Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alcohol dehydrogenase 4

Gene

ADH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces acetaldehyde to ethanol during glucose fermentation. Specific for ethanol. Shows drastically reduced activity towards primary alcohols from 4 carbon atoms upward. Isomers of aliphatic alcohol, as well as secondary alcohols and glycerol are not used at all.1 Publication

Miscellaneous

While ADH4 is expressed at only low levels in laboratory strains, it is often highly expressed in brewing strains.
Present with 125 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+1 Publication, Fe2+1 PublicationNote: Zinc. May bind iron when zinc levels are limiting.1 Publication

Activity regulationi

Inhibited by EDTA.1 Publication

Kineticsi

  1. KM=59 mM for NAD1 Publication
  2. KM=122 µM for NADH1 Publication
  3. KM=2.83 mM for acetaldehyde1 Publication
  4. KM=16.7 mM for ethanol1 Publication

    pH dependencei

    Optimum pH is 8.3.1 Publication

    GO - Molecular functioni

    • alcohol dehydrogenase (NAD) activity Source: SGD
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
    • fermentation Source: SGD

    Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:YGL256W-MONOMER
    YEAST:YGL256W-MONOMER
    ReactomeiR-SCE-880009 Interconversion of 2-oxoglutarate and 2-hydroxyglutarate
    SABIO-RKiP10127

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alcohol dehydrogenase 4 (EC:1.1.1.1)
    Alternative name(s):
    Alcohol dehydrogenase IV
    Short name:
    ADHIV
    Gene namesi
    Name:ADH4
    Synonyms:ZRG5
    Ordered Locus Names:YGL256W
    ORF Names:NRC465
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGL256W
    SGDiS000003225 ADH4

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000878171 – 382Alcohol dehydrogenase 4Add BLAST382

    Proteomic databases

    MaxQBiP10127
    PaxDbiP10127
    PRIDEiP10127

    Expressioni

    Inductioni

    Induced by transcription factor ZAP1 in response to zinc deficiency.5 Publications

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi33023, 207 interactors
    DIPiDIP-4812N
    IntActiP10127, 10 interactors
    MINTiP10127
    STRINGi4932.YGL256W

    Structurei

    3D structure databases

    ProteinModelPortaliP10127
    SMRiP10127
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000003849
    HOGENOMiHOG000243333
    InParanoidiP10127
    KOiK13954
    OMAiMNGFDKG
    OrthoDBiEOG092C28AS

    Family and domain databases

    InterProiView protein in InterPro
    IPR001670 ADH_Fe/GldA
    IPR018211 ADH_Fe_CS
    PfamiView protein in Pfam
    PF00465 Fe-ADH, 1 hit
    PROSITEiView protein in PROSITE
    PS00913 ADH_IRON_1, 1 hit
    PS00060 ADH_IRON_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P10127-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSSVTGFYIP PISFFGEGAL EETADYIKNK DYKKALIVTD PGIAAIGLSG
    60 70 80 90 100
    RVQKMLEERD LNVAIYDKTQ PNPNIANVTA GLKVLKEQNS EIVVSIGGGS
    110 120 130 140 150
    AHDNAKAIAL LATNGGEIGD YEGVNQSKKA ALPLFAINTT AGTASEMTRF
    160 170 180 190 200
    TIISNEEKKI KMAIIDNNVT PAVAVNDPST MFGLPPALTA ATGLDALTHC
    210 220 230 240 250
    IEAYVSTASN PITDACALKG IDLINESLVA AYKDGKDKKA RTDMCYAEYL
    260 270 280 290 300
    AGMAFNNASL GYVHALAHQL GGFYHLPHGV CNAVLLPHVQ EANMQCPKAK
    310 320 330 340 350
    KRLGEIALHF GASQEDPEET IKALHVLNRT MNIPRNLKEL GVKTEDFEIL
    360 370 380
    AEHAMHDACH LTNPVQFTKE QVVAIIKKAY EY
    Length:382
    Mass (Da):41,142
    Last modified:July 22, 2008 - v3
    Checksum:i7DFFD43830FB269B
    GO

    Sequence cautioni

    The sequence CAA64131 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA96976 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti60D → G in CAA29410 (PubMed:2823079).Curated1
    Sequence conflicti88Q → E in CAA29410 (PubMed:2823079).Curated1
    Sequence conflicti310F → C in CAA29410 (PubMed:2823079).Curated1
    Sequence conflicti339E → D in CAA29410 (PubMed:2823079).Curated1
    Sequence conflicti348E → D in CAA29410 (PubMed:2823079).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X05992 Genomic DNA Translation: CAA29410.1
    X94357 Genomic DNA Translation: CAA64131.1 Different initiation.
    Z72778 Genomic DNA Translation: CAA96976.1 Different initiation.
    BK006941 Genomic DNA Translation: DAA07863.1
    PIRiS61605 DEBY4
    RefSeqiNP_011258.2, NM_001181122.1

    Genome annotation databases

    EnsemblFungiiYGL256W; YGL256W; YGL256W
    GeneIDi852636
    KEGGisce:YGL256W

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X05992 Genomic DNA Translation: CAA29410.1
    X94357 Genomic DNA Translation: CAA64131.1 Different initiation.
    Z72778 Genomic DNA Translation: CAA96976.1 Different initiation.
    BK006941 Genomic DNA Translation: DAA07863.1
    PIRiS61605 DEBY4
    RefSeqiNP_011258.2, NM_001181122.1

    3D structure databases

    ProteinModelPortaliP10127
    SMRiP10127
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33023, 207 interactors
    DIPiDIP-4812N
    IntActiP10127, 10 interactors
    MINTiP10127
    STRINGi4932.YGL256W

    Proteomic databases

    MaxQBiP10127
    PaxDbiP10127
    PRIDEiP10127

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYGL256W; YGL256W; YGL256W
    GeneIDi852636
    KEGGisce:YGL256W

    Organism-specific databases

    EuPathDBiFungiDB:YGL256W
    SGDiS000003225 ADH4

    Phylogenomic databases

    GeneTreeiENSGT00390000003849
    HOGENOMiHOG000243333
    InParanoidiP10127
    KOiK13954
    OMAiMNGFDKG
    OrthoDBiEOG092C28AS

    Enzyme and pathway databases

    BioCyciMetaCyc:YGL256W-MONOMER
    YEAST:YGL256W-MONOMER
    ReactomeiR-SCE-880009 Interconversion of 2-oxoglutarate and 2-hydroxyglutarate
    SABIO-RKiP10127

    Miscellaneous databases

    PROiPR:P10127

    Family and domain databases

    InterProiView protein in InterPro
    IPR001670 ADH_Fe/GldA
    IPR018211 ADH_Fe_CS
    PfamiView protein in Pfam
    PF00465 Fe-ADH, 1 hit
    PROSITEiView protein in PROSITE
    PS00913 ADH_IRON_1, 1 hit
    PS00060 ADH_IRON_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiADH4_YEAST
    AccessioniPrimary (citable) accession number: P10127
    Secondary accession number(s): D6VV79
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 22, 2008
    Last modified: September 12, 2018
    This is version 159 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again