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UniProtKB - P10149 (STXA_BPH30)
Protein
Shiga-like toxin 1 subunit A
Gene
stxA
Organism
Bacteriophage H30
Status
Functioni
The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 189 | By similarity | 1 |
GO - Molecular functioni
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- modulation of host virulence by virus Source: UniProtKB-KW
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protein synthesis inhibitor, Toxin, Viral exotoxin |
Biological process | Modulation of host virulence by virus, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Shiga-like toxin 1 subunit A (EC:3.2.2.22)Short name: SLT-1 A subunit Short name: SLT-1a Short name: SLT-Ia Alternative name(s): Verocytotoxin 1 subunit A Verotoxin 1 subunit A rRNA N-glycosidase 1 |
Gene namesi | Name:stxA |
Organismi | Bacteriophage H30 |
Taxonomic identifieri | 12371 [NCBI] |
Taxonomic lineagei | Viruses › unclassified bacterial viruses |
Virus hosti | Escherichia coli [TaxID: 562] |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Add BLAST | 22 | |
ChainiPRO_0000030790 | 23 – 315 | Shiga-like toxin 1 subunit AAdd BLAST | 293 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 264 ↔ 283 | By similarity |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 273 – 274 | Cleavage; by furinBy similarity | 2 |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Shiga-like toxin contains a single subunit A and five copies of subunit B.
Protein-protein interaction databases
DIPi | DIP-6144N |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 273 | A1By similarityAdd BLAST | 251 | |
Regioni | 274 – 315 | A2By similarityAdd BLAST | 42 |
Sequence similaritiesi
Belongs to the ribosome-inactivating protein family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P10149-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIIIFRVLT FFFVIFSVNV VAKEFTLDFS TAKTYVDSLN VIRSAIGTPL
60 70 80 90 100
QTISSGGTSL LMIDSGTGDN LFAVDVRGID PEEGRFNNLR LIVERNNLYV
110 120 130 140 150
TGFVNRTNNV FYRFADFSHV TFPGTTAVTL SGDSSYTTLQ RVAGISRTGM
160 170 180 190 200
QINRHSLTTS YLDLMSHSGT SLTQSVARAM LRFVTVTAEA LRFRQIQRGF
210 220 230 240 250
RTTLDDLSGR SYVMTAEDVD LTLNWGRLSS VLPDYHGQDS VRVGRISFGS
260 270 280 290 300
INAILGSVAL ILNCHHHASR VARMASDEFP SMCPADGRVR GITHNKILWD
310
SSTLGAILMR RTISS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23980 Genomic DNA Translation: AAA72732.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23980 Genomic DNA Translation: AAA72732.1 |
3D structure databases
SMRi | P10149 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-6144N |
Family and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 IPR016331, Shiga-like_toxin_subunit_A |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PIRSFi | PIRSF001924, Shigella_toxin_subunit_A, 1 hit |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STXA_BPH30 | |
Accessioni | P10149Primary (citable) accession number: P10149 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 1, 1989 | |
Last modified: | September 29, 2021 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |