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Protein

Elongation factor 1-alpha 1

Gene

Eef1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi91 – 95GTPBy similarity5
Nucleotide bindingi153 – 156GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: CAFA
  • drug binding Source: MGI
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • mRNA binding Source: MGI
  • protein kinase binding Source: MGI
  • translation elongation factor activity Source: GO_Central
  • tRNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3371511 HSF1 activation
R-MMU-6798695 Neutrophil degranulation
R-MMU-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
Elongation factor Tu
Short name:
EF-Tu
Eukaryotic elongation factor 1 A-1
Short name:
eEF1A-1
Gene namesi
Name:Eef1a1
Synonyms:Eef1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1096881 Eef1a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000908862 – 462Elongation factor 1-alpha 1Add BLAST461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycineBy similarity1
Modified residuei36N6,N6,N6-trimethyllysine; alternateBy similarity1
Modified residuei36N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei36N6-methyllysine; alternateBy similarity1
Modified residuei55N6,N6-dimethyllysineBy similarity1
Modified residuei79N6,N6,N6-trimethyllysine; by EEF1AKMT1By similarity1
Modified residuei165N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication1
Modified residuei165N6,N6-dimethyllysine; alternate; by EEF1AKMT3By similarity1
Modified residuei165N6-acetyllysine; alternateCombined sources1
Modified residuei165N6-methyllysine; alternate; by EEF1AKMT3By similarity1
Modified residuei172N6-acetyllysineCombined sources1
Modified residuei273N6-acetyllysineCombined sources1
Modified residuei300Phosphoserine; by TGFBR1By similarity1
Modified residuei3015-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei318N6,N6,N6-trimethyllysine; by EEF1AKMT2By similarity1
Modified residuei3745-glutamyl glycerylphosphorylethanolamine1 Publication1
Modified residuei392N6-acetyllysine; alternateCombined sources1
Modified residuei392N6-succinyllysine; alternateCombined sources1
Modified residuei432Phosphothreonine; by PASKBy similarity1
Modified residuei439N6-acetyllysineCombined sources1

Post-translational modificationi

ISGylated.1 Publication
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2.By similarity
Trimethylated at Lys-79 by EEF1AKMT1 (By similarity). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (By similarity). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (By similarity).By similarity1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP10126
PaxDbiP10126
PeptideAtlasiP10126
PRIDEiP10126
TopDownProteomicsiP10126

2D gel databases

SWISS-2DPAGEiP10126

PTM databases

iPTMnetiP10126
PhosphoSitePlusiP10126
SwissPalmiP10126

Expressioni

Gene expression databases

BgeeiENSMUSG00000037742 Expressed in 63 organ(s), highest expression level in female gonad
ExpressionAtlasiP10126 baseline and differential
GenevisibleiP10126 MM

Interactioni

Subunit structurei

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS. May interact with ERGIC2 (By similarity). Interacts with IFIT1 (via TPR repeats 4-7) (PubMed:19856081). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (By similarity). Interacts with PPP1R16B (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TSPY1Q015345EBI-773865,EBI-1973142From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199385, 57 interactors
DIPiDIP-46609N
IntActiP10126, 66 interactors
MINTiP10126
STRINGi10090.ENSMUSP00000042457

Structurei

3D structure databases

ProteinModelPortaliP10126
SMRiP10126
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 242tr-type GAdd BLAST238

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni70 – 74G2By similarity5
Regioni91 – 94G3By similarity4
Regioni153 – 156G4By similarity4
Regioni194 – 196G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00910000144046
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiP10126
KOiK03231
OMAiPAKETKM
OrthoDBiEOG091G05LW
PhylomeDBiP10126
TreeFamiTF300304

