: ACM62230.1
PIRiC29890 WMBEX8
RefSeqiYP_009137082.1, NC_001806.2

Genome annotation databases

GeneIDi2703432
KEGGivg:2703432

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32344.1
M19120 Genomic DNA Translation: AAA45823.1
DQ889502 Genomic DNA Translation: ABI63470.1
FJ593289 Genomic DNA Translation: ACM62230.1
PIRiC29890 WMBEX8
RefSeqiYP_009137082.1, NC_001806.2

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971456, 1 interactor
DIPiDIP-1097N
IntActiP10192, 2 interactors
MINTiP10192

Proteomic databases

PRIDEiP10192

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703432
KEGGivg:2703432

Phylogenomic databases

OrthoDBiVOG0900001Z

Family and domain databases

HAMAPiMF_04010 HSV_HEPA, 1 hit
InterProiView protein in InterPro
IPR004996 HSV_HEPA
PfamiView protein in Pfam
PF03324 Herpes_HEPA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHEPA_HHV11
AccessioniPrimary (citable) accession number: P10192
Secondary accession number(s): B9VQD5, Q09IC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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