UniProtKB - P10181 (ROUGH_DROME)
Protein
Homeobox protein rough
Gene
ro
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required to establish the unique cell identity of photoreceptors R2 and R5 and consequently for ommatidial assembly in the developing eye imaginal disk. Repression of expression in R8 photoreceptor by senseless (sens) is an essential mechanism of R8 cell fate determination.3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 191 – 250 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- establishment of ommatidial planar polarity Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- R2/R5 cell differentiation Source: FlyBase
- R2/R5 cell fate commitment Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to stimulus Source: UniProtKB-KW
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Sensory transduction, Transcription, Transcription regulation, Vision |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein rough |
Gene namesi | Name:ro ORF Names:CG6348 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003267, ro |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Note: Restricted to nuclei in the developing retina.
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
R2 and R5 fail to be correctly determined and appear to be transformed into cells of the R3/4/1/6 subtype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049076 | 1 – 350 | Homeobox protein roughAdd BLAST | 350 |
Proteomic databases
PaxDbi | P10181 |
PRIDEi | P10181 |
Expressioni
Tissue specificityi
Expressed in cells within, and posterior to, the morphogenetic furrow of the eye imaginal disk, expression is rapidly restricted to the R2, R3, R4, and R5 precursor cells.2 Publications
Gene expression databases
Bgeei | FBgn0003267, Expressed in eye disc (Drosophila) and 17 other tissues |
Genevisiblei | P10181, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 68125, 18 interactors |
IntActi | P10181, 8 interactors |
STRINGi | 7227.FBpp0084461 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 120 – 131 | Gln/His-richAdd BLAST | 12 | |
Compositional biasi | 322 – 328 | Gln-rich | 7 |
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0489, Eukaryota |
GeneTreei | ENSGT00940000172642 |
HOGENOMi | CLU_792903_0_0_1 |
InParanoidi | P10181 |
OMAi | PHFSAGF |
OrthoDBi | 1583482at2759 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P10181-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQRHKVEIGS PDGSPGIKRS DSLDPIANTT ILSVPQRPSS PRQFFERLYG
60 70 80 90 100
HLETRSSENG EIDVGTHAHK PPPCDTPYHS DGGSVSSPDI SISDERTSLA
110 120 130 140 150
AYPAYDFYGH AKDYPQHPSQ QHQQHHHHHH HPPQLVHQKL SYVSPPPAIA
160 170 180 190 200
AGGAANPVLP HAFPAGFPSD PHFSAGFSAF LARRRRKEGR QRRQRTTFST
210 220 230 240 250
EQTLRLEVEF HRNEYISRSR RFELAETLRL TETQIKIWFQ NRRAKDKRIE
260 270 280 290 300
KAQIDQHYRN FVVANGFMSS IMGQAATTMP PGGVTGGVAV GVGLNYYAAA
310 320 330 340 350
ATPAALPKDN TQDANFIDID DQFQRQQQQK QQQQQQQQRR RETTTPINIC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 304 | A → T in AAA56800 (PubMed:2903798).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23629 Genomic DNA Translation: AAA56800.1 AE014297 Genomic DNA Translation: AAF56635.1 AE014297 Genomic DNA Translation: AAN14093.1 BT022142 mRNA Translation: AAY51537.1 X12528 Genomic DNA Translation: CAC32035.1 |
PIRi | A30046 S03168 |
RefSeqi | NP_524521.1, NM_079797.3 NP_733173.1, NM_170294.1 |
Genome annotation databases
EnsemblMetazoai | FBtr0085090; FBpp0084460; FBgn0003267 FBtr0085091; FBpp0084461; FBgn0003267 |
GeneIDi | 43234 |
KEGGi | dme:Dmel_CG6348 |
UCSCi | CG6348-RA, d. melanogaster CG6348-RB, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23629 Genomic DNA Translation: AAA56800.1 AE014297 Genomic DNA Translation: AAF56635.1 AE014297 Genomic DNA Translation: AAN14093.1 BT022142 mRNA Translation: AAY51537.1 X12528 Genomic DNA Translation: CAC32035.1 |
PIRi | A30046 S03168 |
RefSeqi | NP_524521.1, NM_079797.3 NP_733173.1, NM_170294.1 |
3D structure databases
SMRi | P10181 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68125, 18 interactors |
IntActi | P10181, 8 interactors |
STRINGi | 7227.FBpp0084461 |
Proteomic databases
PaxDbi | P10181 |
PRIDEi | P10181 |
Genome annotation databases
EnsemblMetazoai | FBtr0085090; FBpp0084460; FBgn0003267 FBtr0085091; FBpp0084461; FBgn0003267 |
GeneIDi | 43234 |
KEGGi | dme:Dmel_CG6348 |
UCSCi | CG6348-RA, d. melanogaster CG6348-RB, d. melanogaster |
Organism-specific databases
CTDi | 19875 |
FlyBasei | FBgn0003267, ro |
Phylogenomic databases
eggNOGi | KOG0489, Eukaryota |
GeneTreei | ENSGT00940000172642 |
HOGENOMi | CLU_792903_0_0_1 |
InParanoidi | P10181 |
OMAi | PHFSAGF |
OrthoDBi | 1583482at2759 |
Miscellaneous databases
BioGRID-ORCSi | 43234, 0 hits in 1 CRISPR screen |
GenomeRNAii | 43234 |
PROi | PR:P10181 |
Gene expression databases
Bgeei | FBgn0003267, Expressed in eye disc (Drosophila) and 17 other tissues |
Genevisiblei | P10181, DM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ROUGH_DROME | |
Accessioni | P10181Primary (citable) accession number: P10181 Secondary accession number(s): Q24487, Q4V714, Q9VBA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase