UniProtKB - P10126 (EF1A1_MOUSE)
Elongation factor 1-alpha 1
Eef1a1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 21 | GTPBy similarity | 8 | |
Nucleotide bindingi | 91 – 95 | GTPBy similarity | 5 | |
Nucleotide bindingi | 153 – 156 | GTPBy similarity | 4 |
GO - Molecular functioni
- calmodulin binding Source: CAFA
- drug binding Source: Ensembl
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- identical protein binding Source: MGI
- kinase binding Source: MGI
- mRNA binding Source: MGI
- protein kinase binding Source: MGI
- translation elongation factor activity Source: GO_Central
- tRNA binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- cellular response to drug Source: Ensembl
- cellular response to epidermal growth factor stimulus Source: UniProtKB
- cellular response to nerve growth factor stimulus Source: Ensembl
- negative regulation of reactive oxygen species biosynthetic process Source: MGI
- positive regulation of cell death Source: MGI
- positive regulation of neuron projection development Source: MGI
- regulation of D-erythro-sphingosine kinase activity Source: UniProtKB
- translation Source: GO_Central
- translational elongation Source: GO_Central
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-156842, Eukaryotic Translation Elongation R-MMU-3371511, HSF1 activation R-MMU-6798695, Neutrophil degranulation R-MMU-8876725, Protein methylation |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-alpha 1Short name: EF-1-alpha-1 Alternative name(s): Elongation factor Tu Short name: EF-Tu Eukaryotic elongation factor 1 A-1 Short name: eEF1A-1 |
Gene namesi | Name:Eef1a1 Synonyms:Eef1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096881, Eef1a1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Nucleus
Other locations
- Cytoplasm 1 Publication
Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens. Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B.By similarity
Cytoskeleton
- cortical actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
Plasma Membrane
- plasma membrane Source: MGI
- ruffle membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000090886 | 2 – 462 | Elongation factor 1-alpha 1Add BLAST | 461 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N,N,N-trimethylglycineBy similarity | 1 | |
Modified residuei | 36 | N6,N6,N6-trimethyllysine; alternateBy similarity | 1 | |
Modified residuei | 36 | N6,N6-dimethyllysine; alternateBy similarity | 1 | |
Modified residuei | 36 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 55 | N6,N6-dimethyllysineBy similarity | 1 | |
Modified residuei | 79 | N6,N6,N6-trimethyllysine; by EEF1AKMT1By similarity | 1 | |
Modified residuei | 165 | N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication | 1 | |
Modified residuei | 165 | N6,N6-dimethyllysine; alternate; by EEF1AKMT3By similarity | 1 | |
Modified residuei | 165 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 165 | N6-methyllysine; alternate; by EEF1AKMT3By similarity | 1 | |
Modified residuei | 172 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 273 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 300 | Phosphoserine; by TGFBR1By similarity | 1 | |
Modified residuei | 301 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 318 | N6,N6,N6-trimethyllysine; by EEF1AKMT2By similarity | 1 | |
Modified residuei | 374 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 392 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 392 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 432 | Phosphothreonine; by PASKBy similarity | 1 | |
Modified residuei | 439 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3787 |
EPDi | P10126 |
jPOSTi | P10126 |
PaxDbi | P10126 |
PeptideAtlasi | P10126 |
PRIDEi | P10126 |
TopDownProteomicsi | P10126 |
2D gel databases
SWISS-2DPAGEi | P10126 |
PTM databases
iPTMneti | P10126 |
PhosphoSitePlusi | P10126 |
SwissPalmi | P10126 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037742, Expressed in forelimb bud and 92 other tissues |
ExpressionAtlasi | P10126, baseline and differential |
Genevisiblei | P10126, MM |
Interactioni
Subunit structurei
Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA.
Interacts with PARP1 and TXK.
Interacts with KARS1. May interact with ERGIC2.
Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). May interact with ERGIC2 (By similarity).
Interacts with IFIT1 (via TPR repeats 4-7) (PubMed:19856081).
Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation.
Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (By similarity).
Interacts with PPP1R16B (By similarity).
Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity (By similarity).
By similarity1 PublicationBinary interactionsi
P10126
With | #Exp. | IntAct |
---|---|---|
TSPY1 [Q01534] from Homo sapiens. | 5 | EBI-773865,EBI-1973142 |
GO - Molecular functioni
- calmodulin binding Source: CAFA
- identical protein binding Source: MGI
- kinase binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199385, 160 interactors |
DIPi | DIP-46609N |
IntActi | P10126, 68 interactors |
MINTi | P10126 |
STRINGi | 10090.ENSMUSP00000042457 |
Miscellaneous databases
RNActi | P10126, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 242 | tr-type GAdd BLAST | 238 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 21 | G1By similarity | 8 | |
Regioni | 70 – 74 | G2By similarity | 5 | |
Regioni | 91 – 94 | G3By similarity | 4 | |
Regioni | 153 – 156 | G4By similarity | 4 | |
Regioni | 194 – 196 | G5By similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0052, Eukaryota |
GeneTreei | ENSGT00950000183029 |
HOGENOMi | CLU_007265_3_5_1 |
InParanoidi | P10126 |
OMAi | ICAINKM |
OrthoDBi | 1150082at2759 |
PhylomeDBi | P10126 |
TreeFami | TF300304 |
Family and domain databases
HAMAPi | MF_00118_A, EF_Tu_A, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004539, Transl_elong_EF1A_euk/arc IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00483, EF-1_alpha, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YZ68 | D3YZ68_MOUSE | Elongation factor 1-alpha 1 | Eef1a1 | 249 | Annotation score: | ||
D3Z3I8 | D3Z3I8_MOUSE | Elongation factor 1-alpha 1 | Eef1a1 | 186 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | H → R in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 15 | H → L in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 23 | T → S in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 77 – 78 | LW → QR in CAA31957 (PubMed:2911475).Curated | 2 | |
Sequence conflicti | 83 | S → A in CAA27324 (PubMed:3960725).Curated | 1 | |
Sequence conflicti | 91 – 92 | DA → ES in CAA31957 (PubMed:2911475).Curated | 2 | |
Sequence conflicti | 108 | Q → R in AAA37538 (PubMed:3481036).Curated | 1 | |
Sequence conflicti | 156 | D → G in BAC28085 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 222 | S → H in AAA50406 (PubMed:3215517).Curated | 1 | |
Sequence conflicti | 224 – 226 | SGT → VAP in CAA31957 (PubMed:2911475).Curated | 3 | |
Sequence conflicti | 225 | G → D in AAH04005 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 239 | Missing in CAA31957 (PubMed:2911475).Curated | 1 | |
Sequence conflicti | 350 | P → T in BAC36446 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22432 mRNA Translation: AAA50406.1 X13661 mRNA Translation: CAA31957.1 AK032914 mRNA Translation: BAC28085.1 AK076696 mRNA Translation: BAC36446.1 AK081725 mRNA Translation: BAC38311.1 AK083361 mRNA Translation: BAC38884.1 BC004005 mRNA Translation: AAH04005.1 BC004067 mRNA Translation: AAH04067.1 BC005660 mRNA Translation: AAH05660.1 BC018223 mRNA Translation: AAH18223.1 BC018485 mRNA Translation: AAH18485.1 BC083069 mRNA Translation: AAH83069.1 M17878 Genomic DNA Translation: AAA37538.1 X03688 mRNA Translation: CAA27324.1 |
CCDSi | CCDS40703.1 |
PIRi | S02114, EFMS1 |
RefSeqi | NP_034236.2, NM_010106.2 |
Genome annotation databases
Ensembli | ENSMUST00000042235; ENSMUSP00000042457; ENSMUSG00000037742 |
GeneIDi | 13627 |
KEGGi | mmu:13627 |
UCSCi | uc009qun.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22432 mRNA Translation: AAA50406.1 X13661 mRNA Translation: CAA31957.1 AK032914 mRNA Translation: BAC28085.1 AK076696 mRNA Translation: BAC36446.1 AK081725 mRNA Translation: BAC38311.1 AK083361 mRNA Translation: BAC38884.1 BC004005 mRNA Translation: AAH04005.1 BC004067 mRNA Translation: AAH04067.1 BC005660 mRNA Translation: AAH05660.1 BC018223 mRNA Translation: AAH18223.1 BC018485 mRNA Translation: AAH18485.1 BC083069 mRNA Translation: AAH83069.1 M17878 Genomic DNA Translation: AAA37538.1 X03688 mRNA Translation: CAA27324.1 |
CCDSi | CCDS40703.1 |
PIRi | S02114, EFMS1 |
RefSeqi | NP_034236.2, NM_010106.2 |
3D structure databases
SMRi | P10126 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199385, 160 interactors |
DIPi | DIP-46609N |
IntActi | P10126, 68 interactors |
MINTi | P10126 |
STRINGi | 10090.ENSMUSP00000042457 |
PTM databases
iPTMneti | P10126 |
PhosphoSitePlusi | P10126 |
SwissPalmi | P10126 |
2D gel databases
SWISS-2DPAGEi | P10126 |
Proteomic databases
CPTACi | non-CPTAC-3787 |
EPDi | P10126 |
jPOSTi | P10126 |
PaxDbi | P10126 |
PeptideAtlasi | P10126 |
PRIDEi | P10126 |
TopDownProteomicsi | P10126 |
Genome annotation databases
Ensembli | ENSMUST00000042235; ENSMUSP00000042457; ENSMUSG00000037742 |
GeneIDi | 13627 |
KEGGi | mmu:13627 |
UCSCi | uc009qun.1, mouse |
Organism-specific databases
CTDi | 1915 |
MGIi | MGI:1096881, Eef1a1 |
Phylogenomic databases
eggNOGi | KOG0052, Eukaryota |
GeneTreei | ENSGT00950000183029 |
HOGENOMi | CLU_007265_3_5_1 |
InParanoidi | P10126 |
OMAi | ICAINKM |
OrthoDBi | 1150082at2759 |
PhylomeDBi | P10126 |
TreeFami | TF300304 |
Enzyme and pathway databases
Reactomei | R-MMU-156842, Eukaryotic Translation Elongation R-MMU-3371511, HSF1 activation R-MMU-6798695, Neutrophil degranulation R-MMU-8876725, Protein methylation |
Miscellaneous databases
BioGRID-ORCSi | 13627, 9 hits in 18 CRISPR screens |
ChiTaRSi | Eef1a1, mouse |
PROi | PR:P10126 |
RNActi | P10126, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037742, Expressed in forelimb bud and 92 other tissues |
ExpressionAtlasi | P10126, baseline and differential |
Genevisiblei | P10126, MM |
Family and domain databases
HAMAPi | MF_00118_A, EF_Tu_A, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004539, Transl_elong_EF1A_euk/arc IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00483, EF-1_alpha, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF1A1_MOUSE | |
Accessioni | P10126Primary (citable) accession number: P10126 Secondary accession number(s): Q61511 Q99KU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 25, 2004 | |
Last modified: | February 10, 2021 | |
This is version 219 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families