UniProtKB - P10151 (LEUO_ECOLI)
Protein
HTH-type transcriptional regulator LeuO
Gene
leuO
Organism
Escherichia coli (strain K12)
Status
Functioni
A global transcription factor. Activates transcription of the 9 following operons; yjjQ-bglJ, yjjP, acrEF, ybdO, yjcRQP, casABCDE12, rhsD-ybbC, fepE and gltF, in most cases it probably interferes with silencing by H-NS and activates transcription. Represses transcription of the 3 following operons; uxaCA, sdaCB and btsT. H-NS repression of the bgl operon, leading to the ability to metabolize some beta-glucosides. It also directly activates the bgl operon. Activation is H-NS and BglJ-RcsB independent.4 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 39 – 58 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: InterPro
- sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00519 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator LeuO |
Gene namesi | Name:leuO Ordered Locus Names:b0076, JW0075 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Has no effect on leucine biosynthesis nor on bgl operon silencing.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105660 | 1 – 314 | HTH-type transcriptional regulator LeuOAdd BLAST | 314 |
Proteomic databases
PaxDbi | P10151 |
PRIDEi | P10151 |
Expressioni
Inductioni
Gene expression is repressed by H-NS, activated by itself. More highly expressed in minimal than rich medium, poorly expressed in exoponential growth, levels increase in stationary phase (at protein level).1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259484, 4 interactors 853278, 3 interactors |
IntActi | P10151, 5 interactors |
STRINGi | 511145.b0076 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P10151 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 79 | HTH lysR-typePROSITE-ProRule annotationAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the LysR transcriptional regulatory family.Curated
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_39_0_6 |
InParanoidi | P10151 |
PhylomeDBi | P10151 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
i Sequence
Sequence statusi: Complete.
P10151-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ
60 70 80 90 100
PAVSNAVARL KVMFNDELFV RYGRGIQPTA RAFQLFGSVR QALQLVQNEL
110 120 130 140 150
PGSGFEPASS ERVFHLCVCS PLDSILTSQI YNHIEQIAPN IHVMFKSSLN
160 170 180 190 200
QNTEHQLRYQ ETEFVISYED FHRPEFTSVP LFKDEMVLVA SKNHPTIKGP
210 220 230 240 250
LLKHDVYNEQ HAAVSLDRFA SFSQPWYDTV DKQASIAYQG MAMMSVLSVV
260 270 280 290 300
SQTHLVAIAP RWLAEEFAES LELQVLPLPL KQNSRTCYLS WHEAAGRDKG
310
HQWMEEQLVS ICKR
Sequence cautioni
The sequence AAA83880 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA38853 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 291 – 314 | Missing in AAA85299 (PubMed:3413113).CuratedAdd BLAST | 24 | |
Sequence conflicti | 291 – 314 | Missing in CAA38853 (PubMed:1630901).CuratedAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21150 Genomic DNA Translation: AAA85299.1 X55034 Genomic DNA Translation: CAA38853.1 Different initiation. U00096 Genomic DNA Translation: AAC73187.2 AP009048 Genomic DNA Translation: BAB96645.2 M12891 Genomic DNA Translation: AAA83880.1 Different initiation. |
PIRi | D64729 |
RefSeqi | NP_414618.4, NC_000913.3 WP_001115472.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73187; AAC73187; b0076 BAB96645; BAB96645; BAB96645 |
GeneIDi | 949034 |
KEGGi | ecj:JW0075 eco:b0076 |
PATRICi | fig|1411691.4.peg.2205 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21150 Genomic DNA Translation: AAA85299.1 X55034 Genomic DNA Translation: CAA38853.1 Different initiation. U00096 Genomic DNA Translation: AAC73187.2 AP009048 Genomic DNA Translation: BAB96645.2 M12891 Genomic DNA Translation: AAA83880.1 Different initiation. |
PIRi | D64729 |
RefSeqi | NP_414618.4, NC_000913.3 WP_001115472.1, NZ_STEB01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6GZ0 | X-ray | 1.52 | A | 109-314 | [»] | |
6GZ1 | X-ray | 1.74 | A | 109-314 | [»] | |
6GZ2 | X-ray | 1.94 | A | 109-314 | [»] | |
SMRi | P10151 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259484, 4 interactors 853278, 3 interactors |
IntActi | P10151, 5 interactors |
STRINGi | 511145.b0076 |
Proteomic databases
PaxDbi | P10151 |
PRIDEi | P10151 |
Genome annotation databases
EnsemblBacteriai | AAC73187; AAC73187; b0076 BAB96645; BAB96645; BAB96645 |
GeneIDi | 949034 |
KEGGi | ecj:JW0075 eco:b0076 |
PATRICi | fig|1411691.4.peg.2205 |
Organism-specific databases
EchoBASEi | EB0526 |
Phylogenomic databases
eggNOGi | COG0583, Bacteria |
HOGENOMi | CLU_039613_39_0_6 |
InParanoidi | P10151 |
PhylomeDBi | P10151 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00519 |
Miscellaneous databases
PROi | PR:P10151 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR005119, LysR_subst-bd IPR000847, Tscrpt_reg_HTH_LysR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00126, HTH_1, 1 hit PF03466, LysR_substrate, 1 hit |
PRINTSi | PR00039, HTHLYSR |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50931, HTH_LYSR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEUO_ECOLI | |
Accessioni | P10151Primary (citable) accession number: P10151 Secondary accession number(s): P75640 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families