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Protein

HTH-type transcriptional regulator LeuO

Gene

leuO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A global transcription factor. Activates transcription of the 9 following operons; yjjQ-bglJ, yjjP, acrEF, ybdO, yjcRQP, casABCDE12, rhsD-ybbC, fepE and gltF, in most cases it probably interferes with silencing by H-NS and activates transcription. Represses transcription of the 3 following operons; uxaCA, sdaCB and btsT. H-NS repression of the bgl operon, leading to the ability to metabolize some beta-glucosides. It also directly activates the bgl operon. Activation is H-NS and BglJ-RcsB independent.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi39 – 58H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding transcription factor activity Source: InterPro
  • sequence-specific DNA binding Source: CollecTF

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00519

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator LeuO
Gene namesi
Name:leuO
Ordered Locus Names:b0076, JW0075
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10531 leuO

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

Pathology & Biotechi

Disruption phenotypei

Has no effect on leucine biosynthesis nor on bgl operon silencing.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056601 – 314HTH-type transcriptional regulator LeuOAdd BLAST314

Proteomic databases

PaxDbiP10151
PRIDEiP10151

Expressioni

Inductioni

Gene expression is repressed by H-NS, activated by itself. More highly expressed in minimal than rich medium, poorly expressed in exoponential growth, levels increase in stationary phase (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259484, 4 interactors
IntActiP10151, 5 interactors
STRINGi316407.85674318

Structurei

3D structure databases

ProteinModelPortaliP10151
SMRiP10151
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 79HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Phylogenomic databases

eggNOGiENOG41064T2 Bacteria
ENOG4110BBF LUCA
HOGENOMiHOG000116777
InParanoidiP10151
KOiK05798
OMAiICSPLDI
PhylomeDBiP10151

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR005119 LysR_subst-bd
IPR000847 Tscrpt_reg_HTH_LysR
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00126 HTH_1, 1 hit
PF03466 LysR_substrate, 1 hit
PRINTSiPR00039 HTHLYSR
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50931 HTH_LYSR, 1 hit

Sequencei

Sequence statusi: Complete.

P10151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEVQTDHPE TAELSKPQLR MVDLNLLTVF DAVMQEQNIT RAAHVLGMSQ
60 70 80 90 100
PAVSNAVARL KVMFNDELFV RYGRGIQPTA RAFQLFGSVR QALQLVQNEL
110 120 130 140 150
PGSGFEPASS ERVFHLCVCS PLDSILTSQI YNHIEQIAPN IHVMFKSSLN
160 170 180 190 200
QNTEHQLRYQ ETEFVISYED FHRPEFTSVP LFKDEMVLVA SKNHPTIKGP
210 220 230 240 250
LLKHDVYNEQ HAAVSLDRFA SFSQPWYDTV DKQASIAYQG MAMMSVLSVV
260 270 280 290 300
SQTHLVAIAP RWLAEEFAES LELQVLPLPL KQNSRTCYLS WHEAAGRDKG
310
HQWMEEQLVS ICKR
Length:314
Mass (Da):35,695
Last modified:November 1, 1997 - v3
Checksum:i25605FA08C1073E3
GO

Sequence cautioni

The sequence AAA83880 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA38853 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291 – 314Missing in AAA85299 (PubMed:3413113).CuratedAdd BLAST24
Sequence conflicti291 – 314Missing in CAA38853 (PubMed:1630901).CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21150 Genomic DNA Translation: AAA85299.1
X55034 Genomic DNA Translation: CAA38853.1 Different initiation.
U00096 Genomic DNA Translation: AAC73187.2
AP009048 Genomic DNA Translation: BAB96645.2
M12891 Genomic DNA Translation: AAA83880.1 Different initiation.
PIRiD64729
RefSeqiNP_414618.4, NC_000913.3
WP_001115472.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73187; AAC73187; b0076
BAB96645; BAB96645; BAB96645
GeneIDi949034
KEGGiecj:JW0075
eco:b0076
PATRICifig|1411691.4.peg.2205

Similar proteinsi

Entry informationi

Entry nameiLEUO_ECOLI
AccessioniPrimary (citable) accession number: P10151
Secondary accession number(s): P75640
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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