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UniProtKB - P10127 (ADH4_YEAST)
Protein
Alcohol dehydrogenase 4
Gene
ADH4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Reduces acetaldehyde to ethanol during glucose fermentation. Specific for ethanol. Shows drastically reduced activity towards primary alcohols from 4 carbon atoms upward. Isomers of aliphatic alcohol, as well as secondary alcohols and glycerol are not used at all.
2 PublicationsMiscellaneous
While ADH4 is expressed at only low levels in laboratory strains, it is often highly expressed in brewing strains.1 Publication
Present with 125 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.1.1.11 Publication
- EC:1.1.1.11 PublicationThis reaction proceeds in the backward1 Publication direction.
Cofactori
Zn2+1 Publication, Fe2+1 PublicationNote: Zinc. May bind iron when zinc levels are limiting.1 Publication
Activity regulationi
Inhibited by EDTA.1 Publication
Kineticsi
- KM=59 mM for NAD1 Publication
- KM=122 µM for NADH1 Publication
- KM=2.83 mM for acetaldehyde1 Publication
- KM=16.7 mM for ethanol1 Publication
pH dependencei
Optimum pH is 8.3.1 Publication
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
- fermentation Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
SABIO-RKi | P10127 |
Names & Taxonomyi
Protein namesi | Recommended name: Alcohol dehydrogenase 4 (EC:1.1.1.11 Publication)Alternative name(s): Alcohol dehydrogenase IV Short name: ADHIV |
Gene namesi | Name:ADH4 Synonyms:ZRG5 Ordered Locus Names:YGL256W ORF Names:NRC465 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003225, ADH4 |
VEuPathDBi | FungiDB:YGL256W |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087817 | 1 – 382 | Alcohol dehydrogenase 4Add BLAST | 382 |
Proteomic databases
MaxQBi | P10127 |
PaxDbi | P10127 |
PRIDEi | P10127 |
Expressioni
Inductioni
Induced by transcription factor ZAP1 in response to zinc deficiency.5 Publications
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33023, 209 interactors |
DIPi | DIP-4812N |
IntActi | P10127, 10 interactors |
MINTi | P10127 |
STRINGi | 4932.YGL256W |
Miscellaneous databases
RNActi | P10127, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_0_1 |
InParanoidi | P10127 |
OMAi | HAMSHQV |
Family and domain databases
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR018211, ADH_Fe_CS IPR039697, Alcohol_dehydrogenase_Fe |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
PROSITEi | View protein in PROSITE PS00913, ADH_IRON_1, 1 hit PS00060, ADH_IRON_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P10127-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSVTGFYIP PISFFGEGAL EETADYIKNK DYKKALIVTD PGIAAIGLSG
60 70 80 90 100
RVQKMLEERD LNVAIYDKTQ PNPNIANVTA GLKVLKEQNS EIVVSIGGGS
110 120 130 140 150
AHDNAKAIAL LATNGGEIGD YEGVNQSKKA ALPLFAINTT AGTASEMTRF
160 170 180 190 200
TIISNEEKKI KMAIIDNNVT PAVAVNDPST MFGLPPALTA ATGLDALTHC
210 220 230 240 250
IEAYVSTASN PITDACALKG IDLINESLVA AYKDGKDKKA RTDMCYAEYL
260 270 280 290 300
AGMAFNNASL GYVHALAHQL GGFYHLPHGV CNAVLLPHVQ EANMQCPKAK
310 320 330 340 350
KRLGEIALHF GASQEDPEET IKALHVLNRT MNIPRNLKEL GVKTEDFEIL
360 370 380
AEHAMHDACH LTNPVQFTKE QVVAIIKKAY EY
Sequence cautioni
The sequence CAA64131 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA96976 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | D → G in CAA29410 (PubMed:2823079).Curated | 1 | |
Sequence conflicti | 88 | Q → E in CAA29410 (PubMed:2823079).Curated | 1 | |
Sequence conflicti | 310 | F → C in CAA29410 (PubMed:2823079).Curated | 1 | |
Sequence conflicti | 339 | E → D in CAA29410 (PubMed:2823079).Curated | 1 | |
Sequence conflicti | 348 | E → D in CAA29410 (PubMed:2823079).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05992 Genomic DNA Translation: CAA29410.1 X94357 Genomic DNA Translation: CAA64131.1 Different initiation. Z72778 Genomic DNA Translation: CAA96976.1 Different initiation. BK006941 Genomic DNA Translation: DAA07863.1 |
PIRi | S61605, DEBY4 |
RefSeqi | NP_011258.2, NM_001181122.1 |
Genome annotation databases
EnsemblFungii | YGL256W_mRNA; YGL256W; YGL256W |
GeneIDi | 852636 |
KEGGi | sce:YGL256W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05992 Genomic DNA Translation: CAA29410.1 X94357 Genomic DNA Translation: CAA64131.1 Different initiation. Z72778 Genomic DNA Translation: CAA96976.1 Different initiation. BK006941 Genomic DNA Translation: DAA07863.1 |
PIRi | S61605, DEBY4 |
RefSeqi | NP_011258.2, NM_001181122.1 |
3D structure databases
AlphaFoldDBi | P10127 |
SMRi | P10127 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33023, 209 interactors |
DIPi | DIP-4812N |
IntActi | P10127, 10 interactors |
MINTi | P10127 |
STRINGi | 4932.YGL256W |
Proteomic databases
MaxQBi | P10127 |
PaxDbi | P10127 |
PRIDEi | P10127 |
Genome annotation databases
EnsemblFungii | YGL256W_mRNA; YGL256W; YGL256W |
GeneIDi | 852636 |
KEGGi | sce:YGL256W |
Organism-specific databases
SGDi | S000003225, ADH4 |
VEuPathDBi | FungiDB:YGL256W |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_0_1 |
InParanoidi | P10127 |
OMAi | HAMSHQV |
Enzyme and pathway databases
SABIO-RKi | P10127 |
Miscellaneous databases
PROi | PR:P10127 |
RNActi | P10127, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR018211, ADH_Fe_CS IPR039697, Alcohol_dehydrogenase_Fe |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
PROSITEi | View protein in PROSITE PS00913, ADH_IRON_1, 1 hit PS00060, ADH_IRON_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADH4_YEAST | |
Accessioni | P10127Primary (citable) accession number: P10127 Secondary accession number(s): D6VV79 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 22, 2008 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families