Family and domain databases

HAMAPiMF_00118_A EF_Tu_A, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004539 Transl_elong_EF1A_euk/arc
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00483 EF-1_alpha, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P10126-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Length:462
Mass (Da):50,114
Last modified:October 25, 2004 - v3
Checksum:i71072871DE7405DC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YZ68D3YZ68_MOUSE
Elongation factor 1-alpha 1
Eef1a1
249Annotation score:
D3Z3I8D3Z3I8_MOUSE
Elongation factor 1-alpha 1
Eef1a1
186Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7H → R in AAA37538 (PubMed:3481036).Curated1
Sequence conflicti15H → L in AAA37538 (PubMed:3481036).Curated1
Sequence conflicti23T → S in AAA37538 (PubMed:3481036).Curated1
Sequence conflicti77 – 78LW → QR in CAA31957 (PubMed:2911475).Curated2
Sequence conflicti83S → A in CAA27324 (PubMed:3960725).Curated1
Sequence conflicti91 – 92DA → ES in CAA31957 (PubMed:2911475).Curated2
Sequence conflicti108Q → R in AAA37538 (PubMed:3481036).Curated1
Sequence conflicti156D → G in BAC28085 (PubMed:16141072).Curated1
Sequence conflicti222S → H in AAA50406 (PubMed:3215517).Curated1
Sequence conflicti224 – 226SGT → VAP in CAA31957 (PubMed:2911475).Curated3
Sequence conflicti225G → D in AAH04005 (PubMed:15489334).Curated1
Sequence conflicti239Missing in CAA31957 (PubMed:2911475).Curated1
Sequence conflicti350P → T in BAC36446 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22432 mRNA Translation: AAA50406.1
X13661 mRNA Translation: CAA31957.1
AK032914 mRNA Translation: BAC28085.1
AK076696 mRNA Translation: BAC36446.1
AK081725 mRNA Translation: BAC38311.1
AK083361 mRNA Translation: BAC38884.1
BC004005 mRNA Translation: AAH04005.1
BC004067 mRNA Translation: AAH04067.1
BC005660 mRNA Translation: AAH05660.1
BC018223 mRNA Translation: AAH18223.1
BC018485 mRNA Translation: AAH18485.1
BC083069 mRNA Translation: AAH83069.1
M17878 Genomic DNA Translation: AAA37538.1
X03688 mRNA Translation: CAA27324.1
CCDSiCCDS40703.1
PIRiS02114 EFMS1
RefSeqiNP_034236.2, NM_010106.2
UniGeneiMm.138471
Mm.335315
Mm.360075
Mm.391071
Mm.485669
Mm.491424

Genome annotation databases

EnsembliENSMUST00000042235; ENSMUSP00000042457; ENSMUSG00000037742
GeneIDi13627
KEGGimmu:13627
UCSCiuc009qun.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22432 mRNA Translation: AAA50406.1
X13661 mRNA Translation: CAA31957.1
AK032914 mRNA Translation: BAC28085.1
AK076696 mRNA Translation: BAC36446.1
AK081725 mRNA Translation: BAC38311.1
AK083361 mRNA Translation: BAC38884.1
BC004005 mRNA Translation: AAH04005.1
BC004067 mRNA Translation: AAH04067.1
BC005660 mRNA Translation: AAH05660.1
BC018223 mRNA Translation: AAH18223.1
BC018485 mRNA Translation: AAH18485.1
BC083069 mRNA Translation: AAH83069.1
M17878 Genomic DNA Translation: AAA37538.1
X03688 mRNA Translation: CAA27324.1
CCDSiCCDS40703.1
PIRiS02114 EFMS1
RefSeqiNP_034236.2, NM_010106.2
UniGeneiMm.138471
Mm.335315
Mm.360075
Mm.391071
Mm.485669
Mm.491424

3D structure databases

ProteinModelPortaliP10126
SMRiP10126
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199385, 57 interactors
DIPiDIP-46609N
IntActiP10126, 66 interactors
MINTiP10126
STRINGi10090.ENSMUSP00000042457

PTM databases

iPTMnetiP10126
PhosphoSitePlusiP10126
SwissPalmiP10126

2D gel databases

SWISS-2DPAGEiP10126

Proteomic databases

EPDiP10126
PaxDbiP10126
PeptideAtlasiP10126
PRIDEiP10126
TopDownProteomicsiP10126

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042235; ENSMUSP00000042457; ENSMUSG00000037742
GeneIDi13627
KEGGimmu:13627
UCSCiuc009qun.1 mouse

Organism-specific databases

CTDi1915
MGIiMGI:1096881 Eef1a1

Phylogenomic databases

eggNOGiKOG0052 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00910000144046
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiP10126
KOiK03231
OMAiPAKETKM
OrthoDBiEOG091G05LW
PhylomeDBiP10126
TreeFamiTF300304

Enzyme and pathway databases

ReactomeiR-MMU-3371511 HSF1 activation
R-MMU-6798695 Neutrophil degranulation
R-MMU-8876725 Protein methylation

Miscellaneous databases

PROiPR:P10126
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037742 Expressed in 63 organ(s), highest expression level in female gonad
ExpressionAtlasiP10126 baseline and differential
GenevisibleiP10126 MM

Family and domain databases

HAMAPiMF_00118_A EF_Tu_A, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004539 Transl_elong_EF1A_euk/arc
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00483 EF-1_alpha, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_MOUSE
AccessioniPrimary (citable) accession number: P10126
Secondary accession number(s): Q61511
, Q6ZWN2, Q8BMB8, Q8BVS8, Q99KU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 25, 2004
Last modified: November 7, 2018
This is version 201 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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